scholarly journals Insights into the in vitro germicidal activities of Acalypha indica

2017 ◽  
Vol 30 (1) ◽  
pp. 26-31
Author(s):  
Md. Shahedur Rahman ◽  
Riad Hossain ◽  
Forhad Karim Saikot ◽  
Shaikh Mizanur Rahman ◽  
Subbroto Kumar Saha ◽  
...  
Keyword(s):  
Acalypha Indica

scholarly journals Phytochemical Profile, Free Radical Scavenging and Anti-Inflammatory Properties of Acalypha Indica Root Extract: Evidence from In Vitro and In Vivo Studies

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6251
Author(s):  
Ravi Sahukari ◽  
Jyothi Punabaka ◽  
Shanmugam Bhasha ◽  
Venkata Subbaiah Ganjikunta ◽  
Shanmugam Kondeti Ramudu ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vivo Studies ◽  
Root Extract ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Acalypha Indica

In our in vitro and in vivo studies, we used Acalypha indica root methanolic extract (AIRME), and investigated their free radical scavenging/antioxidant and anti-inflammatory properties. Primarily, phytochemical analysis showed rich content of phenols (70.92 mg of gallic acid/g) and flavonoids (16.01 mg of rutin/g) in AIRME. We then performed HR-LC-MS and GC-MS analyses, and identified 101 and 14 phytochemical compounds, respectively. Among them, ramipril glucuronide (1.563%), antimycin A (1.324%), swietenine (1.134%), quinone (1.152%), oxprenolol (1.118%), choline (0.847%), bumetanide (0.847%) and fenofibrate (0.711%) are the predominant phytomolecules. Evidence from in vitro studies revealed that AIRME scavenges DPPH and hydroxyl radicals in a concentration dependent manner (10–50 μg/mL). Similarly, hydrogen peroxide and lipid peroxidation were also remarkably inhibited by AIRME as concentration increases (20–100 μg/mL). In vitro antioxidant activity of AIRME was comparable to ascorbic acid treatment. For in vivo studies, carrageenan (1%, sub-plantar) was injected to rats to induce localized inflammation. Acute inflammation was represented by paw-edema, and significantly elevated (p < 0.05) WBC, platelets and C-reactive protein (CRP). However, AIRME pretreatment (150/300 mg/kg bodyweight) significantly (p < 0.05) decreased edema volume. This was accompanied by a significant (p < 0.05) reduction of WBC, platelets and CRP with both doses of AIRME. The decreased activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in paw tissue were restored (p < 0.05 / p < 0.01) with AIRME in a dose-dependent manner. Furthermore, AIRME attenuated carrageenan-induced neutrophil infiltrations and vascular dilation in paw tissue. For the first time, our findings demonstrated the potent antioxidant and anti-inflammatory properties of AIRME, which could be considered to develop novel anti-inflammatory drugs.

scholarly journals Identification of the Anthelmintic Power of Anting-anting Leaf and Starfruit Leaf

2020 ◽  
Vol 4 (5) ◽  
pp. 140-147
Author(s):  
Sri Sulami Endah Astuti
Keyword(s):  
Ascaris Suum ◽  
Block Design ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Medical Laboratory ◽  
Time Of Death ◽  
Time Required ◽  
Acalypha Indica

Ascariasis is an intestinal infection caused by the parasitic worm Ascaris lumbricoides. Starfruit leaf (Averrhoa bilimbi) and leaf anting-anting (Acalypha indica L) is a plant that is often used as a worm remedy in the community. Starfruit leaves (Averrhoa bilimbi) and Leaf anting-anting (Acalypha indica L) contain several compounds that have the potential as anthelmintics, namely saponins, tannins and flavonoids. The purpose of this study was to determine the effect of ethanol extention of starfruit leaf leaves (Averrhoa bilimbi) and leaf anting-anting (Acalypha indica L) as anthelmintic on the time of death of Ascaris suum Goeze worms in vitro. The method in this study is experimental with post test only group design. Test animals from the study are Ascaris suum. The study was conducted at the Parasitology Laboratory, Medical Laboratory Department, Poltekkes Kemenkes Surabaya in 2019. This study used 6 treatment groups, NaCl 0.9% as a negative control and pirantel pamoate 0.25% as a positive control and ethanol extract of wuluh starfruit leaf and ethanol extract of Anting-anting with concentrations of leaves. 40%, 60%, 80% and 100%. Data were analyzed using the Kolmogrov-Smirnov test, the Anova two way Block Design test, then continued using the Post Hoc test to determine differences between blocks and between treatments, against the time of Ascaris suum worm's death. The average time of death of Ascaris suum caused by ethanol extract of starfruit leaves concentration of 40% for 492.3 minutes and ethanol extract of leaf anting-anting 396.5 minutes, concentration of 60% ethanol extract of wuluh starfruit leaves for 278.5 minutes and leaf extract ethanol leaf anting-anting for 283.5 minutes, 80% concentration of ethanol extract of wuluh starfruit leaf for 141.8 minutes and ethanol extract of anting-anting leaf for 174 minutes, 100% concentration of ethanol extract of wuluh starfruit leaves for 66.75 minutes and extract ethanol leaf anting-anting for 92 minutes. So it can be concluded that the ethanol extract of starfruit leaves has an anthelmintic effect on the Ascaris suum worm more optimum than the ethanol extract of the Anting-anting leaves, because the time required is close to positive control that is 60 minutes. Keywords: anthelmintic; Ascaris suum; starfruit leaf; anting-anting leaf

scholarly journals Phytochemical Investigation and In vitro Antimalarial Activity of Acalypha indica (L.) and Cocculus hirsutus (L.) From Prakasam District, Andhra Pradesh, India

2018 ◽  
Vol 11 (4) ◽  
pp. 2123-2134
Author(s):  
Pasumarthi Brahmam ◽  
Kanikaram Sunita
Keyword(s):  
Ethyl Acetate ◽  
Andhra Pradesh ◽  
Antimalarial Activity ◽  
Standard Laboratory ◽  
Phytochemical Screening ◽  
Ic50 Values ◽  
Cocculus Hirsutus ◽  
Acalypha Indica ◽  
Ethyl Acetate Extracts

The present study, report the phytochemical analysis and in vitro antimalarial activity of plants Acalypha indica (L.) and Cocculus hirsutus (L.). The A. indica and C. hirsutus plant was collected from Kadaparajupalle at Dornala mandal, Prakasam district, Andhra Pradesh, India. Leaf, stem bark and root crude extracts prepared in Soxhlet apparatus with chloroform, ethyl acetate and methanol solvents. The preliminary phytochemical screening of these extracts was conducted by following the standard methods. These extracts were tested for in vitro antimalarial activity against 3D7 and K1 strains of Plasmodium falciparum by standard laboratory protocol. In vitro cytotoxicity of the extracts was also tested by following standard laboratory method. The phytochemical screening has revealed the presence of alkaloids, saponins, terpenoids & steroids, tannins, anthocyanidins, phenolic compounds, coumarins, quinones, resins and glycosides. Amongst all the extracts screened for antimalarial activity, the leaf chloroform and ethyl acetate extracts of A. indica shown IC50 values of 3.34 µg/mL and 3.71 µg/mL respectively against 3D7 strain; the leaf chloroform and ethyl acetate extracts of A. indica shown IC50 values of 1.47 µg/mL and 2.32 µg/mL respectively against K1 strain; the root chloroform and methanol extracts of C. hirsutus shown IC50 values of <0.78 µg/mL and 3.714 µg/mL respectively against 3D7 strain; the root chloroform and methanol extracts of C. hirsutus shown IC50 values of <0.78 µg/mL and 2.10 µg/mL respectively against K1 strain. Thus, the above extracts have shown very active antimalarial activity against 3D7 and K1 strains. And all the extracts were non-toxic showing CC50 values of >20 µg/mL against Vero cell line. The presence of high alkaloids, flavonoids and terpenoids of the plant extracts suggest their antioxidant potential and justifies their therapeutic action which could be used for the drug formulation. The chloroform root extract of C. hirsutus has shown excellent antimalarial activity which can be used for the development of new antimalarial drug policies.

scholarly journals Acalypha indica and Curcuma longa plant extracts mediated ZnS nanoparticles

2019 ◽  
Vol 16 (2) ◽  
pp. 174-182
Author(s):  
M. Sathish Kumar ◽  
M. Saroja ◽  
M. Venkatachalam
Keyword(s):  
Plant Extract ◽  
Curcuma Longa ◽  
Cost Effective ◽  
Inhibition Zone ◽  
Precipitation Method ◽  
Diffusion Method ◽  
Zns Nanoparticles ◽  
Co Precipitation ◽  
Acalypha Indica

The development of biomedical electronics, biosynthesis of ZnS nanoparticles(NPs) much attracted researchers, due to an eco-friendly and cost-effective routes forsynthesisZnSnanoparticles. In this present work, ZnS NPswas synthesized by using acalypha indica and curcuma longa plant extract using chemical co-precipitation method. The structural, morphological, element composition of biosynthesisZnS NPs was characterized by XRD, SEM and EDAX respectively. Optical and photoluminescence (PL) properties were evaluated by UV Visible spectroscopy. The formation of inhibition zone diameter against human pathogenic microorganisms was screened by in vitro disc diffusion method. From this investigation formation of inhibition zones clearly shows biosynthesizedZnS NPs have high antimicrobial activity against tested organisms, especially curcuma longa plant extract mediated ZnS NPs was formed maximum inhibition against all the tested microorganism.

scholarly journals The Potential of Anting – Anting (Acalypha indica L.) Leaf Extract as Anti-Scabies to Sarcoptes scabiei var. Cuniculi in vitro

2019 ◽  
Vol 3 (2) ◽  
pp. 67
Author(s):  
Luluk Tri Astuti ◽  
Rahmi Sugihartuti ◽  
Lianny Nagoi ◽  
Nunuk Dyah Retno Lastuti ◽  
Dewa Ketut Meles ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Leaf Extract ◽  
Mortality Rates ◽  
Sarcoptes Scabiei ◽  
Lethal Concentration ◽  
Lethal Time ◽  
Acalypha Indica

The Aim of research to determine the potential of anting-anting (Acalypha indica L.) leaf extract as anti-scabies to Sarcoptes scabiei var. Cuniculi in vitro by observing the mortality rates and lethal concentration of Sarcoptes scabiei. There were 5 treatments namely DMSO 0.5% (PN). Amitraz (P0), anting-anting (Acalypha indica L.) leaf extract concentratrion of 6,25% (P1), 12,5% (P2) and 25% (P3). Observations were carried out for 8 hours with stereomicroscope. The data  was analyzed using rpobit analysis with SPSS 20.0 software. The results showed that the fastest mortality rate of S. Scabiei was group p3 at 4.6 mites/hour., then P0, P2, P1 and PN respectively 3.5, 3.1, 1.9, and 1.3 mites/ hour. The smallest concentration of anting-anting (Acalypha indica L.) leaf extract was not significantly different compared to amitraz  for killing of 50% and 90% S. Scabiei was 12.5% with lethal time (LT50) 1.82 hours and (LT90) 3.69 hours. In conclusion, anting-anting (Acalypha indica L.) leaf extract has mortality rate against S. Scabiei var. Cuniculi of 3.1 mites/hour, lethal concentration of 12.5% and potential as anti-scabies in vitro.

scholarly journals Bacteriostatic and bactericidal activities of Acalypha indica L. plant leave extracts on bacteria shigella disenteriae type 1 in vitro

2021 ◽  
Vol 8 (6) ◽  
pp. 304-309
Author(s):  
Abdul Majid ◽  
Frans Salesman
Keyword(s):  
Experimental Study ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Shigella Dysenteriae ◽  
Herbal Extract ◽  
Bactericidal Activities ◽  
Acalypha Indica ◽  
Shigella Dysenteriae Type

This study aimed to analyze the bacteriostatic and bactericidal activity of Acalypha indica L. extract against Shigella dysenteriae type 1 bacteria in vitro. This research is a laboratory experimental study consisting of six concentration treatments, namely: (K = 0/ml, P1 = 100/ml, P2 = 200/ml, P3 = 400/ml, P4 = 800/ml, P5 = 1600 mg/ml ), and five replications each. The results showed that the herbal extract of Acalypha indica L. was bacteriostatic (inhibiting) at a concentration of 400 mg/mm, and bactericidal (killing) the bacteria Shigella dysenteriae type 1, which was tested in vitro at a concentration of at least 800 mg/ml. It is necessary to study the effect of Acalypha indica L. herbal extract from Timor in the serum of Rattus norwegicus against bacteria that cause bloody diarrhea (EHEC O157:H7, and Shigella dysenteriae type 1), MRSA bacteria, and other pathogenic bacteria.

Efficacy of Chemical Fungicides and Plant Extracts Against Anthracnose Pathogens of Capsicum Frutescens L.

2021 ◽  
Vol 50 (2) ◽  
pp. 437-440
Author(s):  
Md Abu Raihan Rony ◽  
Sarowar Hosen ◽  
MA Bashar
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Capsicum Frutescens ◽  
Lippia Alba ◽  
Colletotrichum Dematium ◽  
Heliotropium Indicum ◽  
Chemical Fungicides ◽  
Acalypha Indica ◽  
In Vitro Antifungal Activity

To evaluate the in vitro antifungal activity of five fungicides viz., Capvit 50 WP, Contaf 5 EC, Greengel 72 WP, Knowin 72 WP and Score 250 EC as well as five plant extracts viz., Acalypha indica L., Azadirachta indica A. Juss., Heliotropium indicum L., Lippia alba L. and Magnolia champaca L. at different concentrations were selected against Colletotrichum dematium and C. gloeosporioides following poisoned food technique. Among the five fungicides, Capvit 50 and Score 250 showed the complete growth inhibition of C. dematium whereas Contaf 5 EC showed the complete growth inhibition of C. gloeosporioides at all the concentrations. Contaf 5 was found as the most effective inhibitor against C. dematium. On the other hand, Knowin 72 and Score 250 were found as the most effective fungicides against C. gloeosporioides. A. indica and L. alba showed highest radial growth inhibition of C. dematium and C. gloeosporioides at 20% concentration. Moreover, A. indica and M. champaca also showed desired growth inhibition of the test pathogens. This study paves the way for the management of C. dematium and C. gloeosporioides causing anthracnose disease of chilli. Bangladesh J. Bot. 50(2): 437-440, 2021 (June)

Aqueous extract of Acalypha indica leaves for the treatment of Psoriasis: In-vitro studies

2017 ◽  
Vol 6 (04) ◽  
pp. 5360 ◽  
Author(s):  
Rajkiran Reddy Banala ◽  
Satish Kumar Vemuri ◽  
Gurava Reddy A.V. ◽  
Subbaiah G.P.V.*
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Annexin V ◽  
In Vitro Study ◽  
In Vitro Models ◽  
Fluorescence Studies ◽  
Acalypha Indica

Psoriasis is a chronic inflammatory skin disorder characterized by rapid proliferation of keratinocytes and incomplete keratinization. Discovery of safer and more effective anti-psoriatic drugs remains an area of active research at the present time. A431 and B16-F10 cell lines were used as in vitro models. In the present study, we aimed at assessing the Anti-psoriatic activity of aqueous extract of Acalypha indica. We analyzed the efficiency of A. indica leaf extract in inducing cell death and apoptosis in these cell lines. The cell death (Propidium iodide) and apoptosis (Annexin V) was assessed by fluorescence studies and we observed 80% of cell death and 75% of apoptosis in both cell lines. Therefore, this in vitro study suggested that the leaf extract is capable of serving as anti-psoriasis agent or compound.

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