Synergistic Effect of Lipid Hydroperoxyl Radical Scavenging and Lipid Hydroperoxide Reduction in the Inhibition of Lipid Peroxidation in Biomembranes

This study aimed to determine whether aerobic training could reduce lipid peroxidation and inflammation at rest and after maximal exhaustive exercise in overweight/obese adolescent girls. Thirty-nine adolescent girls (14-19 years old) were classified as nonobese or overweight/obese and then randomly assigned to either the nontrained or trained group (12-week multivariate aerobic training program). Measurements at the beginning of the experiment and at 3 months consisted of body composition, aerobic fitness (VO2peak) and the following blood assays: pre- and postexercise lipid peroxidation (15F2a-isoprostanes [F2-Isop], lipid hydroperoxide [ROOH], oxidized LDL [ox-LDL]) and inflammation (myeloperoxidase [MPO]) markers. In the overweight/obese group, the training program significantly increased their fat-free mass (FFM) and decreased their percentage of fat mass (%FM) and hip circumference but did not modify their VO2peak. Conversely, in the nontrained overweight/obese group, weight and %FM increased, and VO2peak decreased, during the same period. Training also prevented exercise-induced lipid peroxidation and/or inflammation in overweight/obese girls (F2-Isop, ROOH, ox-LDL, MPO). In addition, in the trained overweight/obese group, exercise-induced changes in ROOH, ox-LDL and F2-Isop were correlated with improvements in anthropometric parameters (waist-to-hip ratio, %FM and FFM). In conclusion aerobic training increased tolerance to exercise-induced oxidative stress in overweight/obese adolescent girls partly as a result of improved body composition.

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Biochemical Studies on Phenylhydrazine Induced Experimental Anemic Albino Rats

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v3i1.13258 ◽

2015 ◽

Vol 3 (1) ◽

pp. 41-47

Author(s):

Nirjala Laxmi Madhikarmi ◽

Kora Rudraiah Siddalinga Murthy

Keyword(s):

Lipid Peroxidation ◽

Lipid Hydroperoxide ◽

Thiobarbituric Acid ◽

Albino Rats ◽

Enzymatic Antioxidants ◽

Medical Sciences ◽

Thiobarbituric Acid Reactive Substances ◽

Biochemical Studies ◽

Healthy Control ◽

Wistar Albino Rats

INTRODUCTION: The present study evaluated the modulatory effects of diphenylhydrazine induced experimental wistar albino rats and also to assess various biochemical parameters in whole blood and red blood cell lysate.MATERIALAND METHODS: Twenty male albino rats weighing 180-200 gm were selected for the study and divided in two groups; ten phenylhydrazine dihydrochloride (PHZ) induced anemia and ten healthy control. Thiobarbituric acid reactive substances and lipid hydroperoxide were measured as lipid peroxidation parameter. The antioxidant vitamins A, C and E and enzymatic antioxidants; catalase, glutathione peroxidase and superoxide dismutase were also assessed.RESULTS: Phenylhydrazine induced anemic rats showed a significant increase in the lipid peroxidation and decrease in the antioxidants as compared to healthy rats.CONCLUSION: The study concludes that phenylhydrazine induced experimental anemic albino rats showed increased oxidative stress than compared with healthy albino rats.Journal of Universal College of Medical Sciences Vol. 3, No. 1, 2015: 41-47

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Investigation of Free Radical Scavenging Enzyme Activities and Lipid Peroxidation in Human Placental Tissues With Miscarriage

Journal of the Society for Gynecologic Investigation ◽

10.1016/j.jsgi.2006.04.003 ◽

2006 ◽

Vol 13 (5) ◽

pp. 384-388 ◽

Cited By ~ 16

Author(s):

Aydan Biri ◽

Mustafa Kavutcu ◽

Nuray Bozkurt ◽

Erdinç Devrim ◽

Nilhan Nurlu ◽

...

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Enzyme Activities ◽

Radical Scavenging ◽

Free Radical Scavenging

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The antioxidative-activity stability of aloe vera (Aloe vera var. chinensis) instant during storage

Food Research ◽

10.26656/fr.2017.5(3).052 ◽

2021 ◽

Vol 5 (4) ◽

pp. 288-293

Author(s):

Riyanto ◽

Ch. Wariyah

Keyword(s):

Lipid Peroxidation ◽

Critical Condition ◽

Antioxidative Activity ◽

Radical Scavenging ◽

Aloe Vera ◽

Oxygen Absorption ◽

Inlet Temperature ◽

Outlet Temperature ◽

Plastic Film ◽

Lipid Peroxidation Inhibition

Aloe vera contains a phenolic compound that has bioactive activity. Previous research showed that microencapsulation of aloe vera powder with maltodextrin as an encapsulation agent produced instant aloe vera with high antioxidative activity. The problem was the hygroscopic instant caused rapid moisture and oxygen absorption during storage, therefore decreasing the instant aloe vera antioxidative activity periodically. The aim of this research was to evaluate the antioxidative activity stability of instant aloe vera during storage. The processing of instant aloe vera through a reconstituted aloe vera powder with water with a ratio of 1:120 and then added with 2.5% maltodextrin as the encapsulating agent. The solution was then inserted into a spray dryer with an inlet temperature of 130oC, an outlet temperature of 103oC, and the flow rate of the solution is 350.0 mL/h. The resulted instant aloe vera was divided into 15 packs with a weight of 25 g, and each sample was wrapped with polyethylene plastic film with 0.80 mm thickness and then was stored at 25oC with a relative humidity of 75%. The sample was conducted in triplicate. The moisture content, and antioxidative activity that was based on the ability to capture 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical (RSA) and lipid peroxidation inhibition were analyzed every week until the critical condition was achieved at a moisture level of 12%. The research showed that the radical scavenging activity (RSA) and lipid peroxidation inhibition of instant aloe vera before storage were 16.34±1.22% and 39.33±1.68%, respectively, whereas in the critical condition the RSA was 3.63±0.04% and the lipid peroxidation inhibition was 22.31±0.02%. Based on their antioxidative activity, the appropriate storage time of instant aloe vera was about 12 weeks in polyethylene plastic film of 0.08 mm thickness

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Antioxidant and Antifungal Properties of Benzimidazole Derivatives

Zeitschrift für Naturforschung C ◽

10.1515/znc-2010-9-1002 ◽

2010 ◽

Vol 65 (9-10) ◽

pp. 537-542 ◽

Cited By ~ 3

Author(s):

Canan Kuş ◽

Fatma Sözüdönmez ◽

Benay Can-Eke ◽

Tülay Çoban

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Radical Scavenging ◽

Liver Microsomes ◽

Thiobarbituric Acid ◽

Benzimidazole Derivatives ◽

Rat Liver Microsomes ◽

Inhibitory Effects ◽

Stable Free Radical ◽

Radical Scavenging Properties

Antioxidant and radical scavenging properties of a series of 2-[4-(substituted piperazin-/ piperidin-1-ylcarbonyl)phenyl]-1H-benzimidazole derivatives were examined. Free radical scavenging properties of compounds 11-30 and 33 were evaluated for the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion radical. In addition the inhibitory effects on the NADPH-dependent lipid peroxidation levels were determined by measuring the formation of 2-thiobarbituric acid reactive substances (TBARS) using rat liver microsomes. Compound 33 which has a p-fluorobenzyl substitutent at position 1 exhibited the strongest inhibition (83%) of lipid peroxidation at a concentration of 10-3 M, while the nonsubstituted analogue 13 caused 57% inhibition. This result is fairly consistent with the antimicrobial activity results against both Staphylococcus aureus and Candida albicans.

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In vitro Antioxidant Activity and Inhibition of Fe2+ and SNP Lipid Peroxidation of African Mistletoes (Tapinanthus globiferus) from Three Selected Host Plants in Jos Plateau State Nigeria

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2019/v20i430090 ◽

2019 ◽

pp. 1-10

Author(s):

Jane-Rose I. Oche ◽

Titilayo O. Johnson ◽

Augustina O. Akinsanmi ◽

Kiri H. Jaryum ◽

Timothy Francis

Keyword(s):

Nitric Oxide ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Radical Scavenging ◽

Psidium Guajava ◽

Scavenging Activity ◽

Dpph Radical ◽

Vernonia Amygdalina ◽

Significant Difference ◽

Dpph Radical Scavenging

Aim: The aim of this study was to investigate and compare the antioxidative properties of the mistletoe plant obtained from three different host species namely Psidium guajava, Vernonia amygdalina and Moringa olifera lam. Study Design: Experimental Design Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences and Department of Biochemistry, College of Health Sciences, University of Jos, Nigeria. Methodology: Crude methanolic leaf extracts were studied for their antioxidative properties; Iron reducing and Iron-chelating activities, Nitric oxide (NO) radical and 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging activities and the lipid peroxidation and thiobarbituric acid reaction (TBAR) methods. One way ANOVA was used for the result analysis with P<.05 for significant difference. Results: Mistletoes from Psidum guajava (MSPG) had significantly higher reducing property (0.16 – 0.20mg/mL); the chelating property of Mistletoes from Moringa olifera (MSMO) was significantly lower (45.7 – 58.9%); DPPH radical scavenging activity had no significant difference; and Nitric oxide scavenging activity was significantly higher in MSPG (72.1% in 75mg/mL) than the extracts from other hosts. MSPG had significantly higher TBAR inhibition using both FeSO4 (77.8% at 125µg/mL) and Sodium nitroprusside (61.6+1.0% at 125µg/mL) with an IC50 of 30.27µg/mL . Extract of Tapinanthus globiferus leaves from Psidium guajava had more antioxidative activities in the TBARs followed by Tapinanthus globiferus leaf extract from Vernonia amygdalina (MSVA). Conclusion: From the study, mistletoes from Psidium guajava had higher antioxidant activity compared to other hosts, which probably justifies its use for treatment of cancer in traditional medicinal practice.

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Lipid Peroxidation as a Link Between Unhealthy Diets and the Metabolic Syndrome

10.5772/intechopen.98183 ◽

2021 ◽

Author(s):

Arnold N. Onyango

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Lipid Peroxidation ◽

Lipid Oxidation ◽

Saturated Fatty Acids ◽

Radical Scavenging ◽

The Metabolic Syndrome ◽

Mechanisms Of Formation ◽

Obesity Hypertension

Unhealthy diets, such as those high in saturated fat and sugar accelerate the development of non-communicable diseases. The metabolic syndrome is a conglomeration of disorders such as abdominal obesity, hypertension, impaired glucose regulation and dyslipidemia, which increases the risk for diabetes and cardiovascular disease. The prevalence of the metabolic syndrome is increasing globally, and dietary interventions may help to reverse this trend. A good understanding of its pathophysiological mechanisms is needed for the proper design of such interventions. This chapter discusses how lipid peroxidation is associated with the development of this syndrome, mainly through the formation of bioactive aldehydes, such as 4-hydroxy-2-nonenal, malondialdehyde, acrolein and glyoxal, which modify biomolecules to induce cellular dysfunction, including the enhancement of oxidative stress and inflammatory signaling. It gives a current understanding of the mechanisms of formation of these aldehydes and how dietary components such as saturated fatty acids promote oxidative stress, leading to lipid oxidation. It also outlines mechanisms, apart from free radical scavenging and singlet oxygen quenching, by which various dietary constituents prevent oxidative stress and lipid oxidation in vivo.

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Desiccation sensitivity and activities of free radical-scavenging enzymes in recalcitrant Theobroma cacao seeds

Seed Science Research ◽

10.1017/s0960258599000215 ◽

1999 ◽

Vol 9 (3) ◽

pp. 209-217 ◽

Cited By ~ 33

Author(s):

Changrun Li ◽

Wendell Q. Sun

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Water Content ◽

Theobroma Cacao ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Desiccation Sensitivity ◽

Critical Water Content ◽

Scavenging Enzymes ◽

Water Contents

AbstractMature and immature axes of Theobroma cacao (cocoa) seeds tolerated desiccation under a rapid-drying regime to critical water contents of 1.0 and 1.7 g g-1 dw, respectively. These critical water contents corresponded to water contents below which activities of free radical-scavenging enzymes (ascorbate peroxidase, peroxidase and superoxide dismutase) decreased rapidly during desiccation. The decline in axis viability below the critical water content was correlated with sharp increases in lipid peroxidation and cellular leakage. Cotyledon tissues were more desiccation-tolerant than axes, with a low critical water content of 0.24 g g–1dw. Desiccation sensitivity in cotyledon tissues was also correlated with the decrease in superoxide dismutase activity and increased lipid peroxidation products. However, in the cotyledons, no ascorbate peroxidase activity was detected at any water content, and peroxidase activity was gradually reduced as desiccation proceeded. Cocoa embryonic axes contained large amounts of sucrose, raffinose and stachyose but only traces of reducing monosaccharides. Desiccation sensitivity of recalcitrant cocoa axes did not appear to be due to the lack of sugar-related protective mechanisms during desiccation, and it was more likely related to the decrease of enzymic protection against desiccation-induced oxidative stresses.

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Potential antioxidant and lipid peroxidation inhibition of coffee mixed with lemongrass (Cymbopogon citrates) leaves

Nutrition & Food Science ◽

10.1108/nfs-01-2021-0036 ◽

2021 ◽

Vol ahead-of-print (ahead-of-print) ◽

Author(s):

Ayman El-Anany ◽

Sami Althwab ◽

Rehab Ali ◽

Rehab F.M. Ali ◽

Hassan Mousa

Keyword(s):

Lipid Peroxidation ◽

Health Benefits ◽

Radical Scavenging ◽

Control Sample ◽

Reducing Power ◽

Storage Period ◽

Dpph Radical ◽

Potential Health ◽

Content Type ◽

Different Levels

Purpose The purpose of this study is to evaluate the effect of the addition of dried lemongrass leaves (DLGL) powder, at different levels, on phenolics content, antioxidant activities, consumer acceptance and the inhibition of lipid peroxidation of roasted coffee (RC). Design/methodology/approach DLGL powder was incorporated at the levels of 0%, 2.5%, 5.0%, 7.5% and 10% of RC weight. The total flavonoids (TF), total phenolics (TP) and antioxidant activity measured using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reducing power assay of RC, DLGL and binary mixture of them determined. The oxidative indices of coffee oil samples during storage were investigated. In addition, the sensory characteristics of RC fortified with different levels of DLGL powder were evaluated. Findings The TP content of DLGL powder was 1,100.32 mg/100 g DWb, nearly 1.2 times higher than found in RC beans. The TF content of RC enriched with 2.5%, 5.0%, 7.5% and 10% DLGL were found to be around 1.05, 1.10, 1.15 and 1.20 times higher than that in the control coffee samples. RC supplemented with various levels of DLGL powder showed higher DPPH radical scavenging and reducing power activities. At the end of the storage period (six months), the acid, peroxide, P-Anisidine and total oxidation value values of RC supplemented with 10% DLGL powder were about 1.94, 2.52, 2.60 and 2.59 times as low as in the control sample without any addition of DLGL powder, respectively. RC containing 2.5% and 5.0% DLGL powder had significantly (p < 0.05) the highest sensory scores. Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits. Practical implications RC containing 2.5% and 5.0% DLGL powder had significantly (p = 0.05) the highest sensory scores. Originality/value Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits.

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Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts

Molecules ◽

10.3390/molecules25184055 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4055 ◽

Cited By ~ 1

Author(s):

Mariusz Banach ◽

Magdalena Wiloch ◽

Katarzyna Zawada ◽

Wojciech Cyplik ◽

Wojciech Kujawski

Keyword(s):

Lipid Peroxidation ◽

Interleukin 1 ◽

Radical Scavenging ◽

Water Soluble ◽

Anthocyanin Content ◽

Necrosis Factor Alpha ◽

Paramagnetic Resonance ◽

Markers Of Inflammation ◽

Radical Scavenging Properties

Aronia fruits contain many valuable components that are beneficial to human health. However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period. Therefore, there is a need to formulate the extracts with a precisely defined content of health-promoting substances. Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying. Subsequently, the content of anthocyanins, phenolic acids, and polyphenols was determined. The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards. Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations. Antioxidant and free-radical scavenging properties were evaluated using a 2,2′-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay. The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells. It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions. Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

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