Anti-Quorum Sensing Effects of Some Medicinal and Aromatic Plant Extracts on Xanthomonas axonopodis pv. Phaseoli

Xanthomonas axonopodis pv. phaseoli (Xap) is known as one of the most important seed-borne destructive pathogens on beans worldwide. Nowadays, Xap is considered to acquire resistance to antibiotics and synthetic bactericides which concerns the scientific world for its future management. This has made the use of plant extracts, the best alternative in the control of plant disease pathogens by inhibiting the quorum sensing (QS) mediated virulence factors. This research was designed to investigate the antibacterial activities and the anti-QS effects of the 14 different aromatic and medicinal plants against QS-mediated virulence factors of Xap. The results revealed that Syzgium aromaticum showed the largest inhibition zone diameter and strongest antimicrobial (antibacterial) effect among the 14 plant extracts followed by Thymus vulgaris and Coriandrum sativum. Similarly, the lowest swarming, swimming, and twitching motility values were measured from the Syzgium aromaticum application followed by Coriandrum sativum, Thymus vulgaris, Brassica nigra, Lepidium sativum, and Ruta chalepenis. These results indicated that S. aromaticum, C. sativum, T. vulgaris, B. nigra, L. sativum, and R. chalepenis will be a potential candidates as anti-quorum sensing agents in preventing common bacterial disease of beans caused by Xap. Compounds derived from aromatic and medicinal plants have demonstrated successful control of diseases in crops and the use of these substances provides a valuable tool to the growers around the world for diseases management in organic production.

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Interaction of Folk Medicinal Plants with Levofloxacin against Escherichia Coli

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.3.24 ◽

2020 ◽

Vol 14 (3) ◽

pp. 1855-1861

Author(s):

Isra Tayseer ◽

Hanan Azzam ◽

Nehaya Al-Karablieh ◽

Amal Mayyas ◽

Talal Aburjai

Keyword(s):

Escherichia Coli ◽

Medicinal Plants ◽

Plant Extracts ◽

Curcuma Longa ◽

Antimicrobial Activities ◽

Thymus Vulgaris ◽

Additive Interaction ◽

Traditional Use ◽

E Coli

The present study was conducted to assess the in vitro activities of folk medicinal plants in combination with levofloxacin against TG1 and mutant KAM3-1(∆acrB-∆tolC) Escherichia coli strains. Plants were chosen based on their traditional use in combination with antibiotics among laymen. Standard protocols were followed to examine the antimicrobial activity of plant extracts and levofloxacin against E. coli in term of their minimum inhibitory concentrations (MICs) and to evaluate the plant extracts-levofloxacin interaction using checkerboard method. Among the twelve plants investigated, Thymus vulgaris, Zingiber officinale, Teucrium polium, Matricaria chamomilla and Curcuma longa had the best antimicrobial activities against E. coli strains with MIC values at 250 μg/ml. It is noteworthy to mention that other folk plants extracts reveled no effects against E coli strains. Furthermore, additive interactions were observed between levofloxacin and T. polium or T. vulgaris against E. coli wild-type TG1 strain. There was no antagonism being observed in this study. The detection of additive interaction between the extracts and levofloxacin demonstrates the prospective of these folk medicinal plants as a source of compounds to modulate antibiotic resistance.

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An ethnobotanical survey of spice, aromatic and medicinal plants used in La Molana, Atrato-Choco, Colombia: Basis for biodiversity conservation

Acta Agronómica ◽

10.15446/acag.v67n1.59316 ◽

2018 ◽

Vol 67 (1) ◽

pp. 9-16

Author(s):

Miller Marmolejo Liloy ◽

Jesús David Ponce Mendoza ◽

Leidy Indira Hinestroza Córdoba ◽

Harold Moreno Holguín

Keyword(s):

Medicinal Plants ◽

Biodiversity Conservation ◽

Ocimum Basilicum ◽

Coriandrum Sativum ◽

Origanum Vulgare ◽

Ethnobotanical Survey ◽

Aromatic And Medicinal Plants ◽

Eryngium Foetidum

Se realizó la caracterización y análisis de los sistemas productivos de plantas aromáticas, medicinales y condimentarias (PAMC) de La Molana, Atrato-Chocó, Colombia a través de la identificación de sus características biofísicas, socioeconómicas y financieras, con el fin de proponer alternativas agroecológicas que solucionen los problemas y necesidades identificados en dichos sistemas; aplicando las metodologías e indicadores de evaluación de sistemas agrícolas hacia el desarrollo sostenible, Diseño, implementación y evaluación de arreglos agroforestales, Sistemas Agroforestales acompañado del análisis DOFA, y planificación agroforestal de unidades productivas (PAF), dicha información fue recopilada a través de la aplicación de un taller focal y cuestionarios semiestructurados a 40 productores. Se caracterizaron ocho unidades productivas (UP), cuatro parcelas y cuatro azoteas, a través de un muestreo aleatorio simple sin reposición. Los resultados señalan que estos productores destinan 15.6 ha a la producción de PAMC, de las cuales 11 ha son de cultivos en parcelas dentro de bosques y 4.6 ha son destinadas a cultivos en azoteas alrededor de las viviendas. Las unidades producen en promedio, 110 unidades (manojos) semanales que son ofrecidos a vendedores informales en la plaza de mercado de Quibdó-Chocó, Colombia a precios que van desde COP $1000 hasta COP $3000 de acuerdo al tamaño y la especie. De igual modo se determinó que los ingresos económicos de las UP de PAMC dependen principalmente de las siguientes especies sembradas en las parcelas: Cilantro (Coriandrum sativum L.), cimarrón (Eryngium foetidum L.), Albahaca (Ocimum basilicum L.) y Orégano (Origanum vulgare L.), las cuales son las especies de mayor importancia económica en la zona de estudio.

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Study of the inhibitory effect of the ethanolic extract of a number of local medicinal plants on the growth of proteus spp. in vitro

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v37i1.329 ◽

2013 ◽

Vol 37 (1) ◽

pp. 40-46

Author(s):

Jinan Abdul-Amir Sabeeh

Keyword(s):

Antibacterial Activity ◽

Medicinal Plants ◽

Inhibitory Activity ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

The Other ◽

Coriandrum Sativum ◽

Thymus Vulgaris

The present study was designed to evaluate the inhibitory effect of six local medicinal plants ethanolic extract ( Quercusrobur, Vitisvinifera, Cinamomumzeylanicum , Ginger officinale ,Thymus vulgaris and Coriandrumsativum ) on the growth of Proteus spp. in vitro. For this purpose graduate concentrates for each extract (50, 100, 200 and 400) mg/ml were prepared and tested. The result showed that all the studied medicinal plants have antibacterial activity against Proteus spp. with exception for Coriandrumsativum which didn’t showed any inhibitory activity for the growing of the studied bacteria in Vitro. And the more active plant in inhibition the growth of the studied bacteria was Quercusrobur followed by Vitisvinifera and Cinamomum zeylanicum , Ginger officinale ,Thymus vulgaris. Also, there was no significant differences between the effect of the different concentrations of Thymus vulgaris and Coriandrum sativum, while there were a significant differences between effect of Cinamomumzeylanicum concentrations and the concentrations of (400 and 200 mg\ml) Ginger officinale and compared with 100 and 50 mg/ml of Quercusrobur, while, the ethanolic extract of the Vitisvinifera showed a significant differences between the effect of the concentration 400 mg\ml with the other studied concentration.

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Attenuation of quorum-sensing-dependent virulence factors and biofilm formation by medicinal plants against antibiotic resistant Pseudomonas aeruginosa

Journal of Traditional and Complementary Medicine ◽

10.1016/j.jtcme.2017.05.008 ◽

2018 ◽

Vol 8 (1) ◽

pp. 170-177 ◽

Cited By ~ 19

Author(s):

P. Sankar Ganesh ◽

V. Ravishankar Rai

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Medicinal Plants ◽

Virulence Factors ◽

Biofilm Formation ◽

Antibiotic Resistant

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QUORUM SENSING SCREENING OF SOME MEDICINAL PLANTS FROM MONGOLIA

Mongolian Journal of Agricultural Sciences ◽

10.5564/mjas.v13i2.518 ◽

2015 ◽

Vol 13 (2) ◽

pp. 63-65 ◽

Cited By ~ 1

Author(s):

B Zolboo ◽

L Tsenguunmaa ◽

O Undram ◽

J Batkhuu

Keyword(s):

Quorum Sensing ◽

Medicinal Plants ◽

Plant Extracts ◽

Inhibitory Activity ◽

Aerial Part ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Bacterial Communication ◽

Standard Disc

The purpose of this study was to search for a novel quorum sensinginhibitor and analyseits inhibitory activity of medicinal plants of Mongolia. This study investigated the effects of some plant extracts on the bacterial communication system, expressed as quorum sensing activity. Quorum sensinghas directly propotional effect on the amount of certain compound such as pigments, produced by the bacteria. Alcohol extracts of 103 extracts of 66 medicinal plants were tested for anti-quorum sensingactivity by the Chromobacterium violaceum assay using the standard disc-diffusion method. The screening revealed the anti-quorum sensing activity of 18 extracts of 13 plants; particularly aerial part of Hedysarum alpinum L., Spongiocarpella gruboviiUlzii and Goniolimon speciosum (L.) Boiss.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 63-65

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Natural products from the traditional Moroccan pharmacopoeia: A potential lead for the prevention and treatment of COVID-19

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl1.3619 ◽

2020 ◽

Vol 11 (SPL1) ◽

pp. 1278-1285

Author(s):

Mohamed Yafout ◽

Amine Ousaid ◽

Ibrahim Sbai El Otmani ◽

Youssef Khayati ◽

Amal Ait Haj Said

Keyword(s):

Medicinal Plants ◽

Plant Extracts ◽

Molecular Mechanisms ◽

Scientific Literature ◽

Treatment Protocol ◽

World Health ◽

The World ◽

Health Organization ◽

Promising Source

The new SARS-CoV-2 belonging to the coronaviruses family has caused a pandemic affecting millions of people around the world. This pandemic has been declared by the World Health Organization as an international public health emergency. Although several clinical trials involving a large number of drugs are currently underway, no treatment protocol for COVID-19 has been officially approved so far. Here we demonstrate through a search in the scientific literature that the traditional Moroccan pharmacopoeia, which includes more than 500 medicinal plants, is a fascinating and promising source for the research of natural molecules active against SARS-CoV-2. Multiple in-silico and in-vitro studies showed that some of the medicinal plants used by Moroccans for centuries possess inhibitory activity against SARS-CoV or SARS-CoV-2. These inhibitory activities are achieved through the different molecular mechanisms of virus penetration and replication, or indirectly through stimulation of immunity. Thus, the potential of plants, plant extracts and molecules derived from plants that are traditionally used in Morocco and have activity against SARS-CoV-2, could be explored in the search for a preventive or curative treatment against COVID-19. Furthermore, safe plants or plant extracts that are proven to stimulate immunity could be officially recommended by governments as nutritional supplements.

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Quorum Sensing – A Stratagem for Conquering Multi-Drug Resistant Pathogens

Current Pharmaceutical Design ◽

10.2174/1381612826666201210105638 ◽

2020 ◽

Vol 26 ◽

Author(s):

Madison Tonkin ◽

Shama Khan ◽

Mohmmad Younus Wani ◽

Aijaz Ahmad

Keyword(s):

Drug Resistance ◽

Quorum Sensing ◽

Virulence Factors ◽

Biofilm Formation ◽

Cell Communication ◽

Natural Compounds ◽

Quorum Sensing Inhibitors ◽

Communication Technique ◽

Multicellular Organisms ◽

Chemical Strategies

: Quorum sensing is defined as cell to cell communication between microorganisms, which enables microorganisms to behave as multicellular organisms. Quorum sensing enables many collaborative benefits such as synchronisation of virulence factors and biofilm formation. Both quorum sensing as well as biofilm formation encourage the development of drug resistance in microorganisms. Biofilm formation and quorum sensing are causally linked to each other and play role in the pathogenesis of microorganisms. With the increasing drug resistance against the available antibiotics and antifungal medications, scientists are combining different options to develop new strategies. Such strategies rely on the inhibition of the communication and virulence factors rather than on killing or inhibiting the growth of the microorganisms. This review encompasses the communication technique used by microorganisms, how microorganism resistance is linked to quorum sensing and various chemical strategies to combat quorum sensing and thereby drug resistance. Several compounds have been identified as quorum sensing inhibitors and are known to be effective in reducing resistance as they do not kill the pathogens but rather disrupt their communication. Natural compounds have been identified as anti-quorum sensing agents. However, natural compounds present several related disadvantages. Therefore, the need for the development of synthetic or semi-synthetic compounds has arisen. This review argues that anti-quorum sensing compounds are effective in disrupting quorum sensing and could therefore be effective in reducing microorganism drug resistance.

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Pseudomonas aeruginosa PA80 is a cystic fibrosis isolate deficient in RhlRI quorum sensing

Scientific Reports ◽

10.1038/s41598-021-85100-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Syed A. K. Shifat Ahmed ◽

Michelle Rudden ◽

Sabrina M. Elias ◽

Thomas J. Smyth ◽

Roger Marchant ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Virulence Factors ◽

Genomic Islands ◽

Clinical Strain ◽

Whole Genome ◽

Synonymous Mutations ◽

Wide Range

AbstractPseudomonas aeruginosa uses quorum sensing (QS) to modulate the expression of several virulence factors that enable it to establish severe infections. The QS system in P. aeruginosa is complex, intricate and is dominated by two main N-acyl-homoserine lactone circuits, LasRI and RhlRI. These two QS systems work in a hierarchical fashion with LasRI at the top, directly regulating RhlRI. Together these QS circuits regulate several virulence associated genes, metabolites, and enzymes in P. aeruginosa. Paradoxically, LasR mutants are frequently isolated from chronic P. aeruginosa infections, typically among cystic fibrosis (CF) patients. This suggests P. aeruginosa can undergo significant evolutionary pathoadaptation to persist in long term chronic infections. In contrast, mutations in the RhlRI system are less common. Here, we have isolated a clinical strain of P. aeruginosa from a CF patient that has deleted the transcriptional regulator RhlR entirely. Whole genome sequencing shows the rhlR locus is deleted in PA80 alongside a few non-synonymous mutations in virulence factors including protease lasA and rhamnolipid rhlA, rhlB, rhlC. Importantly we did not observe any mutations in the LasRI QS system. PA80 does not appear to have an accumulation of mutations typically associated with several hallmark pathoadaptive genes (i.e., mexT, mucA, algR, rpoN, exsS, ampR). Whole genome comparisons show that P. aeruginosa strain PA80 is closely related to the hypervirulent Liverpool epidemic strain (LES) LESB58. PA80 also contains several genomic islands (GI’s) encoding virulence and/or resistance determinants homologous to LESB58. To further understand the effect of these mutations in PA80 QS regulatory and virulence associated genes, we compared transcriptional expression of genes and phenotypic effects with isogenic mutants in the genetic reference strain PAO1. In PAO1, we show that deletion of rhlR has a much more significant impact on the expression of a wide range of virulence associated factors rather than deletion of lasR. In PA80, no QS regulatory genes were expressed, which we attribute to the inactivation of the RhlRI QS system by deletion of rhlR and mutation of rhlI. This study demonstrates that inactivation of the LasRI system does not impact RhlRI regulated virulence factors. PA80 has bypassed the common pathoadaptive mutations observed in LasR by targeting the RhlRI system. This suggests that RhlRI is a significant target for the long-term persistence of P. aeruginosa in chronic CF patients. This raises important questions in targeting QS systems for therapeutic interventions.

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