Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live lactococci on the mammary immune response

2008 ◽  
Vol 75 (3) ◽  
pp. 374-384 ◽  
Author(s):  
Fiona Crispie ◽  
Mercedes Alonso-Gómez ◽  
Collette O'Loughlin ◽  
Katja Klostermann ◽  
James Flynn ◽  
...  
Keyword(s):  
Immune Response ◽  
Lactococcus Lactis ◽  
Acute Phase Proteins ◽  
Bovine Mastitis ◽  
Field Trials ◽  
Initial Field ◽  
Amyloid A ◽  
Probiotic Treatment ◽  
Post Infusion ◽  
Live Culture

In the accompanying article, we demonstrated that a live culture ofLactococcus lactiscompares favourably with antibiotics for treatment of bovine mastitis in two initial field trials. In an effort to explain the mechanism involved, this study investigated the effect of culture administration on the local immune response. In this respect we initially observed that infusion of the live cultureLactococcus lactisstimulated substantial recruitment of polymorphonucleocytes (PMN) and lymphocytes to the udder. For instance, in one assay, quarters infused with the probiotic experienced a dramatic increase (~20 000-fold) in neutrophils over the first 48-h period from an average value of 83·6 cells/ml pre-treatment to 1·78×106 cells/ml 48 h post-infusion. Levels of the acute phase proteins haptaglobin and milk amyloid A were also elevated significantly in comparison with controls following infusion of the culture. The results of flow cytometric assays also demonstrated that while infusion of a live lactococcal culture led to an enhanced recruitment of PMN to the udder (from 1·85×104 cells/ml pre-infusion to 1·45×106 cells/ml 24 h post-infusion) cell-free supernatant from the same culture was not able to do so, indicating that liveLc. lactiscan specifically trigger the mammary immune response to elicit PMN accumulation. These results suggest that the mechanism responsible for this probiotic treatment of mastitis is associated with stimulation of the host intramammary immune system.

scholarly journals Administration of a live culture ofLactococcus lactisDPC 3147 into the bovine mammary gland stimulates the local host immune response, particularlyIL-1β andIL-8gene expression

2009 ◽  
Vol 76 (3) ◽  
pp. 340-348 ◽  
Author(s):  
Christine Beecher ◽  
Mairéad Daly ◽  
Donagh P Berry ◽  
Katja Klostermann ◽  
James Flynn ◽  
...  
Keyword(s):  
Immune Response ◽  
Mammary Gland ◽  
Antibiotic Therapy ◽  
Bovine Mastitis ◽  
Field Trials ◽  
Immune Gene ◽  
Pronounced Increase ◽  
Post Infusion ◽  
Local Host ◽  
Live Culture

Mastitis is one of the most costly diseases to the dairy farming industry. Conventional antibiotic therapy is often unsatisfactory for successful treatment of mastitis and alternative treatments are continually under investigation. We have previously demonstrated, in two separate field trials, that a probiotic culture,Lactococcus lactisDPC 3147, was comparable to antibiotic therapy to treat bovine mastitis. To understand the mode of action of this therapeutic, we looked at the detailed immune response of the host to delivery of this live strain directly into the mammary gland of six healthy dairy cows. All animals elicited signs of udder inflammation 7 h post infusion. At this time, clots were visible in the milk of all animals in the investigation. The most pronounced increase in immune gene expression was observed inInterleukin(IL)-1β andIL-8, with highest expression corresponding to peaks in somatic cell count. Infusion with a live culture of aLc. lactisleads to a rapid and considerable innate immune response.

Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

2021 ◽  
pp. 1-5
Author(s):  
Aarsha Raj ◽  
Vinodkumar Kulangara ◽  
Tresamol P. Vareed ◽  
Deepa P. Melepat ◽  
Latha Chattothayil ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Solid Phase ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reactive Protein ◽  
Amyloid A ◽  
California Mastitis Test ◽  
Bacterial Organism

Abstract Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

scholarly journals The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response

2021 ◽  
Author(s):  
Łukasz Jarosz ◽  
Artur Ciszewski ◽  
Agnieszka Marek ◽  
Zbigniew Grądzki ◽  
Beata Kaczmarek ◽  
...  
Keyword(s):  
Immune Response ◽  
Acute Phase Proteins ◽  
Large White ◽  
Humoral Immune ◽  
Amyloid A ◽  
Group A ◽  
Pig Farm ◽  
Probiotic Microbes ◽  
Nonspecific Immune Response

AbstractThe aim of the study was to determine the effect of EM Bokashi® on selected parameters of the specific and nonspecific immune response of sows by in colostrum and milk samples. The percentage of cells with expression of CD19+, CD5+CD19+, CD21+, SWC3a (macrophage/monocyte), and CD11b+ molecules on the monocytes and granulocytes as well as the concentrations of lysozyme and acute phase proteins — serum amyloid-A (SAA) and haptoglobin (Hp) were evaluated. The study was carried out on a commercial pig farm, including 150 sows (Polish Large White × Polish Landrace) at the age of 2–4 years. Sixty female sows were divided into two groups: I — control and II — experimental. For the experimental group, a probiotic in the form of the preparation EM Bokashi® in the amount of 10 kg/tonne of feed was added to the basal feed from mating to weaning. The material for the study consisted of colostrum and milk. The samples were collected from all sows at 0, 24, 48, 72, 96, 120, 144, and 168 h after parturition. The study showed that exposure of the pregnant sow to the probiotic microbes contained in EM Bokashi® significantly affects the immunological quality of the colostrum and milk and caused an increase in the percentage of the subpopulations of B cells with CD19+, CD21+, and CD5+CD19+ expression in the colostrum and milk, which demonstrates an increase in the protective potential of colostrum and indicates stimulation of humoral immune mechanisms that protect the sow and the piglets against infections.

scholarly journals Patterns of serum immune biomarkers during elephant endotheliotropic herpesvirus viremia in Asian and African elephants

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0252175
Author(s):  
Katie L. Edwards ◽  
Erin M. Latimer ◽  
Jessica Siegal-Willott ◽  
Wendy Kiso ◽  
Luis R. Padilla ◽  
...  
Keyword(s):  
Immune Response ◽  
Acute Phase Proteins ◽  
Interleukin 2 ◽  
Underlying Disease ◽  
Hemorrhagic Disease ◽  
African Elephants ◽  
Necrosis Factor Alpha ◽  
Amyloid A ◽  
Immune Biomarkers ◽  
The Impact

Hemorrhagic disease (HD) caused by a group of elephant endotheliotropic herpesviruses (EEHV) is one of the leading causes of death for young elephants in human care. These viruses are widespread and typically persist latently in adult elephants with no negative effects; however, in juvenile Asian and more recently young African elephants, the onset of disease can be rapid and the mortality rate high. Measuring biomarkers associated with the immune response could be beneficial to understanding underlying disease processes, as well as the management of infection and HD. The goal of this study was to measure acute phase proteins and cytokines in serum collected from elephants infected with EEHV (13 Asian and 1 African) and compare concentrations according to presence, severity and outcome of disease. Serum amyloid A (SAA) and haptoglobin (HP) were higher in elephants with EEHV viremia than those without; concentrations increased with increasing viral load, and were higher in fatal cases compared to those that survived. In Asian elephants, SAA was also higher during EEHV1 viremia compared to EEHV5. Cytokine concentrations were typically low, and no statistical differences existed between groups. However, in individuals with detectable levels, longitudinal profiles indicated changes in tumor necrosis factor alpha (TNF-α) and interleukin-2 (IL-2) that may reflect an immune response to EEHV infection. However, the overall low concentrations detected using previously validated assays do not support the presence of a ‘cytokine storm’ and suggest more work is needed to understand if sub-optimal immune responses could be involved in disease progression. These results highlight the potential benefit of measuring circulating biomarker concentrations, such as APPs and cytokines, to improve our understanding of EEHV viremia and HD, assist with monitoring the progression of disease and determining the impact of interventions.

Intramammary infusion of a live culture ofLactococcus lactisfor treatment of bovine mastitis: comparison with antibiotic treatment in field trials

2008 ◽  
Vol 75 (3) ◽  
pp. 365-373 ◽  
Author(s):  
Katja Klostermann ◽  
Fiona Crispie ◽  
James Flynn ◽  
R Paul Ross ◽  
Colin Hill ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Bovine Mastitis ◽  
Clinical Signs ◽  
Experimental Period ◽  
Treated Group ◽  
Field Trials ◽  
Clinical Mastitis ◽  
Post Treatment ◽  
Cell Counts ◽  
Live Culture

A treatment containing a live food-grade organism,Lactococcus lactisDPC3147, was compared with conventional antibiotic therapy for its potential to treat bovine chronic subclinical or clinical mastitis in two separate field trials. Effects on disease symptoms and bacteriology were monitored in response to infusion with the culture in each trial. In the first trial, the live culture treatment was compared with an intramammary antibiotic (n=11 quarters for each treatment). Results from this small trial demonstrated that the live culture had potential to be as effective at eliminating chronic subclinical infections as an antibiotic treatment. By day 12, 7 of the 11 quarters treated with the live culture were pathogen-free compared with 5 of the 11 antibiotic-treated infected quarters. Somatic cell counts (SCC) remained relatively unchanged regardless of treatment: average log SCC pre- and post-treatment in the lactococci-treated group were 6·33±0·41 (day 0) and 6·27±0·43 cells/ml (day 12) and average log SCC pre- and post-treatment in the antibiotic-treated group were 6·34±0·37 and 6·22±0·46 cells/ml on day 0 and on day 12, respectively. In the second trial, the live culture was compared with an intramammary antibiotic for the treatment of naturally occurring clinical mastitis cases (n=25 quarters for each treatment). Following a 14-d experimental period, similar bacteriological responses were observed in 7 out of 25 live culture treated quarters and 9 out of 25 antibiotic-treated quarters. Additionally, 15 of 25 cases treated with the culture and 18 of 25 cases treated with the antibiotic did not exhibit clinical signs of the disease following treatment. The results of these trials suggest that live culture treatment withLc. lactisDPC3147 may be as efficacious as common antibiotic treatments in some instances.

scholarly journals Concentrations of Acute-Phase Proteins in Milk from Cows with Clinical Mastitis Caused by Different Pathogens

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 706
Author(s):  
Felipe M. Dalanezi ◽  
Elizabeth M. S. Schmidt ◽  
Sâmea F. Joaquim ◽  
Felipe F. Guimarães ◽  
Simoni T. Guerra ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Diagnostic Methods ◽  
Spatial Proximity ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Positive Correlations

Among the new diagnostic methods for mastitis detection under development, milk acute-phase proteins (APPs) are receiving special attention. The study aimed to compare the profile of milk APPs from cows with natural clinical mastitis caused by distinct pathogens. The concentrations of haptoglobin (Hp), serum amyloid A (SAA), alpha-1-acid glycoprotein (AGP), and C-reactive protein (CRP) were measured by Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL). Each APP was compared across the pathogens causing mastitis. The APPs differed statistically (p < 0.05) among the pathogens causing udder infection. There were significant and positive correlations among the concentration profile, for each pathogen, in three of four APPs studied. It can be concluded that the pathogen causing mastitis could modify the profile of release of the APPs in milk. The profile of Hp, AGP, and CRP demonstrated significant correlation, indicating that the three APPs are suggested as biomarkers, in milk, for bovine mastitis.

scholarly journals Patterns of serum immune biomarkers during elephant endotheliotropic herpesvirus viremia in Asian and African elephants

2021 ◽  
Author(s):  
Katie L Edwards ◽  
Erin Latimer ◽  
Jessica Siegal-Willott ◽  
Wendy Kiso ◽  
Luis Padilla ◽  
...  
Keyword(s):  
Immune Response ◽  
Acute Phase Proteins ◽  
Interleukin 2 ◽  
Hemorrhagic Disease ◽  
African Elephants ◽  
Necrosis Factor Alpha ◽  
Assay Sensitivity ◽  
Amyloid A ◽  
Immune Biomarkers ◽  
The Impact

Hemorrhagic disease (HD) caused by a group of elephant endotheliotropic herpesviruses (EEHV) is one of the leading causes of death for young elephants in human care. These viruses are widespread and typically persist latently in adult elephants with no negative effects; however, in juvenile Asian and more recently young African elephants, the onset of disease can be rapid and the mortality rate high. Measuring biomarkers associated with the immune response could be beneficial to understanding underlying disease processes, as well as the management of infection and HD. The goal of this study was to measure acute phase proteins and cytokines in serum collected from elephants infected with EEHV (13 Asian and 1 African) and compare concentrations according to presence, severity and outcome of disease. Serum amyloid A (SAA) and haptoglobin (HP) were higher in elephants with EEHV viremia than those without; concentrations increased with increasing viral load, and were higher in fatal cases compared to those that survived. In Asian elephants, SAA was also higher during EEHV1 viremia compared to EEHV5. Cytokine concentrations were typically low, and no statistical differences existed between groups, perhaps due to insufficient assay sensitivity in this age group. However, in individuals with detectable levels, longitudinal profiles indicated changes in tumor necrosis factor alpha (TNF-α) and interleukin-2 (IL-2) that may reflect an immune response to EEHV infection. These results highlight the potential benefit of measuring circulating biomarker concentrations, such as APPs and cytokines, to improve our understanding of EEHV viremia and HD, assist with monitoring the progression of disease and determining the impact of interventions.

PENGEMBANGAN BAHAN AJAR BERBASIS PENDEKATAN OPEN ENDED PADA MATERI FPB/KPK KELAS IV UNTUK MENINGKATKAN KEMAMPUAN PENALARAN SISWA DI SD NEGERI TAMBAKREJO 01 SEMARANG

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Ambar Widianingrum ◽  
Joko Sulianto ◽  
Rahmat Rais
Keyword(s):  
Data Analysis ◽  
Research And Development ◽  
Teaching And Learning ◽  
Analysis Data ◽  
Field Trials ◽  
Teaching Materials ◽  
Initial Field ◽  
Analysis Technique ◽  
Quantitative Descriptive ◽  
Stage 1

The purpose of this study was to describe the feasibility of teaching materials based on an open-ended approach to improve the reasoning abilities of fourth grade students in elementary schools. This type of research is research and development (Research and Development). The subjects of this study were 3 classroom teachers. The data analysis technique used is descriptive qualitative data analysis (data reduction, data presentation and conclusion) and quantitative descriptive data analysis. Based on the results of stage 1 media validation, it was obtained 84.8%, and the results of stage 2 media validation were obtained 94.8%. The result of material validation for stage 1 was obtained 84.6%, and validation for material for stage 2 was obtained 93.3%. The results of initial field trials obtained media 93.7% and material 92.3%. This shows that the teaching material is declared valid and suitable for use. Based on the results of this study, the suggestion that can be conveyed is that teaching materials based on an open-ended approach can be used as a tool for teaching and learning resources for students.

The Effect of Sedation, Oral Examination, and Odontoplasty on Systemic Inflammation as Measured by Serum Amyloid A in the Adult Performance Horse

2019 ◽  
Vol 36 (3) ◽  
pp. 198-201
Author(s):  
Sheri S. W. Birmingham ◽  
Rocky M. Mason
Keyword(s):  
Systemic Inflammation ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Systemic Disease ◽  
Oral Examination ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Adult Performance ◽  
Normal Limits ◽  
Time Periods

Serum amyloid A (SAA) is one of the major acute phase proteins in horses. It serves as a marker for systemic inflammation and infection, as the concentration can increase 100- to even 1000-fold during systemic disease processes. The objective of this study was to evaluate the effect of sedation, oral examination, and odontoplasty on systemic inflammation as measured by SAA in the adult performance horse. This study included 32 clinically healthy adult performance horses. Blood samples were collected immediately prior to sedation, oral examination, and odontoplasty and 48 and 72 hours afterward. Serum amyloid A levels were measured directly after venipuncture using a commercially available stall-side lateral flow immunoassay test developed and validated for equine SAA levels. Serum amyloid A values were within normal limits for each of the time periods and there were no significant differences in SAA values between the time periods. The results of this study suggest that sedation, oral examination, and odontoplasty have no systemic inflammatory effects as measured by SAA.

scholarly journals Effect of Foot-and-Mouth Disease Vaccination on Acute Phase Immune Response and Anovulation in Hanwoo (Bos taurus coreanae)

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 419
Author(s):  
Daehyun Kim ◽  
Joonho Moon ◽  
Jaejung Ha ◽  
Doyoon Kim ◽  
Junkoo Yi
Keyword(s):  
Immune Response ◽  
Artificial Insemination ◽  
Bos Taurus ◽  
Foot And Mouth Disease ◽  
Test Group ◽  
Mouth Disease ◽  
Control Group ◽  
Amyloid A ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Vaccination against foot-and-mouth disease is the most common method for preventing the spread of the disease; the negative effects include miscarriage, early embryo death, lower milk production, and decreased growth of fattening cattle. Therefore, in this study, we analyze the side effects of vaccination by determining the acute immune response and ovulation rate after vaccinating cows for foot-and-mouth disease. The test axis was synchronized with ovulation using 100 Hanwoo (Bos taurus coreanae) cows from the Gyeongsangbuk-do Livestock Research Institute; only individuals with estrus confirmed by ovarian ultrasound were used for the test. All test axes were artificially inseminated 21 days after the previous estrus date. The control group was administered 0.9% normal saline, the negative control was injected intramuscularly with lipopolysaccharide (LPS; 0.5 µg/kg), and the test group was administered a foot-and-mouth disease virus vaccine (FMDV vaccine; bioaftogen, O and A serotypes, inactivated vaccine) 2, 9, and 16 days before artificial insemination. White blood cells and neutrophils increased significantly 1 day after vaccination, and body temperature in the rumen increased for 16 h after vaccination. Ovulation was detected 1 day after artificial fertilization by ovarian ultrasound. The ovulation rates were as follows: control 89%, LPS 60%, FMDV vaccine (−2 d) 50%, FMDV vaccine (−9 d) 75%, and FMDV vaccine (−16 d) 75%. In particular, the FMDV vaccine (−2 d) test group confirmed that ovulation was delayed for 4 days after artificial insemination. In addition, it was confirmed that it took 9 days after inoculation for the plasma contents of haptoglobin and serum amyloid A to recover to the normal range as the main acute immune response factors. The conception rate of the FMDV vaccine (−2 d) group was 20%, which was significantly lower than that of the other test groups.

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