The relationship between antibrowning, anti-radical and reducing capacity of Brassica and Allium extracts

Aqueous vegetable extracts from Allium and Brassica families were assayed for antibrowning capacity and related to their anti-radical and reducing power activities. The treatment of mushrooms and avocado slices, with white cabbage, cauliflower, garlic and scallion extracts, reduced color changes during storage at 4 °C and -18 °C. Storage temperature and the type of extract employed influenced change of color variables. The contribution of polyphenols on measured antioxidant activity of extracts was also discussed. Allium antibrowning properties were closely related to antioxidant capacity, while the Brassica extracts were less effective. Treatment with Allium extracts extended the storage time of frozen and refrigerated mushrooms and avocado slices, in comparison with untreated samples.

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The relationship between antibrowning, anti-radical and reducing capacity of Brassica and Allium extracts

International Journal of Food Studies ◽

10.7455/ijfs/3.1.2014.a8 ◽

2014 ◽

Vol 3 (1) ◽

Cited By ~ 2

Author(s):

Mariela C Bustos ◽

Lina Marcela Agudelo-Laverde ◽

Florencia Mazzobre ◽

Pilar Buera

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Storage Time ◽

Storage Temperature ◽

Reducing Power ◽

Color Changes ◽

C Storage ◽

White Cabbage ◽

The Relationship ◽

Reducing Capacity

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Effects of Storage Time and Temperature on Antioxidants in Juice from Momordica charantia L. and Momordica charantia L. var. abbreviata Ser.

Molecules ◽

10.3390/molecules25163614 ◽

2020 ◽

Vol 25 (16) ◽

pp. 3614

Author(s):

Yung-Sheng Lin ◽

Wen-Ying Huang ◽

Pang-Yen Ho ◽

Shiou-Yih Hu ◽

Ying-Yi Lin ◽

...

Keyword(s):

Antioxidant Activity ◽

Storage Time ◽

Storage Temperature ◽

Antioxidant Activities ◽

Reducing Power ◽

Dry Weight ◽

Momordica Charantia ◽

Total Phenolic ◽

High Storage Temperature ◽

Dpph Scavenging

This study determined the antioxidant activities of juice from Momordica charantia L. (MC) and MC var. abbreviata Ser. (MCVAS) by analyzing 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, ferric reducing power (FRP), and total phenolic content (TPC). The effects of storage time and storage temperature on these antioxidant activities were investigated. Liquid chromatography–mass spectrometry was conducted to identify the major components of MC and MCVAS. The results revealed that the antioxidant activity of MCVAS was better than that of MC, possibly because of richer components of MCVAS. For MC and MCVAS, the scavenging concentrations of 50% DPPH were 3.33 and 1.19 mg/mL, respectively; moreover, the FRP values were 68.93 and 118.14 mg ascorbic acid equivalent/g dry weight, respectively; and the TPC values were 8.15 and 11.47 mg gallic acid equivalent/g dry weight, respectively. The antioxidant activities of MC and MCVAS decreased with storage time. High storage temperature decreased antioxidant activity more quickly than a low temperature. In addition, MC had exhibited a faster decline in DPPH scavenging ability and FRP than MCVAS during 24-day storage, but no difference was observed in TPC.

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PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i6.32119 ◽

2019 ◽

pp. 276-279 ◽

Cited By ~ 1

Author(s):

ABDELFATTAH EL MOUSSAOUI ◽

FATIMA ZAHRA JAWHARI ◽

DALILA BOUSTA ◽

AMINA BARI

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Antioxidant Capacity ◽

Radical Scavenging ◽

Reducing Power ◽

Mineral Elements ◽

Free Radical Scavenging ◽

Butylated Hydroxytoluene ◽

Phytochemical Analysis ◽

Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

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Evaluation of Antioxidant and Antibacterial Properties of Magnolia champaca L (Magnoliaceae) Stem Bark Extract

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v23i2.48328 ◽

2020 ◽

Vol 23 (2) ◽

pp. 96-102

Author(s):

Md Mahadi Hasan ◽

Masuma Akter ◽

Md Ekramul Islam ◽

Md Aziz Abdur Rahman ◽

Mst Shahnaj Parvin

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antibacterial Properties ◽

Reducing Power ◽

Stem Bark ◽

Total Antioxidant ◽

Pharmacologically Active

The current study was designated to explore the antioxidant and antibacterial properties of the methanolic extract of Magnolia champaca stem bark and its different fractions. Antioxidant activity was assessed using total antioxidant capacity, ferric reducing power, DPPH, hydroxyl and hydrogen peroxide scavenging assay. Antibacterial activity was evaluated against five gram positive and five gram negative bacteria using disc diffusion assay method. Among the different fractions, chloroform fraction (CHF) and ethyl acetate fraction (EAF) showed the highest antioxidant activity whereas aqueous fraction (AQF) showed lowest activity in DPPH radical scavenging assay with IC50 of 12.12, 22.41 and 55.16 μg/ml, respectively. Both of the extracts CHF and EAF also exhibited highest total antioxidant capacity, ferric reducing power and hydrogen peroxide scavenging activity with concentration dependent manner when compared to standard BHT. Moderate to potent antibacterial activity was observed against all tested organisms compared to standard azithromycin. The results from the present study revealed that the different fractions of stem bark of M. champaca specially CHF and EAF possess antioxidant and antibacterial property which support its use in traditional medicine and suggesting that the plant may be further investigated to discover its pharmacologically active natural products. Bangladesh Pharmaceutical Journal 23(2): 96-102, 2020

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Antioxidant Activity of Metabolites from Coleonema Album (Rutaceae)

Natural Product Communications ◽

10.1177/1934578x0600100505 ◽

2006 ◽

Vol 1 (5) ◽

pp. 1934578X0600100 ◽

Cited By ~ 2

Author(s):

Lindy L. Esterhuizen ◽

Riaan Meyer ◽

Ian A. Dubery

Keyword(s):

Antioxidant Activity ◽

South African ◽

Structural Information ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Active Components ◽

Bioassay Guided Fractionation ◽

The Relationship

Coleonema album, a member of the South African ‘Fynbos’ biome, was evaluated for its antioxidant and free radical scavenging activity. Ethanol- and acetone-based extracts from plant material obtained from two different geographical areas were analysed. A bioassay-guided fractionation methodology was followed for screening of active compounds. The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-TLC method revealed the presence of a number of antioxidants which were quantified by the DPPH-spectrophotometric assay and the oxygen radical absorbance capacity (ORAC) assay. The C. album extracts possessed significant in vitro antioxidant activity, a large portion of which appeared to be contributed by the phenolic compounds. In contrast, the reducing power of the extracts could not be correlated with the observed antioxidant activity. Identification and structural information of the active components were obtained by a combination of preparative TLC and LC-MS which revealed the presence of coumarin aglycones and glycosides. The results of this study indicate that C. album contains strong antioxidants that warrant further investigation into the relationship between the structure and activity of the active coumarin metabolites.

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Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe3O4 Nanoparticles by the Ugi Multicomponent Reaction

Molecules ◽

10.3390/molecules26195726 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5726

Author(s):

Yarelys Elena Augusto-Jimenez ◽

Marcela González-Montoya ◽

Dany Naranjo-Feliciano ◽

Daniel Uribe-Ramírez ◽

Eliseo Cristiani-Urbina ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Magnetite Nanoparticles ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Bioactive Peptide ◽

Peptide Fraction ◽

Scanning Transmission ◽

Peptide Fractions

The conjugation of biomolecules to magnetic nanoparticles has emerged as promising approach in biomedicine as the treatment of several diseases, such as cancer. In this study, conjugation of bioactive peptide fractions from germinated soybeans to magnetite nanoparticles was achieved. Different fractions of germinated soybean peptides (>10 kDa and 5–10 kDa) were for the first time conjugated to previously coated magnetite nanoparticles (with 3-aminopropyltriethoxysilane (APTES) and sodium citrate) by the Ugi four-component reaction. The crystallinity of the nanoparticles was corroborated by X-ray diffraction, while the particle size was determined by scanning transmission electron microscopy. The analyses were carried out using infrared and ultraviolet–visible spectroscopy, dynamic light scattering, and thermogravimetry, which confirmed the coating and functionalization of the magnetite nanoparticles and conjugation of different peptide fractions on their surfaces. The antioxidant activity of the conjugates was determined by the reducing power and hydroxyl radical scavenging activity. The nanoparticles synthesized represent promising materials, as they have found applications in bionanotechnology for enhanced treatment of diseases, such as cancer, due to a higher antioxidant capacity than that of fractions without conjugation. The highest antioxidant capacity was observed for a >10 kDa peptide fraction conjugated to the magnetite nanoparticles coated with APTES.

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Impact of refrigerated storage on the bioactive compounds and antioxidant capacity of two Algerian carrot varieties (Daucus carota L.)

Acta Universitatis Sapientiae Alimentaria ◽

10.2478/ausal-2020-0001 ◽

2020 ◽

Vol 13 (1) ◽

pp. 5-31

Author(s):

L. Arkoub-Djermoune ◽

H. Louaileche ◽

F. Benmeziane ◽

K. Madani ◽

L. Boulekbache-Makhlouf

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Bioactive Compounds ◽

Daucus Carota ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Bioactive Compound ◽

Total Phenolic ◽

Daucus Carota L

Abstract Carrot (Daucus carota L.) is one of the main root vegetables rich in bioactive compounds with appreciable health-promoting properties, largely consumed in Algeria. In the current study, the storage effect (at 4 °C throughout 12 days) on bioactive compound stability and the antioxidant activity of two Algerian orange carrot varieties (Supermuscade and Touchon) were investigated. Total phenolic content of samples was determined by the Folin–Ciocâlteu method. Antioxidant capacity was determined spectrophotometrically, based on the evaluation of Free Radical Scavenging Activity (FRSA) using DPPH radical and Ferric Reducing Power (FRP). The results showed that the Touchon variety is richer in phenolics, flavonoids, and carotenoids and presents higher antioxidant activity in comparison with the Supermuscade variety. At the end of storage, the bioactive compound content and antiradical activity increased significantly (p < 0.05). Also, an extremely significant correlation (p < 0.001) was observed between the antioxidant contents and the antioxidant capacities of aqueous carrot extracts.

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Serangan Sitophilus oryzae Pada Beras Dari Beberapa Varietas Padi dan Suhu Penyimpanan

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v22i1.34672 ◽

2020 ◽

Vol 22 (1) ◽

pp. 16

Author(s):

Rizma Dwi Mastuti ◽

Subagiya Subagiya ◽

Retno Wijayanti

Keyword(s):

Attack Rate ◽

Storage Time ◽

Plant Disease ◽

Yield Loss ◽

Storage Temperature ◽

Sitophilus Oryzae ◽

Brown Rice ◽

Black Rice ◽

C Storage ◽

Storage Temperatures

Post-harvest with storage are important to maintain the supply of rice. One of the obstacles found in storing rice is the attack of Sitophilus oryzae which is responsible of causing yield loss. The attack of S. oryzae on rice can be caused by storage temperature and protein content on rice. The objective of this research was to determine the attack rate of S.oryzae in several paddy varieties and different storage temperatures. The research was conducted in Laboratory of Pest and Plant Disease Faculty of Agriculture Universitas Sebelas Maret from March-June 2019. The method used was Nested Design with 2 factors and 3 replications. The treatments given were storage temperature (29oC, 39oC, 49oC and 59oC) and paddy varieties (brown rice, black rice, Rojolele and IR64). Observation variable were number of imago, pupae, larvae, percentage of decrease in rice weight, broken rice and rice powder. The results showed that S. oryzae was able to survive at a storage temperature of 29oC. Storage temperatures which increased by more than 29°C causing mortality of S.oryzae up to 100%. The longer storage time will cause an increase in population and S.oryzae attack rate.

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Influence of Storage Conditions and Preservatives on Metabolite Fingerprints in Urine

Metabolites ◽

10.3390/metabo9100203 ◽

2019 ◽

Vol 9 (10) ◽

pp. 203 ◽

Cited By ~ 4

Author(s):

Xinchen Wang ◽

Haiwei Gu ◽

Susana A. Palma-Duran ◽

Andres Fierro ◽

Paniz Jasbi ◽

...

Keyword(s):

Large Scale ◽

Storage Time ◽

Storage Temperature ◽

Urinary Metabolites ◽

Storage Conditions ◽

Inhibitory Effect ◽

Urine Samples ◽

C Storage ◽

Liquid Chromatography Tandem Mass ◽

The Stability

Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.

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Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

Antioxidants ◽

10.3390/antiox9010076 ◽

2020 ◽

Vol 9 (1) ◽

pp. 76 ◽

Cited By ~ 3

Author(s):

Natividad Chaves ◽

Antonio Santiago ◽

Juan Carlos Alías

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Plant Species ◽

Radical Scavenging ◽

Reducing Power ◽

Trolox Equivalent Antioxidant Capacity ◽

Antioxidant Compounds ◽

Frap Assay ◽

Trolox Equivalent

Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the highest antioxidant activity. The aim of this work was to verify whether different methods show the same sensitivity and/or capacity to discriminate the antioxidant activity of the extract of different plant species. To that end, we selected 12 species with different content of phenolic compounds. Their extracts were analyzed using the following methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, ferric reducing (FRAP) assay, Trolox equivalent antioxidant capacity (ABTS) assay, and reducing power (RP) assay. The four methods selected could quantify the antioxidant capacity of the 12 study species, although there were differences between them. The antioxidant activity values quantified through DPPH and RP were higher than the ones obtained by ABTS and FRAP, and these values varied among species. Thus, the hierarchization or categorization of these species was different depending on the method used. Another difference established between these methods was the sensitivity obtained with each of them. A cluster revealed that RP established the largest number of groups at the shortest distance from the root. Therefore, as it showed the best discrimination of differences and/or similarities between species, RP is considered in this study as the one with the highest sensitivity among the four studied methods. On the other hand, ABTS showed the lowest sensitivity. These results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter.

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