scholarly journals Ameliorative Potential of Clerodendrum volubile Ethanol Leaf Extract on Doxorubicin-Induced Hepatorenal Toxicities in Rats

2021 ◽  
Vol 1 (1) ◽  
pp. 1-13
Author(s):  
Adejuwon A. Adeneye ◽  
Olufunke E. Olorundare ◽  
Akinyele O. Akinsola ◽  
Daniel A. Sanni ◽  
James M. Ntambi ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Bilirubin ◽  
Kidney Injury ◽  
Hepatic Function ◽  
Serum Electrolytes ◽  
Histological Studies ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Free Radicals Scavenging

Background and Purpose: Hepatorenal toxicity is a side effect of the anthracycline cytotoxic antibiotics, doxorubicin that is used in cancer treatment. The study investigated the ameliorative potential of Clerodendrum volubile ethanol leaf extract (CVE) on doxorubicin (DOX)-induced hepatorenal toxicities. Methods: Male Wistar rats were pretreated with Clerodendrum volubile ethanol leaf extract (50 - 400 mg/kg/day, p.o) followed by intraperitoneal injection of 2.5 mg/kg of DOX on alternate days for 14 days. Hepatorenal toxicity was assessed using renal function parameters (serum electrolytes, blood urea and creatinine), hepatic function endpoints [aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), total protein (TP), albumin (ALB) and total bilirubin (TB)]. In addition, the antioxidant activity in the kidney and liver tissues were assayed and histological studies of these tissues were also conducted. Results: Oral pretreatment with 50 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day and 400 mg/kg/day of CVE remarkably ameliorated DOX-induced liver and kidney injury by lowering the serum ALT, AST, ALP, Cr and BUN levels. CVE pretreatment remarkably ameliorated DOX-induced increases in the CAT, SOD and GPx activities and MDA levels compared to the DOX-treated rats. The biochemical changes were corroborated by improvements in the DOX-induced histological lesions seen in the hepatic and renal tissues examined. Conclusions: Overall, these findings suggest that Clerodendrum volubile ethanol leaf extract elicits protective effect against DOX-induced hepatorenal toxicities mediated primarily via oxidative stress suppression and improvement in the free radicals scavenging activities of CVE.

scholarly journals Hepato-Renal Activities of Hydro-Methanol Leaf Extract of Cnidoscolus aconitifolius in Adult Male Wistar Rats

2021 ◽  
Vol 11 (4) ◽  
pp. 5-9
Author(s):  
Ijeoma Ezebuiro ◽  
Avweresuoghene Ododo ◽  
Uchechukwu Ifeanyichukwu Apugo
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Protective Agent ◽  
Treatment Groups ◽  
Cnidoscolus Aconitifolius ◽  
Group I ◽  
Male Wistar Rats ◽  
Liver And Kidney

Introduction: Medicinal plants such as Cnidoscolus aconitifolius (CA) have been studied over the years for their protective and curative potentials against a myriad of common global health challenges such as hepatorenal injuries. Objectives: To ascertaining the effects of the hydromethanol leaf extract of CA (HMLECA) on hepato-renal parameters in adult male wistar rats. Method: A total of 18 adult male wistar rats were divided into 3 groups of six rats each. Group I served as the negative control which received distilled water while groups 2 and 3 served as extract treatment groups which received 200 and 400 mg/kg BW of the HMLECA respectively. The administration was daily for a period of 58 days while blood sample for the biochemical analysis was drawn via cardiac puncture at the end of the study following light chloroform anaesthesia. Results and Discussion: The results showed that the administration of both doses of the extract produced no significant (P<0.05) effect on the levels of serum liver enzyme (AST, ALT, ALP), TP, ALB, electrolytes (K+, Na+ and HCO3–), Creatinine and Urea while that of the 400 mg/kg BW produced significant (P<0.05) decrease in the level of TB and Cl –. Hence, the administration of the leaf extract in this study did not elicit any toxic effect on both the liver and kidney functions and could be effective as hepato-renal protective agent. Keywords: Cnidoscolus aconitifolius, Liver function, Liver enzymes, Renal function, Serum biochemicals, Serum electrolytes

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Dimethylnitrosamine (DMN) pre-treated rats and protective effect of Vernonia amygdalina post-treatment on liver function

2016 ◽  
Vol 4 (1) ◽  
pp. 74
Author(s):  
Usunomena Usunobun ◽  
Gabriel Anyanwu
Keyword(s):  
Total Protein ◽  
Leaf Extract ◽  
Total Bilirubin ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Post Treatment ◽  
Vernonia Amygdalina ◽  
Male Wistar Rats ◽  
Drug Treatments ◽  
Ethanolic Leaf Extract

Background: Liver disease and toxicity is common, especially with many drug treatments. The effect of Vernonia amygdalina ethanolic leaf extract post-treatment on rats pre-treated with dimethylnitrosamine (DMN) was evaluated.Methods: Male wistar rats were orally administered DMN (single dose of 25 mg/kg) on first day and thereafter post-treated with 100 and 200mg/kg ethanolic leaf extract of Vernonia amygdalina for seven days. Analysis of serum concentrations of albumin, total protein, total bilirubin and glucose were carried out.Results: Administration of DMN alone to rats significantly increased glucose and total bilirubin concentration (P < 0.05) in the serum while it significantly reduced (P<0.05) serum total protein and albumin concentrations when compared with controls. However, post-treatment of DMN administered rats with 100 and 200 mg/kg ethanolic extract leaf of Vernonia amygdalina significantly (P<0.05) reversed these changes in a concentration dependent manner.Conclusion: In conclusion ethanolic leaf extract of Vernonia amygdalina have a potent hypoglycaemic and hepatoprotective action against dimethylnitrosamine-induced liver damage in rats may be due to its antioxidant properties.

scholarly journals Antihypertensive effect of methanol leaf extract of Anacardium occidentale against L‐Nitro Arginine Methyl Ester (L‐NAME)‐induced hypertension in male Wistar rats

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Adebowale Benard Saba ◽  
Adedeji Kolawole Adebayo ◽  
Ademola Adetokunbo Oyagbemi ◽  
Temidayo Olutayo Omobowale ◽  
Olufunke Eunice Ola-Davies ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Antihypertensive Effect ◽  
Anacardium Occidentale ◽  
Induced Hypertension ◽  
Male Wistar Rats

scholarly journals Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats

2020 ◽  
Vol 8 (2) ◽  
pp. 74-82
Author(s):  
Forough Kajbaf ◽  
Shahrbanoo Oryan ◽  
Ramesh Ahmadi ◽  
Akram Eidi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Diabetic Control ◽  
Albumin Level ◽  
Lower Percentage ◽  
Peganum Harmala ◽  
Diabetic Kidney ◽  
Male Wistar Rats

Background: Growing evidence has shown that the apoptosis of cells plays an important role in the advancement of the Diabetic nephropathy (DN). Objectives: This study attempted to discover the therapeutic potential of Peganum harmala leaf extract in the apoptosis of diabetic kidney disease. Methods: In the present experimental research, 32 male Wistar rats were studied, and diabetes was induced by streptozotocin (STZ) (65 mg/kg). The animals were randomly divided into four groups (n=8, in each group) as follows: control, diabetic, control+leaf extract, diabetic+leaf extract. For our purposes, the methanolic extract of P. harmala leaves (150 mg/kg) was given by gavage for 28 days. Flow cytometry and real-time polymerase chain reaction (PCR) analyses were utilized to determine the percentages of apoptotic cells. Also, histological alterations and blood biochemical parameters were evaluated. Results: The P. harmala leaf extract has a high amount of flavonoids (25.84%), a lower percentage of alkaloids (0.14%), and some antioxidant properties. Serum urea (P<0.001) and apoptosis (P<0.05) significantly elevated in diabetic rats relative to the control ones. The mean of fasting blood creatinine, urea, and albumin level was not significantly changed in diabetic+leaf extract rats as compared to the diabetic ones. Histopathological results also displayed that diabetic complications in the kidney could not be improved following treatment by the leaf extract of P. harmala. In addition, the leaf extract could not significantly reduce the apoptosis and caspase-3 expression compared to diabetics in renal cells. Conclusion: Based on our findings, the leaf extract of P. harmala is unable to inhibit apoptosis in the diabetic kidney model.

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

Sign in / Sign up

Export Citation Format

Share Document