scholarly journals The nutritional applications of garlic (Allium sativum) as natural feed additives in animals

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11934
Author(s):  
Jiashun Chen ◽  
Fang Wang ◽  
Yexin Yin ◽  
Xiaokang Ma
Keyword(s):  
Allium Sativum ◽  
Animal Feed ◽  
Animal Husbandry ◽  
Feed Additives ◽  
Bioactive Molecules ◽  
Herbal Remedies ◽  
Organosulfur Compounds ◽  
Beneficial Effects ◽  
Stress Markers

Garlic (Allium sativum) is an essential vegetable that has been widely utilized as seasoning, flavoring, culinary and in herbal remedies. Garlic contains several characteristic organosulfur compounds, such as diallyl sulfide, allicin (diallyl thiosulphate), γ-glutamylcysteine, and S-allyl cysteine (alliin) and ajoene, which garlic has beneficial effects on inflammation, oxidative stress markers, hypertension, hyperlipidaemia and endothelial function in vitro or in animal model. These bioactive molecules are also playing pivotal role in livestock and fisheries production apart from its application in humans. Supplementation of animal feed with garlic and its related products is consistent with the modern agricultural concept of organic animal husbandry. This review compiles the information describing the effects of feeding garlic and its extracts on selected performance parameters in animals (chicken, rabbits, ruminants, pigs and fish). This review may provide reference for scientists and entrepreneurs to investigate the applications of feeds added with garlic and allicin by-products for the improvement of animal husbandry and aquatic production.

scholarly journals In Vitro Evaluation of the Effects of Commercial Prebiotic GOS and FOS Products on Human Colonic Caco–2 Cells

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1281
Author(s):  
Geraldine M. Flaujac Lafontaine ◽  
Neville M. Fish ◽  
Ian F. Connerton
Keyword(s):  
Animal Feed ◽  
Feed Additives ◽  
Differentially Expressed ◽  
Control Group ◽  
Intestinal Epithelial ◽  
Beneficial Effects ◽  
Prebiotic Oligosaccharides ◽  
Energy Dependent ◽  
Colonic Cells

Prebiotic oligosaccharides are widely used as human and animal feed additives for their beneficial effects on the gut microbiota. However, there are limited data to assess the direct effect of such functional foods on the transcriptome of intestinal epithelial cells. The purpose of this study is to describe the differential transcriptomes and cellular pathways of colonic cells directly exposed to galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS). We have examined the differential gene expression of polarized Caco–2 cells treated with GOS or FOS products and their respective mock-treated cells using mRNA sequencing (RNA-seq). A total of 89 significant differentially expressed genes were identified between GOS and mock-treated groups. For FOS treatment, a reduced number of 12 significant genes were observed to be differentially expressed relative to the control group. KEGG and gene ontology functional analysis revealed that genes up-regulated in the presence of GOS were involved in digestion and absorption processes, fatty acids and steroids metabolism, potential antimicrobial proteins, energy-dependent and -independent transmembrane trafficking of solutes and amino acids. Using our data, we have established complementary non-prebiotic modes of action for these frequently used dietary fibers.

scholarly journals In Vitro Evaluation of the Effects of Prebiotics GOS and FOS on Human Colonic Caco–2 Cells

2020 ◽  
Author(s):  
Geraldine Lafontaine ◽  
Neville Fish ◽  
Ian Connerton
Keyword(s):  
Animal Feed ◽  
Feed Additives ◽  
Differentially Expressed ◽  
Control Group ◽  
Intestinal Epithelial ◽  
Beneficial Effects ◽  
Prebiotic Oligosaccharides ◽  
Energy Dependent ◽  
Colonic Cells

Prebiotic oligosaccharides are widely used as human and animal feed additives for their beneficial effects on the gut microbiota. However, there are limited data to assess the direct effect of such functional foods on the transcriptome of intestinal epithelial cells. The purpose of this study is to describe the differential transcriptomes and cellular pathways of colonic cells directly exposed to galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS). We have examined the differential gene expression of polarized Caco-2 cells treated with GOS or FOS and their respective mock-treated cells using mRNA sequencing (RNA-seq). A total of 89 significant differentially expressed genes were identified between GOS and mock-treated groups. For FOS treatment, a reduced number of 12 significant genes were observed to be differentially expressed relative to the control group. KEGG and Gene Ontology functional analysis revealed that genes up-regulated in the presence of GOS were involved in digestion and absorption processes, fatty acids and steroids metabolism, potential antimicrobial proteins, energy-dependent and -independent transmembrane trafficking of solutes and amino acids. Using our data, we have established complementary non-prebiotic modes of action for these frequently used dietary fibers.

scholarly journals IN VITRO ASSESSMENT OF BINDING CAPACITY OF COMBINED ADSORBENT (BENTONITE WITH YEAST CELL WALL EXTRACTS) AND AFLATOXIN B1

2020 ◽  
Vol 13 (1) ◽  
pp. 41-52
Author(s):  
Ksenija Nesic ◽  
Sandra Jaksic ◽  
Nenad Popov ◽  
Milica Zivkov-Balos ◽  
Marko Pajic ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Animal Feed ◽  
Binding Capacity ◽  
Animal Husbandry ◽  
Feed Additives ◽  
Point Of View ◽  
Feed Additive ◽  
European Regulation

The contamination of animal feed with mycotoxins is a worldwide problem in the animal husbandry, but it also represents a serious threat for the whole food chain. The health of both animals and humans is potentially endangered. From this point of view aflatoxins are a class of mycotoxins especially well known. Therefore, new strategies to combat these natural contaminants are constantly being developed. The most applied method to protect animals against aflatoxicosis is the utilization of feed additives aimed to adsorb aflatoxins. In order to estimate adsorbing potential of feed additive “MycoStop DUPLO”, designed for the prevention and/or alleviation of adverse effects of aflatoxin B1 in animal nutrition, in vitro trial was conducted. As a result of the experiment, conducted at pH 5 during 120 minutes of incubation at 37°C, the optimal formulation of the adsorbent was revealed. This product, in low concentration and in the presence of high amounts of toxin, met the stringent European regulation requirements for minimum 90% aflatoxin binding efficiency (90.1% achieved with 0.02% adsorbent and 4 mg/L toxin concentration). In higher adsorbent (0.2%), and lower toxin (0.2 mg/L) conditions, adsorption was 99.6%. Such outcome indicated the validity of in vitro experimental approach which can serve as a reliable fast tool for triage of adsorbents and preselect them for in vivo tests.

scholarly journals Effects of Xylo-Oligosaccharides on Broiler Chicken Performance and Microbiota

2015 ◽  
Vol 81 (17) ◽  
pp. 5880-5888 ◽  
Author(s):  
C. De Maesschalck ◽  
V. Eeckhaut ◽  
L. Maertens ◽  
L. De Lange ◽  
L. Marchal ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Degradation Products ◽  
Hydrolytic Degradation ◽  
Feed Additives ◽  
Gut Health ◽  
Feed Conversion ◽  
Content Type ◽  
Beneficial Effects ◽  
Coa Transferase

ABSTRACTIn broiler chickens, feed additives, including prebiotics, are widely used to improve gut health and to stimulate performance. Xylo-oligosaccharides (XOS) are hydrolytic degradation products of arabinoxylans that can be fermented by the gut microbiota. In the current study, we aimed to analyze the prebiotic properties of XOS when added to the broiler diet. Administration of XOS to chickens, in addition to a wheat-rye-based diet, significantly improved the feed conversion ratio. XOS significantly increased villus length in the ileum. It also significantly increased numbers of lactobacilli in the colon andClostridiumcluster XIVa in the ceca. Moreover, the number of gene copies encoding the key bacterial enzyme for butyrate production, butyryl-coenzyme A (butyryl-CoA):acetate CoA transferase, was significantly increased in the ceca of chickens administered XOS. In this group of chickens, at the species level,Lactobacillus crispatusandAnaerostipes butyraticuswere significantly increased in abundance in the colon and cecum, respectively.In vitrofermentation of XOS revealed cross-feeding betweenL. crispatusandA. butyraticus. Lactate, produced byL. crispatusduring XOS fermentation, was utilized by the butyrate-producingAnaerostipesspecies. These data show the beneficial effects of XOS on broiler performance when added to the feed, which potentially can be explained by stimulation of butyrate-producing bacteria through cross-feeding of lactate and subsequent effects of butyrate on gastrointestinal function.

scholarly journals In vitro determination of inhibitory effects by humic substances complexing Zn and Se on SARS-CoV-2 virus replication

2021 ◽  
Author(s):  
Bernadett Palyi ◽  
Zoltan Kis ◽  
Polett Hajdrik ◽  
Noemi S Kovacs ◽  
Daniel S Veres ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antiviral Activity ◽  
Humic Substances ◽  
Animal Feed ◽  
Antiviral Effect ◽  
Nutritional Supplement ◽  
Feed Additives ◽  
Acid Complex ◽  
Alpha Variant

Humic substances are well known human nutritional supplement materials and play important performance-enhancing roles as animal feed additives, too. For decades, ingredients of humic substances have also been proven to carry potent antiviral effects against different viruses. Here, the antiviral activity of a humic substance containing ascorbic acid, Se- and Zn2+ ions intended as a nutritional supplement material was investigated against SARS-CoV-2 virus B1.1.7 Variant of Concern (Alpha Variant) in a VeroE6 cell line. Results show that this combination has a significant in vitro antiviral effect at a very low concentration range of its intended active ingredients. Even picomolar concentration ranges of humic substances, vitamin C and Zn/Se ions in the given composition were enough to achieve fifty percent viral replication inhibition in the applied SARS-CoV-2 virus inhibition test. Keywords: SARS-CoV-2, humic acid, fulvic acid, Zn-Se-ascorbic acid complex, antiviral activity, RT-PCR

scholarly journals Analysis of Policy Making Factors on The Prohibition of Hormones and Antibiotics Use for Feed as a Public Health Protection

2017 ◽  
Vol 2 (2) ◽  
pp. 14
Author(s):  
Zaima Amalia ◽  
Wiku Adisasmito
Keyword(s):  
Local Governments ◽  
Animal Feed ◽  
Animal Husbandry ◽  
Antibiotic Use ◽  
Feed Additives ◽  
Growth Promoters ◽  
Antibiotics Use ◽  
Emerging Issues ◽  
The Government ◽  
Public Health Protection

Food safety is one of the current international emerging issues. The danger of using antibiotics in animal husbandry is one of the contributors to human resistance. In Indonesia, antibiotics are commonly used as growth promoters in animal husbandry. Prohibition on the use of hormones and antibiotics mixed into animal feed written in Law No. 18 of 2009 on Animal Husbandry and Health which is then clarified by the Minister of Agriculture Regulation No. 14/Permentan/PK.350/5/2017 on the Classification of Animal Drugs. The purpose of this study is to determine the factors which influence the formation of policy on the prohibition of hormone and antibiotic use as feed additives, especially health, legal, political and economic factors. This research uses descriptive study by qualitative approach. Data were collected using in-depth interviews and literature studies. The result shows that legal factors have a stronger influence than health, economic and political factors. The study recommends that the government should develop monitoring programs, conduct surveillance in feed and breeder entrepreneurs, establish good coordination with local governments, conduct further residue studies on animal source food, and further review the economic impact of the policy. Breeders also need to improve the cleanliness of the cage and try natural feed. While feed entrepreneurs need to find a replacement of antibiotic/hormone mixed feed safely.

scholarly journals Effects of Olive (Olea europaea L.) Leaves with Antioxidant and Antimicrobial Activities on In Vitro Ruminal Fermentation and Methane Emission

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2008
Author(s):  
Shin Ja Lee ◽  
Hyun Sang Kim ◽  
Jun Sik Eom ◽  
You Young Choi ◽  
Seong Uk Jo ◽  
...  
Keyword(s):  
Animal Feed ◽  
Basal Diet ◽  
Antimicrobial Activities ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Ruminal Fermentation ◽  
Prevotella Ruminicola ◽  
Degrading Bacteria

We evaluated whether olive leaves (OLs) are effective as feed additives and supplements for ruminants and the potential methane reduction effects during in vitro fermentation. Two Hanwoo cows (460 ± 20 kg) equipped with cannula were fed Timothy hay and corn-based feed 3% of the body weight at a ratio of 6:4 (8:30 a.m. and 5:00 p.m.). Ruminal fluid from the cows was collected and mixed before morning feeding. In vitro batch fermentation was monitored after 12 and 24 h of incubation at 39 °C, and OLs were used as supplements to achieve the concentration of 5% in the basal diet. At 12 h of fermentation, methane production decreased in the 5% OLs group compared to that in the control group, but not at 24 h. The proportion of cellulose-degrading bacteria, Fibrobacter succinogenes, Ruminococcus albus, and Ruminococcus flavefaciens, tended to increase in the 5% OLs group at 12 h. The amount of ammonia produced was the same as the polymerase chain reaction result for Prevotella ruminicola. At 12 h, the proportion of Prevotella ruminicola was significantly higher in the 5% OLs group. OLs may be used incorporated with protein byproducts or other methane-reducing agents in animal feed.

scholarly journals Beneficial Effects of Organosulfur Compounds from Allium cepa on Gut Health: A Systematic Review

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1680
Author(s):  
Enrique Guillamón ◽  
Pedro Andreo-Martínez ◽  
Nuria Mut-Salud ◽  
Juristo Fonollá ◽  
Alberto Baños
Keyword(s):  
Systematic Review ◽  
Allium Cepa ◽  
Dietary Intervention ◽  
Biological Properties ◽  
Organosulfur Compounds ◽  
Gut Health ◽  
Intestinal Health ◽  
Beneficial Effects

Dietary changes affect the composition and structure of gut microbiota (GM) in animals and humans. One of the beneficial effects of consuming products derived from plants is the positive influence on immunity and gastrointestinal health. Species belonging to the genus Allium contain many organosulfur compounds (OSCs) that have been widely studied showing their biological properties and beneficial effects on intestinal health and GM. This is the first systematic review of OSCs from Allium performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and it is based on the evidence that we found in literature about the benefits on the GM and intestinal health demonstrated by OSCs from Allium, and specifically from onion. OSCs from Allium cepa have shown a significant antibacterial activity against a broad spectrum of antibiotic-resistant Gram-positive and Gram-negative bacteria. In addition, the intake of OSCs from onion was able to modulate the composition of GM, increasing the beneficial bacterial populations in animal models. Moreover, the beneficial effects observed in murine models of colitis suggest that these compounds could be suitable candidates for the treatment of inflammatory bowel disease (IBD) or reverse the dysbiosis caused by a high-fat diet (HFD). Despite the evidence found both in vitro and in vivo, we have not found any article that tested OSCs different from allicin in clinical trials or dietary intervention studies in humans. In this sense, it would be interesting to conduct new research that tests the benefits of these compounds in human GM.

scholarly journals Phytogenic Bioactive Compounds Shape Fish Mucosal Immunity

2021 ◽  
Vol 12 ◽  
Author(s):  
Joana P. Firmino ◽  
Jorge Galindo-Villegas ◽  
Felipe E. Reyes-López ◽  
Enric Gisbert
Keyword(s):  
Mucosal Immunity ◽  
Mode Of Action ◽  
Bacterial Resistance ◽  
Wide Spectrum ◽  
Feed Additives ◽  
Mucosal Barrier ◽  
Reproducible Research ◽  
Organosulfur Compounds ◽  
The Impact

Aquaculture growth will unavoidably involve the implementation of innovative and sustainable production strategies, being functional feeds among the most promising ones. A wide spectrum of phytogenics, particularly those containing terpenes and organosulfur compounds, are increasingly studied in aquafeeds, due to their growth promoting, antimicrobial, immunostimulant, antioxidant, anti-inflammatory and sedative properties. This trend relies on the importance of the mucosal barrier in the fish defense. Establishing the phytogenics’ mode of action in mucosal tissues is of importance for further use and safe administration. Although the impact of phytogenics upon fish mucosal immunity has been extensively approached, most of the studies fail in addressing the mechanisms underlying their pharmacological effects. Unstandardized testing as an extended practice also questions the reproducibility and safety of such studies, limiting the use of phytogenics at commercial scale. The information presented herein provides insight on the fish mucosal immune responses to phytogenics, suggesting their mode of action, and ultimately encouraging the practice of reliable and reproducible research for novel feed additives for aquafeeds. For proper screening, characterization and optimization of their mode of action, we encourage the evaluation of purified compounds using in vitro systems before moving forward to in vivo trials. The formulation of additives with combinations of compounds previously characterized is recommended to avoid bacterial resistance. To improve the delivery of phytogenics and overcome limitations associated to compounds volatility and susceptibility to degradation, the use of encapsulation is advisable. Besides, newer approaches and dedicated methodologies are needed to elucidate the phytogenics pharmacokinetics and mode of action in depth.

Developments in techniques to test the efficacy of animal feed products

2021 ◽  
pp. 125-150
Author(s):  
Gerhard Flachowsky ◽  
◽  
Ulrich Meyer ◽  
Keyword(s):  
Animal Feed ◽  
Animal Health ◽  
Feed Additives ◽  
In Vivo Studies ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Key Issues ◽  
Key Steps

Various animal feed products may influence animal health, conversion of animal feed into food of animal origin and the emissions caused by animals. All these matters are regulated in the directives of the European Food Safety Authority (EFSA). This chapter first discusses EFSA guidance on how to compile dossiers for feed additives. The chapter then discusses key issues and steps in demonstrating the efficacy of new animal feed products: reduction of nitrogen (N) excretion, reduction of feed contamination of by mycotoxins and, finally, reduction of methane emissions with feed additives. Key steps, such as the use of in vitro and in vivo studies, to test the effects of feed additives are discussed in detail.

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