scholarly journals Role of some rhizospheric Pseudomonas on the growth and physiology of broad bean (Vicia faba) under salt stress conditions

2019 ◽  
Vol 72 (4) ◽  
Author(s):  
Boubaker Idder ◽  
Rachid Djibaoui ◽  
Hocine Abdelhakim Reguieg Yssaad ◽  
Abdelhak Djoudi

Salt stress affects the development and growth of plants in various ways as a result of its effect on water relationships, photosynthesis, and nutrient absorption by physiological and biochemical processes. Consequently, several researchers have increasingly studied the effect of plant growth promoting bacteria (PGPR) as promoters and enhancers under saline environment. The main goals of this study were to examine the manifested response of the broad bean plant under saline conditions and to evaluate the role of some <em>Pseudomonas</em> isolates in improving plant tolerance to salt stress. Three <em>Pseudomonas</em> strains were isolated (P1 and P7 from a saline soil and P15 from a vineyard soil). These isolates were screened by salinity and used as inoculums in <em>Vicia faba</em> plants (OTONO variety) irrigated with two saline solutions (NaCl; 100 and 150 mM L<sup>−1</sup>) and one without salinity. The results show that salinity decreased the fresh weight, total chlorophyll content, and the Na<sup>+</sup>/K<sup>+</sup> ratio, but it increased proline accumulation in inoculated and noninoculated plants. The inoculation of <em>V. faba</em> plants with P1, P7, and P15 strains significantly increased the production of fresh biomass in the presence and absence of salt stress, and positively affected the accumulation of proline and the Na<sup>+</sup>/K<sup>+</sup> ratio. The inoculation with bacterial strains increased the total chlorophyll content in plants at all salt treatment levels, especially the P1 strain that showed a significant effect.

2020 ◽  
Vol 5 (2) ◽  
pp. 144-150
Author(s):  
Sharmin Akter ◽  
Dilruba Yeasmin Jharna ◽  
Sujan Kanti Mali ◽  
Abu Sayeed

The present study was accomplished to find out the effects of salinity on germination, growth, physiological and biochemical processes of two different groundnuts (Arachis hypogaea L.), varieties viz., Dacca-1, and Zhinga groundnut. The experiments consisted of five salinity treatments viz., control (no salinity), 3, 6, 9, and 12dS/m during germination and vegetative stage of groundnut varieties. Results showed that germination percentage of both the groundnut varieties was significantly decreased with the increase of salt concentration. During the germination stage, maximum shoot and root length, shoot and root fresh weight and shoot and root dry weight of groundnut varieties were recorded from Zhinga groundnut variety at salt stress. The same result occurred during the vegetative stage of Dacca-1 variety while the Zhinga groundnut variety showed maximum parameters than Dacca-1. Fresh weight, as well as dry weight, of shoots and roots of both the groundnut varieties was also decreased with the increase of salt concentrations while leaf proline concentrations were increased among these varieties. Salinity caused significant (P<0.001) reduction in chlorophyll a, chlorophyll b, and total chlorophyll content in both the genotypes. Between two genotypes, Zhinga groundnut recorded higher chlorophyll a, chlorophyll b and total chlorophyll content than Dacca-1. From the studies, it is concluded that the groundnut genotype Zhinga was identified as the tolerant genotype to salt stress than Dacca-1. Overall results indicate that high salinity condition is not suitable for growing the groundnut.


Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 445-462 ◽  
Author(s):  
Amir Khalatbari ◽  
Hawa Jaafar ◽  
Amir Khalatbari ◽  
Maziah Mahmood ◽  
Radziah Othman

Plants experience different abiotic stresses under natural conditions including salinity, water deficit, low temperature and high light. Once plants are exposed to these stresses they might have a variety of responses physiologically and biochemically. In this study, we test this hypothesis in wild type Col-0 and vte4 mutant of Arabidopsis thaliana by measuring major secondary metabolites alongside with total chlorophyll content under different abiotic stresses namely salt stress, water stress and prolonged water deficiency. These stresses were imposed to the plants in separate experiments in which each treatment was replicated three times in a complete randomized design with factorial arrangement. It was concluded that under all abiotic stresses wild type Col-0 Arabidopsis plants showed stronger performance in terms of all major metabolites compared to vte4 mutant. ?-tocopherol deficiency in vte4 mutant plants led to lower accumulation of proline, total protein and total amino acids as well as starch and total sugars in comparison with wild type A. thaliana. Furthermore, all five secondary metabolites obtained the highest value under 100mM NaCl concentration (Salt stress), under 50% of field capacity (water stress) and under 8 days of water withholding (prolonged water deficiency). Wild type Col-0 resulted in higher level of total chlorophyll content under all abiotic stresses compared to mutant plants. Therefore, our results suggested that the loss of ?-tocopherol in vte4 mutant A.thaliana under different abiotic stresses affected the efficiency and the stability of central metabolism and photosynthetic apparatus.


2013 ◽  
Vol 5 (3) ◽  
pp. 364-370 ◽  
Author(s):  
Elnaz SOLEIMANY-FARD ◽  
Khodayar HEMMATI ◽  
Ahmad KHALIGHI

Keeping quality and length of vase life are important factors for evaluation of cut flowers quality, for both domestic and export markets. Studding the effect of pre- and post-harvest salicylic acid applications on keeping quality and vase life of cut alstroemeria flowers during vase period is the approach taken. Aqueous solutions of salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM were sprayed to run-off (approximately 500 mL per plant), about two weeks before flowers harvest. The cut flowers were harvested in the early morning and both of cut flowers treated (sprayed) and untreated were kept in vase solutions containing salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM. Sucrose at 4% was added to all treatments as a base solution. The changes in relative fresh weight, water uptake, water loss, water balance, total chlorophyll content and vase life were estimated during vase period. The results showed that the relative fresh weight, water uptake, water balance, total chlorophyll content and vase life decreased significantly while the water loss increased significantly during experiment for all treatments. A significant difference between salicylic acid and control treatments in all measured parameters is observed. During vase period, the salicylic acid treatments maintained significantly a more favourable relative fresh weight, water uptake, water balance, total chlorophyll content and supressed significantly water loss, as compared to control treatment. Also, the results showed that the using salicylic acid increased significantly the vase life cut alstroemeria flowers, over control. The highest values of measured parameters were found when plants were treated by pre + post-harvest application of salicylic acid at 3 mM. The result revealed that the quality attributes and vase life of cut alstroemeria flowers were improved by the use of salicylic acid treatment.


2021 ◽  
Vol 6 (2) ◽  
pp. 86-102
Author(s):  
Felipe Roberto Flores-de la Rosa ◽  
◽  
Ricardo Santillán-Mendoza ◽  
Cynthia Guadalupe Rodríguez-Quibrera ◽  
Antonio Martínez-Ruiz ◽  
...  

Persian lime is economically important for Mexico. However, the disease known as Huanglongbing (HLB) causes chlorosis symptoms in the foliage that affect the growth and decreases the yield of the trees. The expression of chlorosis symptoms is associated with starch accumulation and inhibition of antioxidant activity. The objective of the present work was to determine the effect of the application of three resistance elicitors on the expression of three antioxidant genes and on starch and chlorophyll content in Persian lime trees with HLB. Salicylic acid (SA), jasmonic acid (JA) or gamma-aminobutyric acid (GABA) were applied every eight days for nine weeks using a completely randomized design and a one-way ANOVA was performed for the analysis. Expression of APX, CAT and SOD genes was quantified at week nine at four different times. Starch and total chlorophyll content was estimated every three weeks by spectrophotometric methods. The application of the elicitors significantly increased the expression of the three genes, with SA and GABA generating the greatest increase at different times of application. No difference was found in starch and total chlorophyll content at most of the times evaluated. The use of resistance elicitors is promising in the management of HLB in plots already infected, seeking to extend the productive life of the orchards and thus counteract the economic losses caused by HLB.


2020 ◽  
Vol 9 (4) ◽  
pp. 426
Author(s):  
Brogina Mayank Dini ◽  
Luh Putu Trisna Darmayanti ◽  
I Ketut Suter

This research was conducted with the aim to determine the effect of comparison of mackerel with gonda vegetable on meatballs characteristics and get a comparison of mackerel with specific gonda vegetable that can produce meatballs with the best characteristics. This study uses a Completely Randomized Design (CRD) with treatment that is the comparison of mackerel with gonda vegetable consisting of 6 levels: 100%: 0%; 95%: 5%; 90%: 10%; 85%: 15%; 80%: 20%; 75%: 25%. Each treatment was repeated 3 times so that obtained 18 units. The data is then analyzed by analysis of variance and if the treatment influences the proposed variable then it is continued by Duncan. The results showed that the comparison of mackerel and gonda vegetable had a very significant effect on water content, ash content, fat content, protein content, carbohydrate content, total chlorophyll content, antioxidant capacity, color (scoring), texture, aroma, overall acceptance and no significant effect for texture, aroma and taste. Comparison of 80% mackerel and 20% gonda vegetables had the best characteristics, with 62.99% water content, 1.56% ash content, 1.28% fat content, 8.75% protein content, 25.43% carbohydrate content, 18.39% total chlorophyll content, antioxidant capacity 25.63%. Sensory properties obtained are color (rather green, rather like), texture (rather like), flavor (liked) and overall acceptance (like).


2021 ◽  
Vol 3 (2) ◽  
pp. 37-44
Author(s):  
Selvia Dewi Pohan

Water spinach (Ipomoea reptans Poir) had been identified as a nutritious vegetable with high demand in Indonesia. Besides, this plant also has been evidenced to play an important role in environmental cleaning as phytoremediator. The study about the effect of organic fertilizers on the growth and yield of water spinach (Ipomoea reptans Poir) has been conducted in the Green House of Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. The study aims to investigate the most effective type of fertilizers and the dose for water spinach’s (Ipomoea reptans Poir) growth and yield. A Completely Randomized Factorial Design was designed for the experiment with two factors and three repetitions. The first factor is the type of fertilizer (chicken manure, cow manure, and compost), and the second factor is the dose of the fertilizer (1:1, 2:1, and 3:1). The parameters such as plant height, number of shoots, number of leaves, fresh weight, dry weight, leaf’s total chlorophyll content, and water content were measured to evaluate plant growth and yield. The General Linear Model used SPSS 21 programs was applied to analyze the collected data. Study results revealed that cow manure increased plant growth and yield significantly with dose 2:1 as the finest treatment, followed by dose 3:1. Cow manure also increased total chlorophyll content (8.0574c mg. L-1), with the most suitable dose was 2:1 (8.2807 mg. L-1). The plant’s water content tended to be high in chicken manure (93%), and the lower water content was in cow manure with dose 3:1 (87.5%).


Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 246 ◽  
Author(s):  
Saber Avestan ◽  
Mahmood Ghasemnezhad ◽  
Masoud Esfahani ◽  
Caitlin S. Byrt

Silicon application can improve productivity outcomes for salt stressed plants. Here, we describe how strawberry plants respond to treatments including various combinations of salt stress and nano-silicon dioxide, and assess whether nano-silicon dioxide improves strawberry plant tolerance to salt stress. Strawberry plants were treated with salt (0, 25 or 50 mM NaCl), and the nano-silicon dioxide treatments were applied to the strawberry plants before (0, 50 and 100 mg L−1) or after (0 and 50 mg L−1) flowering. The salt stress treatments reduced plant biomass, chlorophyll content, and leaf relative water content (RWC) as expected. Relative to control (no NaCl) plants the salt treated plants had 10% lower membrane stability index (MSI), 81% greater proline content, and 54% greater cuticular transpiration; as well as increased canopy temperature and changes in the structure of the epicuticular wax layer. The plants treated with nano-silicon dioxide were better able to maintain epicuticular wax structure, chlorophyll content, and carotenoid content and accumulated less proline relative to plants treated only with salt and no nano-silicon dioxide. Analysis of scanning electron microscopic (SEM) images revealed that the salt treatments resulted in changes in epicuticular wax type and thickness, and that the application of nano-silicon dioxide suppressed the adverse effects of salinity on the epicuticular wax layer. Nano-silicon dioxide treated salt stressed plants had increased irregular (smoother) crystal wax deposits in their epicuticular layer. Together these observations indicate that application of nano-silicon dioxide can limit the adverse anatomical and biochemical changes related to salt stress impacts on strawberry plants and that this is, in part, associated with epicuticular wax deposition.


Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 663 ◽  
Author(s):  
Jianhong Ren ◽  
Jun Ye ◽  
Lina Yin ◽  
Gouxia Li ◽  
Xiping Deng ◽  
...  

Melatonin has been confirmed extensively for the positive effects on increasing plant tolerance to various abiotic stresses. However, the roles of melatonin in mediating different stresses still need to be explored in different plants species and growth periods. To investigate the role of melatonin in mitigating salt stress, maize (Zea mays L.) seedlings growing in hydroponic solution were treated with 100 mM NaCl combined with or without 1 μM melatonin. Melatonin application had no effects on maize growth under normal condition, while it moderately alleviated the NaCl-induced inhibition of plant growth. The leaf area, biomass, and photosynthesis of melatonin-treated plants were higher than that of without melatonin under NaCl treatment. The osmotic potential was lower, and the osmolyte contents (including sucrose and fructose) were higher in melatonin-treated plants. Meanwhile, the decreases in Na+ content and increases in K+/Na+ ratio were found in shoots of melatonin-applied plant under salt stress. Moreover, both enzymatic and nonenzymatic antioxidant activities were significantly increased in leaves with melatonin application under salt treatment. These results clearly indicate that the exogenous melatonin-enhanced salt tolerance under short-term treatment could be ascribed to three aspects, including osmotic adjustment, ion balance, and alleviation of salt-induced oxidative stress.


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