Assessment of Degree of Association Between Markers of Oxidative Stress in Lead Poisoned Mice

2021 ◽  
Vol 15 (9) ◽  
pp. 2952-2954
Author(s):  
Roomisa Anis ◽  
Misbah-ul- Qamar ◽  
Ayesha Shafqat ◽  
Ayesha Aftab ◽  
Zarafshan Bader ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Negative Correlation ◽  
Pearson Correlation ◽  
Control Group ◽  
P Value ◽  
Standard Diet ◽  
Strong Negative Correlation ◽  
Study Results ◽  
Degree Of Association

Lead (Pb) is an abundant and one of the most lethal metals found in the earth’s crust. Its use by humans dates back to thousands of year. Even the low doses of lead are responsible for the production of reactive oxygen species which leads to oxidative load. This oxidative stress mitigates production of malondialdehyde (MDA) and down regulates antioxidant enzyme superoxide dismutase (SOD). Study Design: Quasi experimental Study. Place and duration of study: Department of Biochemistry, ANMCH, Islamabad, Pakistan in collaboration with NIH, Islamabad from November, 2018 to April, 2019. Methodology: A total of 40 BALB/c mice were divided into two groups of 20 mice each. Group  was given normal standard diet. Group  was given lead acetate in drinking water with normal diet without any supplementation. Levels of malondialdehyde were measured by using Thiobarbituric acid reactive substances (TBARS) and Superoxide Dismutase (SOD) was estimated by xanthine oxidase method at the end of study. Results: The results of our study showed increase in MDA and decrease in SOD in lead treated group when compared with the control group. Pearson correlation was applied to assess the degree of association between two parameters, it showed significant negative correlation with value of r = -0.96 and p-value of 0.001 Conclusion: It was concluded from our study that increase in MDA leads to decrease in SOD indicating strong negative correlation in lead poisoned mice. Key words: Lead poisoning, Malondialdehyde, Oxidative Stress, Superoxide Dismutase

scholarly journals Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Okky Irtanto ◽  
Alex Pangkahila ◽  
IGM Aman
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Mus Musculus ◽  
Liver Tissue ◽  
Day Treatment ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstract: Overtraining accelerates aging due to the excessive production of free radicals that can cause oxidative stress. Banana floret extract contains bioactive compounds with antioxidant capacity which can increase the body's defence to deal with the oxidative stress by increasing the level of superoxide dismutase (SOD). This study was aimed to prove that the banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in overtraining-induced mice (Mus musculus) BALB/c liver. This was a true experimental study with the post-test only control group design. Subjects were 36 male mice (Mus musculus), BALB/c strain, 12 weeks old, weighing 20-22 g, which were divided into two groups with 18 mice each. The control group (P0) was treated with a placebo of 1 ml aquadest and overtrained for 14 days meanwhile the treatment group (P1) was treated with banana (Musa x paradisiaca) floret extract of 400 mg/kgBW/day and overtrained for 14 days. The results showed that after 14-day treatment, the mean SOD level in the liver tissue of the P0 group was 568.82±9.558 U/mg protein whereas in the P1 group was 588.37±10.629 U/mg protein (P < 0.01). The t-independent test showed a t value of -5.804 and a P value of 0.000 which indicated that after treatment, the levels of SOD in liver tissue of both groups were significantly different. Conclusion: Banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in the liver tissue of overtraining-induced mice (Mus musculus) BALB/c.Keywords: banana floret, SOD, liver, overtrainingAbstrak: Aktivitas fisik berlebih mempercepat penuaan karena meningkatkan produksi radikal bebas yang dapat menyebabkan stres oksidatif. Ekstrak floret pisang mengandung senyawa bioaktif dengan kapasitas antioksidan yang dapat meningkatkan pertahanan tubuh dalam menghadapi stres oksidatif melalui peningkatan kadar superoksida dismutase (SOD). Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 36 ekor mencit (Mus Musculus) BALB/c, jantan, berumur 12 minggu, berat badan 20-22 gr, yang dibagi menjadi dua kelompok masing-masing berjumlah 18 ekor mencit. Kelompok kontrol (P0) diberikan plasebo berupa aquadest sebanyak 1 ml dengan aktivitas fisik berlebih selama 14 hari, dan kelompok perlakuan (P1) diberikan ekstrak floret pisang raja (Musa x paradisiaca) dosis 400 mg/kgBB mencit per hari dicampur aquadest hingga 1 ml dengan aktivitas fisik berlebih selama 14 hari. Hasil penelitian menunjukkan rerata kadar SOD jaringan hati pada kelompok kontrol (P0) sesudah perlakuan (post-test) ialah 568,82±9,558 U/mg protein, sedangkan pada kelompok perlakuan (P1) ialah 588,37± 10,629 U/mg protein. Analisis kemaknaan dengan T-Independent mendapatkan nilai t= -5,804 dan nilai P = 0,000 yang menunjukkan bahwa sesudah perlakuan (post-test), kadar SOD jaringan hati pada kedua kelompok berbeda sangat bermakna. Simpulan: Ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih.Kata kunci: floret pisang raja, SOD, hati, aktivitas fisik berlebih

Penatalaksanaan IMD pada Ibu Postpartum Sectio Caesarea Mempengaruhi Status Gizi dan Kecepatan Produksi ASI

2020 ◽  
Vol 2 (2) ◽  
pp. 112-120
Author(s):  
Nursari Abdul Syukur ◽  
Susi Purwanti
Keyword(s):  
Breast Milk ◽  
Nutritional Status ◽  
Milk Production ◽  
Quantitative Research ◽  
Milk Protein ◽  
Sectio Caesarea ◽  
Control Group ◽  
P Value ◽  
Study Results

Many mothers who give birth to Sectio Caesarea (SC) do not Initiate Early Breastfeeding (IMD), which fails exclusive breastfeeding. This study aimed to determine the effect of IMD management in postpartum SC mothers on nutritional status, speed of milk production, and quality of breast milk protein. Method: quantitative research with quasi approach experiment. The research design used was a pre-post-test control non-equivalent control group. A sampling of this study used the Consecutive method sampling with a sample of 20 mothers who gave birth by cesarean section (SC). Hypothesis testing uses the independent t-test and the Mann-Whitney test. The study results showed an influence on the management of IMD in postpartum SC mothers on the speed of ASI production (p-value=0.004) and nutritional status (p-value=0.028). There was no effect of IMD management on postpartum SC mothers on the quality of breast milk protein (p-value = 0.543). This study recommends that the hospital implement an IMD promotion program before the abdominal wall is closed as a form of intervention to increase milk production and maternal nutritional status

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Alteration in the oxidative status of Drosophila melanogaster Meigen (Diptera: Drosophilidae) fed with a diet containing Centaurea depressa M. Bieb. (Asteraceae)

2020 ◽  
Vol 70 (2) ◽  
pp. 227-237
Author(s):  
Eda Güneş
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Total Protein ◽  
Protein Carbonyl ◽  
Control Group ◽  
Carbonyl Content ◽  
Protein Carbonyl Content ◽  
Freeze Dried ◽  
Dried Extract

Abstract The aim of the this study was to evaluate the effects of fresh, dried and freeze-dried Centaurea depressa M. Bieb. (Asteraceae) on the oxidant and antioxidant status of the model organism D. melanogaster Meigen (Diptera: Drosophilidae) experimentally. The study was carried out from 2016 to 2019, and plant leaf extracts (0-50 mg/l) were added to insect standard artificial diets. The total protein, protein carbonyl content and glutathione-S-transferase, superoxide dismutase and catalase activities were quantified at the insect’s third larval stage. Our data showed that protein carbonyl content varied from 2.70 nmol/mg protein in the control group to 59.11 nmol/mg protein in the group fed with fresh leaf extract signifying induction of oxidative stress. All extracts increased the levels of all antioxidant enzymes and decreased the amounts of total protein. Meanwhile, the group fed with the freeze-dried extract showed no significant difference in the levels of total protein and protein carbonyl content except at the 50 mg/l concentration of the extract. Moreover, this group had superoxide dismutase and catalase activities 4 to 5 times higher than in the control group. In conclusion, induction of oxidative stress indicates that the fresh form of C. depressa leaves may have potential as a natural pesticide, whereas induction of endogenous antioxidant enzymes by the freeze-dried extract suggest its potential as an antioxidant.

scholarly journals In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

2013 ◽  
Vol 64 (4) ◽  
pp. 553-559 ◽  
Author(s):  
Seyed Fazel Nabavi ◽  
Solomon Habtemariam ◽  
Antoni Sureda ◽  
Akbar Hajizadeh Moghaddam ◽  
Maria Daglia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin C ◽  
Gallic Acid ◽  
Sodium Fluoride ◽  
Enzyme Activities ◽  
Control Group ◽  
Rat Erythrocytes ◽  
Pre Treatment

Abstract Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m-3 for one week). Gallic acid at 10 mg kg-1 and 20 mg kg-1 and vitamin C for positive controls (10 mg kg-1) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.

Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study

CNS Spectrums ◽  
2017 ◽  
Vol 24 (03) ◽  
pp. 333-337 ◽  
Author(s):  
Maiara Zeni-Graiff ◽  
Adiel C. Rios ◽  
Pawan K. Maurya ◽  
Lucas B. Rizzo ◽  
Sumit Sethi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
At Risk ◽  
Superoxide Dismutase ◽  
High Risk ◽  
Glutathione Peroxidase ◽  
Healthy Control Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Ultra High Risk ◽  
Healthy Control

IntroductionOxidative stress has been documented in chronic schizophrenia and in the first episode of psychosis, but there are very little data on oxidative stress prior to the disease onset.ObjectiveThis work aimed to compare serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in young individuals at ultra-high risk (UHR) of developing psychosis with a comparison healthy control group (HC).MethodsThirteen UHR subjects and 29 age- and sex-matched healthy controls (HC) were enrolled in this study. Clinical assessment included the Comprehensive Assessment of At-Risk Mental States (CAARMS), the Semi-Structured Clinical Interview for DSM-IV Axis-I (SCID-I) or the Kiddie-SADS-Present and Lifetime Version (K-SADS-PL), and the Global Assessment of Functioning (GAF) scale. Activities of SOD and GPx were measured in serum by the spectrophotometric method using enzyme-linked immunosorbent assay kits.ResultsAfter adjusting for age and years of education, there was a significant lower activity of SOD and lower GPX activity in the UHR group compared to the healthy control group (rate ratio [RR]=0.330, 95% CI 0.187; 0.584, p&lt;0.001 and RR=0.509, 95% CI 0.323; 0.803, p=0.004, respectively). There were also positive correlations between GAF functioning scores and GPx and SOD activities.ConclusionOur results suggest that oxidative imbalances could be present prior to the onset of full-blown psychosis, including in at-risk stages. Future studies should replicate and expand these results.

scholarly journals The Influence of Practicum-Based Outdoor Inquiry Model on Science Process Skills in Learning Physics

2019 ◽  
Vol 8 (1) ◽  
pp. 23-33
Author(s):  
Yeni Ristya Wardani ◽  
Mundilarto Mundilarto ◽  
Jumadi Jumadi ◽  
Insih Wilujeng ◽  
Heru Kuswanto ◽  
...  
Keyword(s):  
Sampling Technique ◽  
Control Group ◽  
P Value ◽  
Cluster Sampling ◽  
Process Skills ◽  
Science Process Skills ◽  
Study Results ◽  
Post Test ◽  
Essay Test ◽  
Test Control

The main purpose of this study is to find out the influence of practicum-based outdoor inquiry model on science process skills. Quasi-experiment design was employed as the research method by Matching Only Post-test Control Group. The research sample was taken using a cluster sampling technique. The samples consisted of experimental class (XI MIA 1) in a total of 25 students treated with practicum-based outdoor inquiry model. The control class (XI MIA 3) consisted of 29 students treated with direct instruction model. In collecting the data, essay test was used in the form of worksheets containing the aspect of science process skills of formulating hypotheses, designing experiment, interpreting data into tables, and drawing a conclusion (inferring). The study results demonstrate that practicum-based outdoor inquiry model was effective in improving the science process skills. It was shown in p-value (significant) of 0.00. The implication of this study is the need to design a practicum-based outdoor inquiry model by noticing what skills are going to improve by means of more varied outdoor activities

scholarly journals A comparative study between vacuum assisted wound closure vs conventional dressing in non-healing ulcers

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2214-2223
Author(s):  
Ajay Rex R ◽  
Balaji D ◽  
Lakshmana R ◽  
Gopi Ramu ◽  
Reka
Keyword(s):  
Hospital Stay ◽  
Wound Closure ◽  
Healing Process ◽  
Control Group ◽  
P Value ◽  
Baseline Characteristic ◽  
Age Group ◽  
Average Hospital ◽  
Study Results ◽  
Study Population

In a surgical ward, acute and continual wounds have an effect on a minimal of 1% of the population. Vacuum-assisted wound closure (VAC) is a technique of Negative pressure in the wound to improve the healing process. To study the advantage of a vacuum assisted closure over conventional dressing in the management of chronic non-healing diabetic ulcers. To study the difference in the rate of amputation, hospital stays in case and control groups. Group1-case group – vacuum associated closure therapy. Group 2-Control group -conventional dressings. Most of the patients in the study population was in the age group of 41 -60 years. 82% of the study population was within the age group of 41-60 years. The two groups are comparable with their baseline characteristic of age, and the P-value is less than 0.05. Wounds were more common in males than females. Out of the 44 patients, 26 were male, i.e. 57% of the study population were males. About 68% of wounds occurred in the foot. About 50% of the culture showed staphylococcus. Nearly 27% of study participants had no growth. The hospital stay is less in VAC dressing when compared to the conventional dressings, who have an average hospital stay of 28 days and the relation is statistically significant (p-value<0.05). Mean hospital stay in Vacuum is 21 compared to stay of 28 in conventional dressings group. Patients in Vacuum had 12 SSG,9 discharge and 1 amputation. There is no statistically significant association in terms of grade of ulcer between the two groups(P =0.23). There is a statistically significant association between VAC and conventional in terms of the results of the Doppler study. (P<0.01). From the study results, it is obvious that VAC dressing has many advantages in terms of Low no of amputation, Earlier discharge, Minimal infection, Lesser complications, Healing in a better way.

scholarly journals Peningkatan Kemampuan Ketrampilan Sosial Pasien Gangguan Jiwa di Masyarakat dengan Terapi Hortikultura

2020 ◽  
Vol 4 (1) ◽  
pp. 77-82
Author(s):  
Widyo Subagyo ◽  
Dyah Wahyuningsih ◽  
Mukhadiono Mukhadiono
Keyword(s):  
Social Skills ◽  
Mental Disorders ◽  
Quantitative Research ◽  
Intervention Group ◽  
Control Group ◽  
P Value ◽  
Horticultural Therapy ◽  
The Social ◽  
Study Results ◽  
Significant Difference

This study aimed to determine the effect of the development of horticultural therapy to improve the social skills of mental disorders patients in the community. This research's method is quantitative research with a pre-posttest queasy experimental research design with a control group. The study results the social skills score before and after treatment in the intervention group given horticultural therapy was marked with a p-value of 0.01 (<0.05). In conclusion, there is a significant difference in the mean difference in social skills scores between the intervention and control groups.   Keywords: Mental Disorders, Social Skills, Horticultural Therapy

scholarly journals Andra’s Nursing Informatic System Application (Annisa) dalam Upaya Meningkatkan Pengetahuan Perawat tentang Dokumentasi Keperawatan

2020 ◽  
Vol 4 (1) ◽  
pp. 20-30
Author(s):  
Candra Saputra ◽  
Yulastri Arif ◽  
Fitra Yeni
Keyword(s):  
Quantitative Research ◽  
Intervention Group ◽  
Control Group ◽  
P Value ◽  
Nursing Documentation ◽  
Control Group Design ◽  
Study Results ◽  
Quasi Experimental ◽  
Time Series Approach ◽  
Nurses Knowledge

This study aimed to determine the effectiveness of ANNISA development on nurses' knowledge of nursing documentation. This method uses quantitative research with a quasi-experimental design and a time series approach non-equivocal control group design. The study results were there were differences in the measurement of nurses' knowledge about nursing documentation in the intervention group before the intervention by measuring posttest-1 with p-value = 0.000. There were differences in nurses' experience about nursing documentation between the intervention group and the control group in the posttest-1 and posttest-2 measurements after the intervention using ANNISA with p-value = 0,000. There was an increase in differences in nurses' knowledge about nursing documentation from the pretest, posttest-1, and posttest-2 measurements after using ANNISA in the intervention group and the control group with p-value = 0.000. In conclusion, ANNISA development affects increasing the knowledge of nurses about nursing documentation   Keywords: ANNISA, Nursing Documentation, Knowledge

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