Study of the Variation of Some Biochemical Parameters in Rattus norgevicus

The biochemical assessment in animals is of capital interest to define the diagnosis of many diseases. The variation of biochemical parameters (urea, glycemia, creatinemia, transaminases: GPT and GOT, total cholesterol, HDL-c, LDL-c, triglycerides and total lipids) is an indicator of the state of health of certain vital organs such as the kidney, liver, pancreas, veins, heart, etc... . For this study, to be able to determine the normal values of the rats, a set of batches of male and female Wistar rats was formed and acclimatized for 2 months. Each batch set consisted of thirty-nine (39) rats. Each rat was weighed and then sampled by venipuncture at the caudal level to collect their blood in a dry tube (red). The red tube was used to determine the biochemical parameters. The results of this study showed that some animals already had certain pathologies before the use of any beneficial or harmful substance on them. However, most of the animals used have normal biochemical parameters revealing the good state of their various vital functions (kidney, liver, heart, veins, pancreas, etc.). These values of determining standard biochemical parameters can form a basis for future tests.

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The Interactive Effect of High Doses of Chromium(III) and Different Iron(III) Levels on the Carbohydrate Status, Lipid Profile, and Selected Biochemical Parameters in Female Wistar Rats

Nutrients ◽

10.3390/nu12103070 ◽

2020 ◽

Vol 12 (10) ◽

pp. 3070

Author(s):

Halina Zofia Staniek ◽

Ewelina Król ◽

Rafał Wojciech Wójciak

Keyword(s):

Insulin Resistance ◽

Lipid Profile ◽

Carbohydrate Metabolism ◽

Biochemical Parameters ◽

Wistar Rats ◽

Interactive Effect ◽

The Other ◽

Iron Deficient ◽

Female Wistar Rats ◽

High Doses

The aim of the study was to evaluate the main and interactive effects of chromium(III) propionate complex (Cr3) supplementation and different iron supply on the carbohydrate metabolism, lipid profile and other selected biochemical parameters of rats. The experiment was carried out in a two-factor design, in which rats were fed a diet with different proportions of Fe(III) and Cr(III) for six weeks. Fifty-four healthy female Wistar rats were divided into nine experimental groups with different Fe(III) levels, i.e. adequate—control group (45 mg/kg)—100% recommended daily dietary dose of Fe for rodents, deficient (5 mg/kg) and oversupply (180 mg/kg—400%). At the same time they were supplemented with Cr(III) of doses 1 (adequate), 50 and 500 mg/kg of diet. The activity and concentrations of most biochemical parameters were measured with standard enzymatic, kinetic, and colorimetric methods. HOMA-IR and QUICKI indexes were calculated according to appropriate formulas. It was found that there was an interactive effect of high Cr(III) doses and different Fe(III) levels in the diet on the carbohydrate metabolism and insulin resistance indexes. The presented results suggested that iron deficient diet fed animals led to insulin resistance; however, an effect is attenuated by Cr(III) supplementation at high doses. There were no significant changes in the rats’ lipid profile (except for the high density lipoprotein cholesterol (HDL-C) level) and most of the other biochemical parameters, such as the leptin, aspartate aminotransferase (AST), alanine transaminase (ALT), total protein (TP), creatinine (Crea) and the urea (BUN) concentrations. The study proved that the Cr(III) supplementation, independently and in combination with diversified Fe(III) content in the diet, affected the carbohydrate metabolism and insulin resistance indexes but did not affect lipid profile and most of the other biochemical parameters in healthy rats. The findings proved the role of Fe and Cr(III) and their interactions on disturbances carbohydrates metabolism.

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NILAI RUJUKAN UNTUK EVALUASI FUNGSI HATI DAN GINJAL PADA TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

Jurnal Pendidikan Matematika dan IPA ◽

10.26418/jpmipa.v10i2.34144 ◽

2019 ◽

Vol 10 (2) ◽

pp. 81

Author(s):

Laksmindra Fitria ◽

Fajar Lukitowati ◽

Dian Kristiawati

Keyword(s):

Reference Values ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Normal Values ◽

Local Population ◽

Renal Functions ◽

Metabolic Functions ◽

Foreign Countries ◽

Wide Range ◽

Female Wistar Rats

Evaluation of liver and renal functions are routine tests performed in preclinical studies. Liver plays a role in detoxification and secretion of various substances to regulate a wide range of metabolic functions. Kidneys serves as the main excretory and osmoregulatory organs. Thus, assessment of liver and renal functions are important in identifying the presence of damages and diseases as well as monitoring body responses towards treatments or therapy. Therefore, normal values should be established for valid reference. Until now, many researchers still use references from various publications, including foreign countries with different environmental factors and method of animals care. This study was aimed to provide reference values for liver and renal functions in male and female Wistar rats. Animals were sourced from LPPT-UGM. Age 4-, 6-, and 8-week-old represent juvenile, preadult, and adult. Blood samples were collected from orbital sinus of anesthetized animals. Sera were tested for ALT, AST, BUN, and creatinine based on spectrophotometry. Results demonstrated that values of most parameters varied, depend on sex and age. According to this finding, it can be concluded that reference values cannot be generalized but must be determined by sex and age in local population.

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Influence of treatment with quercetin on lipid parameters and oxidative stress of pregnant diabetic rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2012-0173 ◽

2013 ◽

Vol 91 (2) ◽

pp. 171-177 ◽

Cited By ~ 14

Author(s):

Camila Pereira Braga ◽

Ana Carolina Momentti ◽

Fernando Barbosa Peixoto ◽

Rafaela de Fátima Ferreira Baptista ◽

Felipe André dos Santos ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Biochemical Parameters ◽

Wistar Rats ◽

Diabetic Rats ◽

Lipid Hydroperoxides ◽

Female Wistar Rats ◽

Lipid Parameters ◽

And Oxidative Stress ◽

Pregnant Diabetic

Among the numerous coadjuvant therapies that could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of quercetin on biochemical parameters in streptozotocin-induced (60 mg/kg body mass, by intraperitoneal injection) diabetic rats. A total of 32 female Wistar rats were distributed among 4 groups as follows: control (G1); control treated with quercetin (G2); diabetic (G3); and diabetic treated with quercetin (G4). Quercetin administered to pregnant diabetic rats controlled dyslipidemia and improved lipid profiles in diabetes mellitus, regulated oxidative stress by reducing the generation of lipid hydroperoxides, and increased the activity of the antioxidant enzyme glutathione peroxidase.

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Effect of cholesterol and fructose supplementation on the biochemical parameters, hormonal, oxidative stress and histopathology of the abdominal aorta of young male and female Wistar rats in growth

Archives of Cardiovascular Diseases Supplements ◽

10.1016/j.acvdsp.2019.05.024 ◽

2019 ◽

Vol 11 (3) ◽

pp. e341-e342

Author(s):

Lynda Aïnouz-Ammar Aouchiche ◽

Lokman Kechekoul ◽

Sofiane Boudjellaba ◽

Djamila Benaziza ◽

Kahina Chabane ◽

...

Keyword(s):

Oxidative Stress ◽

Abdominal Aorta ◽

Biochemical Parameters ◽

Wistar Rats ◽

Young Male ◽

Male And Female ◽

Female Wistar Rats

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Valuation of three different anaesthetic protocols on complete blood count and biochemical parameters on Wistar rats

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.16057 ◽

2018 ◽

Vol 68 (4) ◽

pp. 587 ◽

Cited By ~ 1

Author(s):

P. P. LELOVAS ◽

M. S. STASINOPOULOU ◽

E. G. BALAFAS ◽

M. A. NIKITA ◽

N. T. SISKOS ◽

...

Keyword(s):

Biochemical Parameters ◽

Wistar Rats ◽

Blood Count ◽

Complete Blood Count ◽

Vena Cava ◽

Histological Evaluation ◽

Blood Collection ◽

Female Wistar Rats ◽

The Impact ◽

To Receive

The objective of the current study was to determine the impact of three different anaesthetic protocols on blood and biochemical parameters. Eighteen female Wistar rats (HsdOla:WI) 3 months old, weighting 197.09 ± 7.39 g were used. Baseline blood collection was performed in all animals from the lateral coccygeal vein for evaluation of glucose. The animals were then randomly allocated to receive one of three different anaesthetic protocols: dexmedetomidine/ketamine intramuscularly (0.25 mg/kg and 50 mg/kg respectively), or isoflurane 0.2 ml on cotton inside a syringe case, or isoflurane administered by vaporiser (5% induction and 2-3% maintenance of anaesthesia, delivered in oxygen flow 1 L/min). Blood samples were collected from caudal vena cava for complete blood count and biochemical analysis, while the lung and the liver were harvested for histological evaluation. Comparison between groups, as far as complete blood count parameters is concerned, revealed statistical significant differences in red blood cells, haematocrit and haemoglobin values with lower values being observed in vaporiser anaesthesia group. Furthermore, within group comparison revealed statistical significant differences for glucose in all three groups. No evidence of inflammatory, degenerative neoplastic or toxicity related lesions were observed during histological evaluation of the lung and liver.

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Endothelial, renal and hepatic variables in wistar rats treated with Vancomycin

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201420140204 ◽

2014 ◽

Vol 86 (4) ◽

pp. 1963-1972 ◽

Cited By ~ 4

Author(s):

FELIPE R. BRUNIERA ◽

FELIPE M. FERREIRA ◽

LUIZ R.M. SAVIOLI ◽

MARCELO R. BACCI ◽

DAVID FEDER ◽

...

Keyword(s):

Biochemical Parameters ◽

Wistar Rats ◽

Femoral Vein ◽

Beta Carotene ◽

Alpha Tocopherol ◽

Histopathological Analysis ◽

First Choice ◽

Liver And Kidney ◽

Hs Crp ◽

Kidney Liver

Vancomycin (VCM) is indicated in combat against Gram-positive infections, but it is not considered a first-choice drug because of its adverse effects. It is believed that oxidative stress is the primary mechanism of endothelial injury and the consequent VCM toxicity, which varies from phlebitis to nephrotoxicity. Moreover, dose recommendations, dilution, rates and types of infusion are still controversial. The aim of this study was to determine the effect of different VCM dilutions in endothelial, liver and kidney injuries by biochemical parameters and histopathological analysis. Wistar rats were randomly divided into six groups and subjected to femoral vein cannulation for drug administration. Control groups received 0.9 ml of saline and the others received VCM (10mg/Kg/day) at dilutions of 5.0 and 10.0 mg/mL for 3 and 7 days. Homocysteine, hs-CRP, AST, ALT, GGT, urea, creatinine, lycopene, alpha-tocopherol, beta-carotene and retinol were analyzed. Kidney, liver and cannulated femoral vein fragments were collected.This study showed alterations in ALT which featured hepatotoxicity. However, drug dilutions were not able to show changes in other biochemical parameters. In contrast, kidney and endothelium pathological changes were observed. More studies are needed to characterize VCM induced kidney and endothelium toxicity and biochemical markers able to show such morphological modifications.

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ANTI-ESTROGENIC PROPERTIES OF ETHANOLIC LEAF EXTRACT OF BOERHAVIA DIFFUSA ON FEMALE WISTAR RATS.

10.1055/s-0037-1608549 ◽

2017 ◽

Author(s):

C Olajide Omolola ◽

J Okpuzor ◽

I Nnadozie Jane ◽

R Adewole Adeniyi

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Female Wistar Rats ◽

Estrogenic Properties ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

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NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0750569 ◽

1974 ◽

Vol 75 (3) ◽

pp. 569-578 ◽

Cited By ~ 29

Author(s):

G. Buffler ◽

S. Roser

Keyword(s):

Wistar Rats ◽

Venous Blood ◽

Oestrous Cycle ◽

Female Rats ◽

Cycle Duration ◽

Follicular Growth ◽

The Third ◽

Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

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