Effect of adding anethole to the cryopreservation medium (powdered coconut water) on morphology, kinetics, and oxidative stress of buck sperm

The quality of post-thawing goat sperm is critical to the success of artificial insemination protocols and may be influenced by extenders, cryoprotectants, and antioxidant substances. Therefore, the objective of this study was to evaluate the effects of the antioxidant anethole on goat sperm diluted in preservation medium based on powdered coconut water (ACP-101c) and frozen. For that, each ejaculate was submitted to the following treatments: ACP-101c (control); control plus supplementation with 30, 300, or 2000 μg/mL anethole. The samples were thawed and evaluated for morphology, kinetics, membrane integrity, and reactive oxygen species (ROS). The addition of anethole increased morphological abnormalities (P < 0.05), however, it did not affect sperm kinetics. Flow cytometry analysis showed that sperm cells cryopreserved with 300 μg/mL anethole had lower acrosome integrity than those cryopreserved in other treatments. Evaluation of oxidative stress revealed that cells stored in the presence of 2000 μg/mL anethole had small amounts of ROS when compared to those preserved in the control medium alone or supplemented with 300 μg/mL anethole (P < 0.05). After cryopreservation of sperm with 2000 μg/mL anethole, the highest percentage of viable sperm without ROS was observed (P < 0.05). In conclusion, despite reducing ROS levels, the supplementation of anethole in ACP-101c did not affect sperm kinetics or membrane integrity post-thawing, however, it did cause morphological damage to sperm.

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Cryoprotective Effects of Ergothioneine and Isoespintanol on Canine Semen

Animals ◽

10.3390/ani11102757 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2757

Author(s):

Alexandra Usuga ◽

Irene Tejera ◽

Jorge Gómez ◽

Oliver Restrepo ◽

Benjamín Rojano ◽

...

Keyword(s):

Oxidative Stress ◽

Membrane Integrity ◽

Natural Antioxidants ◽

Ros Production ◽

Straight Line ◽

Functional Membrane ◽

Induced Changes ◽

Kinetics Of ◽

And Oxidative Stress

Sperm undergo oxidative stress due to excessive production of reactive oxygen species (ROS) during cryopreservation. Some unconventional natural antioxidants can reduce ROS-induced changes in cryopreserved canine sperm. This study aimed to identify the cryoprotective effects of ergothioneine and isoespintanol on the quality of thawed canine semen. Twelve ejaculates from six dogs were cryopreserved in a tris-yolk extender without (control) or with 50 (E50), 100 (E100), or 150 (E150) µM ergothioneine or 20 (I20), 40 (I40), or 60 (I60) µM isoespintanol. We evaluated the motility and kinetics of thawed sperm using computerized analysis; determined morphology by eosin-nigrosin staining; functional membrane integrity using hypoosmotic tests, and structural membrane and acrosome integrity; mitochondrial membrane potential by fluorescence microscopy; and ROS production by spectrophotometry. Data were statistically analyzed using mixed models and Tukey tests. E100 increased total (60.6% vs. 49.6%) and progressive (26.4% vs. 20.1%) motility, straight line velocity (41.3 vs. 35.9 µm/s), and rapid sperm (17.6% vs. 12.3%) compared with controls. However, E150 reduced the numbers of hyperactive sperm. E100, I40, and I60 reduced the abnormal morphology and ROS production, and all concentrations of both antioxidants increased acrosomal integrity. We concluded that ergothioneine and isoespintanol reduce deleterious sperm alterations and oxidative stress in thawed canine semen.

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Differential susceptibility of a few members of the nasuta–albomicans complex of Drosophila to paraquat-induced lethality and oxidative stress

Genome ◽

10.1139/g11-049 ◽

2011 ◽

Vol 54 (10) ◽

pp. 829-835 ◽

Cited By ~ 2

Author(s):

Mysore S. Ranjini ◽

Ravikumar Hosamani ◽

Muralidhara ◽

Nallur B. Ramachandra

Keyword(s):

Oxidative Stress ◽

Biochemical Analysis ◽

Reduced Glutathione ◽

Differential Susceptibility ◽

Activity Levels ◽

Oxygen Species ◽

Stress Markers ◽

The Status ◽

Markers Of Oxidative Stress ◽

And Oxidative Stress

The evolution of karyotypically stabilized short-lived (SL) and long-lived (LL) cytoraces in the laboratory have been established and validated through our previous lifespan studies. In the present investigation, we examined the possible reason(s) for the differential longevity among selected members of SL and LL cytoraces, employing the well known paraquat (PQ) resistance bioassay. Exposure of these races to varying concentrations of PQ revealed relatively higher resistance among LL cytoraces than SL cytoraces, as evident by the lower incidence of mortality. Biochemical analysis for endogenous markers of oxidative stress revealed that LL-2 cytorace exhibited lower reactive oxygen species (ROS) and lipid peroxidation (LPO) levels, higher activity levels of superoxide dismutase (SOD), and coupled with higher levels of reduced glutathione (GSH) compared with the levels found in SL-2 cytorace. These findings suggest that the higher susceptibility of SL cytoraces to PQ challenge may be, at least in part, related to the higher endogenous levels of oxidative stress markers. Although the precise mechanisms responsible for the longer longevity among LL cytoraces of the nasuta–albomicans complex of Drosophila merits further investigation, our data suggest that the relatively longer lifespan may be related to the status of endogenous markers that renders them more resistant towards oxidative-stress-mediated lethality, as evident in the PQ assay.

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Oxidative Stress Inside the Pathogenesis of Ascending Aorta Aneurysms: A Clearer Vision for Identifying Promising Biomarkers and Therapeutic Targets

10.20944/preprints202111.0346.v1 ◽

2021 ◽

Author(s):

Carmela Balistreri ◽

Calogera Pisano ◽

Giovanni Ruvolo

Keyword(s):

Oxidative Stress ◽

Risk Factors ◽

Genetic Variants ◽

Complex Disease ◽

Ascending Aorta ◽

Oxygen Species ◽

Aorta Aneurysm ◽

Independent Risk Factors ◽

The Impact ◽

And Oxidative Stress

Ascending aorta aneurysm (AsAA) is a complex disease, currently defined an inflammatory disease. In the sporadic form, AsAA has, indeed, a complex physiopathology with a strong inflammatory basis, significantly modulated by genetic variants in innate/inflammatory genes, acting as independent risk factors and as largely evidenced in our recent studies performed during the last 10 years. Based on these premises, here, we want to revise the impact of reactive oxygen species (ROS) and oxidative stress on AsAA pathophysiology and consequently on the onset and progression of sporadic AsAA. This might consent to add other important pieces in the intricate puzzle of the pathophysiology of this disease with the translational aim to identify biomarkers and targets to apply in the complex management of AsAA, by facilitating the AsAA diagnosis currently based only on imaging evaluations, and the treatment exclusively founded on surgery approaches.

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Advanced Glycation End Products and Oxidative Stress in a Hyperglycaemic Environment

10.5772/intechopen.97234 ◽

2021 ◽

Author(s):

Akio Nakamura ◽

Ritsuko Kawahrada

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Advanced Glycation End Products ◽

Reactive Oxygen ◽

Protein Glycation ◽

Oxygen Species ◽

Advanced Glycation ◽

Glycation End Products ◽

End Products ◽

And Oxidative Stress

Protein glycation is the random, nonenzymatic reaction of sugar and protein induced by diabetes and ageing; this process is quite different from glycosylation mediated by the enzymatic reactions catalysed by glycosyltransferases. Schiff bases form advanced glycation end products (AGEs) via intermediates, such as Amadori compounds. Although these AGEs form various molecular species, only a few of their structures have been determined. AGEs bind to different AGE receptors on the cell membrane and transmit signals to the cell. Signal transduction via the receptor of AGEs produces reactive oxygen species in cells, and oxidative stress is responsible for the onset of diabetic complications. This chapter introduces the molecular mechanisms of disease onset due to oxidative stress, including reactive oxygen species, caused by AGEs generated by protein glycation in a hyperglycaemic environment.

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Inflammatory cytokines and oxidative stress biomarkers in irritable bowel syndrome: Association with digestive symptoms and quality of life

Cytokine ◽

10.1016/j.cyto.2017.05.005 ◽

2017 ◽

Vol 93 ◽

pp. 34-43 ◽

Cited By ~ 43

Author(s):

Razieh Choghakhori ◽

Amir Abbasnezhad ◽

Amin Hasanvand ◽

Reza Amani

Keyword(s):

Quality Of Life ◽

Oxidative Stress ◽

Irritable Bowel Syndrome ◽

Inflammatory Cytokines ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Irritable Bowel ◽

And Oxidative Stress ◽

Syndrome Association

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Methodologies to Assess the Bioactivity of an Herbal Extract on Immunity, Health, Welfare and Production Performance in the Chicken: The Case of Melissa officinalis L. Extract

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.759456 ◽

2021 ◽

Vol 8 ◽

Author(s):

Angélique Travel ◽

Angélique Petit ◽

Perrine Barat ◽

Anne Collin ◽

Camille Bourrier-Clairat ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Cells ◽

Ex Vivo ◽

Redox Balance ◽

Herbal Extracts ◽

Melissa Officinalis ◽

Starting Conditions ◽

Anti Inflammatory ◽

And Oxidative Stress

The potential of herbal extracts containing bioactive compounds to strengthen immunity could contribute to reducing antimicrobial use in poultry. This study aimed at developing a reliable and robust methodological pipeline to assess the ability of herbal extracts to strengthen chicken innate defenses, especially concerning inflammation and oxidative stress. This methodology was applied to Melissa officinalis L. (MEL) extract, recognized for its biological activities including antioxidant and anti-inflammatory properties. Different methods were used to (1). guarantee the quality of MEL extract and its capacity to stimulate the innate immune system; (2). evaluate the relevance of an ex vivo model to mimic inflammatory and oxidative stress challenges to replace LPS injection in chickens; (3). analyse the effects of feed supplemented with MEL extract on inflammation and oxidative stress induced ex vivo; (4). assess the effects of MEL extract on the redox balance, health, welfare and performance in broilers exposed to suboptimal starting conditions through a large-scale approach. The quality of MEL extract preparations, through phytochemical quantification of rosmarinic acid (RA), revealed varying concentrations of RA in the different MEL extracts. RA concentrations remained stable for at least 9 months and in feed three months after incorporating MEL extract. When incubated with chicken cell lines MEL extract showed potential metabolic activation and ability to stimulate immune functions but induced cytotoxicity at high concentrations. The original ex vivo model of inflammation developed on chicken blood cells enabled inflammation and oxidative stress biomarkers to be expressed and revealed antioxidative and anti-inflammatory properties of blood cells from chickens fed MEL extract. The experimental model of chicken suboptimal starting conditions validated beneficial effects of MEL extract on the redox balance and also evidenced improved performance during the growth phase, a tendency for fewer muscle defects but a higher severity of pododermatitis lesions without affecting other welfare indicators. This study grouped methods and tools that could be combined according to the plant extract, the needs of professionals working in poultry production systems and staff responsible for animal health, welfare and feeding.

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Hydrogen peroxide supplementation alleviates the deleterious effects of cadmium on photosynthetic pigments and oxidative stress and improves growth, yield and pods quality of pea (Pisum sativum L.) plants

Acta Physiologiae Plantarum ◽

10.1007/s11738-019-2901-2 ◽

2019 ◽

Vol 41 (7) ◽

Cited By ~ 1

Author(s):

Suzan Sayed ◽

Mohamed Gadallah

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Pisum Sativum ◽

Photosynthetic Pigments ◽

Growth Yield ◽

Pisum Sativum L ◽

And Oxidative Stress

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The Role of MicroRNAs in Diabetes-Related Oxidative Stress

International Journal of Molecular Sciences ◽

10.3390/ijms20215423 ◽

2019 ◽

Vol 20 (21) ◽

pp. 5423 ◽

Cited By ~ 3

Author(s):

Mirza Muhammad Fahd Qadir ◽

Dagmar Klein ◽

Silvia Álvarez-Cubela ◽

Juan Domínguez-Bendala ◽

Ricardo Luis Pastori

Keyword(s):

Oxidative Stress ◽

Target Gene ◽

Cellular Stress ◽

Cellular Damage ◽

Oxygen Species ◽

Non Coding Rnas ◽

And Oxidative Stress

Cellular stress, combined with dysfunctional, inadequate mitochondrial phosphorylation, produces an excessive amount of reactive oxygen species (ROS) and an increased level of ROS in cells, which leads to oxidation and subsequent cellular damage. Because of its cell damaging action, an association between anomalous ROS production and disease such as Type 1 (T1D) and Type 2 (T2D) diabetes, as well as their complications, has been well established. However, there is a lack of understanding about genome-driven responses to ROS-mediated cellular stress. Over the last decade, multiple studies have suggested a link between oxidative stress and microRNAs (miRNAs). The miRNAs are small non-coding RNAs that mostly suppress expression of the target gene by interaction with its 3’untranslated region (3′UTR). In this paper, we review the recent progress in the field, focusing on the association between miRNAs and oxidative stress during the progression of diabetes.

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