scholarly journals Mitigating dietary aflatoxins in Wistar rat using selected phyto-antioxidant sources

2021 ◽  
Vol 48 (2) ◽  
pp. 38-52
Author(s):  
E. O. Ewuola ◽  
P. C. Emerue

This study was conducted to investigate the mitigation of dietary aflatoxin in Wistar rats using extract from selected phyto-antioxidant sources. A total of one hundred and twenty Wistar rats weighing between 180-190g (80 females and 40 males) at six weeks old were allotted to eight treatments with 15 rats per treatment (10 females and 5 males), in a completely randomized design. The treatments were Treatment 1 (Normal diet + no extract and no aflatoxin), Treatment 2 (Aflatoxin contaminated diet + no extract), Treatment 3 (Aflatoxin contaminated diet + 100mg/kg BW Carrot extract), Treatment 4 (Aflatoxin contaminated diet + 200mg/kg BW Carrot extract), Treatment 5 (Aflatoxin contaminated diet + 100mg/kg BW Ginger extract), Treatment 6 (Aflatoxin contaminated diet + 200mg/kg BW Ginger extract), Treatment 7 (Aflatoxin contaminated diet +100mg/kg BW Garlic extract), Treatment 8 (Aflatoxin contaminated diet+ 200mg/kg BW Garlic extract). Body weight gain and feed conversion ratio of Wister rats (T8) administered 200 mg/kg BW was significant (p<0.05), with higher weight gain in male (206.00g) and female (199.70g). However, eviscerated and organs weights of both male and female Wistar rats was similar (p>0.05) across the treatments. Haematological and serum biochemical indices among the treatments was not significant (p>0.05), except for the globulin in male Wistar rats that differed significantly (p<0.05) with the value (5.00g/dL) being higher in T7. In conclusion, 200mg/kg body weight of garlic extract improved growth rate of Wistar rats, without any deleterious effect on haematological and serum biochemical parameters. Therefore, 200mg/kg body weight garlic extract mitigated the adverse effect of aflatoxin contaminated feed in Wistar rats.     Cette étude a été menée pour étudier l'atténuation de l'aflatoxine diététique chez les rats de 'Wistar' tout en employant l'extrait des sources phyto-antioxydantes choisies. Un total de cent vingt rats Wistar pesant entre 180 et 190 g (80 femelles et 40 mâles) à l'âge de six semaines ont été attribués à huit traitements avec 15 rats par traitement (10 femelles et 5 mâles), dans une conception complètement randomisée. Les traitements étaient le traitement 1 (régime normal + aucun extrait et aucune aflatoxine), traitement 2 (régime contaminé par l'aflatoxine + aucun extrait), traitement 3 (régime contaminé par l'aflatoxine + extrait de carotte BW de 100mg/kg), traitement 4 (régime contaminé par l'aflatoxine + extrait de carotte BW de 200mg/kg), traitement 5 (Aflatox alimentation contaminée par l'aflatoxine + extrait de gingembre BW 100mg/kg), Traitement 6 (régime contaminé à l'aflatoxine + extrait de gingembre BW 200mg/kg), Traitement 7 (régime contaminé par l'aflatoxine +100mg/kg extrait d'ail BW), Traitement 8 (régime contaminé à l'aflatoxine+ 200mg/kg extrait d'ail BW). Le gain de poids corporel et le rapport de conversion des aliments pour animaux des rats wisters (T8) administrés 200 mg/kg BW étaient significatifs (p<0.05), avec un gain de poids plus élevé chez les rats Wistar mâles (206.00 g) et femelles (199.70 g). Cependant, les poids éviscérés et organes des rats Wistar mâles et femelles étaient similaires (p>0.05) à travers les traitements. Les indices biochimiques hématologiques et sériques parmi les traitements n'étaient pas significatifs (p>0.05), à l'exception de la globuline chez les rats wistar mâles qui différait considérablement (p<0.05) avec la valeur (5.00 g/dL) étant plus élevée dans T7. En conclusion, le poids corporel de 200mg/kg de l'extrait d'ail a amélioré le taux de croissance des rats de Wistar, sans n'importe quel effet délétère sur les paramètres biochimiques hématologiques et sériques. Par conséquent, l'extrait d'ail de poids corporel de 200mg/kg a atténué l'effet défavorable de l'alimentation contaminée d'aflatoxin chez les rats de Wistar.

Author(s):  
Vijayalingam Thavasi Alagan ◽  
Rajesh Nakulan Vatsala ◽  
Ilavarasan Sagadevan ◽  
Vairamuthu Subbiah ◽  
Venkataramanan Ragothaman

Abstract Background An effort was made to assess the effectiveness of dietary supplementation of Ulva lactuca (U. lactuca), a seaweed and Azolla individually and in combination on different physiological parameters of Aseel chicken. A total of 40 Aseel chicks of 8 weeks old were allocated into four groups, C1 (control), T1 (Azolla alone), T2 (seaweed alone) and T3 (seaweed plus Azolla). Aseel chicks in C1 were fed with formulated grower feed alone, birds in T1 had control diet with 5% Azolla, birds in T2 had control diet with 3% U. lactuca and birds in T3 had control diet admixtured with 5% of Azolla and 3% of U. lactuca. Parameters were recorded for a continuous period of 2 months in 15 days interval. Results The growth performance was found to be significant (P ≤ 0.05) during 30 days of feed trials and highly significant (P ≤ 0.01) during 45 and 60 days of treatment. Birds in T3 had a higher body weight gain, shank length and feed conversion efficiency followed with T1 and T2 compared to C1. PCV, RBC, WBC and heterophil counts were not influenced (P ≥ 0.05) by dietary treatments. Uric acid, creatinine, AST, glucose, triglycerides and magnesium levels revealed a high significant (P ≤ 0.01) variation, and cholesterol level showed significant (P ≤ 0.05) changes during 30th and 60th day of feed trials between the control and treatment groups. Total protein, globulin, phosphorus and electrolytes like Na, K and Cl levels were not significant (P ≥ 0.05) during 30th day and were highly significant (P ≤ 0.01) during the 60th day of the trial. Conclusions It could be noted that inclusion of U. lactuca and Azolla as feed supplement in grower chicken had a better body weight gain when given in combination (U. lactuca and Azolla) rather than supplemented with the sole entity. Based on the haematological and serum biochemical analysis, the supplementation of U. lactuca and Azolla at the levels included in this trial did not pose any threat to the physiological well-being of Aseel chicken.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1511
Author(s):  
Joseph P. Gulizia ◽  
Kevin M. Downs

Two trials were conducted to determine feed color effects on broiler performance. A completely randomized design was used. Trial 1 included four treatments: control (complete broiler starter diet), red, green, and blue; and Trial 2 included four treatments: control, orange, yellow, and purple. Each trial had 4 treatments with 4 replicates (60 birds/treatment) fed to 240 male Cobb 500 broilers during a 21 d grow out. Data were analyzed using the GLM procedure. In Trial 1, there were no treatment effects on average body weight, body weight gain, and feed consumption (p > 0.05). Adjusted feed conversion for control (1.23) was less than red (1.27; p = 0.001) and green (1.26; p = 0.009), with blue (1.25; p = 0.056) tending to be different during the experimental period. In Trial 2, there were no treatment effects on average body weight, feed consumption, and adjusted feed conversion during this study (p > 0.05). Body weight gain between d 1 to 14 for purple (490.78 g/bird) was more than orange (467 g/bird; p = 0.013) and yellow (461 g/bird; p= 0.004), with control (474 g/bird; p = 0.052) tending to be different. Results indicate that these feed colors had some, albeit limited, influence on broiler performance parameters.


2017 ◽  
Vol 9 (1) ◽  
pp. 71-76 ◽  
Author(s):  
M. Amri

The study effect of Fermented Palm Kernel Cage (FPKC) in feed on growth of Cyprinus carpio L. was done to evaluate the utilization of palm kernel cage increasing the quality by fermentation technology as a feed in ration to Cyprinus carpio L. performance and get a good level in ration. This experiment was set in a Randomized Completely Design with four treatments and four replicates.  Treatments were diets with : A (12% PKC), B (15% FPKC),  C (18% FPKC), and D (21% FPKC). Results of experiment indicated that the feed consumption body weight gain, feed conversion and income over feed cost was in proved by 18% FPKC in the diet.


2020 ◽  
Vol 7 (2) ◽  
pp. 133
Author(s):  
Hamdan Has ◽  
Astriana Napirah ◽  
Widhi Kurniawan ◽  
La Ode Nafiu ◽  
Takdir Saili

ABSTRAKPenggunaan acidifier baik organik atau sintetis dapat meningkatkan optimalisasi nutrien di dalam saluran pencernaan.  Optimalisasi nutrien diharapkan dapat meningkatkan performa ternak khususnya puyuh. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan dua macam acidifier terhadap performa puyuh fase grower. 100 ekor unsexed puyuh (Coturnix coturnix Japonica) fase grower umur 14-40 hari digunakan dalam penelitian yang menggunakan rancangan acak lengkap dengan lima perlakuan dan empat ulangan. Bahan pakan yang digunakan adalah jagung kuning, dedak, konsentrat puyuh komersil, sari belimbing wuluh, dan asam sitrat sintetis. Perlakuan yang dicobakan terdiri dari perlakuan kontrol (P0), penggunaan 0,3% asam sitrat sintetis (P1), penggunaan 0,25% sari belimbing wuluh (P2), penggunaan 0,6% asam sitrat sintetis (P3), dan penggunaan 0,5% sari belimbing wuluh (P4). Variabel yang diamati adalah konsumsi ransum, pertambahan bobot badan dan konversi ransum mingguan. Penggunaan sari belimbing wuluh memiliki konsumsi pakan yang lebih rendah  (P<0,05) pada minggu pertama dibanding kontrol dan asam sitrat sintetis.  Penggunaan asam sitrat sintetis meningkatkan pertambahan bobot badan pada minggu pertama (P<0,05). Penggunaan asam sitrat sintetis nyata dapat menurunkan konversi pakan minggu ke tiga dan empat dibanding kontrol dan asam organik belimbing wuluh. Kesimpulan penelitian ini yaitu penggunaan sari belimbing wuluh sebagai acidifier belum menunjukkan perbaikan performa yang signifikan sedangkan penggunaan asam sitrat sintetis memiliki performa yang lebih baik dibanding perlakuan kontrol dan penggunaan sari belimbing wuluh.Kata kunci: acidifier, asam sitrat, belimbing wuluh, puyuh fase growerABSTRACTThe organic and synthetic acidifiers could improve the optimization of nutrients utilization in the quail digestive tract. Furthermore, the optimization of nutrients is expected to improve quail performance. This study was aimed to determine the effect of using two types of acidifiers (Averrhoa bilimbi juice and synthetic citric acid) on the grower phase of quail performance. Total of 100 unsexed quails (Coturnix coturnix japonica) grower phase aged 14-40 days were used in this research and designed as a completely randomized design of five treatments and four replications. Feed ingredients used were yellow corn, rice bran, commercial quail concentrate, Averrhoa bilimbi juice (organic acidifier), and synthetic citric acid. The treatments consisted of control (P0), 0.3% synthetic citric acid (P1), use of 0.25% Averrhoa bilimbi juice (P2), use of 0.6% synthetic citric acid (P3), and use of 0.5% Averrhoa bilimbi juice (P4). The variables observed were weekly feed intake, body weight gain, and feed conversion. The result showed that utilization of Averrhoa bilimbi juice has lower feed consumption (P<0.05) in the first week compare to control and synthetic citric acid, and synthetic citric acid utilization increases body weight gain in the first week (P<0.05). The use of synthetic citric acid significantly reduces feed conversion in the third and fourth weeks compared to control and organic acid groups. The conclusion of this study is the utilization of Averrhoa bilimbi juice as an acidifier has not shown significant improvements in quail performance. The use of synthetic citric acid has a better performance compared to control and Averrhoa bilimbi juice utilization.Keywords: acidifier, organic acidifier, citric acid, growing quail


Author(s):  
Jay Narayan Shrestha ◽  
Prabha Niraula

 Best diet of the fish is the sole of production of aquaculture. Growth performance of Clarias gariepinus on the basis of formulated feed supply was carried out from 7th March to 6th May, 2019 in the laboratory of P.G.Campus, Biratnagar in which 130 juveniles (each of 10±0.11 g.) of catfish were cultured in 13 aquaria feeding with treatment diets having crude protein level 35%, 40%, 45% and 50% for treatment and commercial diet for control. There are no significant differences between the water quality parameters and body weight gain (p>0.05) in all treatments. The highest body weight gain (7.31±0.11 g; 65.20%) and SGR (13.051±1.15 g) was observed in fish fed with T C (45% protein level). This value was lowered than value of control(C) i.e. 7.59±0 g; 69.88% and 13.55±0 g respectively. Feed conversion ratio (FCR) was best (13.28±1.89 g) in T D (50% protein level feed). Its value was larger (7.52±1.11 g) in T C than C (7.15±0 g). Mortality rate was highest in T C and C i.e. 30% and lowest in T A and T B i.e. 23.33%. Weight gain of the fish increased with increasing protein level in diet till crude protein level 45% then it was dropped at the diet with protein level 50%. The best weight gain was observed in 7th week having crude protein level 45%. The diet with protein level 45% is suggested for farmers concerning the fish (Clarias gariepinus) in the aquaculture.


ZOOTEC ◽  
2018 ◽  
Vol 39 (1) ◽  
pp. 42
Author(s):  
Agung R. P. Rumondor ◽  
B. Tulung ◽  
A. Rumambi ◽  
C.A Rahasia

EFFECT OF REPLACEMENT OF CORN WITH SORGUM Cv. KAWALI IN PELLET RANSUM ON LOCAL RABBIT PERFORM. The purpose of this study was to determine the effect of substitution corn with sorghum in rabbit rations on feed consumption, body weight gain, and ration conversion. The duration of the study was 28 days using 20 local rabbits. The statistical design used in this study was a completely randomized design, with 4 (four) treatment rations and 5 (five) replications. The four experimental rations, namely R0: 45% corn + 0% sorghum, R1: 30% corn + 15% sorghum, R2 15% corn + 30% sorghum, R3 0% corn + 45% sorghum. Variables were measured, viz: feed consumption, body weight gain and ration conversion. The results showed that the average of feed consumption, body weight gain, and feed conversion, were: 28.27–30.59 g /head /day, 15.76–21.55 g / head /day, and 1.42-1.80 g /head /day, respectively. Based on the analysis of variance, the treatments ration had no significant effect (P> 0.05) on consumption, but had a very significant effect (P <0.01) on body weight gain and feed conversion. The LSD test showed that all treatment rations using sorghum (R1, R2, and R3) gave results of body weight gain which were very significantly different (P <0.01) and higher than R0. Whereas, on ration conversion, treatments R1, R2, and R3 give very different results (P <0.01) lower than R0. It is concluded that the substitution of corn with sorghum at the level of 45% in the ration gives the highest results for body weight gain and conversion of rations for rabbits. Keywords: corn, sorghum, ration, performance, local rabbit


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ganesha Ade Riemas ◽  
Iman Hernaman ◽  
Diky Ramdani ◽  
Bambang Nurhadi

ABSTRAK. Tujuan penelitian ini untuk mengukur seberapa besar pengaruh minyak ikan yang sudah terenkapsulasi terhadap kolesterol darah dan performa pada Domba. Penelitian telah dilakukan di Sub Unit Pelayanan Pengembangan Pembibitan Ternak Domba dan Kambing (SUPPPTDK) Bunihayu, Subang pada tanggal 20 Januari 2020 sampai 27 Maret 2020. Sebanyak 18 ekor domba Ekor Tipis jantan dengan bobot 15,99±0,98 kg dialokasikan ke dalam 3 perlakuan secara acak. Domba tersebut diberi ransum perlakuan yang disuplementasi dengan mikroenkapsulasi minyak ikan sebanyak 0% (P0), 2,5% (P1), dan 5% (P2). Data yang terkumpul dilakukan analisis ragam dan bila hasil berbeda nyata maka dilanjutkan dengan uji Duncan. Hasil menunjukkan bahwa mikroenkapsulasi minyak ikan dapat menurunkan kolesterol darah (P0,05), namun tidak berpengaruh nyata terhadap pertambahan bobot badan, konsumsi dan konversi ransum (P0,05). Penurunan kadar kolesterol terjadi pada kelompok domba dengan perlakuan mikroenkapsulasi sebanyak 2,5% (P1) dan mikroenkapsulasi 5% (P2). Kadar kolesterol darah masing-masing perlakuan berturut-turut adalah 100,70 mg/dl (P0); 96,20 mg/dl (P1); dan 78,76 mg/dl (P2). Rataan yang terbaik pada pertambahan bobot badan, konsumsi bahan kering harian, dan konversi ransum terdapat pada P2 yaitu 63,96 g/hari, 574,13 g/hari, dan 9,08. Hasil penelitian dapat disimpulkan bahwa mikroenkapsulasi minyak ikan dapat menurunkan kolesterol darah, dengan penurunan tertinggi pada pemberian 5%.  (The impact of microencapsulation fish oil in feed on blood cholesterols and performance on sheep) ABSTRAK. This study measured the effect of encapsulated fish oil on blood cholesterol and sheep performance. The study was conducted at the Bunihayu Sheep and Goat Breeding Services Bunihayu, Subang on January 20, 2020 to March 27, 2020. A total of 18 male thin-tailed sheep 15.99±0.98 kg were allocated randomly into 3 treatments. The sheep were given feed supplemented with fish oil microencapsulation of 0% (P0), 2.5% (P1), and 5% (P2). The data were collected and analyzed by analysis of variance and, if the result is significantly different, continued by Duncan's test. The results showed that fish oil microencapsulation decreased blood cholesterol levels (P0.05). However, it did not affect body weight gain, consumption, and feed conversion (P0.05). Decreased levels of cholesterol occurred in the group of sheep with 2.5% microencapsulation (P1) and 5% microencapsulation (P2). Blood cholesterol levels of each treatment were 100.70 mg/dl (P0); 96.20 mg/dl (P1); and 78.76 mg/dl (P2), respectively. Averagely, the highest body weight gain, dry matter intake and feed conversion were found in P2 as much as 63.96 g/day, 574.13 g/day, and 9.08, respectively. The results can be concluded that microencapsulation of fish oil can reduce blood cholesterol, with the highest decrease at the level of 5%.


2021 ◽  
Vol 888 (1) ◽  
pp. 012072
Author(s):  
Siti Khairunnisa ◽  
Sumiati ◽  
Cece Sumantri ◽  
Wiwin Winarsih

Abstract This study aimed to evaluate the effect of zinc supplementation in various diets on the performance and health status of IPB-D2 chickens. Subsequently, about 180 one-week-old IPB-D2 chickens were used in the study and were fed the treatment diets for 12 weeks. Also, this study employed a completely randomised factorial design with six treatments and three replications of ten birds each. These treatment diets included A1, which contained nutrients based on SNI (2013) without Zn supplementation, A2 which is a combination of A1 and 60 ppm of Zinc, B1 which contained nutrients 5% higher than SNI without Zn supplementation, B2 which is a combination of B1 and 60 ppm Zinc, C1 which contains nutrients 10% higher than SNI without Zn supplementation, and lastly, C2 which is a combination of C1 and 60 ppm of Zinc. Furthermore, the data were analysed using ANOVA, and the parameters observed were the body weight, body weight gain, feed consumption, feed conversion ratio, blood profile and the villi surface area of the jejunum. Consequently, the results showed that C2 significantly (P<0.05) increased the body weight and body weight gain when fed to the finisher and grower chickens, respectively. More so, when the finisher chickens were fed the 10% SNI diet, there was an increased body weight gain and also a significantly (P<0.05) decreased feed conversion ratio. Furthermore, the addition of 60 ppm zinc increased haemoglobin significantly (P<0.05), whereas C2 decreased leukocytes significantly (P<0.05) in chicken blood. However, the treatments had no effect on feed consumption in the villi surface area of the jejunum. Therefore, the study concluded that supplementing the IPB-D2 chickens with 60 ppm zinc in a 10% SNI diet resulted in the best performance and health status.


2001 ◽  
Vol 2 (2) ◽  
pp. 26-31
Author(s):  
Al Qudri

ABSTRACT. Tempe of soybean is fermented soybean with Rhizopus ologosporus fungus. From a result of researchers, it is the fact that tempe can increase valuable of nutrion and digest capacity and also produce antibiotic . Utilization of abtibiotic in chiken food as stimulus growth in not recommended because it can cause resistance a certain germ and it is presume there are antibiotic residu in egg and chiken meat. It is hope that the use of fermented soybean oil meal can increase nutrient value and digest capacity as well as produce antibiotic, so that it can get a better growth and over come a problem of using antibiotic in chiken food. The study was conducted to evaluate the effect of fermented soybean oil meal utilization in the ration on body weight gain, feed consumption, feed conversion and mortality. The study use 80 unsex day old chick broiler CP-707 and conducted at Poulty Laboratory Product Agriculture Faculty, Syiah Kuala University. Chiken were putting in 27 houses unit, each unit contain of four chiken. The design of experiment was Completely Randomized Design, four treatments and five replication. The treatments were ration contained 0%, 5%, 10% and 15% fermented soybean oil meal as replace the use of soybean oil meal. The protein and energy content of all dietary treatments were equally composed from six weeks investigation, were body weight gain, feed consumption, feed conversion and mortality. The result of the study indicated that up to 10% graded of fermented soybean oil meal in ration were significant difference influenced increase the body weight gain and decrease the feed conversion. The fermented soybeanoil meal) utilization more than 10% were significant difference decrease the body weight gain and increase the feed conversion. If the inclusion was increase the feed consumption would tendency decline. In this study there were no mortality in all treatment. In can be advised from this study that fermented soybean oil meal could be used in broiler ration or in all poulty ration.


2018 ◽  
Vol 65 (1) ◽  
Author(s):  
E. Prabu ◽  
C.B.T Rajagopalsamy ◽  
B. Ahilan ◽  
Jegan Michael Andro Jeevagan ◽  
M. Renuhadevi

A 60 days indoor growth trial was conducted to study the effect of dietary supplementation of biofloc meal on growth and survival of juvenile GIFT tilapia. Four isonitrogenous and isoenergetic experimental diets (32% crude protein) were prepared using biofloc meal at different inclusion levels viz., 0 (T0), 20 (T1), 30 (T2) and 40% (T3). A commercial diet (T4) was used for comparison. The feeding trial was conducted in 15 nos. of 40 l plastic troughs in triplicate, utilising GIFT tilapias weighing an average of 2 g. During the experimental period, water quality parameters were measured and recorded daily. The mean value of water temperature, pH, dissolved oxygen, salinity, ammonia-N, nitrite-N, nitrate-N, hardness and alkalinity recorded in the experimental systems were 28.5°C, 8.1, 5.5 ppm, 4.5 ppt, 0.03 ppm, 0.07 ppm, 11 ppm, 630 ppm and 162.5 ppm respectively. Among the biofloc meal enriched diets, diet T1 with 20% biofloc yielded the best results in terms of average body weight gain, feed conversion ratio (FCR), specific growth rate (SGR), protein efficiency ratio (PER) and feed efficiency ratio (FER). The mean body weight gain recorded in T1 was 25.28±0.81 g. The results demonstrated that biofloc meal is a potential ingredient that can be incorporated in GIFT tilapia diet at 20% level for better growth performance.


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