scholarly journals Compound effect of nitrates and biotic factors on the in vitro activity of epithelial cilia of outer demibranchs of Sinanodonta woodiana (Mollusca, Bivalvia, Unionidae)

2020 ◽  
pp. 129-135
Author(s):  
A. Stadnychenko ◽  
◽  
O. Uvayeva ◽  
A. Vyskushenko ◽  
◽  
...  
Keyword(s):  
Beat Frequency ◽  
Water Bodies ◽  
Control Group ◽  
Ciliary Epithelium ◽  
Biotic Factors ◽  
Biotic Factor ◽  
Functional Parameters ◽  
Trematode Infection ◽  
Density And Biomass ◽  
Experimental Pond

Effects of MPC, 2 MPC, and 3MPC water solutions of potassium nitrate and biotic factors (trematode infection and “gill pregnancy”) were studied on the duration of locomotion and beat frequency of epithelial cilia of outer demibranchs of Sinanodonta woodiаna (Lea, 1834). The species has recently invaded the system of water bodies of Ukraine. It has occupied numerous water bodies in the Kiliya distributary of Danube Delta, establishing populations of density and biomass. Statistically significant differences by the studied parameters of ciliary epithelium (duration of locomotion and beat frequency of cilia) were not observed in the control group of non-infected males and latent females of S. woodiana. In females with “gill pregnancy”, the duration of ciliary locomotion decreased by 1.5 times, and beat frequency by 1.4 times (P>99.9 %). It should be noted that the outer surface of demibranchs of female mollusks was completely covered by the filled to the brim marsupial pockets. Thus, the functional load of gills is high, leading to the development of compensatory processes in experimental animals. The processes induce increased effectiveness of protective factors that counteract them. Another biotic factor negatively affecting the experimental pond mussels was the high rates of trematode infection in the hosts’ reproductive glands which contained sporocysts and cercariae of Rhipidocotyle campanula Dujardin, 1845. Prevalence of that infection ranged from 50 to 100 %. These rates of infection were associated with sharply declining functions of cilia of the gill epithelium in studied animals. The compound pathogenic influence of parasite and “gill pregnancy” factors even more affected the functional parameters of outer gill ciliary epithelium. The MPC levels of toxicant in the water medium were accompanied with the first symptom of intoxication of the Chinese pond mussel, the stage of stimulation. At that point, the functional parameters of epithelial cilia of “pregnant” females and in moderately and weakly infected mollusks of both sexes increased in response to exposure to the toxic factor. With the growing concentrations of toxicant to 2 MPC and 3 MPC, the experimental pond mussels developed the pathological process of intoxication, which progressed from the stage of depression to sublethal and lethal stages. Accordingly, the locomotion activity of ciliary epithelium increasingly declined and, in the end, stopped. The “gill pregnancy” and high rates of trematode infection exacerbated the consequences of the process.

RELATION OF THE MARKERS OF MYOCARDIAL DYSFUNCTION AND SYSTEMIC INFLAMMATION WITH STRUCTURAL AND FUNCTIONAL PARAMETERS OF THE LUNGS IN MYOCARDIAL INFARCTION PATIENTS

2018 ◽  
Vol 17 (2) ◽  
pp. 24-28
Author(s):  
O. M. Polikutina ◽  
Y. S. Slepynina ◽  
E. D. Bazdyrev ◽  
V. N. Karetnikova ◽  
O. L. Barbarach
Keyword(s):  
Myocardial Infarction ◽  
Systemic Inflammation ◽  
Myocardial Dysfunction ◽  
Control Group ◽  
Chronic Obstructive ◽  
Lung Pathology ◽  
Functional Changes ◽  
Functional Parameters ◽  
Lung Capacity ◽  
Copd Patients

Aim. To evaluate the structural and functional changes in the lungs of ST elevation myocardial infarction (STEMI) patients with absence or presence of chronic obstructive lung disease (COPD), and the relation with myocardial dysfunction and systemic inflammation.Material and methods. Totally, 189 STEMI patients included: group 1 — STEMI with COPD of moderate and mild grade, 2 — STEMI with no lung pathology. Groups were comparable by clinical and anamnestic parameters. Assessment of lung function and blood collection were done at 10­12 day of STEMI. For comparison of the parameters representing structural and functional changes in the lungs and comparison of C­reactive protein (CRP), N­terminal pro­brain natriuretic peptide (NT­proBNP) concentration, a control group was formed with no pulmonary pathology, comparable by age and sex with the STEMI patients.Results. In COPD patients, higher values revealed of the parameters representing the part of residual volumes in pulmonary structure. Higher residual volume (RV) was found also in STEMI and no COPD comparing to controls, however the relation RV/TLC (total lung capacity) was not higher than normal range. In both groups there were lower values of diffusion lung capacity (DLCO) comparing to controls. The lowest DLCO found in COPD patients. Concentration of NT­proBNP (H=41,6; p<0,001) and CRP (H=38,6; p<0,001) in COPD was significantly higher in STEMI with no COPD patients than in controls. The negative correlations found for NT­proBNP and CRP with forced expiratory volume 1 sec, FEV/FVC1, DLCO, and positive — with the values of thoracic volume, RV/TLC.Conclusion. In STEMI patients the increase revealed of residual lung volumes. Mostly the level of residual volumes is high in STEMI and COPD patients. There are associations of NT­proBNP and CRP with structural and functional parameters of the lungs regardless of COPD.

scholarly journals ANALYSIS OF THE DYNAMICS OF FUNCTIONAL OCCLUSION INDICATORS IN THE CONSTRUCTION OF OCCLUSION SURFACES OF ORTHOPEDIC STRUCTURES

2020 ◽  
Vol 73 (12) ◽  
pp. 2651-2656
Author(s):  
Svitlana P. Yarova ◽  
Sergii O. Turchenenko ◽  
Iryna M. Tkachenko ◽  
Yuriy Yu. Yarov
Keyword(s):  
Functional Condition ◽  
Masticatory Muscles ◽  
Main Group ◽  
Suggested Method ◽  
Control Group ◽  
Orthopedic Treatment ◽  
Functional Parameters ◽  
Occlusal Surfaces ◽  
Left And Right ◽  
And Control

The aim: Is to evaluate the effectiveness of the proposed method of constructing occlusal surfaces of orthopedic structures by monitoring the dynamics of functional indicators of occlusion and the functional condition of the masticatory muscles. Materials and methods: The object of the study was made up by 64 patients with included defects of the dentition of the 3rd class according to Kennedy. Patients were divided into two equal groups – the main group (patients were treated by using the suggested method of construction of occlusal surfaces of orthopedic structures) and control (dentition defects were replaced according to the traditional protocol). Results: The results of evaluating the effectiveness of the proposed method showed that the index of symmetry of the anterior bundles of the temporal muscles dropped by 18.5%, the index of symmetry of the masticatory muscles reduced by 22%, the torque of the mandible reduced by 32%, the index of stabilizing occlusal – by 27%, relative efforts of balance disturbance for more than 10 % regarding the left and right reduced by a factor of 4.4, the rate of opening time was 1.5 times higher than before, the rate of time of opening of the jaws – 1.8 times. Conclusions: The use of the suggested method of orthopedic treatment with temporary structures leads to probable changes in the studied functional parameters in comparison with the control group (p <0,05).

Efficiency Control Cellulite Treatment Using High-Frequency Ultrasound Visualization of High Resolution

10.12737/5809 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-2
Author(s):  
Белков ◽  
P. Belkov ◽  
Безуглый ◽  
A. Bezuglyy ◽  
Круглова ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Skin ◽  
High Frequency ◽  
Subcutaneous Tissue ◽  
Control Group ◽  
Objective Data ◽  
High Frequency Ultrasound ◽  
Functional Parameters ◽  
Efficiency Control ◽  
Frequency Ultrasound

To describe ultrasonographic image of human skin with cellulite, the authors used high-frequency ultrasound visualization of the skin with a frequency of 22 MHz using a system DUB (tpm GmbH Germany). The skin on the thigh in 15 patients with cellulite and in 10 patients in the control group was examined. The differences in thickness and acoustic density of dermis and subcutaneous tissue between the group of patients with cellulite and in control were described. Objective data of high frequency ultrasound allow to quantify morphological and functional parameters of the skin in the dynamics and results of cellulite correction.

scholarly journals Treatment Technology of Microbial Landscape Aquatic Plants for Water Pollution

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Ming Hu ◽  
Lei Li
Keyword(s):  
Water Pollution ◽  
Water Quality ◽  
Aquatic Plants ◽  
Rapid Development ◽  
Water Bodies ◽  
Control Group ◽  
Human Beings ◽  
Treatment Technology ◽  
Blank Control Group ◽  
Microbial Inoculant

With the rapid development of industrial and agricultural production, the rapid growth of population, and the acceleration of urbanization, the problem of water pollution is becoming more and more serious. Water shortages and pollution disrupt the balance of ecosystems and seriously limit people’s health and rapid economic development. Nowadays, the method of repairing sewage bodies using microbial landscape aquatic plants is attracting more and more attention, and it is a big challenge to maintain the sustainable development of human beings and nature. This paper uses floating rafts to combine microorganisms and landscape aquatic plants to conduct sewage treatment experiments. According to microorganisms, landscape aquatic plants absorb nutrients in the water body, examine the changes in water quality during the restoration of microorganisms’ landscape aquatic plants, and establish the growth of microorganisms’ landscape aquatic plants. The relationship with changes in water quality aims to provide a theoretical basis for the treatment of slow-flowing water bodies such as lakes, reservoirs, large artificial ponds, and rivers. In this paper, the experiments are divided into four groups (A (experimental sewage + microbial inoculant), B (experimental sewage + plant), C (experimental sewage + microbial inoculant + plant), and D (experimental sewage)). It can be divided into the total nitrogen content, total phosphorus content, and COD value data, and chromaticity detection of each group of the test is continuously monitored weekly to comprehensively detect and observe the repair effect on contaminated water bodies. The experiment proved that the water quality of the three treatment groups was significantly clearer than that of the blank control group, and its clarity: microorganism + plant > microorganism > plant > blank control group. This shows that the combination of microorganisms and landscape aquatic plants can effectively reduce the various pollutants contained in sewage and reduce the color of sewage. Treating sewage using plant technology that combines microorganisms is feasible and promising.

scholarly journals INFLUENCE OF ANTHROPOGENIC AND BIOTIC FACTORS ON THE POPULATION OF BEE COLONIES IN THE RYAZAN REGION

2021 ◽  
pp. 23-30
Author(s):  
Е.О. ЛАРЬКИНА ◽  
Д.В. СВИЩУК ◽  
Е.П. ЛАПЫНИНА
Keyword(s):  
Honey Bee ◽  
Honey Bees ◽  
Heavy Metal Contamination ◽  
Soil Samples ◽  
Control Group ◽  
Biotic Factors ◽  
Three Samples ◽  
Lead Zinc ◽  
Cadmium Lead ◽  
Bee Colonies

Проблема и цель. Целью научного исследования является изучение влияния антропогенных и биотических факторов на популяцию медоносной пчелы Рязанской области. Методология. Объект исследования: пчелиные семьи пасек разных районов Рязанской области, продукты пчеловодства. Для исследования были выбраны четыре участка пасеки, расположенные в границах Рязанской области. Опытные группы (далее ОГ) сформировали следующие участки: Кадомский район, д. Большое Лунино (54°34'58'' N, 42°21'25'' E) – ОГ №1; Захаровский район, д. Фурмакино (54°31'18'' N, 38°57'59'' E) – ОГ №2; Рыбновский район, с. Алешня (54°65'23'' N, 39°34'44'‘ E) – ОГ №3. В качестве контрольной группы (далее КГ) выбрана экспериментальная пасека Рыбновского района, с. Булыгино (54°66'58'' N, 39°26'52'' E). Пробоподготовка осуществлялась в период начала активного медосбора, путем отбора трех образцов каждого природного объекта. Образцы медоносных пчел отбирались от 5 пчелиных семей с каждого исследуемого участка, а также проводился отбор проб воды, медоносных растений и почвы. Для анализа степени пораженности заболеваниями нозематоз и варроатоз от каждой исследуемой пчелиной семьи было отобрано по 50-60 рабочих пчел. Результаты. Проведен сравнительный анализ на содержание токсичных металлов в образцахводы, почвы, теле медоносной пчелы, медоносных растений, пыльцевой обножки. Полученные данные свидетельствуют о том, что содержание кадмия, свинца, цинка и меди в образцах воды, медоносных растений, пыльцевой обножки и теле медоносных пчел находятся в предельно допустимой концентрации. Анализ образцов почвы показал превышение допустимой концентрации во всех исследуемых районах, за исключением Кадомского. Заключение. Загрязнение тяжелыми металлами прослеживается в цепочке: почва – медоносные растения – медоносная пчела – продукты пчеловодства в образцах Захаровского р-на, д. Фурмакино. В связи с этим размещение пасек в местах с повышенным содержанием токсичных элементов в природной среде может создавать угрозу интоксикации медоносных пчел и заноса этих металлов в продукты пчеловодства. Problem and goal. The purpose of the research is to study the infuence of anthropogenic and biotic factors on the population of the honey bee of the Ryazan region. Methodology. Object of research: bee families of apiaries of diferent districts of the Ryazan region, bee products. Four apiary plots located within the boundaries of the Ryazan Region were selected for the study. The experimental groups (hereinafter referred to as OG) formed the following sections: Kadomsky district, Bolshoe Lunino village (54°34 '58" N, 42°21'25" E) – OG No. 1, Zakharovsky district, Furmakino village (54°31'18" N, 38°57'59" E) – OG No. 2, Rybnovsky district, Aleshnya village (54°65'23" N, 39°34'44" E) - OG No. 3. As a control group (hereinafter referred to as the KG), the experimental apiary of the Rybnovsky district, p. Bulygino (54°66'58" N, 39°26'52" E). Sample preparation was carried out during the beginning of the active honey collection, by selecting three samples of each natural object. Samples of honey bees were taken from 5 bee colonies from each study site, as well as water, honey plants and soil samples. To analyze the degree of infection with nosematosis and varroatosis from each studied bee family, 50-60 worker bees were selected. Results. A comparative analysis of the content of toxic metals in samples of water, soil, honeybee body, honey plants, pollen pollen was carried out. The data obtained indicate that the content of cadmium, lead, zinc and copper in the samples of water, honey plants, pollen pollen and honey bees are in the maximum permissible concentration. The analysis of soil samples showed an excess of the permissible concentration in all the studied areas, with the exception of Kadomsky. Conclusion. Heavy metal contamination can be traced in the chain: soil-honey plants – honey bee-bee products in the samples of Zakharovsky district, Furmakino village. In this regard, the placement of apiaries in places with a high content of toxic elements in the natural environment can pose a threat to the intoxication of honey bees and the introduction of these metals into beekeeping products.

scholarly journals DYNAMICS OF HAEMATOLOGICAL AND FUNCTIONAL PARAMETERS OF PEKIN DUCK BLOOD DURING ADMINISTRATION OF ORGANIC SELENIUM PREPARATION

2018 ◽  
pp. 64-68
Author(s):  
Ye. O. Anisimova ◽  
V. V. Pronin ◽  
L. V. Kletikova ◽  
N. N. Yakimenko
Keyword(s):  
Total Protein ◽  
Color Index ◽  
Mineral Metabolism ◽  
Pekin Duck ◽  
Control Group ◽  
Axillary Vein ◽  
Functional Parameters ◽  
Organic Selenium ◽  
Age Related ◽  
Experimental Group

Age-related dynamics of hematological and functional parameters of Pekin duck blood during administration of organic selenium preparation DAFS-25k (at a 1.3 mg/kg dose) was presented. In order to study morphological parameters from the age of one day old to 120 days old blood was collected from axillary vein of birds prior to feeding each 15 days in the morning. To assess hematological parameters RBC and WBC count was performed, hemoglobin contents and hematocrit value were determined and color index of blood was calculated. The experiment demonstrated gradual increase in these parameters in the experimental and control groups. Birds from the experimental group demonstrated higher parameters than those of the control group. It was determined that hemoglobin contents was 9.86% higher in the experimental group in comparison with the control group due to selenium administration. The blood color index in this group was also higher (р ≤ 0.05). Biochemical blood analysis included the following parameters: total protein, albumin, uric acid, glucose, calcium, phosphorus, ALT and AST transaminase. The test results showed that during all age periods total protein and albumin contents in ducks of the experimental group exceeded those of the control group. The selenium-containing preparation had a positive influence on the carbohydrate metabolism ensuring high level of metabolic processes in birds, contributed to optimal calcium to phosphorus ratio which positively influenced mineral metabolism and development of the locomotor system. Obtained AST and ALT values in both groups are not beyond reference values which indirectly confirms absence of toxic effect on ducks’ organism if selenium is administered at the specified doses. It was determined that DAFS-25k has not affected hematological and functional parameters of Pekin duck blood and it contributed to mineral metabolism correction, reduction of stress factor effect within critical periods of development and improvement of the excretory system function.

scholarly journals Biological Evaluation of Wheat-Salty Extract, Milk-Wheat Solution and Fermented Soymilk for Treatment of Castor-Oil Induced Diarrhea in Rats

2012 ◽  
Vol 1 (1) ◽  
pp. 298 ◽  
Author(s):  
Ghada M. Khiralla ◽  
Safaa A. Salem
Keyword(s):  
Castor Oil ◽  
Biochemical Parameters ◽  
Positive Control ◽  
Biological Evaluation ◽  
Control Group ◽  
Font Size ◽  
Sodium Potassium ◽  
Functional Parameters ◽  
Potassium Magnesium ◽  
Fermented Products

<span style="font-family: Times New Roman; font-size: small;"> </span><p>Functional food or medicinal food is any healthy food claimed to have a health-promoting or disease-preventing property beyond the basic function of supplying nutrients. Three nutritional preparations including wheat powder salt solution (WPSS), milk-wheat solution (MWS), fermented milk (FM) and fermented soymilk (FSM) were evaluated for their anti-diarrheal activity by oral administration in model of Castor oil induced diarrhea in rats. Oral rehydration solution (ORS) was used as positive control. The fermented products were prepared using a mixture of <em>Lactobacillus acidophilus</em> ATCC 4356: and <em>Bifidobacterium bifidum</em> ATCC 700541 (1:1 v/v) to obtain a final level of 10<sup>7-8</sup> CFU/ml after incubation at 37°C. Beside the gain body weight (BW), certain biochemical parameters such as total protein, albumin, globulin, urea, creatinine, alanine amino-transferase (ALT), aspartate amino-transferase (AST), sodium, potassium, magnesium, iron and phosphorus were determined.</p> <p>According to follow the diarrheal symptoms including stool frequency, stool characteristics and BW, rats administrated with FSM were recovered from diarrhea (on the 3<sup>rd</sup> day) faster than other groups followed by those subjected with FM and CY. The ORS-positive control group rats were recovered on the 6<sup>th</sup> day, while diarrheal symptoms still appeared on the negative control rats (subjected with basal diet only; without ORS) with 16% death rate. Minerals, especially sodium, potassium, magnesium and phosphorus, were the most significant biochemical parameters for following recovery from diarrhea. The normal levels of these minerals were recovered in the blood serum at the end of experiment in rats administrated with the fermented products (FSM, FM and CY). Some renal functional parameters were suggested to follow diarrhea, but all studied liver functional parameters were not significantly recommended.</p><span style="font-family: Times New Roman; font-size: small;"> </span><span style="font-family: Times New Roman; font-size: small;"> </span>

scholarly journals Genetic and biochemical aspects of formation of professional chronic dust bronchitis

2019 ◽  
pp. 342-347
Author(s):  
A. S. Kazitskaya ◽  
N. I. Panev ◽  
T. K. Yadykina ◽  
O. N. Gulyaeva ◽  
N. A. Evseeva
Keyword(s):  
Respiratory System ◽  
Coal Industry ◽  
Main Group ◽  
The Body ◽  
Control Group ◽  
Immunological Parameters ◽  
Actual Problem ◽  
Functional Parameters ◽  
Increased Risk ◽  
The Impact

Introduction. Th e actual problem of modern occupational health is the study of the role of exogenous and endogenous factors in the development of occupational pathology of the bronchopulmonary system. То identify groups at increased risk of developing a dusty pathology of the bronchopulmonary system, it is necessary to conduct a comprehensive study of clinical and genetic factors, as well as to determine the most signifi cant diagnostic markers of the development of this pathology.The aim of the study was to study the genetic status of a set of biochemical and molecular genetic markers, as well as biochemical parameters of blood and respiratory function in coal industry workers with chronic dust bronchitis and persons of the control group.Materials and methods. 115 workers of coal mines from the South of Kuzbass aged from 39 to 58 years were examined in the Clinic of the Institute. Among them — 71 people with a previously established diagnosis of chronic dust bronchitis (the main group) and 44 people of the control group of persons working in the same sanitary conditions, but not having this pathology. A complex of clinical, biochemical and genetic methods of research was used in the study, and functional parameters of the bronchopulmonary system were evaluated. Statistical processing of the results was carried out using IBM SPSS Statistics 22 soft ware. Results. Statistically signifi cant diff erences between biochemical (increase in the concentration of ceruloplasmin and α–1antitrypsin) and immunological parameters (increase in the total number of leukocytes and ESR, increase in the concentration of IgG) in miners with chronic dust bronchitis and coal industry workers without this pathology were revealed. The dependence of the functional changes of the respiratory system with the development of professional pathology is determined. Th e persons of the main group showed a statistically signifi cant decrease in functional parameters (forced exhalation per second and lung capacity), increased respiratory failure. A predisposition to the development of dust bronchitis in the owners of the HP 1–1 genotype and resistance to the formation of this pathology in persons with the HP 2–2 genotype were found. Th e study of GSTT 1 deletion polymorphism revealed that carriers of the GSTT 1 «+» variant are most susceptible to the development of chronic dust bronchitis, and owners of the GSTT 1 variant are» resistant to its formation. Th ere was a positive аssociation with the development of dust bronchitis of the holders of the MM phenotype (MN).Conclusions. When working in similar conditions, some workers have a deviation of biochemical and immunological parameters fr om the norm, as well as a violation of the respiratory system, while others have no changes. Th e response of the body to the impact of certain external adverse factors may be due to genetic predisposition or resistance to the development of the disease. 

scholarly journals Ciliary function of the nose in patients with Osler`s disease and the effect of topically applied estrogens as a nose ointment

2011 ◽  
Vol 49 (4) ◽  
pp. 407-412
Author(s):  
J.U. Sommer ◽  
B.A. Stuck ◽  
C. Heiser ◽  
S.S. Kassner ◽  
K. Hormann ◽  
...  
Keyword(s):  
High Speed ◽  
Beat Frequency ◽  
Digital Camera ◽  
Ciliary Beat Frequency ◽  
Control Group ◽  
Mucus Clearance ◽  
Ciliary Function ◽  
The Mean ◽  
Positive Effect ◽  
Ciliary Beat

Background: In recent years, the positive effect of topically applied estriol nose ointment in the adjuvant therapy of Morbus-Rendu-Osler (HHT) has been proven. Due to the induced metaplasia, a complete destruction of the ciliated cells may be expected. However, data regarding the ciliary function of HHT patients with and without the use of topical estriol application are currently lacking. Methodology/principal: Ciliated samples were obtained by gently brushing the inferior nasal turbinate of 19 healthy volunteers and 15 patients with known HHT (8 of them regularly using 0.1% estriol nose ointment for 2 years (HHTwE) and 7 of them not using the ointment in the last 12 months (HHTwoE)). Analysis was done with an inverted phase contrast microscope connected to a high-speed digital camera. Recorded parameters were the visual integrity (VI) of the ciliary beat and its frequency (CBF) in Hz. Results: The VI index of all samples showed an undisrupted, even beating pattern with a difference between the three groups. The mean CBF in all HHT patients was reduced compared to the control group`s mean CBF. Within the HHT group itself, the mean CBF was reduced in the HHTwE group compared to the HHTwoE group. Conclusions: The ciliary beat frequency of HHT patients is impaired compared to the control group and even more so if the HHT patients topically apply estriol more than 6 months. An undisrupted beating pattern is found in the HHTwE group despite the fact that estrogens induce a transformation of the ciliated columnar into a keratinizing squamous epithelium. This data may justify the adjuvant application of estriol as a nose ointment in the treatment of epistaxis in HHT patients without the fear of damage to the nose`s mucus clearance.

scholarly journals CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia

2021 ◽  
Vol 7 (1) ◽  
pp. 00792-2020
Author(s):  
Pedro Sampaio ◽  
Mónica Ferro da Silva ◽  
Inês Vale ◽  
Mónica Roxo-Rosa ◽  
Andreia Pinto ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Open Source ◽  
Primary Ciliary Dyskinesia ◽  
High Speed ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Control Group ◽  
Observation Method ◽  
Ciliary Dyskinesia ◽  
Ciliary Beat

Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers.Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients.The correlation coefficient between the two methods was R2=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18–9.17 Hz at 25°C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93 Hz and in the PCD group (n=18), CBF ranged from 0 to 14.30 Hz.We offer an automated open-source programme named CiliarMove, validated by the manual observation method in a healthy volunteer control group, a PCD-excluded group and a PCD-confirmed group. In our hands, comparisons between CBF intervals alone could discern between healthy and PCD groups in 78% of the cases.

Sign in / Sign up

Export Citation Format

Share Document