Effectiveness of Guava Leaf Steep water against the bacterial growth of S. Mutans with Microdillution Method

2021 ◽  
pp. 5745-5748
Author(s):  
Cut Aja Nuraskin ◽  
Reca Reca ◽  
Wirza Wirza ◽  
Ainun Mardiah ◽  
Rivansyah Suhendra ◽  
...  
Keyword(s):  
Dental Caries ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Oral Diseases ◽  
Preclinical Trial ◽  
Microdilution Method ◽  
Inhibition Concentration ◽  
Medicinal Properties ◽  
Guava Leaves ◽  
Guava Leaf

Indonesia is famously known for its rich plant biodiversity with medicinal properties. Guava (Psidium guajava Linn.) is one of medicinal plants which can be used as alternative for treating diseases. Guava leaf contains flavonoids, saponins, and tannins, that can act as inhibitors against bacteria, antioxidants, and antibacterial. Numerous dental and oral diseases are caused by bacteria, one of which is dental caries. Principally, dental caries can be prevented by maintaining the oral cavity hygiene, chemically or mechanically, using antiseptics possessing antibacterial activities. The aim of this study was to investigate the effectiveness of guava leaves steep water against S. Mutans bacterium using microdilution method. The aim also included the investigation of inhibition concentration of guava leaves steep water. This research was experimental in nature, by using the guava leaves steep water as the sample with various concentrations (0.753%, 1.563%, 3.125%, 6.25%, 12.5%, 25%, 50%, 100%). S. Mutans was obtained from the laboratory of veterinary Universitas Syiah Kuala (Unsyiah). The results of this research revealed that the guava leaves steep water was effective in inhibiting S. Mutans with Minimum Inhibitory Concentration (MIC) of 6.25% In conclusion, we would like to investigate the guava leaves steep water as a mouthwash in our research with a preclinical trial.

scholarly journals Antibacterial activity of GUAVA, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from Seabob shrimp, Xiphopenaeus kroyeri (Heller)

2008 ◽  
Vol 50 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Flávia A. Gonçalves ◽  
Manoel Andrade Neto ◽  
José N. S. Bezerra ◽  
Andrew Macrae ◽  
Oscarina Viana de Sousa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antimicrobial Effect ◽  
Enteric Bacteria ◽  
Psidium Guajava ◽  
Salmonella Spp ◽  
Leaf Extracts ◽  
Xiphopenaeus Kroyeri ◽  
Guava Leaves ◽  
Guava Leaf

Guava leaf tea of Psidium guajava Linnaeus is commonly used as a medicine against gastroenteritis and child diarrhea by those who cannot afford or do not have access to antibiotics. This study screened the antimicrobial effect of essential oils and methanol, hexane, ethyl acetate extracts from guava leaves. The extracts were tested against diarrhea-causing bacteria: Staphylococcus aureus, Salmonella spp. and Escherichia coli. Strains that were screened included isolates from seabob shrimp, Xiphopenaeus kroyeri (Heller) and laboratory-type strains. Of the bacteria tested, Staphylococcus aureus strains were most inhibited by the extracts. The methanol extract showed greatest bacterial inhibition. No statistically significant differences were observed between the tested extract concentrations and their effect. The essential oil extract showed inhibitory activity against S. aureus and Salmonella spp. The strains isolated from the shrimp showed some resistance to commercially available antibiotics. These data support the use of guava leaf-made medicines in diarrhea cases where access to commercial antibiotics is restricted. In conclusion, guava leaf extracts and essential oil are very active against S. aureus, thus making up important potential sources of new antimicrobial compounds.

scholarly journals The Potential of Guava Leaf Extract as A Natural Preservation for Fish

2020 ◽  
Vol 2 (1) ◽  
pp. 62-69
Author(s):  
Riyanto
Keyword(s):  
Leaf Extract ◽  
Methanolic Extract ◽  
Psidium Guajava ◽  
Control Treatment ◽  
Fish Quality ◽  
High Protein Content ◽  
Fish Samples ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Good Medium

Fish is kinds of food that easy to spoil. High protein content in fish makes the food is a good medium for microorganisms. People commonly buy fish for consumption, however, not all fish are consumed at the same time. Fishes were stored in freezer for several days or preserved with additional chemical preservation. The use of organic preservatives have no harm. This paper discussed guava (Psidium guajava L) leave as natural fish preservation. The objectives were to analyze the potency of the leaves as a natural preservation for mackerel fish (Rastrelliger sp.). Fime parameters used to determine the fish quality as follows : gills, eye, texture, odour and mucilage. The treatments were leaf methanolic extract with doses 0, 20, 40, 60 and 80%. Data were taken on from 1 to 5 days after storage. The results showed that (i) Fish quality was decline and start to depraved at 2 day storage, especially at control treatment (0% extract). Treatment at doses 60 and 80%, the fish quality were still relatively good over 3 days storage. Even though the fish samples have been starting spoilage, those fishes were still available to be consumed. At the same time, with the other treatment, the fish samples have been spoiled and no more available to be consumed. However, at 5th day of storage, all samples at all treatments were spoiled. Overall results, it can be concluded that extract of guava leaves can be has as fish preservativedosage

scholarly journals Preliminary Phitochemical Analysis Of Guava Leaves (Psidium guajava L.) Extract As Antidiarrheal In Calves

2019 ◽  
Vol 3 (2) ◽  
pp. 21
Author(s):  
I Wayan Sudira ◽  
I Made Merdana ◽  
Suci Nur Qurani
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
The West ◽  
Active Compounds ◽  
Central Java ◽  
Guava Leaves ◽  
Guava Leaf

Guava plants (Psidium guajava L) are fruit plants originating from South America and can thrive in the territory of Indonesia. Now the biggest center for guava cultivation is spread in DKI Jakarta, West Java, Central Java, East Java, Yogyakarta, Bali, West Nusa Tenggara, Sumatra and Kalimantan. In addition to taking the fruit, guava leaves are also used as a traditional medicine for the treatment of diarrhea in human and animals. This study aims to identify active compounds in guava leaves. That is a secondary  metabolites with pharmacological effects as anti diarrhea such as flavonoid, alkaloid, tannins and essential oils. The leaves sample used in this study were taken from farmers in the West Denpasar area, Bali. Extraction of guava leaf leaves using 96% ethanol, and phytochemical analysis was carried out to detect the presence of active compounds. The data obtained is presented and analyzed descriptively qualitatively. The results showed that the ethanol extract of guava leaves contained active compound inclaude steroid/triterpenoid, phenolics, alkaloids, flavonoids, saponins and tannins. It can be concluded that guava leaf extract contains are potential antidiarrheal

scholarly journals UJI EFEK EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava L) TERHADAP KADAR KOLESTEROL TOTAL TIKUS WISTAR (Rattus norvegicus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Irianto Girik Allo ◽  
Pemsi Mona Wowor ◽  
Henoch Awaloei
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
Guava Leaves ◽  
Guava Leaf

Abstract: This research aims to determine the effect of ethanol extract of guava leaves on levels of total cholesterol of rat that is induced propylthiouracil. This was an experimental research. The sample used was 15 rats were divided into 5 groups (1 group is consist of 3 rats), namely: 1) positive control, 2) extract dose of 200 mg/kgBB, 3) extract dose of 400 mg/kgBB, 4) extract dose of 800 mg/kgBB and 5) negative control. Group 1 to 4 are given propylthiouracil given for 14 days, then given treatment appropiate with the group. The result show that: 1) the average of total cholesterol levels increased after administration propylthiouracil from 156.34 mg/dL to 247.84 mg/dL, 2) at a dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB showed a decline in total cholesterol levels by 32.73%, 58.73% and 47.26%, 3) the positive control decreased total cholesterol levels by 39.46% and 4) total cholesterol levels on negative is unstable (there are up and some are down). The result of this research concluse that: guava leaf extract at dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg kgBB have an effect in lowering total cholesterol of rat had been induced prophylthiouracil. Keywords: guava leaves, total cholesterol.     Abstrak: Penelitian ini bertujuan untuk mengetahui efek dari ekstrak etanol daun jambu biji terhadap kadar kolesterol total tikus wistar yang diinduksi propiltiourasil. Penelitian ini merupakan penelitian eksperimental. Sampel yang digunakan berjumlah 15 tikus wistar yang dibagi menjadi 5 kelompok (1 kelompok terdiri dari 3 tikus wistar), yaitu: 1) kontrol positif, 2) ekstrak dosis 200 mg/kgBB, 3) ekstrak dosis 400 mg/kgBB, 4) ekstrak dosis 800 mg/kgBB dan 5) kontrol negatif. Kelompok 1 sampai 4 diberikan propiltiourasil selama 14 hari, kemudian diberikan perlakuan sesuai kelompoknya. Hasil penelitian yang diperoleh: 1) rata-rata kadar kolesterol total meningkat setelah pemberian propiltiourasil yaitu dari 156,34 mg/dL menjadi 247,84 mg/dL, 2) pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB menunjukkan terjadi penurunan kadar kolesterol total sebesar 32,73%, 58,73% dan 47,26%, 3) pada kontrol positif terjadi penurunan kadar kolesterol total sebesar 39,46% dan 4) kadar kolesterol total pada kelompok negatif tidak stabil (ada yang naik dan ada yang turun). Hasil penelitian ini menyimpulkan bahwa ekstrak daun jambu biji pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB mempunyai efek dalam menurunkan kadar kolesterol total tikus wistar yang telah diinduksi propiltiourasil. Kata kunci: daun jambu biji, kadar kolesterol total.

scholarly journals PENGARUH EKSTRAK ETANOL DAUN JAMBU AIR (SYZYGIUM AQUEUM) TERHADAP BAKTERI ISOLAT KLINIS

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Titi Hariyati ◽  
Dwi Soelistya Dyah Jekti ◽  
Yayuk Andayani
Keyword(s):  
Ethanol Extract ◽  
Clinical Isolates ◽  
E Coli ◽  
Randomized Design ◽  
Inhibition Concentration ◽  
The Family ◽  
Ethanol Extracts ◽  
Guava Leaves ◽  
Guava Leaf

Syzygium aqueum plant of the family Myrtaceae is native of Malaysia and Indonesia and is known as the water rose. The active compound is useful as an antibacterial. This study aimed to determine the effects of ethanol extracts of guava leaves (S. aqueum) as an antimicrobial against clinical isolates bacteria in vitro. The design used in experiment was a Completely Randomized Design (CRD). Data were analyzed using the Kruskal-Wallis test. Results of statistical analysis using SPSS 20 for windows indicated that the ethanol extract of guava leaves has a significant (P<0,05) effect in inhibiting the growth of clinical isolates bacteria. MIC (Minimum Inhibition Concentration) of ethanol extracts for each bacterium can not be determined because the guava leaf extract was very dark. The MBC (Minimum Bacterisidal Concentration) of ethanol extract of guava leaf  for both S. aureus and S. dysenteriae was 20%.  The MBC of the extract for E. coli, S. thypi, V. cholerae was 25%, where as for B. cereus was at concentration of 50%. It can be concluded that guava leaf has highly potential as a source of antimicrobial agent.Keywords:Antibacteria, Guava Leaf, Clinical Isolates Bacteria, Ethanol Extract

scholarly journals A Study on Bioadsorption of Synthetic Dye Using Guava (Psidium guajava) Leaves

2020 ◽  
Vol 5 (9) ◽  
pp. 215-218
Author(s):  
Manasee Vichare ◽  
Prashant Masali
Keyword(s):  
Psidium Guajava ◽  
Water Bodies ◽  
Brilliant Blue ◽  
Tropical Region ◽  
Coomassie Brilliant Blue ◽  
Leaf Powder ◽  
Blue Solution ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Coomassie Brilliant

Guava (Psidium guajava, white Indian) is common fruit bearing plant found throughout the Indian peninsula. It is said to be native to Mexico and Southern America and now can be found in many of the Asian countries. It is grown in tropical and sub-tropical region around the world. Guava leaves have shown many properties like antibacterial, bioadsorbent, etc. The bioadsorbent property can be exploited to remove coloured residues from water bodies. Problems like water pollution have arisen due to rapid industrialization and urbanization. Various harmful dyes like Coomassie brilliant blue, used in both textile industries and laboratories, are expelled into water bodies. To reduce the effects of such dye on ecology the research was carried out using guava leaves as bioadsorbent. That being the case the Coomassie brilliant blue solution at different pH was treated with guava leaf powder and the decrease in absorbance was noted at 625 nm. The Guava leaves were obtained from Mumbai suburban. The leaves were dried and powdered for further testing as bioadsorbent. All the chemicals have been used were of analytical grade. The study showed high absorption rate in acidic medium as the colour changed from dark blue to light blue. The optimum pH for bio-adsorption was found to be 3. As the amount of guava leaf powder increased, the rate of adsorption also increased. Guava leaf powder is effective as a bioadsorption as rate of adsorption increases with increase in guava leaf powder. With further optimization it can become potential source of adsorbent.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli

2016 ◽  
Vol 16 (1) ◽  
pp. 51-56
Author(s):  
Hilda Maysarah ◽  
Rika Apriani ◽  
Misrahanum Misrahanum
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Guava Leaf ◽  
Mic Value

An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli.  MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.

scholarly journals The Chewable Tablet of Guava Leaves Extract (Psidium guajava L.) with Breadfruit Starch as Binder

2019 ◽  
Vol 4 (1) ◽  
pp. 8-16
Author(s):  
Pramulani Mulya Lestari ◽  
Septiana Tri Pamungkas
Keyword(s):  
Particle Size ◽  
Dental Caries ◽  
Particle Size Distribution ◽  
Water Immersion ◽  
Research Result ◽  
Psidium Guajava ◽  
Chewable Tablet ◽  
Starch Concentration ◽  
Chewable Tablets ◽  
Guava Leaves

Breadfruit starch can be used as a binder on the chewable tablet because it consists of amylose and amylopectin which can increase the cohesiveness and make a good bond with the powder. Guava leaves extract can kill the bacterial growth of Streptococcus mutans so it can be served to prevent dental caries. This research aimed to know whether increased concentrations of breadfruit starch can affect the physical characteristics of the chewable tablet of guava leaves extract. The chewable tablets were formulated into 4 formulas with different concentration of mucilago breadfruit starch, such as 10%, 13%, 16% and 19%. Breadfruit starch as a binder obtained from an almost ripe the breadfruit and then was crushed and soaked to occur separation between water immersion and amylum, and then amylum was dried and made as mucilago and the mucilago was added into the formula. The result of the characteristic of starch, the powder was true breadfruit starch. The flowability and particle size distribution of granule was evaluated. Then, the granule was made into the chewable tablet. The research result showed that the increase of mucilago breadfruit starch concentration increased the hardness and decreased friability of chewable tablet.

Biocontrol of Food Spoilage Microorganisms using Leaf extracts from Magnifera indica (Mango) and Psidium guajava (Guava).

2020 ◽  
Vol 45 (4) ◽  
Author(s):  
F.O. Oseghale ◽  
K.A. Fasina ◽  
A. Ohifueme ◽  
J. Omoruyi
Keyword(s):  
Psidium Guajava ◽  
Penicillium Oxalicum ◽  
Potassium Sorbate ◽  
Food Spoilage ◽  
Leaf Extracts ◽  
Benin City ◽  
Microbiological Techniques ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Spoilage Microorganisms

The shelf life of processed food is greatly reduced by spoilage microorganisms, which has resulted in great losses to food processors, wholesalers, retailers and even consumers. This study was aimed at controlling food spoilage microorganisms using leaf extracts from mango and guava. Fried meat (beef, chicken, grass-cutter, and goat meat), fried fish and fresh bread were purchased from Benin City, and left to spoil, after which spoilage organisms were isolated, identified and characterized using standard microbiological techniques. Mango and guava leaf extracts (ethanolic and aqueous), with Potassium sorbate and sodium nitrite was used. Significant highest (<0.05) (15.67±0.88 mg/ml) antibacterial activity of Mango leaf extract was recorded against Escherichia coli, and significant highest (<0.05) antifungal activity (19.67±0.33) was recorded against Penicillium oxalicum. Ethanolic leaf extracts of mango had better MICs (6.25 mg/ml) against E. coli tested than guava (25 mg/ml). In addition, ethanolic leaf extracts from Mango did not have any effect on Bacillus polymyxa. This study suggests that extracts from Mango and Guava leaves can be explored for controlling spoilage organisms; however, ethanolic extracts are more potent than aqueous extracts.

scholarly journals Pemanfaatan Ekstrak Daun Jambu Biji (Psidium guajava Linn) Sebagai Antibakteri dan Antifungi

2017 ◽  
Vol 6 (2) ◽  
pp. 41 ◽  
Author(s):  
Siti Nuryani
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Laboratory Test ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Inhibitory Power ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

Guava (Psidium guajava Linn) is found throughout Indonesia. The leaves contain tannins that can be used as antibacterial and antifungal. The aims of this research are to determine the antiseptic power of guava leaf as antifungal and antibacterial.This research is pre experiments research with laboratory test to determine the inhibitory power of guava ethanol extract as anti-bacterial and anti-fungal. Guava leaves are old made 70% ethanol extract in LPPT UGM using maceration method. The extract made 3 concentrations ie 25%, 50% and 75%. Each concentration was tested for inhibitory by knowing the diameter of growth barrier to Candida albicans and Staphylococcus aureus. Each concentration is repeated 5 times, resulting in 15 data. The data were analyzed descriptively to illustrate their potential comparisons with chlorhexidine as standard materials. The extract of guava leaf using ethanol 70% in laboratory test resulted in average inhibitory zone diameter as follows: for mushroom C.albicans with extract 25%, 50%, and 75% were 13.4mm, 17.6mm, and 19.4mm. While for S. aureus is 2.2mm, 25.6mm, and 27.2mm. The effect of antifungal power of guava leaf extract (Psidium guajava Linn.) on the growth of Candida albicans fungus is smaller compared to Staphylococcus aureus bacteria

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