scholarly journals Study on biological characteristics and biomass production of the green microalgae (Nannochloris atomus) isolated from Vietnam for the extraction of bioactive compounds

2021 ◽  
Vol 19 (3) ◽  
pp. 577-588
Author(s):  
Luu Thi Tam ◽  
Ngo Thi Hoai Thu ◽  
Nguyen Thi Minh Hang ◽  
Chau Van Minh ◽  
Dang Diem Hong
Keyword(s):  
Cell Density ◽  
Bioactive Compounds ◽  
Biomass Production ◽  
Morphological Characteristics ◽  
Biological Characteristics ◽  
Raw Material ◽  
Rrna Gene ◽  
Green Microalgae ◽  
High Biological Activity ◽  
Algae Biomass

Microalgae are known to be a nutrient-rich feed source for many aquatic animals. It is also an important raw material source to exploit high biological activity substances for humans. This is the first study on biological characteristics and algae biomass production from the green microalgae Nannochloris atomus being carried out in Vietnam. In this study, scientific name of the strain N. atomus NT12 based on morphological characteristics and 18S rRNA gene sequence (with accession number MW007766 on the GenBank) was identified. At the best conditions for the growth (i.e. Walne medium, 3 x 106 cells/mL initial cell density, 25 -30oC growth temperature, 60 - 100 µmol/m2s light intensity, pH 7, 30‰ salinity), highest NT12 strain cell density of 30 x 106 cells/mL was obtained after 30 days of culture. The microalgae N. atomus NT12 was also successfully cultured on a pilot scale in the plastic bottle 10 L and closed photobioreactors 20 – 50 L resulting in a high biomass productivity of 209 mg/L/day and a biomass rich in polyunsaturated fatty acids such as oleic acid (C18:1n-9), linoleic acid (C18:2n-6) and α-linolenic acid (C18:3n-3) qualified for the purpose of extraction of value bioactive compounds.

scholarly journals Discrimination of Myrtle Ecotypes from Different Geographic Areas According to Their Morphological Characteristics and Anthocyanins Composition

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 328 ◽  
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Estrella Espada-Bellido ◽  
Marta Ferreiro-González ◽  
Antonio Amores-Arrocha ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fruit Size ◽  
Morphological Characteristics ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Chemical Traits ◽  
Positive Effect

Myrtus communis L. is an evergreen shrub that produces berries with a high content in antioxidant compounds. Since these compounds have demonstrated a positive effect on human health, the interest on berries and their usages has increased. However, environmental conditions may affect the productivity of these species and consequently the quality of wild myrtle. Ecotypes from diverse geographical origins may result in significant variations in terms of bioactive compounds content as well as in chemical traits. For this reason, in this work ecotypes from two different localizations have been studied to determine if their differences in morphological and anthocyanins traits can be attributed to their origin and the environmental characteristics of these locations. For this, chemometric analyses such as Hierarchical Cluster Analysis and Principal Component Analysis, were employed. The results showed differences between the ecotypes depending on their location. In particular, myrtle berries from maritime zones present greater fruit size and amount of bioactive compounds, which means an improvement in the quality of the final product based on this raw material. It can be concluded that both morphological and anthocyanins traits are related to the location of the ecotype and allow selecting the best ecotype for the required applications.

scholarly journals Bioactive compounds in the aboveground part of hemiephemeroid onions (Allium L.)

2021 ◽  
Vol 181 (4) ◽  
pp. 37-43
Author(s):  
T. I. Fomina ◽  
T. A. Kukushkina
Keyword(s):  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Dry Matter ◽  
Matter Content ◽  
Raw Material ◽  
Dry Matter Content ◽  
High Biological Activity ◽  
Constant Weight ◽  
Total Sugars

Background. Representatives of the genus Allium L. are valuable food and medicinal plants that have long been used for nutrition and human health. Modern research has proved their high biological activity. Earlier, we investigated the aboveground organs of a number of wild onion species in the consumer ripeness phase. Higher content of secondary metabolites has been observed in the hemiephemeroid species A. aflatunense B. Fedtsch., A. microdictyon Prokh., and A. rosenbachianum Regel. The aim of this work was to determine the content of bioactive compounds in the green biomass of Allium species during flowering.Materials and methods. We studied the freshly harvested raw materials – leaves and flower scapes. Dry matter content was measured by drying 1 g of raw material at 100–105°C to constant weight. The amounts of phenolic compounds, pectic substances, total sugars and carotenoids were assessed spectrophotometrically using the SF-56 (Russia) and SF Agilent 8453 (USA) instruments, and ascorbic acid was measured by the titrimetric method. The result was taken as an average of three measurements for each indicator calculated on absolute dry weight of raw material (except ascorbic acid).Results and conclusions. The green biomass of Allium species was found to contain 8,2–16,2% of dry matter; 4,5–12,0% of phenolics (catechins, flavonols and tannins); 6,9–32,4% of total sugars; 9,5–12,6% of pectic compounds (pectins and protopectins); 20,8–102,5 mg% of carotenoids, and 38,0–197,7 mg% of ascorbic acid (wet weight). A significant range of variation in the content of secondary metabolites was due to the species’ characteristics and weather conditions of the growing season. A. microdictyon had a higher content of dry matter, flavonols and tannins, whereas A. aflatunense and A. rosenbachianum were rich in ascorbic acid and sugars. The findings testify to the prospects of using Allium spp. as a source of bioactive compounds. 

Study on biocharacteristics of Symbiodinium sp. isolated from soft coral in coastal water of Quang Nam, south central Vietnam

2017 ◽  
Vol 39 (3) ◽  
pp. 367-375 ◽  
Author(s):  
Dang Diem Hong ◽  
Pham Van Nhat ◽  
Hoang Thi Huong Quynh ◽  
Luu Thi Tam ◽  
Ngo Thi Hoai Thu ◽  
...  
Keyword(s):  
Cell Density ◽  
Coastal Water ◽  
Soft Coral ◽  
Morphological Characteristics ◽  
Acid Mixture ◽  
Rrna Gene ◽  
Bioactive Substances ◽  
Microalgal Biomass ◽  
South Central ◽  
Central Vietnam

Phototrophic dinoflagellates of the genus Symbiodinium (= zooxanthellae) are well known as symbionts of corals. They play an important role in maintaining the healthy as well as establishment of new reefs. In addition, products such as toxins or pigments from these symbiotic microalgae are potentially important for applications in the biomedical sciences, especially in new drug development. In this study, we isolated successfully a symbiotic microalga from soft coral Sinularia sp. which was collected in Cu Lao Cham coastal waters of Quang Nam province. Based on morphological characteristics and 18S rRNA gene sequences, isolated SHM8 strain was identified as Symbiodinium goreaui. This symbiont was cultured in different media such as ASP-A8, F2, ES, IMK, K, and L1. L1 culture medium supplemented with taurine concentration of 4.88 mM led to the cell density of SHM8 strain increased in 50% compared to control on day 2 in cultivation; amino acid mixture enhanced cell density by 31.7% on day 5 in cultivation. Supplementation of carbon source (bicarbonate salt of 16.8 g/L) and nitrogen source (nitrate salt of 0.055 g/L) enhanced the cell density by 40% and 27%, respectively on day 6 in culture. Obtained results in this study proved that this microalga can live independently without host. Successful isolation and cultivation of Symbiodinium microalga are one of the necessary conditions for promising researches on the natural bioactive substances from this microalgal biomass in the future in Vietnam.   Citation: Dang Diem Hong, Pham Van Nhat, Hoang Thi Huong Quynh, Luu Thi Tam, Ngo Thi Hoai Thu, Nguyen Cam Ha, Hoang Thi Lan Anh, Nguyen Hoai Nam, Nguyen Thi Minh Hang, Chau Van Minh, 2017. Study on biocharacteristics of Symbiodinium sp. isolated from soft coral in coastal water of Quang Nam, south central Vietnam. Tap chi Sinh hoc, 39(3): 367-375. DOI: 10.15625/0866-7160/v39n3.10112. *Corresponding author: [email protected] Received 19 June 2017, accepted 20 August 2017

Wastewater Treatment Using Marine Algae Biomass as Pollutants Removal

2018 ◽  
Vol 69 (5) ◽  
pp. 1089-1098
Author(s):  
Elena Suzana Biris Dorhoi ◽  
Maria Tofana ◽  
Simona Maria Chis ◽  
Carmen Elena Lupu ◽  
Ticuta Negreanu Pirjol
Keyword(s):  
Wastewater Treatment ◽  
Green Algae ◽  
Marine Algae ◽  
Tap Water ◽  
Ulva Lactuca ◽  
Raw Material ◽  
Bioactive Substances ◽  
Algae Biomass ◽  
Marine Biomass ◽  
Pollutants Removal

The valorification of the marine biomass is an important resource for many industries like pharmaceutical, supplying raw material for the extraction of bioactive substances (vitamins, sterols and collagen), cosmetics, biofertilizers and wastewater treatment. In the last years a special attention has been given to the use of macroalgae. The aim of this study was to emphasize the capacity of two representative green algae species frequent presents on the Romanian shore, Ulva lactuca (L.) and Cladophora vagabunda (L.) Hoek, to remove two usual detergents from wastewater. The green algae washed, dried at room temperature, macerated to powder were introduced into different filter paper for comparison, then immersed in waste water treated with different concentrations of detergents. Tap water was used for the experiment. The results show that Ulva lactuca (L.) species is suitable than Cladophora vagabunda (L.) Hoek species, for wastewater treatment.

scholarly journals The Influence of Temperature, Storage Conditions, pH, and Ionic Strength on the Antioxidant Activity and Color Parameters of Rowan Berry Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3786
Author(s):  
Elena Cristea ◽  
Aliona Ghendov-Mosanu ◽  
Antoanela Patras ◽  
Carmen Socaciu ◽  
Adela Pintea ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ionic Strength ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Acid Methyl Ester ◽  
Storage Conditions ◽  
Raw Material ◽  
Thermal Treatments ◽  
Spectrophotometric Methods ◽  
Berry Extracts

Recent trends in the food industry combined with novel methods in agriculture could transform rowan into a valuable raw material with potential technological applications. Thus, the aim of this research was to investigate the content of bioactive compounds in its fruits and to assess the color and antioxidant stability of the extracts prepared from such fruits during various thermal treatments and at different pH and ionic strength values. Various spectrophotometric methods, HPLC, and capillary electrophoresis were used to quantify the concentrations of bioactive compounds—polyphenols, carotenoids, organic acids, and to assess antioxidant activity and color. The results show that rowan berries contain circa 1.34–1.47 g/100 g of polyphenols among which include catechin, epicatechin, ferulic acid methyl ester, procyanidin B1, etc.; ca 21.65 mg/100 g of carotenoids including zeaxanthin, β-cryptoxanthin, all-trans-β-carotene, and various organic acids such as malic, citric, and succinic, which result in a high antioxidant activity of 5.8 mmol TE/100 g. Results also showed that antioxidant activity exhibited high stability when the extract was subjected to various thermal treatments, pHs, and ionic strengths, while color was mainly impacted negatively when a temperature of 100 °C was employed. This data confirms the technological potential of this traditional, yet often overlooked species.

scholarly journals Utilization of Cumbeba (Tacinga inamoena) Residue: Drying Kinetics and Effect of Process Conditions on Antioxidant Bioactive Compounds

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 788
Author(s):  
João Paulo de Lima Ferreira ◽  
Alexandre José de Melo Queiroz ◽  
Rossana Maria Feitosa de Figueirêdo ◽  
Wilton Pereira da Silva ◽  
Josivanda Palmeira Gomes ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Fixed Bed ◽  
Drying Kinetics ◽  
Raw Material ◽  
Process Conditions ◽  
Temperature Range ◽  
Water Diffusivity ◽  
Pharmaceutical Industries ◽  
Effective Water

The residue generated from the processing of Tacinga inamoena (cumbeba) fruit pulp represents a large amount of material that is discarded without proper application. Despite that, it is a raw material that is source of ascorbic acid, carotenoids and phenolic compounds, which are valued in nutraceutical diets for allegedly combating free radicals generated in metabolism. This research paper presents a study focused on the mathematical modeling of drying kinetics and the effect of the process on the level of bioactive of cumbeba residue. The experiments of cumbeba residue drying (untreated or whole residue (WR), crushed residue (CR) and residue in the form of foam (FR)) were carried out in a fixed-bed dryer at four air temperatures (50, 60, 70 and 80 °C). Effective water diffusivity (Deff) was determined by the inverse method and its dependence on temperature was described by an Arrhenius-type equation. It was observed that, regardless of the type of pretreatment, the increase in air temperature resulted in higher rate of water removal. The Midilli model showed better simulation of cumbeba residue drying kinetics than the other models tested within the experimental temperature range studied. Effective water diffusivity (Deff) ranged from 6.4890 to 11.1900 × 10−6 m2/s, 2.9285 to 12.754 × 10−9 m2/s and 1.5393 × 10−8 to 12.4270 × 10−6 m2/s with activation energy of 22.3078, 46.7115 and 58.0736 kJ/mol within the temperature range of 50–80 °C obtained for the whole cumbeba, crushed cumbeba and cumbeba residue in the form of foam, respectively. In relation to bioactive compounds, it was observed that for a fixed temperature the whole residue had higher retention of bioactive compounds, especially phenolic compounds, whereas the crushed residue and the residue in the form of foam had intermediate and lower levels, respectively. This study provides evidence that cumbeba residue in its whole form can be used for the recovery of natural antioxidant bioactive compounds, mainly phenolic compounds, with the possibility of application in the food and pharmaceutical industries.

scholarly journals Effect of Fertilisation on the Quality of Dried Coriander (Coriandrum sativum L.) and Lovage (Levisticum officinale)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 386
Author(s):  
Natalia Matłok ◽  
Józef Gorzelany ◽  
Adam Figiel ◽  
Maciej Balawejder
Keyword(s):  
Bioactive Compounds ◽  
Crop Production ◽  
Solid Phase ◽  
Quality Parameters ◽  
Raw Material ◽  
Vacuum Microwave ◽  
Dpph Method ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

The study presents the effects of fertilisation on selected quality parameters of the dried material obtained from plants of lovage and coriander. During the crop production process, the plants were treated with two fertilisers containing substances potentially acting as elicitors. The dried material was obtained in course of a drying process carried out in optimum conditions and based on the CD-VMFD method which combines convective pre-drying (CD) at a low temperature (40 °C) with vacuum-microwave finish drying with the use of 240 W microwaves (VMFD). The quality of the dried material was evaluated through measurement of the total contents of polyphenols, total antioxidant potential (ABTS and DPPH method), and the profile of volatile compounds (headspace-solid phase microextractio-HS-SPME) as well as assessment of the colour. It was found that by applying first fertilisation (with organic components) it is possible to significantly increase the contents of both bioactive compounds and volatile substances responsible for the aroma. It was determined that the higher content of bioactive compounds was related to the composition of the first fertiliser, presumably the extract from common nettle. The study showed that the application of the first fertiliser contributed to enhanced quality parameters of the raw material obtained.

scholarly journals Obtaining Bioactive Compounds from the Coffee Husk (Coffea arabica L.) Using Different Extraction Methods

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 46
Author(s):  
Mariana de Oliveira Silva ◽  
John Nonvignon Bossis Honfoga ◽  
Lorena Lucena de Medeiros ◽  
Marta Suely Madruga ◽  
Taliana Kênia Alencar Bezerra
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Extraction Methods ◽  
Water Bath ◽  
Raw Material ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ethanol Mixture ◽  
Coffee Husk ◽  
Coffea Arabica L

Coffee husks (Coffea arabica L.) are characterized by exhibiting secondary metabolites such as phenolic compounds, which can be used as raw material for obtaining bioactive compounds of interest in food. The objective of this study is to evaluate different methods for obtaining the raw material and extracting solutions of bioactive compounds from coffee husks. Water bath and ultrasound-assisted extraction methods were used, using water (100%) or ethanol (100%) or a mixture of both (1:1) as extracting solutions and the form of the raw material was in natura and dehydrated. The extracts were evaluated by their antioxidant potential using DPPH radicals, ABTS, and iron reduction (ferric reducing antioxidant power (FRAP)), and later total phenolic compounds, total flavonoids, and condensed tannins were quantified the phenolic majority compounds were identified. It was verified that the mixture of water and ethanol (1:1) showed better extraction capacity of the compounds with antioxidant activity and that both conventional (water bath) or unconventional (ultrasound) methods showed satisfactory results. Finally, a satisfactory amount of bioactive compounds was observed in evaluating the chemical composition (total phenolic compounds, total flavonoids, condensed tannins, as well as the analysis of the phenolic profile) of these extracts. Corroborating with the results of the antioxidant activities, the best extracting solution was generally the water and ethanol mixture (1:1) using a dehydrated husk and water bath as the best method, presenting higher levels of the bioactive compounds in question, with an emphasis on chlorogenic acid. Thus, it can be concluded that the use of coffee husk as raw material to obtain extracts of bioactive compounds is promising. Last, the conventional method (water bath) and the water and ethanol mixture (1:1) stood out among the methods and extracting solutions used for the dehydrated coffee husk.

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