scholarly journals The functional analysis of ABCG transporters in the adaptation of pigeon pea (Cajanus cajan) to abiotic stresses

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10688
Author(s):  
Lili Niu ◽  
Hanghang Li ◽  
Zhihua Song ◽  
Biying Dong ◽  
Hongyan Cao ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Abiotic Stress ◽  
Stress Resistance ◽  
Abiotic Stresses ◽  
Expression Profiles ◽  
Complex Function ◽  
Pigeon Pea ◽  
Vital Role ◽  
Aluminum Stress ◽  
Transporter Family

ATP-binding cassette (ABC) transporters are a class of proteins found in living organisms that mediate transmembrane transport by hydrolyzing ATP. They play a vital role in the physiological processes of growth and development in plants. The most numerous sub-type transporter in the ABC transporter family is the ABCG group and which have the most complex function in a plant’s response to abiotic stresses. Our study focused on the effect of ABCG transporters in the adaptation of the pigeon pea to adverse environments (such as drought, salt, temperature, etc.). We conducted a functional analysis of ABCG transporters in the pigeon pea and their role in response to abiotic stresses. A total of 51 ABCG genes (CcABCGs) were identified, and phylogenetic analysis was conducted. We also identified the physicochemical properties of the encoded proteins, predicted their subcellular localization, and identified of the conserved domains. Expression analysis showed that ABCG genes have different expression profiles with tissues and abiotic stresses. Our results showed that CcABCG28 was up-regulated at low temperatures, and CcABCG7 was up-regulated with drought and aluminum stress. The initial results revealed that ABCG transporters are more effective in the abiotic stress resistance of pigeon peas, which improves our understanding of their application in abiotic stress resistance.

On farm abiotic stress management in pigeon pea (Cajanus cajan) and its impact on yield, economics and soil properties

2017 ◽  
Author(s):  
Y. P. Singh ◽  
Sudhir Singh ◽  
Anil Kumar Singh
Keyword(s):  
Abiotic Stress ◽  
Grain Yield ◽  
Short Duration ◽  
Abiotic Stresses ◽  
Seed Treatment ◽  
Nutrient Management ◽  
Pigeon Pea ◽  
Economic Benefits ◽  
Energy Productivity ◽  
Available N

The major abiotic stress limiting productivity of pigeon pea includes undulated topography, waterlogging, drought, frost, poor soil fertility. Management techniques of abiotic stresses significantly increased yield, net profit and B:C ratio as compared to farmers practice (FP). Adoption of abiotic stresses resulted in an increase of grain yield by 7.2 to 38.5% over FP. Major technological impact on grain yield compared to FP was in order: broad bed furrow (BBF) sowing method (38.5%) > nutrient management (21.0%) > seed treatment (14.9%) > short duration cultivar (7.3%) > precision land shaping (PLS) method (7.2%). Maximum additional cost on nutrient management was 2,360 ha-1 and it was minimum on seed treatment (265 ha-1), whereas saving due to BBF was .1,554 ha-1 and maximum additional net returns were obtained with BBF (30,551 ha-1) and minimum with PLS (4,804 ha-1) compared to FP. Maximum additional energy used was on nutrient management and minimum on seed treatment, whereas energy saved under BBF compared to FP. Higher additional net energy gain was with BBF followed by nutrient management, seed treatment, PLS and short duration cultivar over FP. PLS, BBF, seed treatment and nutrient management significantly increased infiltration rate and available N, P, K, S and Zn but decreased bulk density. Management of abiotic stresses by proper technologies increased pigeon pea production three times compared to average productivity of the country, resulted in increased economic benefits, energy productivity and improved soil physicochemical properties.

scholarly journals Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton

2016 ◽  
Vol 6 (12) ◽  
pp. 3951-3961 ◽  
Author(s):  
Weina Wang ◽  
Youlu Yuan ◽  
Can Yang ◽  
Shuaipeng Geng ◽  
Quan Sun ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Abiotic Stress ◽  
Verticillium Wilt ◽  
Abiotic Stresses ◽  
Homologous Sequence ◽  
Vascular Bundles ◽  
Virus Induced Gene Silencing ◽  
Biotic And Abiotic Stress ◽  
Biotic And Abiotic Stresses ◽  
Enhanced Resistance

Abstract Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants.

scholarly journals The Expected and Unexpected Roles of Nitrate Transporters in Plant Abiotic Stress Resistance and Their Regulation

2018 ◽  
Vol 19 (11) ◽  
pp. 3535 ◽  
Author(s):  
Guo-Bin Zhang ◽  
Shuan Meng ◽  
Ji-Ming Gong
Keyword(s):  
Abiotic Stress ◽  
Stress Resistance ◽  
Environmental Conditions ◽  
Abiotic Stresses ◽  
Nitrogen Availability ◽  
Nitrate Nitrogen ◽  
Adverse Environmental Conditions ◽  
Different Parts ◽  
Nitrate Transporters ◽  
Plant Abiotic Stress

Nitrate transporters are primarily responsible for absorption of nitrate from soil and nitrate translocation among different parts of plants. They deliver nitrate to where it is needed. However, recent studies have revealed that nitrate transporters are extensively involved in coping with adverse environmental conditions besides limited nitrate/nitrogen availability. In this review, we describe the functions of the nitrate transporters related to abiotic stresses and their regulation. The expected and unexpected roles of nitrate transporters in plant abiotic stress resistance will also be discussed.

scholarly journals DREB Genes from Common Bean (Phaseolus vulgaris L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity

2019 ◽  
Vol 2019 ◽  
pp. 1-28 ◽  
Author(s):  
Enéas Ricardo Konzen ◽  
Gustavo Henrique Recchia ◽  
Fernanda Cassieri ◽  
Danielle Gregorio Gomes Caldas ◽  
Jorge C. Berny Mier y Teran ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Amino Acid ◽  
Common Bean ◽  
Expression Profile ◽  
Abiotic Stresses ◽  
Nucleotide Diversity ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Nucleotide Variability ◽  
Amino Acid Diversity

We analyzed the nucleotide variability and the expression profile of DREB genes from common bean, a crop of high economic and nutritional value throughout the world but constantly affected by abiotic stresses in cultivation areas. As DREB genes have been constantly associated with abiotic stress tolerance, we systematically categorized 54 putative PvDREB genes distributed in the common bean genome. It involved from AP2 domain location and amino acid conservation analysis (valine at the 14th position) to the identification of conserved motifs within peptide sequences representing six subgroups (A-1 to A-6) of PvDREB proteins. Four genes (PvDREB1F, PvDREB2A, PvDREB5A, and PvDREB6B) were cloned and analyzed for their expression profiles under abiotic stresses and their nucleotide and amino acid diversity in genotypes of Andean and Mesoamerican origin, showing distinct patterns of expression and nucleotide variability. PvDREB1F and PvDREB5A showed high relative inducibilities when genotypes of common bean were submitted to stresses by drought, salt, cold, and ABA. PvDREB2A inducibility was predominantly localized to the stem under drought. PvDREB6B was previously described as an A-2 (DREB2) gene, but a detailed phylogenetic analysis and its expression profile clearly indicated it belongs to group A-6. PvDREB6B was found as a cold- and dehydration-responsive gene, mainly in leaves. Interestingly, PvDREB6B also showed a high nucleotide and amino acid diversity within its coding region, in comparison to the others, implicating in several nonsynonymous amino acid substitutions between Andean and Mesoamerican genotypes. The expression patterns and nucleotide diversity of each DREB found in this study revealed fundamental characteristics for further research aimed at understanding the molecular mechanisms associated with drought, salt, and cold tolerance in common bean, which could be performed based on association mapping and functional analyses.

scholarly journals Characterization of wheat homeodomain-leucine zipper family genes and functional analysis of TaHDZ5-6A in drought tolerance in transgenic Arabidopsis

2019 ◽  
Author(s):  
Shumin Li ◽  
Nan Chen ◽  
Fangfang Li ◽  
Fangming Mei ◽  
Zhongxue Wang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Expression Profiles ◽  
Wild Type ◽  
Protein Motifs ◽  
Zip Family ◽  
Zip Genes

Abstract Abstract Background: Many studies in Arabidopsis and rice have demonstrated that HD-Zip transcription factors play important roles in plant development and responses to abiotic stresses. Although common wheat (Triticum aestivum L.) is one of the most widely cultivated and consumed food crops in the world, the function of the HD-Zip proteins in wheat is still largely unknown. Results: To explore the potential biological functions of HD-Zip genes in wheat, we performed a bioinformatics and gene expression analysis of the HD-Zip family. We identified 113 HD-Zip members from wheat and classified them into four subfamilies (I-IV) based on phylogenic analysis against proteins from Arabidopsis, rice, and maize. Most HD-Zip genes are represented by two to three homeoalleles in wheat, which are named as TaHDZX_ZA, TaHDZX_ZB, or TaHDZX_ZD, where X denotes the gene number and Z the wheat chromosome on which it is located. TaHDZs in the same subfamily have similar protein motifs and intron/exon structures. The expression profiles of TaHDZ genes were analysed in different tissues, at different stages of vegetative growth, during seed development, and under drought stress. We found that most TaHDZ genes, especially those in subfamilies I and II, were induced by drought stress, suggesting the potential importance of subfamily I and II TaHDZ members in the responses to abiotic stress. Compared with wild-type (WT) plants, transgenic Arabidopsis plants overexpressing TaHDZ5-6A displayed enhanced drought tolerance, lower water loss rates, higher survival rates, and higher proline content under drought conditions. Additionally, the transcriptome analysis identified a number of differentially expressed genes between 35S::TaHDZ5-6A transgenic and wild-type plants, many of which are involved in stress response. Conclusions: Our results will facilitate further functional analysis of wheat HD-Zip genes, and also indicate that TaHDZ5-6A may participate in regulating the plant response to drought stress. Our experiments show that TaHDZ5-6A holds great potential for genetic improvement of abiotic stress tolerance in crops.

scholarly journals Characterization of wheat homeodomain-leucine zipper family genes and functional analysis of TaHDZ5-6A in drought tolerance in transgenic Arabidopsis

2020 ◽  
Author(s):  
Shumin Li ◽  
Nan Chen ◽  
Fangfang Li ◽  
Fangming Mei ◽  
Zhongxue Wang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Expression Profiles ◽  
Wild Type ◽  
Protein Motifs ◽  
Zip Family ◽  
Zip Genes

Abstract Background: Many studies in Arabidopsis and rice have demonstrated that HD-Zip transcription factors play important roles in plant development and responses to abiotic stresses. Although common wheat (Triticum aestivum L.) is one of the most widely cultivated and consumed food crops in the world, the function of the HD-Zip proteins in wheat is still largely unknown. Results: To explore the potential biological functions of HD-Zip genes in wheat, we performed a bioinformatics and gene expression analysis of the HD-Zip family. We identified 113 HD-Zip members from wheat and classified them into four subfamilies (I-IV) based on phylogenic analysis against proteins from Arabidopsis, rice, and maize. Most HD-Zip genes are represented by two to three homeoalleles in wheat, which are named as TaHDZX_ZA, TaHDZX_ZB, or TaHDZX_ZD, where X denotes the gene number and Z the wheat chromosome on which it is located. TaHDZs in the same subfamily have similar protein motifs and intron/exon structures. The expression profiles of TaHDZ genes were analysed in different tissues, at different stages of vegetative growth, during seed development, and under drought stress. We found that most TaHDZ genes, especially those in subfamilies I and II, were induced by drought stress, suggesting the potential importance of subfamily I and II TaHDZ members in the responses to abiotic stress. Compared with wild-type (WT) plants, transgenic Arabidopsis plants overexpressing TaHDZ5-6A displayed enhanced drought tolerance, lower water loss rates, higher survival rates, and higher proline content under drought conditions. Additionally, the transcriptome analysis identified a number of differentially expressed genes between 35S::TaHDZ5-6A transgenic and wild-type plants, many of which are involved in stress response. Conclusions: Our results will facilitate further functional analysis of wheat HD-Zip genes, and also indicate that TaHDZ5-6A may participate in regulating the plant response to drought stress. Our experiments show that TaHDZ5-6A holds great potential for genetic improvement of abiotic stress tolerance in crops.

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