Antimicrobial Activities of Acacia nilotica, Ziziphus Jujube Linn and Lawsonia Inermis

2018 ◽  
Vol 26 (2) ◽  
pp. 1
Author(s):  
Aminu Abubakar ◽  
Abdullahi Dandare ◽  
Rabiu Wasagu ◽  
Yerima Musa ◽  
Halima Abubakar
2019 ◽  
Vol 26 (2) ◽  
pp. 1
Author(s):  
A.L. Abubakar ◽  
A Dandare ◽  
I.H. Abubakar ◽  
M Yerima ◽  
R.S.U. Wasagu

Author(s):  
A. L. Abubakar ◽  
A. Dandare ◽  
U. F. Magaji ◽  
I. H. Abubakar ◽  
M. Yerima ◽  
...  

Background: The increasing emergence of resistance to conventional antimicrobial drugs and the complicity of their usage is a serious challenge in Nigeria. In our previous report, it was demonstrated that methanol leaves extracts of Acacia nilotica, Ziziphus jujube Linn and Lawsonia inermis exhibited antibacterial activity against Escherichia coli, Pseudomonas flourecense, Streptococcus and Staphylococcus aureus. Methodology: In this study, agar well diffusion method was employed to assess the antifungal potency of these plant extracts and were tested against Aspergillus flavus, Trichophyton rubrum and Candida albicans. Results: Exclusive of L. inermis extract against T. rubrum at 100 mg/ml (zone of inhibition 34.33±1.89 mm). 100 mg/mL of all the extracts investigated have significantly lower (P<0.05) antifungal activity when compared to standard antifungal drug (Nystatin, 100 mg/ml). The activity of L. inermis against A. flavus was comparatively similar (P>0.05) to the control drug, but significantly higher (P<0.05) against both T. rubrum and C. albicans at 150 mg/ml. Conversely, the antifungal activity of A. nilotica extract against T. rubrum and C. albicans significantly surpass (P<0.05) that of the control drug, while Z. jujube Linn extract activity against C. albicans was comparatively similar (P>0.05) to it, but significantly higher (P<0.05) against T. rubrum. A dose dependent antifungal activity of the plants was observed, and L. inermis extract was the most potent antifungal agent with an MIC and MCF values of 5 mg/ml. Conclusion: This study reveals that L. inermis leaves extract could be used as a sources of potential antifungal agents.


2021 ◽  
Vol 4 (4) ◽  
pp. 348-354
Author(s):  
Aliyu Shehu ◽  
Ahmed Salisu ◽  
Nura S. Gwaram

In this study, the antimicrobial and physicochemical properties of translucent antimicrobial soap prepared using two medicinal plants, Aloe vera gel and Ziziphus jujube leaf extract   was evaluated. The results of the physicochemical analysis showed the pH (7.52±0.02), hardness(1.3 cm±0.02), solubility (0.82 g±0.02) and foamability (6.80 cm±0.03) of the prepared soap and these values were within the acceptable limit  set by WHO/SON which make the prepared soap skin-friendly. Similarly, the antimicrobial screening was carried out on selected Gram-positive and gram-negative bacteria as well as on fungi species using disc diffusion methods and the results showed varying antimicrobial activity at different concentrations ranging from 62.5-500 mg/ml. However, the susceptibility of test bacteria in terms of the zone of inhibition at 500mg/ml of the soap was observed on Staphylococcus lentus (22 mm), Staphylococcus aureus (20mm), Escherichia coli (15 mm), Raoltella ornithinolytica (12mm). The result of antifungal properties was as follows,  Candida albicans (12 mm), Trichophyton rubrum (12 mm), and Aspergillus nigar (10 mm)  at 500 mg/ml concentration. The results when compared to other antimicrobial soap in the market indicate good quality by inhibiting the growth of both Gram-positive, gram-negative bacteria and fungi. The antimicrobial activities exhibited by the soap in this study could be attributed to the presence of phytochemical constituents in the plant extracts, which signify the potential of the soap as an antimicrobial agent. Therefore, these findings confirmed the efficacy of Aloe vera gel and Zizipus jujube extract in traditional medicine.


2020 ◽  
Author(s):  
Abdelhamed A. Abdalla ◽  
Mujahed I. Mustafa ◽  
Abdelrafie M. Makhawi

AbstractThis study was carried out in Khartoum state, during November, 2019. Acacia nilotica was chosen for this study because of its using traditionally in treatment of many diseases. The Phytochemical constitutions of A. nilotica were investigated with aim to identify the antimicrobial effects of this plant. The dried fruit cover of Acacia nilotica was extracted successively with petroleum-ether, chloroform, methanol and distilled water. The phytochemical screening carried out on different extracts of Acacia nilotica fruit cover showed high amount of Glycosides, Flavonoids and Terpenoids (in all extracts) and moderate amount of Tannin in methanol and distilled water extracts, Alkaloids (acid and base) in methanol extract and Saponin in methanol and petroleum-ether extracts. The antimicrobial activity of extracts were evaluated against four standard bacteria species (gram positive bacteria; Staphylococcus aureus, Bacillus subtilis) and (gram negative bacteria; Pseudomonas aeruginosa, Escherichia coli). The plates were inoculated for sensitivity testing, minimum inhibitory concentration (MIC) was measured. The results of antimicrobial investigation show that the distilled water and methanolic extracts inhibited the growth of all microorganisms (Specified by the zone of inhibition). The results provide promising baseline information for potential use of these crude extracts in drug development programs in the pharmaceutical industries.


Author(s):  
Osan Bahurmiz ◽  
Rosma Ahmad ◽  
Noryati Ismail ◽  
Frederick Adzitey ◽  
Shaida-Fariza Sulaiman

The antimicrobial activity of various plant extracts on Pseudomonas bacteria isolated from spoiled chilled tilapia (Oreochromis sp.) was evaluated in this study. In the first stage of this study, red tilapia was subjected to chilled storage (4°C) for 3 weeks, and spoilage bacteria were isolated and identified from the spoiled fish. Pseudomonas was the dominant bacteria isolated from the spoiled fish and further identification revealed that P. putida, P. fluorescens and Pseudomonas spp. were the main species of this group. In the second stage, methanolic extracts of 15 selected plant species were screened for their antimicrobial activity, by agar disc diffusion method, against the Pseudomonas isolates. Results indicated that most of the extracts had different degrees of activity against the bacterial isolates. The strongest activity was exhibited by bottlebrush flower (Callistemon viminalis) extract. This was followed by extracts from guava bark (Psidium guajava) and henna leaf (Lawsonia inermis). Moderate antimicrobial activities were observed in extracts of clove (Syzygium aromaticum), leaf and peel of tamarind (Tamarindus indica), cinnamon bark (Cinnamomum zeylanicum), wild betel leaf (Piper sarmentosum) and fresh thyme (Thymus spp.). Weak or no antimicrobial activity was observed from the remaining extracts. The potential antimicrobial activity shown by some plant extracts in this study could significantly contribute to the fish preservation.


Author(s):  
Ashraf Tawfiek Soliman ◽  
Fatma A. Hamada

This survey was conducted on 4 tribes (Ababda, Bisharia, Nubian and Rashayda) live in the south of the Nile and the Eastern Desert of Egypt with the aim to document and compare the traditional herbal medicines and assess the relationships among these tribes. A total of 180 interviews were conducted with the Bedouins and herb healers. Thirty-nine species belonged to 36 genera and 27 families were employed. Fabaceae and Poaceae and Rutaceae were the species-rich families. The used wild species comprised 43.6%, cultivated species (38.5%) and the imported from herbalist shops (17.9%). The leaves were the most used parts (31%), followed by stems and fruits with about 22% each. Distinct species included Acacia nilotica is used in the treatment of dental pain with use value 33.3%, Cymbopogon schoenanthus subsp. proximus in treatment of both cough or headache with use values 35 and 30.6% and a combination of Acacia nilotica with Lawsonia inermis in the treatment of sore throat with use value 22.2%. The highest similarity was recorded between Nubian and Rashayda tribes (55.3%), Ababda and Bisharia (46.8%). Diarrhea and headache were the most popular diseases with 7 different treatments, cough and dental pains with 6 treatments.


Author(s):  
Shaista Yaqoob ◽  
Fatima Waheed ◽  
Qurban Ali ◽  
Arif Malik

With the growing era, there is tremendous need to produce high potential antimicrobial drugs and medicines from various herbaceous plants and natural resources has been increasing due to increasing multiple drug resistance in pathogens. An experiment was performed to know about antimicrobial activities of various plant extracts. These activities were tested by using agar diffusion method. The bacterial as well as fungal strains were collected and were cultured on agar plates. After that these plates were left in incubator for 24 hours at 37°C to develop zones clearly all round the plant extracts. The activities of bacteria and fungi were determined by using inhibition diameter zones. The clear inhibition zones were found against bacterial strains in study. Antimicrobial activity of acetone, n-hexane and water extracts viz. Acacia nilotica and Artemissia vulgaris tested against Pseudomonas aeruginosa, Bacillus subtils and E. coli as well as Aspergillus niger and Fusarium oxysporum. Acetone extracts were found to be more effective as compared with n-hexane while water extracts show no activity.  


2019 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Kamalu Abdullahi Alhassan ◽  
Aisha Shuaibu Indabawa ◽  
M. Manjur Shah

Despite tremendous progress in human medicines infectious diseases caused by bacteria, fungi, viruses and parasites are still a major threat to public health. Their impact is particularly large in developing countries due to relative unavailability of medicines and emergence of widespread drugs resistance (Zampini 2009).The aim and objectives of this research work was designed to carried out the Phytochemical analysis, proximate composition and evaluate the antimicrobial activities of the Ziziphus jujube and Ziziphus spina christi leaves against clinical bacterial isolates (Escherichia coli, Staphloccoccus  aureus and Klebsiella  pneumoniae) as they were found out that since ancient times to date, they are used in treating various antimicrobial, ailmentand disorders etc. The results of the research shows that, the effect of antibacterial activities of both aqueous and ethanolic extracts of Ziziphus jujube (e extract conc. aqueous; F=119.37, Bacterial extract conc. aqueous; F=1.00 and extract conc. ethanol; F=15.74, Bacterial extract conc. ethanol; F=0.59) are reciprocal proportional to their counterpart, Ziziphus spina-christi (extract conc. aqueous; F=54.96, Bacterial extract conc. aqueous; F=0.94 and extract conc. ethanol; F=81.11, Bacterial extract conc. ethanol; F=1.37). In sum, the minimum inhibitory concentration of Ziziphus jujube shows that, the aqueous extract has M.I.C at range of 11.7 to 8.7mg/ml on all tested bacteria but the ethanolic extract has M.I.C of 14.8 to 8.2mg/ml range on E.coli, Klepsiella spp and S. aureus. While, the minimum inhibitory concentraton of Ziziphus spina-christi shows that the M.I.C of aqueous extract range of 12.8 to 8.3mg/ml on E. coli, Klepsiella spp and S.aureus. But, Ziziphus spina-christi M.I.C of ethanolic extract is 13.5 to 8.8mg/ml on all the tested bacteria. In sum, Zizuphus spina-christi has lower nutritional content and low MIC ethanolic extract than that of Ziziphus jujube.


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