Actividad antimicrobiana de hongos endofíticos aislados de Brugmansia suaveolens Bercht. & J. Presl

Natural products produced from endophyte fungi have a broad spectrum of biological activity and can be grouped into several categories. Antimicrobials constitute an important group of therapeutic agents, which can be produced and obtained from living organisms. The main objective of this study is to investigate the endophytic fungi and the leaves of Brugmansia suaveolens Bercht. & J. Presl as elicitors, aiming at the production of bioactive substances with antimicrobial properties. The extracts were prepared from 19 fungi isolated from the leaves of B. suaveolens Bercht. & J. Presl, used as a biotic elicitor. The extracts were obtained by fermentation in a submerged medium with the addition of leaves from its dry leaves, and the antimicrobial activity of its extracts was evaluated using the agar diffusion method. The results showed that 57% of the endophytic fungi showed antimicrobial activity after fermentation with the elicitor. It was observed that the antimicrobial activity was increased compared to control cultures. The leaves of B. suaveolens Bercht. & J. Presl presented bioactives that stimulated the production of a substance with antimicrobial activity by endophytic fungi, the study of such compounds showed promise to clarify the application potential of these bioactives.

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Antimicrobial Activity of Endophytic Fungi Isolated from Swietenia macrophylla Leaves

Natural Product Communications ◽

10.1177/1934578x1400900229 ◽

2014 ◽

Vol 9 (2) ◽

pp. 1934578X1400900 ◽

Cited By ~ 1

Author(s):

Darah Ibrahim ◽

Chong Chai Lee ◽

Lim Sheh-Hong

Keyword(s):

Antimicrobial Activity ◽

Endophytic Fungi ◽

Leaf Age ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Swietenia Macrophylla ◽

Stages Of Growth ◽

Agar Plug ◽

Fungal Assemblages ◽

Diffusion Assay

The endophytic fungi isolated from leaves of Swietenia macrophylla of different ages were examined for antimicrobial activity. The agar plug diffusion assay was used for primary screening, followed by the disc diffusion method. A total of 461 filamentous endophytic fungi were isolated and cultured to examine their antimicrobial properties. In the primary screen, 315 isolates (68.3%) exhibited activity against at least one of the test pathogenic microorganisms. The percentage of isolates exhibiting antimicrobial activity increased with leaf age. Endophytic fungal assemblages, as well as those isolates exhibiting antimicrobial properties appeared to increase with leaf age. The main antimicrobial compounds were produced extracellularly by the endophytic fungi. The results suggest that healthy leaves at older stages of growth can be a potential source for the isolation of endophytic fungi with antimicrobial properties.

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An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽

10.3390/ma13194383 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4383

Author(s):

Barbara Lapinska ◽

Aleksandra Szram ◽

Beata Zarzycka ◽

Janina Grzegorczyk ◽

Louis Hardan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Antimicrobial Agents ◽

Resin Composite ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Diffusion Method ◽

Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

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Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1427 ◽

2013 ◽

Vol 14 (5) ◽

pp. 924-929 ◽

Cited By ~ 4

Author(s):

Reena Kulshrestha ◽

J Kranthi ◽

P Krishna Rao ◽

Feroz Jenner ◽

V Abdul Jaleel ◽

...

Keyword(s):

Diabetes Mellitus ◽

Antimicrobial Activity ◽

Dental Health ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Test Organism ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Disk Diffusion Method

ABSTRACT Aim The present study was conducted to evaluate the efficacy of commercially available herbal toothpastes against the different periodontopathogens. Materials and methods Six herbal toothpastes that were commonly commercially available were included in the study. Colgate herbal, Babool, Meswak, Neem active, Dabur red toothpastes were tested for the study whereas sterile normal saline was used as control. Antimicrobial efficacies of dentifrices were evaluated against Streptococcus mutans and Actinobacillus actinomycetemcomitans. The antimicrobial properties of dentifrices were tested by measuring the maximum zone of inhibition at 24 hours on the Mueller Hinton Agar media inoculated with microbial strain using disk diffusion method. Each dentifrice was tested at 100% concentration (full strength). Results The study showed that all dentifrices selected for the study were effective against the entire test organism but to varying degree. Neem active tooth paste gave a reading of 25.4 mm as the zone of inhibition which was highest amongst all of the test dentifrices. Colgate Herbal and Meswak dentifrices recorded a larger maximum zone of inhibition, measuring 23 and 22.6 mm respectively, compared to other toothpastes. All other dentifrices showed the zone of inhibition to be between 17 and 19 mm respectively. Conclusion The antibacterial properties of six dentifrices were studied in vitro and concluded that almost all of the dentifrices available commercially had antibacterial properties to some extent to benefit dental health or antiplaque action. How to cite this article Jenner F, Jaleel VA, Kulshrestha R, Maheswar G, Rao PK, Kranthi J. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013;14(5):924-929.

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Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽

10.12688/f1000research.23250.1 ◽

2020 ◽

Vol 9 ◽

pp. 379 ◽

Cited By ~ 1

Author(s):

Dhurva Prasad Gauchan ◽

Pratistha Kandel ◽

Astha Tuladhar ◽

Ashesh Acharya ◽

Upendra Kadel ◽

...

Keyword(s):

Antimicrobial Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Bioactive Compounds ◽

Endophytic Fungi ◽

Diffusion Method ◽

Dry Extract ◽

Brine Shrimp Lethality Assay ◽

Fungal Extracts ◽

Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

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Antimicrobial and larvicidal potential of endophytic fungi isolated from Tragia involucrata Linn.

Annals of Plant Sciences ◽

10.21746/aps.2017.01.002 ◽

2016 ◽

Vol 6 (01) ◽

pp. 1494 ◽

Cited By ~ 1

Author(s):

Ananda Danagoudar ◽

Chandrashekhar G. Joshi* ◽

M.T. Nivya ◽

H.M. Manjunath ◽

Jagadeesha Poyya ◽

...

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Larvicidal Activity ◽

Endophytic Fungi ◽

Ethyl Acetate Extract ◽

Penicillium Citrinum ◽

Diffusion Method ◽

Disc Diffusion Method ◽

The People ◽

Therapeutic Compounds

Endophytic fungi are one of the untapped resources of therapeutic compounds for various diseases. The present study focused on the antimicrobial as well as larvicidal activity of ethyl acetate extract of endophytic fungi isolated from Tragia involucrata. The ethyl acetate extract of Penicillium citrinum CGJ-C1 (GenBank No.KT780618), Penicillium citrinum CGJ-C2 (KP739821), Cladosporium sp. (KP739822), and Cryptendoxyla hypophloia CGJ-D2 (KT780619) was subjected to antimicrobial activity against a panel of microorganisms by disc diffusion method, larvicidal activity against Culex quinquefasciatus. All the extracts showed significant antimicrobial activity against the tested organisms ranging from 8±0.32 to 13±2.11. The extent of activity was comparable to the standard drugs. The larvicidal potential of the endophytes was superior to T.involucrata extract. The larvicidal activity was found to be dose and time dependent with LC50 value ranging from 4.25- 158.06ppm after 24hrs of treatment. This is the first report on the bioactivity of the endophytes isolated from T.involucrata. Further studies on the bio-guided isolation of lead compound will benefit the people suffering from microbe’s related diseases.

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Antimicrobial Activity of Photoactive Cerium Doped Zinc Oxide

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.307.217 ◽

2020 ◽

Vol 307 ◽

pp. 217-222 ◽

Cited By ~ 1

Author(s):

Maishara Syazrinni Rooshde ◽

Wan Rafizah Wan Abdullah ◽

Amie Zaidah Amran ◽

Noradhiha Farahin Ibrahim ◽

Fazilah Ariffin ◽

...

Keyword(s):

Antimicrobial Activity ◽

Uv Light ◽

Light Exposure ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Gram Positive ◽

E Coli ◽

Antifouling Agent ◽

Doped Zno ◽

Zno Powder

Biofouling and biofilms exist as ubiquitous, undesirable accumulation of flora and fauna upon a given substrate when being immersed into an aquatic medium. Therefore, a novel antifouling based materials with the incorporation of nanotechnology has been developed for the prevention of biofouling in its initial stage through photocatalytic treatment. This study investigated the antimicrobial properties of photoactive Cerium (Ce) doped ZnO powder and explores its potential properties for future antifouling application. ZnO nanoparticles was doped with 0.4 mol% Ce was synthesized through the combination of modified citrate gelation technique and solid state sintering. The successful preparation of Ce doped ZnO was confirmed by XRD and SEM. The antimicrobial activity of Ce doped ZnO against E. coli and S. aureus was determined through antibacterial susceptibility test by agar well diffusion method whilst its photocatalytic inactivation efficiency against selected bacteria was analysed through photodegradation testing under UV light irradiation. The findings demonstrated that the synthesized Ce doped ZnO powder exhibited antibacterial effect against Gram-positive bacteria (S. aureus) and excellent photocatalytic efficiency to inactivate both Gram-negative (E. coli) and Gram-positive (S. aureus). 2 g/L of Ce doped ZnO catalyzed the 100% disinfection of both bacteria in 180 min of UV light exposure. Thus, this proved that Ce doped ZnO powder has the potential as efficient antifouling agent.

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Antimicrobial Activity of Cultivable Endophytic and Rhizosphere Fungi Associated with “Mile-a-Minute,” Mikania cordata (Asteraceae)

BioMed Research International ◽

10.1155/2020/5292571 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Pavithra L. Jayatilake ◽

Helani Munasinghe

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Pharmaceutical Products ◽

Diffusion Method ◽

Molecular Characteristics ◽

Disk Diffusion Method ◽

Rhizosphere Fungi

Endophytic and rhizosphere fungi are understood to be aiding the host plant to overcome a range of biotic and abiotic stresses (nutrition depletion, droughts, etc.) hence, they remain to be reservoirs of plethora of natural products with immense use. Consequently, this investigation of endophytic and rhizosphere fungi isolated from Mikania cordata (a perennial vine that is well established in Sri Lanka) for their antimicrobial properties was performed with the aim of future derivation of potential beneficial pharmaceutical products. Leaves, twigs, and roots of M. cordata were utilized to isolate a total of 9 endophytic fungi out of which the highest amount (44%) accounted was from the twigs. A sample of the immediate layer of soil adhering to the root of M. cordata was utilized to isolate 15 rhizosphere fungi. Fusarium equiseti and Phoma medicaginis were endophytes that were identified based on colony and molecular characteristics. The broad spectrum of antimicrobial activity depicted by F. equiseti (MK517551) was found to be significantly greater (p≤0.05, inhibitory against Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) than P. medicaginis (MK517550) (inhibitory against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) as assessed using the Kirby-Bauer disk diffusion method. Trichoderma virens and Trichoderma asperellum were rhizospere fungi that exhibited remarkable antimicrobial properties against the test pathogens chosen for the study. T. asperellum indicated significantly greater bioactivity against all four bacterial pathogens and Candida albicans ATCC 10231 under study. The ranges of minimum inhibitory concentrations (MICs) of the fungi depicting antimicrobial properties were determined. The results obtained suggest that F. equiseti, P. medicaginis, T. asperellum, and T. virens of M. cordata harness bioprospective values as natural drug candidates. This is the first report on isolation and evaluation of the antimicrobial properties of endophytic and rhizosphere fungi of Mikania cordata.

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ONE-POT SYNTHESIS OF 2,4,5-TRIARYLIMIDAZOLES FROM KETO-OXIMES: CHARACTERIZATION AND EVALUATION OF ANTIMICROBIAL ACTIVITY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i9.34741 ◽

2019 ◽

pp. 263-270

Author(s):

KRANTHI KUMAR T ◽

SREENIVASULU R

Keyword(s):

Antimicrobial Activity ◽

Biological Activities ◽

Antimicrobial Properties ◽

Antimycobacterial Activity ◽

Diffusion Method ◽

Current Investigation ◽

One Pot ◽

Fungal Strains ◽

Aryl Aldehydes ◽

Agar Disc

Background and Objective: Imidazole scaffold is pervasive in pharmaceuticals and it possesses diverse type of biological activities, especially triarylimidazole derivatives are biologically prominent molecules which inspired the current investigation. The objective of the work is to synthesize 15 novel 2,4,5-triarylimidazole derivatives and evaluate their antimicrobial and antimycobacterial activity against selected bacterial and fungal strains. Methods: The title compounds 2,4,5-triaryl-imidazole were synthesized from the corresponding aryl aldehydes and keto-oximes through the cyclization to N-hydroxyimidazoles and reduced thermally to the different imidazole derivatives. Agar disc diffusion method is employed for the antimicrobial and antimycobacterial studies. Results: Fifteen novel 2,4,5-triarylimidazoles were synthesized in adequate yields and characterization of the molecules was done by detailed spectral analysis using advanced analytical support. Results disclosed that all the synthesized compounds were exhibiting antimicrobial properties. Compounds 3h, 3g, 3b, and 3m were stated to possess potent antimicrobial properties in the given bacterial and fungal strains. Conclusion: The current investigation results support the antimicrobial and antimycobacterial activity of the synthesized 2,4,5-triarylimidazole derivatives. Further, research is necessary to explore the mechanism involved in the antimicrobial activity.

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Antimicrobial Efficacy of Toothpastes Containing Fluoride against Clinically Isolated Streptococci mutans

Advances in Research ◽

10.9734/air/2020/v21i1030247 ◽

2020 ◽

pp. 19-24

Author(s):

Eze E. Ajaegbu ◽

Calister U. Ezeh ◽

Adaobi J. Dieke ◽

Adaora L. Onuora ◽

Jane I. Ugochukwu

Keyword(s):

Antimicrobial Activity ◽

Dental Caries ◽

Antimicrobial Properties ◽

Antimicrobial Efficacy ◽

Diffusion Method ◽

Oral Diseases ◽

Laboratory Methods ◽

Chemical Substances ◽

Carious Lesions ◽

Agar Well Diffusion Method

Dental caries is one of the prevalent oral diseases which affect all ages. Many chemical substances with antimicrobial properties, are effective against microorganisms causing dental caries. The objective of this study was to check the antimicrobial efficacy of some toothpastes found in Enugu metropolis, Nigeria more specifically against clinically isolated Streptococci mutans. The antimicrobial efficacy of different brands of toothpastes marketed in Enugu metropolis was assayed against Streptococci mutans using the agar well diffusion method. The clinical Streptococci mutans used for this study were taken from carious lesions of patients that attended the clinic at Federal College of Dental Therapy and Technology, Trans Ekulu, Enugu, Nigeria and were isolated and cultivated using traditional laboratory methods. The present study has shown that toothpaste which contains mint extract as herbal constituent and sodium fluoride had the highest antimicrobial activity, followed by toothpastes containing only sodium monofluorophosphate. Herbal toothpaste with mint can be a safe and effective alternative in the prevention of dental caries.

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Screening research of 90% aqueous ethanolic plant extracts antimicrobial activity against Propionobacterium acnes — an etiological factor of acne occurrence

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2020-24(1)-14 ◽

2020 ◽

Vol 24 (1) ◽

pp. 75-79

Author(s):

G.V. Rusko

Keyword(s):

Antimicrobial Activity ◽

Side Effects ◽

Plant Extracts ◽

Antimicrobial Properties ◽

Raw Material ◽

Diffusion Method ◽

Medicinal And Aromatic Plants ◽

Evernia Prunastri ◽

Phytochemical Study ◽

Medicinal Plant Extracts

Annotation. Prolonged antibacterial therapy of acne, mainly by antibiotics of MLS-group (macrolides, lincosamides and streptogramin B), has a number of side effects, the main of which is the emergence of resistant strains. Considering the risks and side effects of traditional etiotropic acne therapy, the use of herbal medicines may be an alternative of this disease treatment. The purpose of this study was to perform a screening study of 90% aqueous-ethanolic medicinal plant extracts antimicrobial activity against P acnes test strains. Antimicrobial activity of 242 aqueous-ethanolic extracts of 183 medicinal and aromatic plants of various organs against two P. acnes strains: sensitive and MLS resistant by HB (Heart Brain) agar diffusion method was studied. Statistical processing of results has been caried by UTHSCSA ImageTool 2.0, Microsoft Office Excel 2011. Eupatorium cannabinum L. and Grindelia squarrosa (Pursh) Dun. aerial parts, Populus nigra L. fruits and needles, Helichrysum arenarium L., Evernia prunastri (L.) Ach. herbs, Calendula officinalis L. flowers, Geranium pratense L. rhizomes and Larix decidua Mill. needles exhibited strong antimicrobial activity (diameter of growth inhibition≥ 15 mm) against test strains. Representatives with antimicrobial properties against acne agents have been found among medicinal plants of Ukraine's flora. The detection of pronounced antimicrobial properties in various pharmacopoeial and non-pharmacopoeial plants necessitates the need for phytochemical study of the raw material to isolate and identify its active components.

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