scholarly journals Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations

2021 ◽  
Vol 42 (5) ◽  
pp. 2993-3006
Author(s):  
Weliton Vilhalba da Silva ◽  
◽  
Henrique Momo Ziemniczak ◽  
Flávia Barbieri Bacha ◽  
Rudã Brandão Santos Fernandes ◽  
...  
Keyword(s):  
Chronic Exposure ◽  
Lamellar Structure ◽  
Carassius Auratus ◽  
Blood Parameters ◽  
Pathological Changes ◽  
Blood Samples ◽  
Gill Histopathology ◽  
Interlamellar Space ◽  
Gill Morphology ◽  
Ionic Imbalance

The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-1) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-1 NaCl was 4 h, and 5 min at a concentration of 25 g L-1. The mortality of fish with 15 g L-1 NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-1 of NaCl. At concentrations of 20 and 25 g L-1, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-1 of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-1 of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.

scholarly journals Reviewing the Effect of 10 Days of Intense Exercise Period on Certain Blood Parameters of Tennis Players

2018 ◽  
Vol 6 (11) ◽  
pp. 95
Author(s):  
Muzaffer Selcuk ◽  
Vedat Cinar ◽  
Mucahit Sarikaya ◽  
Salih Oner
Keyword(s):  
Statistical Significance ◽  
Exercise Program ◽  
Blood Parameters ◽  
Intense Exercise ◽  
Negative Effects ◽  
Blood Samples ◽  
Tennis Players ◽  
Before And After ◽  
Exercise Period ◽  
Competition Period

This study aims to observe the possible negative effects that might occur on bio-chemistry and hemogram values of tennis players during the intense competition period by comparing the blood values of pre-competition period with 10 days of intense exercise. Blood samples were obtained from tennis team players who do not have any specific health problems and who study in university and regularly exercise. Mean age of the athletes are 22,40 ± 3,20 years and mean height is 179,83 ± 7,57 cm. This study is performed with 14 volunteer tennis players. Blood samples are obtained during the first day of the intense exercise program after the exercise, last day of the exercises and also right after the exercises. As per the obtained data, descriptive statistics are run (mean and standard deviation) and in order to compare the values of before and after the 10 days of intense exercise, Wilcoxon two related sample test was used. As per the results of the blood tests from before and after the exercise period, it is seen that values such as AST, ALT, MCH, MCHC and CK showed statistical significance (P<0.05). The athletes who prepared for the competitions with these values showed positive increases in bio-chemistry and hemogram values.

scholarly journals Evidence of Regeneration of Testicular and Epididymal Tissue Structure and Function Following Withdrawal from Sub-chronic Khat Exposure: Studies in the Rabbit Animal Model

2020 ◽  
pp. 10-23
Author(s):  
Albert W. Nyongesaa ◽  
Esther M. Malukib ◽  
Jemimah A. Simbaunib
Keyword(s):  
Body Weight ◽  
Chronic Exposure ◽  
Structure And Function ◽  
The Body ◽  
Endocrine Function ◽  
Eastern Africa ◽  
Blood Samples ◽  
Middle East Countries ◽  
Group I ◽  
And Function

Khat, Catha edulis, use is rampant in Eastern Africa and Middle East countries with associated reports of reproductive function impairment in the body of the user. Reports on recovery post long-term khat exposure are obscure. The present study investigated evidence of restoration of testicular and epididymal structure and function during withdrawal from cytotoxic damage caused by sub-chronic exposure of khat extract. Twenty-eight male rabbits were divided into 7 groups of 4 rabbits each. Group I (control) was administered normal saline while groups II, III and IV were administered 1.0 g/kg, 10 g/kg and 20 g/kg body weight of khat extract, respectively, via oral gavage on alternate days of the week for 12 weeks. Blood samples from animals were collected for hormonal assays followed by euthanasia using 26.4 mg/kg body weight of Sagatal sodium intramuscularly for testicular and epididymal histology. Group V, VI and VII were administered 1.0 g/kg, 10 g/kg and 20 g/kg body weight of khat extract, respectively, orally on alternate days of the week for 12 weeks followed by 1-month withdrawal period, blood samples collected for hormone assays and animals sacrificed for testicular and epididymal histology. High khat dose, 20 g/kg body weight, at sub-chronic exposure caused degeneration in spermatogenic cells with accompanying decrease in plasma FSH and testosterone. Histological output of Sertoli cells, Leydig cells and epididymal epithelium appeared unaffected in treatment groups. Post withdrawal data showed apparent regeneration of seminiferous epithelium and restoration of plasma FSH and testosterone comparable to control. It appears khat extract preferentially affected germ cell spermatogonia and subsequent daughter cells while stem cell spermatogonia were unaffected and contributed to regeneration of germinal epithelium and endocrine function.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

Detection of a novel haemoplasma species in fattening pigs with skin alterations, fever and anaemia

2020 ◽  
Vol 187 (2) ◽  
pp. 66-66 ◽  
Author(s):  
Julia Stadler ◽  
Julia Ade ◽  
Mathias Ritzmann ◽  
Katharina Hoelzle ◽  
Ludwig E Hoelzle
Keyword(s):  
Phylogenetic Analyses ◽  
Clinical Signs ◽  
Blood Parameters ◽  
Blood Samples ◽  
Management Procedures ◽  
Spleen Sample ◽  
Normocytic Normochromic Anaemia ◽  
Inflammatory Drugs ◽  
On Farm ◽  
Fattening Pigs

BackgroundIn a fattening farm in Southern Germany, skin alterations (urticaria, haemorrhagic diathesis) and high fever were observed in 30% of the pigs 2 weeks after arrival. Feed intake was severely compromised in affected pigs.MethodsAfter detailed clinical observation, blood samples from affected pigs were collected for haematological, PCR and serological investigations. In addition, pathological investigations were performed on one pig.Results and conclusionAnalysis of blood parameters revealed a normocytic, normochromic anaemia. A novel porcine haemoplasma species was detected in blood samples of affected pigs and spleen sample of the necropsied pig by PCR. Phylogenetic analyses based on the 16S rDNA showed 99% identity to a novel porcine haemoplasma (‘Candidatus (Ca.) M. haemosuis’) species which has recently been described in China. Interestingly, this is the first report of ‘Ca. M. haemosuis’ in pigs with clinical signs resembling those of Mycoplasma (M) suis and the first description of this novel haemoplasma species outside Asia. On-farm affected pigs were treated with oxytetracycline and non-steroidal anti-inflammatory drugs. Clinical signs improved after implementation of treatment and optimisation of management procedures. This case might indicate that other porcine haemoplasma species than M suis can induce fever and skin alterations and may have an economic impact on affected farms.

Biochemical and haematological changes associated with short periods of work in draught oxen

1989 ◽  
Vol 48 (2) ◽  
pp. 375-384 ◽  
Author(s):  
R. Anne Pearson ◽  
R. F. Archibald
Keyword(s):  
Heart Rate ◽  
Blood Cells ◽  
Inorganic Phosphate ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Blood Levels ◽  
Blood Samples ◽  
Haematological Changes ◽  
Work Done ◽  
Friesian Cattle

ABSTRACTBlood samples were taken from three Brahman × Friesian cattle while they walked for 1 h daily on a treadmill pulling 20 or 25 kg weights suspended in a cage. Heart rate and energy expenditure during work were closely correlated. The work had no significant effect on blood levels of red cells, haemoglobin, packed cell volume, total protein, albumin, glycerol, urea, Mg, Ca, Na, K and chloride. White blood cells, glucose, lactate, free fatty acids, P-hydroxybutyrate and inorganic phosphate were affected by work although the changes were shortlived and values had returned to resting levels 75 min after work finished. The changes were similar in each animal and indicated work done by draught cattle is largely at a submaximal level. Apart from lactate no blood parameters were identified that could be usefully used to compare performance.

Analysis of carbon monoxide

2002 ◽  
Vol 39 (4) ◽  
pp. 378-391 ◽  
Author(s):  
Brian Widdop
Keyword(s):  
Carbon Monoxide ◽  
Chronic Exposure ◽  
Work Place ◽  
Post Mortem ◽  
Blood Samples ◽  
Differential Protein ◽  
Low Level ◽  
Spectrophotometric Methods ◽  
Acceptable Accuracy ◽  
Total Haemoglobin

The degree of exposure to carbon monoxide is most often assessed by measuring the blood carboxyhaemoglobin saturation. This measurement is relevant to investigations of acute accidental or deliberate poisoning and of chronic exposure in a domestic or work place environment. Simple spectrophotometric methods based on differential protein precipitation or dithionite reduction are prone to interference from other haemoglobin pigments and are imprecise for low-level estimations. Automated spectrophotometric devices (CO-oximeters) that estimate simultaneously total haemoglobin, percentage oxyhaemoglobin and percentage carboxyhaemoglobin have acceptable accuracy for carboxyhaemoglobin saturation levels of > 5% and are recommended for most clinical purposes. For the investigation of low-level exposure and the detection of increased haemolysis in neonates, more sensitive methods involving the release of carbon monoxide and its measurement by gas chromatography are required. Gas chromatographic methods are also appropriate when examining post-mortem blood samples where putrefaction or heat stress has resulted in a significant change in haemoglobin composition.

A comparison of some values of blood samples from heart and tail vessels of the Urodele, Taricha granulosa

1970 ◽  
Vol 48 (4) ◽  
pp. 900-901 ◽  
Author(s):  
G. B. Friedmann
Keyword(s):  
Haemoglobin Level ◽  
Cell Count ◽  
Blood Parameters ◽  
Taricha Granulosa ◽  
Blood Samples ◽  
Cell Counts ◽  
Heart Blood ◽  
Two Parameters ◽  
Tail Blood

A comparison of the blood parameters, cell count, and haemoglobin level for heart and tail blood of adult Taricha granulosa is presented. Data from 10 pairs of cell counts and 7 pairs of haemoglobin levels indicate that heart blood yields values only some three-quarters as large as does tail blood for these two parameters.

scholarly journals Physiological Parameters of Endurance Horses Pre- Compared to Post-Race, Correlated with Performance: A Two Race Study from Scandinavia

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
J. Larsson ◽  
P. H. Pilborg ◽  
M. Johansen ◽  
M. T. Christophersen ◽  
A. Holte ◽  
...  
Keyword(s):  
Fluid Intake ◽  
Body Condition Score ◽  
Cooling Water ◽  
Sampling Period ◽  
Blood Parameters ◽  
Physiological Parameters ◽  
Blood Samples ◽  
Heart Score ◽  
Condition Score ◽  
Blood Creatinine

Few studies have investigated the physiological parameters of endurance horses in Scandinavia. Hence, this two race study has focused on the effects of endurance racing in terms of equine clinicopathological blood parameters, heart score, and fluid use. Race A involved 15 horses (120 km). Two pre- and one post-race blood samples were taken, body condition score was assessed in triplicate pre-race, and an ECG was used to determine heart score. Race B involved 16 horses (65–120 km). One pre- and two post-race blood samples were taken. For both races, horse data as well as fluid intake estimates and cooling water were noted. Race A showed that blood haematocrit, albumin, sodium, and triglycerides increased significantly with endurance racing, whilst chloride, glucose, iron, and potassium decreased significantly. In race B, blood creatinine, cholesterol, and inorganic phosphate continued to increase significantly during the first post-race sampling period compared to pre-race levels, whilst iron, which decreased significantly during the race, increased significantly over the two post-race sampling periods. It is concluded that whilst no correlation between heart score and speed was observed, a significant correlation exists between experience and changes in blood parameters with endurance racing and between fluid intake and average speed.

scholarly journals Behavioral and Hematological Response of Clarias gariepinus juvenile exposed to Acute concentrations of Aqueous Extract of Siam Weed (Chloromolaena odorata) leaf as Anesthetic agent

2017 ◽  
Author(s):  
Iheanacho Stanley Chidi ◽  
Nworu Shedrack
Keyword(s):  
Aqueous Extract ◽  
Exposure Time ◽  
Hematological Parameters ◽  
Clarias Gariepinus ◽  
Blood Parameters ◽  
Anesthetic Agent ◽  
Distilled Water ◽  
Chromolaena Odorata ◽  
Blood Samples ◽  
Significant Difference

AbstractThis experiment was carried out to investigate the effect of Siam Weed (chloromelena odorata) on the heamatology of Clarias gariepinus juvenile. A total of one hundred and fifty (150) juvenile of Clarias gariepinus were randomly assigned to different concentrations of C. odorata leave aqueous extract in a completely randomize design (CRD). The concentrations were 50mg/l, 100mg/l, 150mg/l, 200mg/l. Distilled water (0.00 mg/l) was used as the control. The fish exhibited stressful behavior which was higher as the concentration of Chromolaena odorata leave extract increased. There was a gradual decrease with time until a state of calmness, which was subsequently followed by death. The effect on 96hr exposed period was recorded and blood samples collected at 24hr and 96hr interval. Result on hematological parameters revealed significant difference (P<0.05) among treatments with increase in exposure time for all the blood parameters. C. odorata at increased concentrations affected the behavior and hematology of C. gariepinus.

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