scholarly journals Isolation and Molecular Characterisation of Vancomycin Resistant Bacteria Isolated from Selected Well Water in Ula-Ubie Community, Ahoada, Rivers State

2020 ◽  
pp. 70-77
Author(s):  
N. P. Akani ◽  
M. Okpokiri ◽  
R. R. Nrior
Keyword(s):  
Screening Method ◽  
Brain Heart Infusion ◽  
Molecular Methods ◽  
Nutrient Agar ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Well Water ◽  
Bacterial Isolates ◽  
Vancomycin Resistant ◽  
Rivers State

The resistance of bacteria to vancomycin has made the treatment of some infections more difficult to handle. The aim of this study was to isolate and molecularly characterize vancomycin resistant bacteria in selected well water with a view of determining the level of resistance in the environment. fifty (50) well water samples were collected from ten different stations in Ula-Ubie community, Ahoada, Rivers State for a period of six months (January 2019 to June, 2019). Bacteria diversity was analysed using standard microbiological techniques. In this technique, aliquots of 10-1, 10-2 and 10-3 dilutions were seeded into prepared Nutrient agar and Brain Heart infusion agar (BHI). Microbial loads were enumerated and distinct bacterial colonies were picked and inoculated on freshly prepared nutrient agar. Purification of isolates was done by streaking on freshly prepared nutrient agar plates until isolates were void of mixed culture. The isolates were further characterized using biochemical and molecular methods and preserved in bijou bottles containing 5 ml of 10% (v/v) glycerol. Isolates from the glycerol were subcultured on fresh nutrient agar plates before each use. The vancomycin resistant bacteria were screened using the culture-based screening method. In this method, sterile nutrient medium was supplemented with different concentrations of the vancomycin antibiotics. Inoculation of bacterial isolates on the vancomycin supplemented agar followed before plates were incubated. The ensuing bacterial isolates were characterized using biochemical and molecular methods. Ninetine (19) bacteria isolates were screened for vancomycin and of this numbers, six (6) were positive for vancomycin resistance. The agar rose gel electrophoresis showed the presence of vanA and vanB gene which could be responsible for the resistance to the vancomycin observed in most isolates. The presence of vancomycin resistant genes in these isolates is of public health concern due to the transfer of resistance to other isolates. Standard hygienic practice is therefore recommended in the use of well water.

scholarly journals Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

2016 ◽  
Vol 37 (2) ◽  
pp. 829
Author(s):  
Patricia Alves de Oliveira ◽  
Rodrigo Assunção Moura ◽  
Graziela Vendrame Rodrigues ◽  
Karoline Franciani Cardoso Lopes ◽  
Melissa Marchi Zaniolo ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Brain Heart Infusion ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Bacterial Isolates ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Healthy Sheep ◽  
Extended Spectrum Beta Lactamases

Bacterial resistance is a primary public health concern worldwide. Within this context, pets and breeding animals act as reservoirs for multidrug-resistant bacteria (MR), such as those producing extended spectrum beta-lactamases (ESBL) and those presenting plasmid-mediated quinolone resistance (PMQR). The aim of this study was to detect the presence of ESBL and PMQR in members of the Enterobacteriaceae family, isolated from healthy sheep and dogs from non-intense farming rural properties in the Umuarama region of Paraná, Brazil. A total of 81 oral and rectal swabs from dogs and sheep from 11 small rural properties were analyzed. These swabs were inoculated in tubes containing brain heart infusion broth (BHI), and the resulting cultures were inoculated on MacConkey agar (MAC) supplemented with 10 ?g/mL cefotaxime for the selection of ESBL producers. The cells were also plated on MAC supplemented with 50 ?g/mL nalidixic acid for selecting quinolone-resistant enterobacteria. The bacterial isolates were subjected to biochemical identification tests, antibiograms, double-disk synergic tests, and polymerase chain reaction analysis for resistance-inducing genes (blaESBL, qnr, and genes encoding efflux pump and acetylases). Four (5.00%) bacterial isolates (3 Escherichia coli and 1 Morganella morganii) resistant to cephalosporins and/or quinolones were identified; of these, three (75%) isolates were from sheep and one (25%) from a dog. These findings indicate the presence of MR bacteria in the normal microbiota of the animals studied. Animals colonized with such bacteria can contribute to the dissemination of antimicrobial resistance to other animals, environment, and/or human beings and can harbor endogenous infections in unfavorable conditions, which have poor prognosis due to the limited therapeutic options.

scholarly journals A public health concern: emergence of carbapenem-resistant Klebsiella pneumoniae in a public transportation environment

2020 ◽  
Vol 75 (10) ◽  
pp. 2769-2772
Author(s):  
Tingting Cao ◽  
Yuanyuan Liu ◽  
Yiming Li ◽  
Yang Wang ◽  
Zhangqi Shen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Public Transportation ◽  
Genetic Relationships ◽  
Brain Heart Infusion ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Carbapenem Resistant ◽  
Broth Microdilution Method ◽  
Carbapenem Resistant Enterobacteriaceae ◽  
Beijing Subway

Abstract Objectives This study was designed to understand the prevalence of antibiotic-resistant bacteria in the Beijing subway environment and the potential transmission of carbapenem-resistant Enterobacteriaceae in a public transportation environment. Methods Carbapenem-resistant isolates were selected on brain heart infusion agar supplemented with meropenem (0.5 mg/L) and antimicrobial susceptibility testing was conducted using the broth microdilution method. WGS analyses were conducted for 11 Klebsiella pneumoniae isolates to identify resistance genes. The genetic relationships among the isolates were evaluated by MLST and PFGE. Results We identified 11 carbapenem-resistant K. pneumoniae isolates from the Beijing subway environment. WGS revealed three STs among the 11 isolates, with 9 isolates classified as ST726 and containing a blaNDM-5-carrying IncX3 plasmid. The genetic environment of blaNDM-5 was very similar to that observed in other blaNDM-5-containing clinical isolates. Conclusions The presence of carbapenem-resistant Enterobacteriaceae in a public transportation environment is concerning and indicates that regular antimicrobial resistance surveillance is urgent and necessary.

scholarly journals Resistance Rates of the most Isolated Bacteria from different Clinical Samples, Kerbala, Iraq

2020 ◽  
Vol 10 (03) ◽  
pp. 426-430
Author(s):  
Suhad Hadi Mohammed ◽  
Maysaa Saleh Mahdi ◽  
Mohanad Mohsin Ahmed ◽  
Ali Najm Al-Deen ◽  
Nargis Fadhil ◽  
...  
Keyword(s):  
Generation Cephalosporin ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Methicillin Resistant ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Admission Status ◽  
Vancomycin Resistant ◽  
Resistance Rates

Determining the bacterial causative agents of infections by identifying their antimicrobial patterns will enable health institutions to limit the unnecessary use of antibiotics, and take active ways in preventing the spread of drug-resistant bacteria. This study aimed to identify the most common bacterial isolates responsible for infection and their antibiotic resistance rates. The results showed that Escherichia coli, Staphylococcus aureus (S aureus), and Pseudomonas aeruginosa (P. aeruginosa) represent the most common bacteria isolated with a percentage of 23.9, 18.8, and 16.2%, respectively. High resistance rates were found for the most common bacterial isolates. Other important findings are the presence of extended-spectrum B-lactamase (ESBL) producing bacteria and the appearance of hetero-resistance phenomenon. Moreover, the bacterial infection is mainly occurring in men. No significant correlation was observed in the type of isolated bacteria with patient admission status. E. coli strains were found to be highly resistant to amoxicillin-clavulanic acid, ceftriaxone (88.9%), ceftazidime (85.2%), trimethoprim-sulfamethoxazole (74.1%), and ciprofloxacin (59.3%). Whereas, the highest sensitivity rates were seen with meropenem antibiotic (92.6%). Concerning S. aureus isolates, 100, and approximately 91% of resistant rates were seen to penicillin and cefoxitin, respectively [methicillin-resistant S. aureus (MRSA)]. Approximately 50% of MRSA were vancomycin-resistant S. aureus (VRSA). Resistant rates of P. aeruginosa isolates to gentamycin and ciprofloxacin were 47.1%, amikacin 41.2%, and levofloxacin 35.3%. In conclusion, the current study might reveal that the isolated bacteria could be of critical priority carbapenem-resistant P. aeruginosa, and carbapenem-resistant and 3rd generation cephalosporin-resistant E. coli. In addition, the isolation of high priority bacteria includes vancomycin-resistant methicillin-resistant S. aureus.

Opposite effect of vancomycin and D-Cycloserine combination in both vancomycin resistant Staphylococcus aureus and enterococci

2020 ◽  
Vol 367 (8) ◽  
Author(s):  
Abdelhakim Boudrioua ◽  
Yanyan Li ◽  
Axel Hartke ◽  
Caroline Giraud
Keyword(s):  
Staphylococcus Aureus ◽  
Opposite Effect ◽  
Inhibitory Concentration ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Glycopeptide Antibiotic ◽  
Antibiotic Resistant ◽  
Peptidoglycan Biosynthesis ◽  
Vancomycin Resistant ◽  
Type Strains

ABSTRACT The increasing spread of antibiotic resistant bacteria is a major human health concern. The challenging development of new effective antibiotics has led to focus on seeking synergistic antibiotic combinations. Vancomycin (VAN) is a glycopeptide antibiotic used to treat Staphylococcus aureus and enterococci infections. It is targeting D-Alanyl-D-Alanine dimers during peptidoglycan biosynthesis. D-cycloserine (DCS) is a D-Alanine analogue that targets peptidoglycan biosynthesis by inhibiting D-Alanine:D-Alanine ligase (Ddl). The VAN-DCS combination was found to be synergistic in VAN resistant S. aureus strains lacking van genes cluster. We hypothesize that this combination leads to opposite effects in S. aureus and enterococci strains harboring van genes cluster where VAN resistance is conferred by the synthesis of modified peptidoglycan precursors ending in D-Alanyl-D-Lactate. The calculated Fractional Inhibitory Concentration of VAN-DCS combination in a van- vancomycin-intermediate, VanA type, and VanB type strains were 0.5, 5 and 3, respectively. As a result, VAN-DCS combination leads to synergism in van-lacking strains, and to antagonism in strains harboring van genes cluster. The VAN-DCS antagonism is due to a mechanism that we named van-mediated Ddl inhibition bypass. Our results show that antibiotic combinations can lead to opposite effects depending on the genetic backgrounds.

Novel method of calculating adjusted antibiotic use by microbiological burden

2021 ◽  
pp. 1-6
Author(s):  
Hana R. Winders ◽  
Majdi N. Al-Hasan ◽  
Bruce M. Jones ◽  
Darrell T. Childress ◽  
Kayla R. Stover ◽  
...  
Keyword(s):  
Antibiotic Use ◽  
Cross Sectional Study ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Sectional Study ◽  
Cross Sectional ◽  
Vancomycin Resistant Enterococci ◽  
Days Of Therapy ◽  
Novel Method ◽  
Vancomycin Resistant

Abstract Objective: To determine the usefulness of adjusting antibiotic use (AU) by prevalence of bacterial isolates as an alternative method for risk adjustment beyond hospital characteristics. Design: Retrospective, observational, cross-sectional study. Setting: Hospitals in the southeastern United States. Methods: AU in days of therapy per 1,000 patient days and microbiologic data from 2015 and 2016 were collected from 26 hospitals. The prevalences of Pseudomonas aeruginosa, extended-spectrum β-lactamase (ESBL)–producing bacteria, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant enterococci (VRE) were calculated and compared to the average prevalence of all hospitals in the network. This proportion was used to calculate the adjusted AU (a-AU) for various categories of antimicrobials. For example, a-AU of antipseudomonal β-lactams (APBL) was the AU of APBL divided by (prevalence of P. aeruginosa at that hospital divided by the average prevalence of P. aeruginosa). Hospitals were categorized by bed size and ranked by AU and a-AU, and the rankings were compared. Results: Most hospitals in 2015 and 2016, respectively, moved ≥2 positions in the ranking using a-AU of APBL (15 of 24, 63%; 22 of 26, 85%), carbapenems (14 of 23, 61%; 22 of 25; 88%), anti-MRSA agents (13 of 23, 57%; 18 of 26, 69%), and anti-VRE agents (18 of 24, 75%; 15 of 26, 58%). Use of a-AU resulted in a shift in quartile of hospital ranking for 50% of APBL agents, 57% of carbapenems, 35% of anti-MRSA agents, and 75% of anti-VRE agents in 2015 and 50% of APBL agents, 28% of carbapenems, 50% of anti-MRSA agents, and 58% of anti-VRE agents in 2016. Conclusions: The a-AU considerably changes how hospitals compare among each other within a network. Adjusting AU by microbiological burden allows for a more balanced comparison among hospitals with variable baseline rates of resistant bacteria.

scholarly journals High Carriage of Plasmid-Mediated Quinolone Resistance (PMQR) Genes by Cefotaxime-Resistant Escherichia Coli Recovered from Surface-Leaking Sanitary Sewers

2021 ◽  
Author(s):  
Abimbola Olumide Adekanmbi ◽  
Olabisi C. Akinlabi ◽  
Adedolapo V. Olaposi
Keyword(s):  
Escherichia Coli ◽  
Brain Heart Infusion ◽  
Quinolone Resistance ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Specific Pcr ◽  
Sanitary Sewers ◽  
High Level

Abstract There is a rapid rise in the incident of quinolone resistant bacteria in Nigeria. Most studies in Nigeria have focused on isolates from the clinical settings, with few focusing on isolates of environmental origin. This study aimed to investigate the antibiogram and carriage of plasmid-mediated quinolone resistance (PMQR) genes by quinolone-resistant isolates obtained from a pool of cefotaxime-resistant Escherichia coli (E. coli) recovered from sewage leaking out of some broken sanitary sewers in a University community in Nigeria. Isolation of E. coli from the sewage samples was done on CHROMagar E. coli after enrichment of the samples was done in Brain Heart Infusion amended with 6µg/mL of cefotaxime. Identification of presumptive E. coli was done using molecular methods (detection of uidA gene), while susceptibility to antibiotics was carried out using disc-diffusion method. Detection of PMQR genes (qnrA, qnrB, qnrS, aac(6')-lb-cr, qepA and oqxAB) was done using primer-specific PCR. A total of 32 non-repetitive cefotaxime-resistant E. coli were obtained from the sewage, with 21 being quinolone-resistant. The quinolone-resistant isolates showed varying level of resistance to the tested antibiotics, with imipenem being the only exception with 0% resistance. The PMQR genes: aac(6')-lb-cr, qnrA, qnrB, qnrS and qepA and oqxAB were detected in 90.5%, 61.9%, 47.6%, 38.1%, 4.8% and 0% respectively of the isolates. The findings of this study showed a high level of resistance to antibiotics and carriage of PMQR genes by quinolone-resistant E. coli obtained from the leaking sanitary sewers, suggesting a potential environmental and public health concern.

scholarly journals First report of a linezolid-resistant MRSA (methicillin resistant Staphylococcus aureus) isolated from a dog with a severe bilateral otitis in Portugal

2011 ◽  
Vol 22 (2) ◽  
pp. 81
Author(s):  
R. Seixas ◽  
V. Monteiro ◽  
C. Carneiro ◽  
C. L. Vilela ◽  
M. Oliveira
Keyword(s):  
Staphylococcus Aureus ◽  
Veterinary Medicine ◽  
Antibiotic Therapy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
New Drugs ◽  
Health Concern ◽  
Resistant Bacteria ◽  
First Report ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

<p class="Pa5"><span lang="EN-US">The widespread use of antimicrobials has lead to the emergence of resistant bacteria to one or more antibiotic, including new drugs like linezolid. This antimicrobial is the first of the oxazolidinone group and soon after its approval in 2000, linezolid-resistant MRSA and linezolid vancomycin-resistant enterococci have emerged. Several outbreaks of linezolid-resistant MRSA have been reported worldwide but, to our knowledge, this is the first report of a linezolid-resistant MRSA isolated from a dog in Portugal. The animal arrived at the Teaching Hospital of the Faculty of Veterinary Medicine, Technical University of Lisboa with a severe bilateral otitis that was refractory to antibiotic therapy. Bacteriology showed that the infection was caused by a multiresistant <em>Staphylococcus aureus </em>strain that also phenotipically expressed other virulence factors. Besides the challenge to practitioners, the isolation of this strain is of pub­lic health concern due to its antimicrobial resistant profile. </span></p>

scholarly journals Prevalence and Antibiogram of Gram-negative Bacteria Isolated from Well Water in Ula-Ubie Community, Ahoada West, Nigeria

2020 ◽  
pp. 1-10
Author(s):  
R. R. Nrior ◽  
M. Okpokiri ◽  
N. P. Akani
Keyword(s):  
Public Health ◽  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Physicochemical Parameters ◽  
Heterotrophic Bacteria ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Well Water ◽  
Bacterial Isolates ◽  
Microbiological Methods

Antibiotic resistance has become a great global problem. Thus, it has emerged as a public health challenge. The antibiotic susceptibility pattern of bacteria in well water was characterized with a view of determining the level of resistance in the environment. Fifty well water samples were collected from ten different points in Ula-Ubie community, Ahoda, Rivers State for a period of five months. Standard microbiological methods were used to analyse the population and types of bacteria in the water while methods recommended by the American Public Health Association (APHA) was used to determine the physicochemical parameters of the samples. The antibiotic susceptibility profile of the bacterial isolates was carried out using the disc diffusion methods. The total heterotrophic bacteria of the water samples ranged from 0.93±0.46 to 2.02±1.06 log10 CFU/ml. The coliform counts ranged from 0.45±0.42 - 2.55±2.33 log10 CFU/ml, respectively. Despite the variations in the counts of the different bacterial population, there was no significant differences (P > 0.05) in the different well water samples. The physicochemical parameters except the pH were all within the permissible limits. Klebsiella spp, Pseudomonas spp, Serratia spp and Enterobacter spp were identified in the well water. The pH of the water stations ranged from 4.66 to 5.80. The temperature ranged from 24.0 to 24.7. The electrical conductivity, salinity, dissolved oxygen, total hardness, alkalinity, total suspended solids, biochemical oxygen demand, nitrate, chloride, calcium and magnesium ranged from 22.9 – 219, 0.03-0.13, 4.50-4.90, 5.00-22.0, ˂0.01-3.00, ˂0.01, 49.6-84.5, 1.00-17.4, 3.00-24.5, 4.25-12.9 and 0,722-1.55 respectively. The antibiotic susceptibility profile showed that all the isolates were resistant to ceftazidime and augmentin, whereas Enterobacter spp were the most resistant bacteria amongst other bacterial genera to the antibiotics. Meanwhile there is an existence of multi-drug resistance. Thus, the wells could be considered not potable due to the presence of these bacterial isolates and the level of antibiotic resistant. Proper sanitation and cleanliness of well should be encouraged.

scholarly journals Prevalence of Multidrug-Resistant Bacteria Isolates in Waste Water from Different Hospital Environment in Umuahia, Nigeria

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Uzoije U.N ◽  
Iroha I.R ◽  
Moses I.B ◽  
Ukwa B.N ◽  
Onuorah A.L ◽  
...  
Keyword(s):  
Public Health ◽  
Waste Water ◽  
Water Samples ◽  
Multidrug Resistant ◽  
Hospital Environment ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Hospital Waste

The discharge of untreated hospital waste water into the environment is a major public health concern as this could result in the environmental spread of multidrug-resistant (MDR) bacteria. The dissemination of such MDR bacteria in waste water remains unexamined in most geographical area. This study assessed the prevalence of multidrug-resistant bacterial isolates in waste water from hospital environment in Umuahia, Nigeria. Exactly 200 waste water samples were collected from various diagnostic laboratory units of Michael Okpara University of Agriculture, Umudike (MOUAU) Health Centers and Federal Medical Center (FMC), Umuahia in 250 ml screw-capped, heat-sterilized bottles. Water samples were analyzed using standard microbiological techniques. Bacterial isolates from water samples were identified with API-20E test kit. Antibiotic susceptibility test was done using Kirby–Bauer disc diffusion method. Multiple antibiotic resistance index (MARI) of isolated bacteria was determined using standard formulae. A total of 147(73.5 %) bacterial species such as S. aureus, Shigella spp, E. coli, Enterobacter spp, Proteus mirabilis, and Arizona spp were identified from the waste water samples. Bacterial isolates exhibited resistance to tetracycline, trimethoprim-sulfamethoxazole, clindamycin, nalixidic acid, vancomycin, and chloramphenicol but very susceptible to imipenem. All isolates were multidrug-resistant with MARI values ranging from 0.5 - 0.8. This study revealed the presence of MDR bacteria in hospital waste water samples in Umuahia, Abia state, Nigeria. The threat and risk of exposure to such MDR bacteria is of public health significance and raises concern over poor management and disposal of hospital waste water or effluents.

DIVERSITY OF CULTURABLE BACTERIAL MICROBIOTA OF THE Eisenia foetida DIGESTIVE TRACT

2018 ◽  
Vol 41 (3) ◽  
pp. 255-264 ◽  
Author(s):  
J. Abraham Pérez-Pérez ◽  
David Espinosa-Victoria ◽  
Hilda V. Silva-Rojas ◽  
Lucía López-Reyes
Keyword(s):  
Bacterial Diversity ◽  
Digestive Tract ◽  
Bacterial Species ◽  
Brain Heart Infusion ◽  
Rrna Gene ◽  
Eisenia Foetida ◽  
Bacterial Isolates ◽  
Bacterial Microbiota ◽  
Decomposition Of Organic Matter ◽  
Earthworm Gut

Bacteria are an unavoidable component of the natural earthworm diet; thus, bacterial diversity in the earthworm gut is directly linked to decomposition of organic matter and development of the surrounding plants. The aim of this research was to isolate and to identify biochemically and molecularly the culturable bacterial microbiota of the digestive tract of Eisenia foetida. Earthworms were sourced from Instituto de Reconversión Productiva y Bioenergética (IRBIO) and Colegio de Postgraduados (COLPOS), México. Bacterial isolation was carried out on plates of Brain Heart Infusion (BHI) culture medium. Fifty six and 44 bacterial isolates were obtained from IRBIO and COLPOS, respectively. The population was composed of 44 Gram-negative and 56 Gram-positive isolates. Over 50 % of the bacterial isolates were rod-shaped cells. The 16S rRNA gene was sequenced and nine genera were identified in worms from IRBIO (Bacillus, Paenibacillus, Solibacillus, Staphylococcus, Arthrobacter, Pantoea, Stenotrophomonas, Acinetobacter and Aeromonas) and six in worms from COLPOS (Bacillus, Paenibacillus, Stenotrophomonas, Staphylococcus, Acinetobacter and Aeromonas). Bacillus was the predominant genus, with eight and six species in the oligochaetes from IRBIO and COLPOS, respectively. The most represented bacteria in the worms from both sites were Bacillus sp. and B. subtilis. The predominance of Bacillus was probably due to spore formation, a reproductive strategy that ensures survival and dispersion in the soil and oligochaetes digestive tract. The gut of E. foetida not only harbored bacterial species of agronomic importance but also species potentially pathogenic for humans (Staphylococcus warneri, Pantoea agglomerans and Stentrophomonas sp.). The larger bacterial diversity in worms from IRBIO could be due to their feeding on cattle manure, which is a rich source of bacteria.

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