scholarly journals Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia

1999 ◽  
Vol 123 (1) ◽  
pp. 149-155 ◽  
Author(s):  
H. C. DAVISON ◽  
M. V. THRUSFIELD ◽  
S. MUHARSINI ◽  
A. HUSEIN ◽  
S. PARTOUTOMO ◽  
...  
Keyword(s):  
Operating Characteristic ◽  
False Negative ◽  
Trypanosoma Evansi ◽  
Antibody Detection ◽  
Test Results ◽  
Likelihood Ratios ◽  
Significant Difference ◽  
Post Test ◽  
Positive Results ◽  
Rule Out

Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanasoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which had been found to be infected by parasitological tests. Diagnostic specificity was estimated by testing sera from 263 buffaloes living in Australia. Response-operating characteristic curves were constructed, and optimal ELISA cut-off values, which minimized the number of false–negative and false–positive results, were chosen. The IgG ELISA had the highest sensitivity (89%) and the CATT had the highest specificity (100%). There was a significant difference between the sensitivities (71 and 81%), but not between the specificities (75 and 78%), of the two Ag-ELISAs. The four tests were further compared by calculation of post-test probabilities of infection for positive and negative test results using a range of prevalence values, and likelihood ratios. The results suggested that the CATT was the best test to ‘rule-in’ infection (i.e. the highest probability of infection in test-positive animals) and the IgG ELISA was the best test to ‘rule-out’ infection (i.e. the lowest probability of infection in test-negative animals).

Three Years of Experience With Random Urinary Homovanillic and Vanillylmandelic Acid Levels in the Diagnosis of Neuroblastoma

PEDIATRICS ◽  
1987 ◽  
Vol 79 (2) ◽  
pp. 203-205
Author(s):  
Mendel Tuchman ◽  
Margaret L. R. Ramnaraine ◽  
William G. Woods ◽  
William Krivit
Keyword(s):  
False Positive ◽  
Homovanillic Acid ◽  
False Negative ◽  
False Negative Rate ◽  
Urine Samples ◽  
Vanillylmandelic Acid ◽  
Test Results ◽  
Upper Limit ◽  
Positive Results ◽  
Rule Out

During the last 3 years, random urine samples from 408 patients were tested for elevated homovanillic acid (HVA) and vanillylmandelic acid (VMA) levels to rule out the diagnosis of neuroblastoma. Thirty-seven of these patients had elevated HVA and/or VMA levels, and neuroblastoma was subsequently diagnosed. In three additional patients with negative test results (normal HVA and VMA levels), tumors were subsequently diagnosed (false-negative rate of 7.5%). Ten percent of the patients with neuroblastoma had normal HVA and 27.5% had normal VMA levels at the time of diagnosis. Only one patient (2.5%) with neuroblastoma had elevated VMA levels in the presence of normal HVA levels. More than 60% of the patients with neuroblastoma had urinary HVA and/or VMA levels higher than twice the upper limit of normal. No false-positive results were encountered. Age and stage distributions of the patients are shown, and the significance of the results is discussed.

scholarly journals Diagnostic parameters of CK–MB and myoglobin related to chest pain duration

CJEM ◽  
2002 ◽  
Vol 4 (05) ◽  
pp. 322-330 ◽  
Author(s):  
Grant Innes ◽  
James Christenson ◽  
W. Douglas Weaver ◽  
Tiepu Liu ◽  
James Hoekstra ◽  
...  
Keyword(s):  
Chest Pain ◽  
Test Results ◽  
Likelihood Ratios ◽  
Cardiac Marker ◽  
Post Test ◽  
Pain Duration ◽  
Maximal Sensitivity ◽  
Ongoing Pain ◽  
Sensitivity Specificity ◽  
Rule Out

ABSTRACT:Objective:Cardiac marker sensitivity depends on chest pain duration at the time of sampling. Our objective was to estimate the sensitivity, specificity, and likelihood ratios of early CK–MB and myoglobin assays in patients presenting to the emergency department (ED) with nondiagnostic ECGs, stratified by the duration of ongoing chest pain at the time of ED assessment.Methods:This was a prospective observational study carried out in 10 US and 2 Canadian EDs. Patients >25 years of age with ongoing chest pain and nondiagnostic ECGs were stratified by pain duration (0–4 h, 4–8 h, 8–12 h, >12 h). CK–MB and myoglobin assays were drawn at T = 0 (ED assessment) and T = 1 hr. Patients were followed for 7–14 days to identify all cases of acute myocardial infarction (AMI). ED test results were correlated with patient outcomes.Results:Of 5005 eligible patients, 565 had AMI. Pain duration was 0–4 h in 3014 patients, 4–8 h in 961, 8–12 h in 487, and >12 h in 543. Marker sensitivity increased with pain duration, ranging from 28%–77% for CK–MB and 39%–73% for myoglobin. The maximal sensitivity achieved by a T = 0 assay was 73%, and this was in patients with 8–12 or >12 h of ongoing pain. No combination of tests achieved 90% sensitivity in any pain duration strata.Conclusions:Regardless of chest pain duration, single assays and early serial markers (0+1 hr) do not rule out AMI; therefore, serial assays over longer observation periods are required. Likelihood ratios derived in this study will help physicians who use Bayesian analysis to determine post-test AMI likelihood in patients with chest pain.

scholarly journals The Use of Keyword Video Captioning on Vocabulary Learning Through Mobile-Assisted Language Learning

2017 ◽  
Vol 7 (4) ◽  
pp. 1 ◽  
Author(s):  
Hassan Saleh Mahdi
Keyword(s):  
Language Learning ◽  
Mobile Devices ◽  
Vocabulary Learning ◽  
Test Results ◽  
Video Captioning ◽  
Mobile Assisted Language Learning ◽  
Significant Difference ◽  
Post Test ◽  
Key Aspects ◽  
Teaching Pronunciation

Video captioning is a useful tool for language learning. In the literature, video captioning has been investigated by many studies and the results indicated that video captioning may foster vocabulary learning. Most of the previous studies have investigated the effect of full captions on vocabulary learning. One of the key aspects of vocabulary learning is pronunciation. However, the use of mobile devices for teaching pronunciation has not been investigated conclusively. Therefore, this paper attempts to examine the effect of implementing keyword video captioning on L2 pronunciation using mobile devices. Thirty-four Arab EFL university learners participated in this study and were randomly assigned to two groups (key-word captioned video and full captioned video). The study is an experimental one in which pre- and post-tests were administered to both groups. The results indicated that keyword captioning is a useful mode to improve learner’s pronunciation. The post test results indicate that there was no statistically significant difference between the two modes of captioning on vocabulary learning. However, learners at keyword video captioning performed better that full video captioning. 

scholarly journals The Effect Of Counseling On The Adherence Of Therapeutic Hypertension Patients

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Gita Sekar Prihanti ◽  
Novi Puspita Sari ◽  
Nur Indah Septiani ◽  
Laura Putri Risty L. Tobing ◽  
Annisa Rahayu Adrian ◽  
...  
Keyword(s):  
Family Support ◽  
Statistical Tests ◽  
Adherence Rate ◽  
Test Results ◽  
A Value ◽  
Significant Difference ◽  
Post Test ◽  
Lifestyle Education ◽  
Nonparametric Statistical ◽  
Adherence To Therapy

Failure of therapy is a result of bad adherence  medication. Non-adherence to therapy is a major factor that is suspected to result in uncontrolled blood pressure in hypertensive patients resulting in more serious complications. Therefore it is important to increase the adherence rate of treatment in patients with hypertension in the treatment process. For this reason, the need for interventions to improve  adherence  with several aspects that can be changed. This study uses One Group Pretest-Posttest Design using 100 samples. Data derived from questionnaires containing 25 items of adherence to therapy, 5 items of knowledge, 4 items of trust, 3 items of motivation, 10 items of family support with nonparametric statistical tests used were Mc Nemar test. Mc Nemar test results indicate that there is a significant difference between adherence at the pre-test and at the post-test after counseling with a significance value (p = 0,000). The results also showed that there was a difference in knowledge with a significance value (p = 0.001), motivation with a value (p = 0.031) and family support with a value (p = 0,000). The education with counseling about knowledge, trust, motivation and family support is effective in increasing compliance. There are other changeable factors such as lifestyle education, patient doctor relationships, and the use of smartphone applications for self-reported therapy can improve adherence in patients thereby minimizing therapy failure. Other educational methods that can be used besides counseling are counseling and dissemination of social media information.

scholarly journals PENGARUH PSIKOEDUKASI TERHADAP PENGETAHUAN INKLUSIVITAS PADA ANAK-ANAK DI YAYASAN PANTI ASUHAN Z

2021 ◽  
pp. 1429
Author(s):  
Agnes Nugrawati Salim ◽  
Atalya Debora ◽  
Farica Tanojo ◽  
Sriana Sihombing ◽  
Sylvia Dewi Suryaganda ◽  
...  
Keyword(s):  
Social Interaction ◽  
Experimental Design ◽  
Statistical Test ◽  
Test Results ◽  
Significant Difference ◽  
Post Test ◽  
Parametric Statistical Test ◽  
Age Range ◽  
Non Parametric

The research explored the effect of psychoeducation towards knowledge of inclusivity for children at Yayasan Panti Asuhan Z (Z orphanage). Yayasan Panti Asuhan Z (Z orphanage) has been chosen because there is a phenomenon where the typical children shows stigmatization towards the atypical children. There are various form of stigmatization, including physical, attitude, communication, social interaction, et cetera. This research involved 33 children at the Yayasan Panti Asuhan Z having an age range from 11-21 years old. The design used in this research is within-group experimental design. The result of this study indicates that there is an increase after being given psychoeducation. Researchers used the Mann-Whitney non parametric statistical test. The result has shown there is a significant difference between pre-test and post-test results (p < 0.05, N=28).Penelitian ini melihat pengaruh psikoedukasi terhadap pengetahuan inklusivitas pada anak-anak di Yayasan Panti Asuhan Z. Yayasan Panti Asuhan Z dipilih karena adanya fenomena stigmatisasi yang tidak disadari antara anak-anak tipikal terhadap anak-anak atipikal yang tinggal di panti tersebut. Bentuk stigmatisasi yang terjadi beragam, seperti fisik, sikap, cara berkomunikasi, cara berteman, dan sebagainya. Penelitian ini melibatkan 33 anak-anak di Yayasan Panti Asuhan Z yang berusia mulai dari 11 hingga 21 tahun. Desain yang digunakan pada penelitian ini adalah within-group experimental design. Hasil dari penelitian ini menunjukkan bahwa terdapat peningkatan pengetahuan inklusivitas yang ditandai dengan meningkatnya hasil pre-test setelah diberikan psikoedukasi. Peneliti menggunakan uji statistik non-parametrik Mann Whitney. Berdasarkan hasil uji beda tersebut, diketahui bahwa hasil pre-test dan post-test memiliki perbedaan yang signifikan (p < 0.05, N=28).

Improved diagnosis of viable parenchymal neurocysticercosis by combining antibody banding patterns on enzyme-linked immunoelectrotransfer blot (EITB) with antigen ELISA assay

2021 ◽  
Author(s):  
Gianfranco Arroyo ◽  
Javier A. Bustos ◽  
Andres G. Lescano ◽  
Isidro Gonzales ◽  
Herbert Saavedra ◽  
...  
Keyword(s):  
Latent Class ◽  
False Negative ◽  
Antibody Detection ◽  
Elisa Assay ◽  
True Positive ◽  
Banding Patterns ◽  
Exact Test ◽  
Antigen Elisa ◽  
Class 1 ◽  
Rule Out

The diagnosis of NCC depends on neuroimaging and serological confirmation. While antibody detection by enzyme-linked immunoelectrotransfer blot (EITB) fails to predict viable NCC, EITB banding patterns provide information about the host’s infection course. Adding antigen ELISA results on EITB banding patterns may improve their ability to predict or rule out of viable NCC. We assessed whether combining EITB banding patterns with Ag-ELISA improves discrimination of viable infection in imaging-confirmed parenchymal NCC. EITB banding patterns were grouped into classes using latent class analysis. True-positive and false-negative Ag-ELISA results in each class were compared using Fisher’s exact test. Four classes were identified: 1 (EITB-negative or positive to GP50 alone [GP50 antigen family]), 2 (positive to GP42-39 and GP24 [T24/42 family], with or without GP50), 3 and 4 (positive to GP50, GP42-39 and GP24, and reacting to bands in the 8-kDa family). Most cases in classes 3 and 4 had viable NCC (82% and 88%) compared to classes 2 and 1 (53% and 5%). Adding positive Ag-ELISA results to class 2 predicted all viable NCC cases (22/22 [100%]), whereas 11/40 patients (27.5%) Ag-ELISA negative had viable NCC ( P < 0.001). Only 1/4 patients (25%) Ag-ELISA positive in class 1 had viable NCC, whereas 1/36 patients (2.8%) Ag-ELISA negative had viable NCC ( P = 0.192). In classes 3 and 4, adding Ag-ELISA was not contributory. Combining Ag-ELISA with EITB banding patterns improves discrimination of viable from non-viable NCC, particularly for class-2 responses. Together, these complement neuroimaging more appropriately for the diagnosis of viable NCC.

Spiral computed tomography for the diagnosis of acute pulmonary embolism

2007 ◽  
Vol 98 (10) ◽  
pp. 713-720 ◽  
Author(s):  
Fadi Kayali ◽  
Russell Hull ◽  
Paul Stein
Keyword(s):  
Computed Tomography ◽  
Pulmonary Embolism ◽  
Acute Pulmonary Embolism ◽  
Multidetector Ct ◽  
False Negative ◽  
Positive Likelihood Ratio ◽  
Multislice Ct ◽  
Likelihood Ratios ◽  
Post Test ◽  
Single Detector

SummaryThe accuracy of computed tomography (CT) imaging for the diagnosis of acute pulmonary embolism (PE) was reviewed. Single detector CT, based on pooled data, showed a sensitivity of 73% and multidetector CT, mostly 4-slice, showed a sensitivity of 83%. Respective specificities were 87% and 96%. Among patients with suspected PE evaluated with single slice CT,20% of patients found to have venous thromboembolic disease were diagnosed on the basis of a positive CT venous phase venogram. With multislice CT, 14% were diagnosed on the basis of a positive CT venogram.The positive likelihood ratio with single detector CT was 5.7 and with multidetector CT it was 19.6. Respective negative likelihood ratios were 0.31 and 0.18. Calculations of post-test probability using pretest probability and likelihood ratios according to Bayes’ theorem showed that even with multidetector CT, false positive and false negative images are not uncommon when clinical assessment is discordant with the CT interpretation. Outcome studies showed recurrent PE in only 1.7% or fewer untreated patients with negative CT pulmonary angiograms.

scholarly journals The Basic Motion Learning Model of Small Game Based Throwing in Elementary School

2021 ◽  
Vol 5 (2) ◽  
pp. 95
Author(s):  
Gunawan Setiawan ◽  
Syamsuramel Syamsuramel ◽  
Silvi Aryanti
Keyword(s):  
Elementary School ◽  
Learning Model ◽  
Small Scale ◽  
Test Results ◽  
Small Game ◽  
Motion Learning ◽  
Significant Difference ◽  
Post Test ◽  
The Difference ◽  
Basic Motion

This study aims to develop a small game-based basic motion learning model at Elementary School Number 65 Rejang Lebong. The research method used is research and development (R&D) using ten stages from Borg and Gall. The research instruments used were locomotor, non-locomotor tests, and manipulative basic movement skills tests. The treatment in this study was in the form of a basic motion learning model based on small games given for 4 weeks. The subjects in this study were students in grades 4 and 5 of Elementary School Number 65 Rejang Lebong. Subjects in this study were 30 people. The validation results obtained in small-scale trials regarding the quality of the learning model have entered into the validation criteria filled by the validator with a percentage of 63% and the results of large group trials are declared valid with a percentage of 81.25% which means very feasible. Furthermore, the effectiveness test was carried out using the t-test. In the significance test of the difference using the SPSS tool, the mean value = 0.8666 which shows the difference between the pre-test and post-test results, the results of T-count = 13.730, df = 29, and P-Value = 0.000 <0.05, which means that there are There is a significant difference between before and after being treated with the small game-based learning model of throwing basic motion learning. Where the post-test results have positive results with a significant difference compared to the pre-test results. So that the learning model of the basic motion of throwing based on small games can be used in learning physical education and health in elementary schools. The implication of this research is that the basic motion learning model of throwing based on small games can be used as a form of learning the basic motion of throwing in elementary school children.

scholarly journals PENERAPAN METODEBERMAIN ANAK USIA DINI PADA KELOMPOK B UNTUK MENINGKATKAN KEMAMPUAN BERHITUNG PERMULAAN DI TK AL-FALAH

2019 ◽  
Vol 2 (3) ◽  
pp. 84
Author(s):  
Rina Septiadiningsih ◽  
Euis Rohaety ◽  
Lenny Nuraeni
Keyword(s):  
Design Research ◽  
Sensitive Period ◽  
Control Group ◽  
P Value ◽  
Test Results ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
The Right ◽  
In The Beginning

Sensitive period is the right time to instill the basis of the importance of counting. In addition to counting skills that must be developed also pay attention to the application of learning used. However, in the learning process, teachers often forget the importance of playing methods. If too often with giving assignments the child will feel bored, therefore what is used is the method of playing. Play method is one method that can be used by teachers to teach the ability to count children in the beginning. Based on this background, the purpose of this study was to determine the effect of the use of playing methods on initial numeracy skills. This study used the Quasi Experiment method with the Nonequevalent Control Group Design research design. The results of the pre test showed that there were no significant differences in the experimental group and the control group with p-value> 0.05, which was 0.135> 0.05 which was tested by the two average test results. While the post-test results showed that the initial numeracy ability after the application of the playing method experienced a significant difference with the p-value <0.05, that is with the results of 0.0001 with the Mann-Whitney test. This method of playing can be used as a learning suggestion for educators to improve their ability to have an early start.

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