The m6A reader IGF2BP3 Facilitates Gastric Cancer Progression Through Activating EMT via Upregulating MYC mRNA Stability

Background: N6-methyladenosine (m6A) RNA methylation plays an important biological role in cancer progression. Even so, the role of m6A modification in gastric cancer (GC) still needs further research. Methods: Firstly, based on the bioinformatics databases and human GC tissues analysis. Secondly, the IGF2BP3 expression in GC cells was measured by the quantificational real-time polymerase chain reaction and Western Blot. Then, the IGF2BP3 knockdown stable cells model was successfully constructed with the specific lentivirus-mediated short-hairpin RNA to explore the functions and mechanism of IGF2BP3 in GC. Next, the functions of IGF2BP3 on the cell phenotypes, including proliferation, invasion, migration, and Epithelial-mesenchymal transition process were clarified by the Cell Counting Kit-8, transwell, and WB experiments. Subsequently, RNA Immunoprecipitation analysis and mRNA stability experiments were used to verify the relationship between IGF2BP3 and MYC. Finally, in the rescue experiment, MYC was overexpressed and transfected into IGF2BP3 knockdown cells to further detect the influences on the cell phenotypes and the EMT process.Results: IGF2BP3 was up-regulated in GC. Meanwhile, IGF2BP3 had diagnostic and prognosis values for GC. Functionally, knockdown IGF2BP3 repressed gastric cancer cells proliferation, migration invasion and EMT process. Mechanically, IGF2BP3 activated the EMT process by improving the expression of MYC via combining with MYC mRNA and promoting its stability. Conclusions: Taken together, IGF2BP3 could activate the EMT process via increasing the MYC mRNA stability and expression to promote GC development, which provided insight into promising early diagnose and treatment for gastric cancer.

Role of Cardiac Biomarkers in Cancer Patients

In patients with cancer—and especially some specific subtypes—the heart can be pathologically affected due to the direct action of the tumor or its secretion products or due to the toxicity of some oncological treatments. Cardiac biomarkers have been investigated as inexpensive and easily accessible tools for prediction, early diagnosis, monitoring, or prognosis of various forms of cancer-related cardiac diseases. However, their clinical usefulness was not always clearly demonstrated in every area of cardioncology. For the identification of anthracycline related cardiotoxicity in the very early stages troponins proved to be more efficient detectors than imaging methods. Nevertheless, the lack of a standardized dosage methodology and of cardiotoxicity specific thresholds, do not yet allow to outline the precise way to employ them in clinical routine and to incorporate them into appropriate diagnostic or managing algorithms. Cardiac biomarkers proved also effective in patients with primary cardiac amyloidosis, in which both troponins and natriuretic peptides were able to predict adverse outcome, and carcinoid heart disease, where a precise diagnostic cut-off for N-terminal prohormone of brain natriuretic peptide (NT-proBNP) was identified to screen patients with valvular involvement. Likewise, NT-proBNP proved to be an excellent predictor of postoperative atrial fibrillation (POAF). On the contrary, evidence is still not sufficient to promote the routine use of cardiac biomarkers to early diagnose myocarditis due to immune check points inhibitors (ICIs), radiotherapy induced cardiotoxicity and cardiac complications related to androgenetic deprivation. In this review we present all the evidence gathered so far regarding the usefulness and limitations of these relatively inexpensive diagnostic tools in the field of cardio-oncology.

Post-Traumatic Hydrocephalus - A Retrospective Study

BACKGROUND Post-traumatic hydrocephalus (PTH) can occur as an outcome of moderate and severe traumatic head injury. It is one of the causes of delayed worsening of the early symptoms of head injury. A total of 18 cases of PTH diagnosed and treated at tertiary care hospitals from 2012 to 2015 were studied. The purpose of the study was to evaluate the clinicoradiological profile of patients diagnosed with posttraumatic hydrocephalus and determine the outcome of ventriculoperitoneal (VP) shunting in cases of post-traumatic hydrocephalus. METHODS A retrospective study was conducted in the Department of Neurosurgery in a teaching medical college. The clinicoradiological profile of patients diagnosed with PTH was studied. These cases were treated conservatively as well as by surgery. The demography, cause of trauma, GCS score and response rates were studied. RESULTS Among the 18 reviewed patients, 61.1 % were males, fall and RTA constituted the major cause of trauma. Craniotomy was done in 50 % of the patients, 88.89 % of the patients recovered and the mortality rate was 11.11 %. CONCLUSIONS Post-traumatic hydrocephalus is a consequence of traumatic head injury and can occur with various neurological symptoms after the initial trauma. CT scan of the brain is considered the choice of investigation to early diagnose PTH and the patient outcome after VP shunting is good. KEY WORDS Hydrocephalus, Head Injury, Trauma

Odontogenic Keratocyst

Purpose: to review in detail various aspects of odontogenic keratocyst, emphasizing recent nomenclature, clinical, histopathological, recurrence, and management of odontogenic keratocyst. Methods: To achieve the objective of this review, a manual search was done in hard copy books of oral and maxillofacial pathology, and an electronic search was done in the google website, oral and maxillofacial pathology E-books, virtual database sites, such as PubMed, Research Gate, Academia, and Google scholar using the descriptors: odontogenic cyst, kerato odontogenic tumor, odontogenic keratocyst, and jaws cystic lesion. The eligibility criteria for selecting articles were: to be in the English language, studies published in journals, or indexed in these databases until 2021. Exclusion criteria were: articles in any languages other than English, studies presented in duplicate between the bases, whose theme did not contemplate the objective proposed in this review, or those not available in the digital environment. Data collection occurred from October to December 2020, followed by a thorough evaluation of the studies found, including an exploratory, selective, analytical, and interpretative reading. Summary and conclusions: the odontogenic keratocyst is noteworthy because of its unusual growth pattern, the tendency to recur, and association with an inherited syndrome. The renaming of odontogenic keratocysts as keratocystic odontogenic tumors has been one of the most debatable changes in the terminology of odontogenic lesions in recent years. Early diagnose of this lesion is important to perform the more conservative treatment. A wait-and-see policy, with yearly follow-up for the first five years and every two years after that, is strongly advocated.

Diagnostic tool for the diagnosis of physiological and pathological conditions of the uterus in cows postpartum

Objective An important task of veterinarians in cattle husbandry is the early diagnosis of postpartum complications that can lead to infertility. The aim of the study was the evaluation of a new device to distinguish between physiological and pathological conditions of the uterus based on the determination of the time of uterine involution and the physicochemical properties of the vaginal discharge (lochia) during the first weeks after parturition. Material and methods A total of 173 black-and-white Holstein-Friesian dairy cows were examined for any pathological alteration of the uterine involution on days 1–2, 6–8, 11–14 postpartum and, in case of pathological findings, additionally on days 18–22, 23–27 and 28–30 after parturition. In addition to the standard examination a newly developed hand-held instrument (“Metrastatum”) was used to differentiate between physiological and pathological uterine involution. It allows determination of the distance between the cervix and vulva (DCV) and simultaneous collection of lochial secretions. Results The best time to use the new instrument was 10–16 days postpartum. In healthy cows without disturbed puerperium, the average DCV was 25.7 cm (± 0.8 cm) in this phase. This distance decreased by 12.5 ± 0.3 cm compared to the first days. In contrast, in cows with postpartum problems, the DCV was significantly greater at (31 ± 0.7 cm) (p < 0.001). In these animals, the AGV was reduced only by an average of 8.4 ± 0.6 cm when compared to the first days. The mucus obtained with the dome-shaped rubber funnel of the instrument also differed in the cows of the 2 groups. In healthy cows without puerperal problems, the lochia were thick, colorless, transparent and sometimes cloudy. In case of puerperal disorders, the discharge was thin, light red to reddish brown, contained flakes and had a nasty odor depending on the form of the inflammation. Conclusion The dual function of device helps to early diagnose puerperal disorders in cows and to select healthy animals for reproduction and sick cows for treatment depending on the form of uterine inflammation.

Leucoplasia: revisão de literatura e apresentação de caso clínico

Leucoplakia is a clinica' term that refers to an alteration on the oral mucosa with the appearance of white patchers that are firmly adhered and cannot be classified as any other diagnosable discase. They are considered premalignant lesions due to the increase in the risk of malignant transformation. In this case, around 4% to 6% of the lesions evolve to cancer. Then, it is necessary to estabilish a correct and early diagnose and also a precise treatment to reach good results. The periodical control of the patients that show this kind of alteration is extremely important. The aim of this study is to discuss the possible ethiological agents, clinical and histopathological aspects as well as, the treatment and prognoses through literature review, illustrating with a clinical case.

Oral verrucous carcinoma: a 24-year epidemiological study and case report

Introduction: Verrucous carcinoma is a non-metastatic variant of squamous cell carcinoma. It was first reported by Ackerman in 1948. It is a verrucous exophytic tumor and, although it is a rare lesion, it mainly affects the oral cavity, with slow growth and can be locally invasive. Objective: To report a case and present an observational and retrospective analysis of medical records containing biopsy data from patients diagnosed with oral verrucous carcinoma (OVC) in an oral diagnostic referral service for a period of 24 years. Material and methods: The following data were collected: age, sex, ethnicity, anatomical location and management. Result: Eight cases of OVC were found, all in Caucasian patients (n = 8, 100%), aged between 57 and 102 years. 62% of the injuries affected women. The most affected region was the jugal mucosa (n = 4, 50%). In addition, Smoking patients were not found. In all cases, incisional biopsy was performed and referral to the head and neck surgeon. Conclusion: Caucasian women in the sixth and seventh decades of life were the most affected by OVC. The absence of smoking patients corroborates the literature, which reports that cigarettes appear to be unrelated to this injury. The dentist's role is to recognize the disease early, diagnose it and refer it for medical treatment.

UPLC-MS/MS Determination of Linezolid and Heme in Plasma of Infected Patients and Correlation Analysis

Linezolid can cause serious haematological toxicity, such as thrombocytopenia and aneamia. Heme, composed of iron and porphyrin, is an important component of hemoglobin. In order to investigate the relationship between the concentration of linezolid and heme in the plasma of infected patients, a UPLC-MS/MS method that can determine the concentrations of linezolid and heme simultaneously was developed and validated. A total of 96 healthy subjects and 81 infected patients, who received blood routine blood tests, were included and determined by the UPLC-MS/MS method. The results showed that the concentration of linezolid was 5.08 ± 3.46 μ g / mL in infected patients who were treated with linezolid. The heme in healthy subjects was 7.05 ± 8.68 μ g / mL , and it was significantly decreased to 0.88 ± 0.79 μ g / mL in infected patients ( P < 0.01 ). Spearman correlation analysis showed that linezolid had a high negative correlation with platelet (PLT) ( R = − 0.309 ). Heme had a high positive correlation with hemoglobin (Hb) ( R = 0.249 ) in healthy subjects and infected patients. The ROC analysis showed that heme had diagnostic value to distinguish low Hb (110 g/L). In conclusion, there was a positive correlation between heme and Hb, and this correlation was also observed in infected patients. A high concentration of linezolid was inclined to decrease PLT. Monitoring of heme and linezolid helps in the early diagnose of low Hb and PLT.

COMPUTER VISION SYNDROME: REVIEW AND METHODS FOR ASSESSMENT

Computer vision syndrome (CVS) is a combination of eye and vision problems associated with the use of digital devices. CVS affects the eyesight, visual comfort and occupational productivity of workers due to the different symptoms computer users and staff experience: ocular discomfort, headache, dry eye, blurred vision etc. Aim of the study: To assess the symptoms of CVS in a software company in Varna by subjective and objective tests and prescribe measures for prevention. Materials and methods: 40 participants-31 men and 9 women were distributed with a valid questionnaire regarding common symptoms of CVS. The group was tested with the Schirmer test to measure the tear film quantity. Participants have passed a medical exam by an ophthalmologist before the study. Results: - 11 or 27,5% of the participants in the questionnaire have reached score ≥6 – have CVS - 10 or 25 % of the participants in the Schirmer test have reached results < 5mm. in the tear strip - The gender distribution shows 3 women (33.33%) and 8(25.8%) of men have CVS by questionnaire study and 3(33.33%) of women and 7(22.6%) of men based on Schirmer test have dry eye as asymptom of CVS. Conclusion: The results of the study predispose to further evaluation and tracking of the symptoms of CVS among this occupational group. All of the participants are aware of Ordinance № 7 related to health and safety work conditions with VDT’s and received information regarding the physiological work-rest regime. Occupational risk of CVS can be reduced with early diagnose, assessment and prevention of the symptoms.

Palato-gingival groove: A silent nidus. Recognition and an innovative management approach: A report of 3 cases

Developmental anomalies are one of the challenging clinical conditions to achieve successful treatment outcomes. Among these, Palatogingival groove [PGG] is a rare developmental anomaly usually found on the palatal aspect of maxillary anterior teeth. As self-cleansing is difficult in such patients, it can lead to serious localized periodontal lesion or can occur in combination with endodontic lesion. It is our sole responsibility being a specialist in the field of dentistry, to early diagnose and provide treatment for such conditions. On the basis of PGG extension to the pulp, it can be classified as simple or complex; accordingly, plethora of treatment options are available. Clinically taking interdisciplinary treatment modalities into consideration, curettage of the involved gingival tissues, elimination of the groove by grinding (saucerization), or by sealing with a Biocompatible material which might enhance the healing potential. Incase if the groove extends beyond the middle-third of the root, surgical flap debridement along with combined use of barrier membrane and alloplastic bone graft are recommended.This article represents a rare clinical case reports in management of PGG defect with an innovative conservative approach for simple, minimal groove extensive case and groove extension beyond 1/3 of the root region, treated with surgical multi-interdisciplinary approach.