Influence of carrier oil on the physical stability and in vitro digestion of vitamin K lipid nanocarriers

There is growing interest in developing biomaterial-coated liposome delivery systems to improve the stability and bioavailability of curcumin, which is a hydrophobic nutraceutical claimed to have several health benefits. The curcumin-loaded rhamnolipid liposomes (Cur-RL-Lips) were fabricated from rhamnolipid and phospholipids, and then chitosan (CS) covered the surface of Cur-RL-Lips by electrostatic interaction to form CS-coated Cur-RL-Lips. The influence of CS concentration on the physical stability and digestion of the liposomes was investigated. The CS-coated Cur-RL-Lips with RL:CS = 1:1 have a relatively small size (412.9 nm) and positive charge (19.7 mV). The CS-coated Cur-RL-Lips remained stable from pH 2 to 5 at room temperature and can effectively slow the degradation of curcumin at 80 °C; however, they were highly unstable to salt addition. In addition, compared with Cur-RL-Lips, the bioavailability of curcumin in CS-coated Cur-RL-Lips was relatively high due to its high transformation in gastrointestinal tract. These results may facilitate the design of a more efficacious liposomal delivery system that enhances the stability and bioavailability of curcumin in nutraceutical-loaded functional foods and beverages.

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Encapsulation of pterostilbene in nanoemulsions: influence of lipid composition on physical stability, in vitro digestion, bioaccessibility, and Caco-2 cell monolayer permeability

Food & Function ◽

10.1039/c9fo01260e ◽

2019 ◽

Vol 10 (10) ◽

pp. 6604-6614 ◽

Cited By ~ 3

Author(s):

Qianyuan Liu ◽

Jingjing Chen ◽

Yang Qin ◽

Bo Jiang ◽

Tao Zhang

Keyword(s):

Lipid Composition ◽

Cell Monolayer ◽

Physical Stability ◽

In Vitro Digestion ◽

Medium Chain Triglycerides ◽

Medium Chain ◽

Monolayer Permeability ◽

Long Chain Triglycerides ◽

Long Chain

Nanoemulsions fabricated using medium chain triglycerides as carrier lipid are more effective for delivering pterostilbene than long chain triglycerides.

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In-vitro digestion of high-oleic palm oil nanoliposomes prepared with unpurified soy lecithin: Physical stability and nano-liposome digestibility

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2019.123603 ◽

2019 ◽

Vol 578 ◽

pp. 123603 ◽

Cited By ~ 3

Author(s):

Juan David Beltrán ◽

Camilo Eduardo Sandoval-Cuellar ◽

Katherine Bauer ◽

María Ximena Quintanilla-Carvajal

Keyword(s):

Palm Oil ◽

Physical Stability ◽

In Vitro Digestion ◽

Soy Lecithin ◽

High Oleic

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Content and Bioaccessibility of Vitamin K (Phylloquinone and Menaquinones) in Cheese

Foods ◽

10.3390/foods10122938 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2938

Author(s):

Marie Bagge Jensen ◽

Andrius Daugintis ◽

Jette Jakobsen

Keyword(s):

Water Content ◽

Vitamin K ◽

Soluble Group ◽

Fermentation Process ◽

Starter Culture ◽

In Vitro Digestion ◽

Fat Content ◽

Ripening Time ◽

Cheese Products

Vitamin K is a fat-soluble group of vitamers consisting of phylloquinone (PK) and menaquinones (MKs). To date, only a daily reference intake for PK is set; however, in the last decade, research studying the correlation between MKs intake and improvement of health in regards to cardiovascular diseases, bone metabolism, and chronic kidney disease has been conducted. MKs are synthesised by bacteria in the fermentation process of foods, e.g., cheeses. The content and bioaccessibility of vitamin K vitamers (PK, MK-4, MK-5, MK-6, MK-7, MK-8, MK-9, and MK-10) were assessed in eight different cheese products differing in ripening time, starter culture, fat content, and water content. The bioaccessibility was assessed using the static in vitro digestion model INFOGEST 2.0. Variation of the vitamin K content (<0.5 μg/100 g–32 μg/100 g) and of the vitamin K bioaccessibility (6.4–80%) was observed. A longer ripening time did not necessarily result in an increase of MKs. These results indicate that the vitamin K content and bioaccessibility differs significantly between different cheese products, and the ripening time, starter culture, fat content, and water content cannot explain this difference.

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Probiotic fermented almond “milk” as an alternative to cow-milk yoghurt

International Journal of Food Studies ◽

10.7455/ijfs.v4i2.276 ◽

2015 ◽

Vol 4 (2) ◽

Cited By ~ 1

Author(s):

Neus Bernat ◽

Maite Cháfer ◽

Amparo Chiralt ◽

Chelo González-Martínez

Keyword(s):

Colloidal Stability ◽

Lactobacillus Reuteri ◽

Physical Stability ◽

Streptococcus Thermophilus ◽

In Vitro Digestion ◽

Fermented Milk ◽

Heat Treatments ◽

Cow Milk ◽

Demand For Health

Probiotics in almond-based matrices were considered as a means of obtaining fermented products which would cover both the current demand for health-promoting foods and for alternatives to standard yoghurts. Firstly, the combined effect of high pressure homogenisation (HPH) and heat treatment on the physical stability of almond “milk” was studied. The beverage was homogenised by applying 62, 103 and 172 MPa (MF1, MF2 and MF3 respectively); MF3 was also combined with two different heat treatments (85 ºC-30 min (LH) and 121 ºC-15 min (HH)). Both microstructure and colloidal stability were analysed in all the processed samples to select the most suitable treatment with which to obtain a stable product. The selected almond milk was then fermented with probiotic Lactobacillus reuteri and Streptococcus thermophilus and the final product was characterised throughout cold storage time (28 days) as to pH, acidity, serum retention and starter viability. A sensory evaluation and probiotic survival to in vitro digestion was also conducted. The results showed that the physical and structural almond-milk properties were affected by both HPH and heat treatments, obtaining the greatest stability in MF3-LH samples. The fermented milk permitted probiotic survivals above the level suggested as minimum for ensuring health benefits during the entire controlled time and, hence, can be considered as a functional food. No differences in the sensory acceptability of the product were found between 1 and 28 storage days. Therefore, a new, functional, fermented product was developed, which was suitable for targeted groups, such as the lactose-intolerant and cow-milk-protein allergic populations.

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Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action

Nanomaterials ◽

10.3390/nano11113035 ◽

2021 ◽

Vol 11 (11) ◽

pp. 3035

Author(s):

Teodora-Alexandra Iordache ◽

Nicoleta Badea ◽

Mirela Mihaila ◽

Simona Crisan ◽

Anca Lucia Pop ◽

...

Keyword(s):

Physical Stability ◽

Herbal Medicines ◽

Morphological Characteristics ◽

Entrapment Efficiency ◽

In Vitro Cytotoxicity ◽

Ongoing Research ◽

Lipid Nanocarriers ◽

Anti Inflammatory ◽

The Individual

There is ongoing research on various herbal bioactives and delivery systems which indicates that both lipid nanocarriers and herbal medicines will be fine tunned and integrated for future bio-medical applications. The current study was undertaken to systematically develop NLC-DSG-yam extract for the improved efficacy of herbal Diosgenin (DSG) in the management of anti-inflammatory disorders. NLCs were characterized regarding the mean size of the particles, morphological characteristics, physical stability in time, thermal behaviour, and entrapment efficiency of the herbal bioactive. Encapsulation efficiency and in vitro antioxidant activity measured the differences between the individual and dual co-loaded-NLC, the co-loaded one assuring a prolonged controlled release of DSG and a more emphasized ability of capturing short-life reactive oxygen species (ROS). NLCs safety properties were monitored following the in vitro MTS ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay) and RTCA (Real-Time Cell Analysis) assays. Concentrations less than 50 μg/mL showed no cytotoxic effects during in vitro cytotoxicity assays. Besides, the NLC-DSG-yam extract revealed a great anti-inflammatory effect, as the production of pro-inflammatory cytokines (TNF-alpha, IL-6) was significantly inhibited at 50 μg/mL NLC (e.g., 98.2% ± 1.07 inhibition of TNF-α, while for IL-6 the inhibition percentage was of 62% ± 1.07). Concluding, using appropriate lipid nanocarriers, the most desirable properties of herbal bioactives could be improved.

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Recent Advances on Nanoparticle Based Strategies for Improving Carotenoid Stability and Biological Activity

Antioxidants ◽

10.3390/antiox10050713 ◽

2021 ◽

Vol 10 (5) ◽

pp. 713

Author(s):

Kandi Sridhar ◽

Baskaran Stephen Inbaraj ◽

Bing-Huei Chen

Keyword(s):

Water Solubility ◽

Metal Oxide Nanoparticles ◽

Physical Stability ◽

Aqueous Solubility ◽

In Vitro Digestion ◽

Storage Conditions ◽

Lipid Nanoparticles ◽

Research Review ◽

Metal Metal

Carotenoids are natural pigments widely used in food industries due to their health-promoting properties. However, the presence of long-chain conjugated double bonds are responsible for chemical instability, poor water solubility, low bioavailability and high susceptibility to oxidation. The application of a nanoencapsulation technique has thus become a vital means to enhance stability of carotenoids under physiological conditions due to their small particle size, high aqueous solubility and improved bioavailability. This review intends to overview the advances in preparation, characterization, biocompatibility and application of nanocarotenoids reported in research/review papers published in peer-reviewed journals over the last five years. More specifically, nanocarotenoids were prepared from both carotenoid extracts and standards by employing various preparation techniques to yield different nanostructures including nanoemulsions, nanoliposomes, polymeric/biopolymeric nanoparticles, solid lipid nanoparticles, nanostructured lipid nanoparticles, supercritical fluid-based nanoparticles and metal/metal oxide nanoparticles. Stability studies involved evaluation of physical stability and/or chemical stability under different storage conditions and heating temperatures for varied lengths of time, while the release behavior and bioaccessibility were determined by various in vitro digestion and absorption models as well as bioavailability through elucidating pharmacokinetics in an animal model. Moreover, application of nanocarotenoids for various biological applications including antioxidant, anticancer, antibacterial, antiaging, cosmetics, diabetic wound healing and hepatic steatosis were summarized.

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Influence of β-Cyclodextrin and Hydroxypropyl-β-Cyclodextrin on Enhancement of Solubility and Dissolution of Isradipine

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2017.10.3.8 ◽

2017 ◽

Vol 10 (3) ◽

pp. 3752-3757

Author(s):

Narendar D ◽

Ettireddy S

Keyword(s):

Inclusion Complex ◽

Dissolution Rate ◽

Solid Dispersions ◽

Physical Stability ◽

Molecular Complexes ◽

In Vitro Dissolution ◽

Molar Ratio ◽

Phase Solubility ◽

Cyclodextrin Complexation

The content of this investigation was to study the influence of β-cyclodextrin and hydroxy propyl-β-cyclodextrin complexation on enhancement of solubility and dissolution rate of isradipine. Based on preliminary phase solubility studies, solid complexes prepared by freeze drying method in 1:1 molar ratio were selected and characterized by DSC for confirmation of complex formation. Prepared solid dispersions were evaluated for drug content, solubility and in vitro dissolution. The physical stability of optimized formulation was studied at refrigerated and room temperature for 2 months. Solid state characterization of optimized complex performed by DSC and XRD studies. Dissolution rate of isradipine was increased compared with pure drug and more with HP-β-CD inclusion complex than β-CD. DSC and XRD analyzes that drug was in amorphous form, when the drug was incorporated as isradipine β-CD and HP-β-CD inclusion complex. Stability studies resulted in low or no variations in the percentage of complexation efficiency suggesting good stability of molecular complexes. The results conclusively demonstrated that the enhancement of solubility and dissolution rate of isradipine by drug-cyclodextrin complexation was achieved.

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