A Comparison of Various Chips Used for the Manufacture of Biosensors Applied in Non-Fluidic Array SPRi, Based on the Example of Determination of Cathepsin D

Non-fluidic array SPR imaging (SPRi) with appropriate biosensors is a new tool for the determination of various biomarkers in body fluids. Numerous biomarkers can be determined without signal enhancement or preliminarily preconcentration. The introduction of a new material solution of the chip may increase the scope of the application of this technique. Solutions with adhesive separating foil and an Ag/Au chip were compared with the previously used two-paint separating polymer and pure gold chip. These solutions were tested using the example of a biosensor for cathepsin D (Cath D), which consisted of pepstatin A (a Cath D inhibitor) immobilized via a cysteamine linker using the NHS/EDC protocol. Four material versions of the Cath D biosensor proved adequate in terms of range of linearity, LOQ, precision and recovery. All four versions of the biosensor were used for the determination of Cath D in the blood serum patients with glioblastoma and control samples, producing very similar results and showing an elevated biomarker concentration in the case of cancer. Therefore, the problem of determining the correct level of Cath D in the serum of healthy individuals has been resolved, correcting literature data which ranged over three orders of magnitude.

Analysis of the Drag Flick Technique in the Indoor Hockey Game in the 2020 PON Papua Regional Youth Athletes in 2020

This study was to analyze the movement of the drag flick technique and to find out the correctness of the motion of the technique when dragging the flick on female athletes from the PON Papua 2020 plateaus. This study used a qualitative descriptive approach research design. By analyzing the motion of the drag flick technique for athletes who have participated in the National Training Program, which is used as a comparison and indoor hockey athletes from the Regional Plates of Prapon 2019 as athletes who are used as research samples using the Dartfish Teampro 5.5 software. This research was assisted by experts, namely one expert in the field of indoor hockey. The instrument used is Expert Judgment. The percentage of success that has been analyzed using Darfish software at the prefix position stage is very good (33%), Good (60%), Poor (6.6%), very poor (0%). the position of the foot crossover is very good (13%), Good (53.3%), Poor (33.3%), very poor (0%). the position of implementation is very good (22.2%), Good (50%), Poor (27.7%), very poor (0%). advanced motion positions are categorized as very good (0%), Good (34.7%), Less (65.2%), very poor (0%). The percentage of success based on expert judgment at the prefix position stage is Very Good (33%), Good (60%), Less (6.6%), Very Less (0%). foot crossover position is very good (13.3%), good (53.3%), less (33.3%), very poor (0%). the position of the implementation of the categories is Very Good (22%), Good (50%), Less (27.7%), Very Poor (0%). position ending in the category Very Good 34.7%), Good (65.2%), Less (0%), Very Less (0%). The results of the analysis of the overall movement of the dartfish software are at the level of correctness of the motion of the drag flick motion technique in the less category. The hockey sports expert's assessment shows that the overall motion at the correct level of motion of the drag flick technique is in the insufficient category so that there is still much to be improved.

247 Single-Photon Emission Computed Tomography (SPECT) In Predicting Localisation of Parathyroid Adenomas: A Closed-Loop Prospective Study

Aim to measure the sensitivity of SPECT in localising parathyroid adenomas particularly whilst attempting minimally invasive parathyroidectomy. Method 2-cycles prospective study correlating SPECT and operative findings was conducted. All patients underwent ultrasound and SPECT, those with concordant findings on both modalities underwent minimally invasive parathyroidectomy, whilst patients with discordant findings on both modalities had central neck exploration. Following operative findings, recommendations of changing the SPECT reporting method such as surgically relevant structures adjacent to the lesion, such as the thyroid, were implemented. Following which, a second prospective study assessed the outcome of change. Results In the first cycle, 20 patients underwent partial parathyroidectomy, either by open (n = 13), or minimally invasive (n = 7) approach. SPECT sensitivity was 85% (n = 17) in identifying the adenoma. Out of all histologically confirmed adenomas, SPECT identified all adenomas (n = 17) on the correct side and 41% (n = 7) on the correct level (superior vs. inferior parathyroid). In the second cycle following recommendation, 14 patients had surgery, 9 as minimally invasive and 5 as open approach. SPECT sensitivity was 79% (n = 11) for adenoma identification. Of all confirmed adenomas, SPECT identifying adenomas at the correct side increased to 91% (n = 10) and correct level to 91% (n = 10). Conclusions SPECT alone is not yet regarded as gold standard in parathyroid adenoma localisation. The study has demonstrated that feedback to the radiologist of the operative findings may enhance its usefulness, particularly in attempting to proceed with a minimally invasive parathyroidectomy.

A Survey on Trending Topics of Microservices

Microservice is an architectural style and a software development methodology. Microservices are used as a group of independent units with narrowly specified responsibilities that interact through well-described REST APIs. It is observed that the most challenging part in developing any application using microservice architecture is decomposing it into the correct level of granularity at design and run time, which requires good skills and domain knowledge. In this paper, some prominent topics in microservices analysis, such as determining the size and boundaries of microservices using various decomposition approaches and extraction of microservices from large monolithic applications have been discussed and analysed. Work pertaining to essential quality metrics required for a microservices-based system has also been surveyed. In this survey paper, we have identified how these topics are correlated and proposed some steps that might be beneficial in the transformation of monolithic applications into microservices.

STUDI EKSPLANATORI DAN KONFIRMATORI TENTANG PENYEMBAH YANG BENAR BERDASARKAN YOHANES 4:1-26 DI KALANGAN JEMAAT ARAS GEREJA NASIONAL SE-KOTA JEMBER

Worship is an action that is not foreign to humans in the sense that every human being basically commits an act of worship (homo adorance). So that basically every human being must worship something. Especially for people who are religious, of course they realize that there are people who exceed everything called God / ALLAH. It becomes a problem when sin enters humans, which causes a blurred image of Allah and results in an inaccurate / distorted object of worship that God should be something else. On the other hand, Allah gives freedom to humans to express their worship to Allah which makes people feel that the worship they have done is correct and even feel that the worship they do is the most correct.Jesus in John 4: 1-26 then provides the standard for being a true worshiper, that is, a worshiper who worships in spirit and in truth. This benchmark then becomes a benchmark for researchers to measure and examine the implementation of true worshipers based on John 4: 1-26 among the National Church level congregations throughout the city of Jember.This research uses quantitative research methods with survey and confirmatory methods consisting of the first, literature study or literature study as a basis for a true worshiper theory based on John 4: 1-26. Second, in the form of a statement survey or questionnaire made to identify the correct level of implementation of worshipers based on John 4: 1-26 among the National Church level congregations in Jember which was initially tested for validation by 4 doctors, then construct tests were carried out through orthogonal iterations. 30 samples, then 35 questionnaire items were submitted as the final test to 150 samples.

Improved prediction of behavioral and neural similarity spaces using pruned DNNs

Deep Neural Networks (DNNs) have become an important tool for modeling brain and behaviour. One key area of interest has been to apply these networks to model human similarity judgements. Several previous works have used the embeddings from the penultimate layer of vision DNNs and showed that a reweighting of these features improves the fit between human similarity judgments and DNNs. These studies underline the idea that these embeddings form a good basis set but lack the correct level of salience. Here we re-examined the grounds for this idea and on the contrary, we hypothesized that these embeddings, beyond forming a good basis set, also have the correct level of salience to account for similarity judgments. It is just that the huge dimensional embedding needs to be pruned to select those features relevant for the considered domain for which a similarity space is modeled. In Study 1 we supervised DNN pruning based on a subset of human similarity judgments. We found that pruning: i) improved out-of-sample prediction of human similarity judgments from DNN embeddings, ii) produced better alignment with WordNet hierarchy, and iii) retained much higher classification accuracy than reweighting. Study 2 showed that pruning by neurobiological data is highly effective in improving out-of-sample prediction of brain-derived representational dissimilarity matrices from DNN embeddings, at times fleshing out isomorphisms not otherwise observable. Pruning supervised by human brain/behavior therefore effectively identifies alignable dimensions of semantic knowledge between DNNs and humans and constitutes an effective method for understanding the organization of knowledge in neural networks.

DOES WORKING CAPITAL HAS AN IMPACT ON PROFITABILITY OF INDIAN STEEL INDUSTRY

Working capital has a major role in day to day business activities. It is simply defined as the excess amount of current assets over current liabilities. Working capital also means the fund which is needed for the operations which are taken place in a firm every day. So working capital is considered as a fund which revolves all the time. This revolving fund consists of continues conversion of cash and raw materials in to inventory, then inventory to sales or debtors and this debtors into cash or investment. The existing Working capital in the company is also commonly known as Net Working Capital or net current assets, is the excess if current assets over currentliabilities. But this is on a particular day i.e., 31st March’s position. While it is important to know whether the company really has correct level of WC which is required to them? For this purpose there is a need to find out the required working capital and its relation with the profitability. Thus, the research work has included measuring the impact of WC on profitability. Further there must be discussions with the accounting experts of the selected 10 steel companies to know what they feel about their desired level and actual level of working capital.

A perspective on wrong level, wrong side, and wrong site spine surgery

Background: Four of the most common “errors” in spine surgery include: operating on the wrong patient, doing the wrong procedure, performing wrong-level surgery (WLS), and/or performing wrong-sided surgery (WSS). Although preoperative verification protocols (i.e. Universal Protocol, routine Time-Outs, and using the 3 R’s (i.e. right patient, right procedure, right level/side)) have largely limited the first two “errors,” WLS and WSS still occur with an unacceptably high frequency. Methods: In 20 studies, we identified the predominant factors contributing to WLS/WSS; unusual/anatomical anomalies/variants (i.e. sacralized lumbar vertebrae. lumbarized sacral vertebra, Klippel-Feil vertebrae, block vertebrae, butterfly vertebrae, obesity/morbid obesity), inadequate/poor interpretation of X-rays/fluoroscopic intraoperative images, and failure to follow different verification protocols. Results: “Human error” was another major risk factor contributing to the failure to operate at the correct level/side (WLS/WSS). Factors comprising “human error” included; surgeon/staff fatigue, rushing, emergency circumstances, lack of communication, hierarchical behavior in the operating room, and failure to “speak up”. Conclusion: Utilizing the Universal Protocol, routine Time Outs, and the 3 R’s largelly avoid operating on the wrong spine patient, and performing the wrong procedure. However, these guidelines have not yet sufficiently reduced the frequently of WLS and WSS. Greater recognition of the potential pitfalls contributing to WLS/WSS as reviewed in this perspective should better equip spine surgeons to avert/limit such “errors” in the future.

Design and implementation of automatic phase changer

Simulation of an Automatic Phase Changer (APC) circuit was performed in this project. The simulation was carried out using Proteus 8. Automatic Phase Changer (APC) automatically changes the phase as the name suggests. In three phase power system 3 inputs of APC circuit are connected to three phases of the system and its three outputs are connected to three different loads. These three loads always need their normal rated voltage for proper operation. Results observed that if voltage of any phase goes below the nominal rating the loads may malfunction. Here, Automatic Phase Changer comes into action. When a phase voltage goes below its nominal rating, APC provides correct level of voltage to the load connected to that phase.

Design and implementation of automatic phase changer

Simulation of an Automatic Phase Changer (APC) circuit was performed in this project. The simulation was carried out using Proteus 8. Automatic Phase Changer (APC) automatically changes the phase as the name suggests. In three phase power system 3 inputs of APC circuit are connected to three phases of the system and its three outputs are connected to three different loads. These three loads always need their normal rated voltage for proper operation. Results observed that if voltage of any phase goes below the nominal rating the loads may malfunction. Here, Automatic Phase Changer comes into action. When a phase voltage goes below its nominal rating, APC provides correct level of voltage to the load connected to that phase.