scholarly journals Effect of Nitrogen Sources on Microbial Biomass Nitrogen under Different Soil Types

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Haytham M. El-Sharkawi

Knowledge to increase the microbial biomass nitrogen (MBN) as a bulk of free-living microbes in paddy soil is limited. The potential benefit of these microorganisms was evaluated, in this study, under different nitrogen sources and two paddy soils. The results revealed that pots treated with organic matter recorded the maximum value of the total N uptake and MBN, followed by the Urea treated pots. Pots amended with sludge exhibited a higher microbial N forming ability than those amended with straw compost under both soils. But ammonium concentration in soil increased with straw compost application. Under fresh soil treatment, microbial N uptake rate and proportion of plant nitrogen derived from microbial nitrogen sources () were higher than autoclaved soil. A positive correlation was found between the and the total N in rice shoot in both soils. Finally, we can say that MBN was governed not only by the soil nitrogen content but also by the type of the nitrogen source. The addition of sludge to fresh soil increased total MBN and consequently could be indirectly beneficial to rice production especially in poor soils. Thus, soil microbes contribute to plant growth by serving the available nitrogen during the season.

HortScience ◽  
2009 ◽  
Vol 44 (2) ◽  
pp. 354-361 ◽  
Author(s):  
André Snyder ◽  
Matthew J. Morra ◽  
Jodi Johnson-Maynard ◽  
Donald C. Thill

Brassicaceae seed meals (BSMs) average 6% nitrogen (N) by weight and contain glucosinolates (GLSs) that produce biologically active compounds. A two-season field study was initiated to determine how Brassica juncea L., Brassica napus L., and Sinapis alba L. seed meals, each with different glucosinolate profiles, alter carrot (Daucus carota L. subsp. sativus) growth, microbial biomass N (MBN), and soil N mineralization. BSM applications of 1 and 2 t·ha−1 36 days before planting did not influence carrot emergence, whereas carrot emergence decreased up to 40% in S. alba treatments seeded 15 days after BSM application. Crop quality was unaffected by BSM treatments and total fresh market yields were equal to or higher than the unamended controls in both years. At 4 and 8 days after seed meal application, MBN in the high-GLS B. juncea and S. alba treatments was 48% to 67% lower than in the low-GLS B. napus treatment. Seasonal apparent net N mineralized expressed as a percentage of the total N applied in the seed meals was unaffected by glucosinolate concentration and ranged from 30% to 81% across both years. BSMs can be used to increase soil inorganic N and carrot yields, but crop phytotoxicity is possible depending on the meal and its respective glucosinolate content. GLS degradation products inhibit microbial N uptake in the short term, but longer-term N availability is not compromised.


1973 ◽  
Vol 9 (2) ◽  
pp. 113-120 ◽  
Author(s):  
M. J. Jones

SUMMARYMaize was grown for three years at three levels of nitrogen, 56, 112 and 224 kg. N ha.−1, involving altogether nine different timing and splitting treatments. Measurements were made of grain yield, plant nitrogen status and total-N-uptake, and, in one year, movement of nitrate-N in control plot soils. Where only 56 kg. N ha.−1was applied, its time of application made very little difference to yield; at higher rates of nitrogen an unsplit application as late as seven weeks was very inefficient, but only at the highest rate did a split application give any appreciable yield increase over an unsplit application to the seed bed. Consideration of the soil nitrate-N data and the long-term pattern of rainfall distribution leads to the conclusion that leaching is unlikely to be a serious problem in the nitrogen nutrition of early-planted maize.


2014 ◽  
Vol 2014 (1) ◽  
pp. 1113-1125
Author(s):  
Xiaolong Geng ◽  
Michel C. Boufadel

ABSTRACT In April 2010, the explosion of the Deepwater Horizon (DWH) drilling platform led to the release of nearly 4.9 million barrels of crude oil into the Gulf of Mexico. The oil was brought to the supratidal zone of beaches (landward of the high tide line) by waves during storms, and was buried during subsequent storms. The objective of this paper is to investigate the biodegradation of subsurface oil in a tidally influenced sand beach located at Bon Secour National Wildlife Refuge and polluted by the DWH oil spill. Two transects were installed perpendicular to the shoreline within the supratidal zone of the beach. One transect had four galvanized steel piezometer wells to measure the water level. The other transect had four stainless steel multiport sampling wells that were used to collect pore water samples below the beach surface. The samples were analyzed for dissolved oxygen (DO), nitrogen, and redox conditions. Sediment samples were also collected at different depths to measure residual oil concentrations and microbial biomass. As the biodegradation of hydrocarbons was of interest, a biological model based on Monod kinetics was developed and coupled to the transport model MARUN, which is a two dimensional (vertical slice) finite element model for water flow and solute transport in tidally influenced beaches. The resulting coupled model, BIOMARUN, was used to simulate the biodegradation of total n-alkanes and polycyclic aromatic hydrocarbons (PAHs) trapped as residual oil in the unsaturated zone. Model parameter estimates were constrained by published Monod kinetics parameters. The field measurements, such as the concentrations of the oil, microbial biomass, nitrogen, and DO, were used as inputs for the simulations. The biodegradation of alkanes and PAHs was predicted in the simulation, and sensitivity analyses were conducted to assess the effect of the model parameters on the modeling results. Simulation results indicated that n-alkanes and PAHs would be biodegraded by 80% after 2 ± 0.5 years and 3.5 ± 0.5 years, respectively.


2019 ◽  
Vol 5 (02) ◽  
pp. 242-248
Author(s):  
V. Namdeo ◽  
N. G. Mitra ◽  
S. R. Jakhar ◽  
R. K. Sahu

Field experiments were conducted to Influence of different levels of nitrogen and Azospirillum inoculation on direct-seeded rice in a Vertisol, during kharif season of 2015 at Department of Soil Science and Agricultural Chemistry, JNKVV, Jabalpur. The experiment was laid out under randomized block design (RBD) with 3 replications and 8 treatments namely (unfertilized+uninoculated (UFUI), recommended dose of nitrogen 50%+uninoculated (RDN50%+UI), RDN75%+UI, RDN100%+UI, UF+Azospirillum (UF+Azosp.,), RDN50%+Azosp., RDN75%+Azosp and RDN100%+Azosp.,). It was observed that significant improvement was noticed in yield attributes and soil properties. The response from the treatment of RDN100% +Azosp., was found statistically best to increase available nitrogen (N) content in soil at 45 DAS and at harvest of the crop by 29% and 27%, respectively and N content in the plant, grain and straw by 46%, 50% and 55%, respectively over the control of UFUI. Similarly, trend was significantly enhanced total N uptake by crop with 129% over the control of UFUI. While, same treatment combination increasing azospiral population in rhizospheric soil at 45 DAS, 65 DAS and at harvest by 2.28, 2.07 and 2.05 log folds, respectively over the control of UFUI and enhanced yield attributes and yields of grain and straw of rice with 113 and 58%, respectively over the control of UFUI. While the treatment RDN100%+Azosp., exhibited numerically higher values but was statistically at par to RDN75%+Azosp.


2018 ◽  
Vol 13 (3) ◽  
pp. 651-662 ◽  
Author(s):  
Hugo Berthelot ◽  
Solange Duhamel ◽  
Stéphane L’Helguen ◽  
Jean-Francois Maguer ◽  
Seaver Wang ◽  
...  

AbstractNitrogen (N) is a limiting nutrient in vast regions of the world’s oceans, yet the sources of N available to various phytoplankton groups remain poorly understood. In this study, we investigated inorganic carbon (C) fixation rates and nitrate (NO3−), ammonium (NH4+) and urea uptake rates at the single cell level in photosynthetic pico-eukaryotes (PPE) and the cyanobacteria Prochlorococcus and Synechococcus. To that end, we used dual 15N and 13C-labeled incubation assays coupled to flow cytometry cell sorting and nanoSIMS analysis on samples collected in the North Pacific Subtropical Gyre (NPSG) and in the California Current System (CCS). Based on these analyses, we found that photosynthetic growth rates (based on C fixation) of PPE were higher in the CCS than in the NSPG, while the opposite was observed for Prochlorococcus. Reduced forms of N (NH4+ and urea) accounted for the majority of N acquisition for all the groups studied. NO3− represented a reduced fraction of total N uptake in all groups but was higher in PPE (17.4 ± 11.2% on average) than in Prochlorococcus and Synechococcus (4.5 ± 6.5 and 2.9 ± 2.1% on average, respectively). This may in part explain the contrasting biogeography of these picoplankton groups. Moreover, single cell analyses reveal that cell-to-cell heterogeneity within picoplankton groups was significantly greater for NO3− uptake than for C fixation and NH4+ uptake. We hypothesize that cellular heterogeneity in NO3− uptake within groups facilitates adaptation to the fluctuating availability of NO3− in the environment.


2011 ◽  
Vol 149 (4) ◽  
pp. 497-505 ◽  
Author(s):  
W. KAIYONG ◽  
F. HUA ◽  
T. RANAB ◽  
M. A. HANJRAC ◽  
D. BO ◽  
...  

SUMMARYCotton is the dominant crop in the northern Xinjiang oasis of China; it accounts for 0·78 of the total planting area and represents a major contribution to economic development. The objective of the present study is to determine how cotton plantation age affected chemical and microbiological properties of the soil. The time substitution method was used on plantation farmlands, reclaimed from uncultivated land 0, 5, 10, 15 and 20 years ago. A total of 250 soil samples, at depths of 0–200, 200–400, 400–600, 600–800 and 800–1000 mm, were collected from cotton fields in 10 farms of each age category. There were significant differences in soil organic carbon (SOC), total soil nitrogen (TSN), soil available nitrogen (SAN), soil microbial biomass carbon (SMBC) and soil microbial biomass nitrogen (SMBN). There were also differences in the activities of cellulase, invertase and urease between soil layers and plantation ages, and these were most evident in the 200–400 mm layer. The cumulative rates of SOC and SMBC in the 0–1000 mm soil layer at the 5-, 10-, 15- and 20-year sites were 0·89, 0·99, 1·01 and 0·92 mg/kg/yr and 16, 16, 16 and 15 mg/kg/yr, respectively, compared to that at the control site (0 year). The cumulative amounts of SOC and SMBC increased gradually and then decreased, reaching a maximum at plantation ages of 13·1 years and 11·1 years, respectively. This suggests that incorporation of post-harvest cotton residues could be used as an effective measure to improve SOC in farmland of Xinjiang Oasis, and may be recommended for adoption in cotton growing in semi-arid oasis agriculture.


Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 381
Author(s):  
Lili Guo ◽  
Huiwen Yu ◽  
Wenquan Niu ◽  
Mourad Kharbach

Nitrogen (N) transformation in soil directly determines the effectiveness of N for plant growth. Biochar has received evermore attention because of its significant ability to improve soil. However, the effects of biochar on N-related microorganisms (Lycopersicon esculentum Mill.) in tomato cultivation soil, N transformation, utilisation of water and N fertiliser, and tomato yield remain unclear. The objective of this study was to investigate the responses of N-related microorganisms to biochar and N fertilisation in soil, along with the implications of biochar for altering N transformation, N uptake by tomatoes, and utilisation of water and N fertiliser. A two-year greenhouse experiment containing six biochar levels under drip irrigation (0, 10, 30, 50, 70, and 90 t ha−1) and two N fertiliser application rates (190 and 250 kg ha−1) was conducted in the northwest of China. The results showed that adding biochar significantly promoted urease activity, microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and the number of amoA-type nitrifiers in the soil. The MBC:N ratio and the number of nirS-type denitrifiers were significantly inhibited when the added amount of biochar was greater than or equal to 30 t ha−1. Moreover, biochar can increase the water content in the soil and can reduce the N lost to leaching. The inorganic N (NO3− and NH4+) in the soil could be better maintained in the rootzone and better absorbed by tomato plants when adding 30, 50, and 70 t ha−1 of biochar. The amount of N fertiliser could be reduced by 24% without a significant loss of tomato yield when the amount of biochar added was over 30 t ha−1. It was indicated that the yield of tomatoes and the net profits were quadratically related to the application rate of biochar. In the test area, 53 t ha−1 of biochar with 190 kg ha−1 of N and 44.6 t ha−1 of biochar with 190 kg ha−1 of N were calculated to be the best amounts from the perspectives of tomato yield and net profit, respectively. Thus, biochar promotes N transformation by regulating N-related microorganisms; hence, it increases the inorganic N in the roots of the plants, reduces N lost to leaching, and significantly promotes the N absorption of tomatoes. The results in this research are of great significance for the development of management strategies for tomato maintenance, environmental protection, and resource conservation.


Atmosphere ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1166
Author(s):  
Yan Gao ◽  
Aizhen Liang ◽  
Yan Zhang ◽  
Neil McLaughlin ◽  
Shixiu Zhang ◽  
...  

Returning residue to soils is not only an effective nutrient management method, but also can reduce the air pollution caused by residue burning, which has become an important factor in global warming. However, it is not clear whether returning residue to the soil can affect the nitrogen mineralization and the nitrogen cycle process, and the environmental impact caused by the nitrogen loss in gaseous forms. Therefore, a pot experiment was conducted to study the effects of residue placement on the nitrogen turnover process, including microbial biomass N (MBN) and C (MBC), inorganic N, crop N uptake, and the contribution of residue-derived N to maize at different maize growth stages. Three treatments were assessed: no residue addition (T0), residue addition to the soil surface (T1), and residue incorporation into the 0–10 cm soil layer (T2). Soil samples were taken at the 0–5 and 5–10 cm layers for all residue treatments. Residue retention (T1 and T2) significantly affected the MBC and MBN contents and decreased MBC/MBN ratio at different maize growth stages. MBC/MBN markedly increased at the R1 stage compared to other growth stages. The differences in total inorganic nitrogen (TIN) were attributed to the balance in net N immobilization and net mineralization in the different maize growth stages. In addition, T2 significantly increased the residue-derived N source for maize by 11.3% compared to T0 in the R3 growth stage. Overall, relative to T1, T2 is a better agriculture management measure to promote N transformation and supply, and enhance residue-derived N release and uptake in maize.


2000 ◽  
Vol 80 (3) ◽  
pp. 483-488 ◽  
Author(s):  
Y. K. Soon ◽  
W. A. Rice ◽  
M. A. Arshad ◽  
P. Mills

Pipeline construction on the Grey soils of the Canadian boreal plains, which have a thin Ah horizon, could have considerable impact on their properties and productivity. This study was conducted because the effects of pipeline installation on crop yield and biological properties of these soils have not been well-documented. Soil was sampled from a Grey Luvisol and a Dark Grey Solod prior to pipeline construction in 1991, and in each of the following 3 yr. The right-of-way (RoW) was divided into three zones: a road (or work) area used for vehicular traffic; a trench where the pipeline was buried; and a pile (or spoil) area where soil was stockpiled during construction. The RoW was cropped to barley (Hordeum vulgare L.) in 1992 through 1994. Barley yield was low in 1992 (830–1120 kg ha−1), and near average (2050–3290 kg ha−1) in 1993 and 1994. Except for low shoot P concentration (1.1–1.3 mg g−1) in 1992, macronutrient concentrations (N, P and K) in barley tissues were within normal ranges. Soil organic carbon was reduced by 12–28% in all RoW areas in 1993 and 1994. Soil total N was reduced by 29–49% in all RoWs in 1992 and increased slightly from those levels in 1993 and 1994. Pipeline construction affected soil microbial biomass carbon (MBC) in the three RoW areas differently, and the effect was not consistent from year to year. However, the average level of MBC was not adversely impacted. In 1994, soil phosphatase activity in the RoW zones tended to be lower as compared to pre-pipeline installation, particularly in the pile area of the Dark Grey Solod. It is concluded that although some soil biological properties were degraded by pipeline construction, and barley yield was reduced in 1992, crop production in the following 2 yr was not significantly affected. Key words: Barley, carbon, microbial biomass, nitrogen, phosphatase, pipeline


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