scholarly journals Lipocalin 2 as a potential systemic biomarker for central serous chorioretinopathy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
A. Matet ◽  
T. Jaworski ◽  
E. Bousquet ◽  
J. Canonica ◽  
C. Gobeaux ◽  
...  
Keyword(s):  
Central Serous Chorioretinopathy ◽  
Recurrent Disease ◽  
Serum Levels ◽  
Immune Dysregulation ◽  
Innate Immune ◽  
Healthy Controls ◽  
Lipocalin 2 ◽  
Cutoff Value ◽  
Retinal Disorders ◽  
Age And Sex

AbstractNo systemic biomarker of Central Serous Chorioretinopathy (CSCR) has been identified. Lipocalin 2 (LCN2 or NGAL), alone or complexed with MMP-9 (NGAL/MMP-9), is increased in several retinal disorders. Serum levels of LCN2 and NGAL/MMP-9 were measured in CSCR patients (n = 147) with chronic (n = 76) or acute/recurrent disease (n = 71) and in age- and sex-matched healthy controls (n = 130). Samples with CRP > 5 mg/L, creatinine > 100 µmol/L, and/or urea > 7.5 mmol/L were excluded. Serum LCN2 was lower in CSCR patients than controls (81.4 ± 48.7 vs 107.3 ± 44.5 ng/ml, p < 0.0001), and lower in acute/recurrent CSCR than controls (p < 0.001) and chronic CSCR (p = 0.006). Serum NGAL/MMP-9 was lower in CSCR patients than controls (47.2 ± 40.7 vs 74.1 ± 42.6, p < 0.0001), and lower in acute/recurrent CSCR than controls (p < 0.001) and chronic CSCR (p = 0.002). A ROC curve showed that for LCN2 serum levels, the 80-ng/ml cutoff value allows to discriminate acute/recurrent CSCR from controls with 80.3% sensitivity and 75.8% specificity, and for NGAL/MMP-9 serum levels, a 38-ng/ml cutoff value allows to discriminate acute/recurrent CSCR from controls with 69.6% sensitivity and 80.3% specificity. In both acute and chronic CSCR, low serum LCN2 and NGAL/MMP-9, provide a biological link between the two CSCR forms, and potential susceptibility to oxidative stress and innate immune dysregulation in CSCR.

scholarly journals Combination of serum and CSF neurofilament-light and neuroinflammatory biomarkers to evaluate ALS

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexandre Brodovitch ◽  
José Boucraut ◽  
Emilien Delmont ◽  
Amandine Parlanti ◽  
Aude-Marie Grapperon ◽  
...  
Keyword(s):  
Serum Levels ◽  
Healthy Controls ◽  
Peripheral Neuropathies ◽  
Neurofilament Light ◽  
Cutoff Value ◽  
Csf Analysis ◽  
Diagnostic Potential ◽  
Ifn Γ ◽  
Als Patients ◽  
Lateral Sclerosis

AbstractThis monocentric prospective study of patient suffering from Amyotrophic lateral sclerosis (ALS) aims to evaluate the prognosis and diagnostic potential of both Neurofilament-Light (Nf-L) and neuroinflammatory biomarkers in serum and CSF. Candidate markers levels were measured using multiplex method in serum of 60 ALS patients, 94 healthy controls of 43 patients suffering from Inflammatory Peripheral Neuropathies (IPN). A comparative CSF analysis was performed for 20 ALS and 17 IPN patients. Among the altered biomarkers, CSF Nf-L level remains the best marker of ALS severity, while serum levels correlate strongly with disease progression. The combination of Nf-L and ICAM-1 concentrations in the CSF and IFN-γ concentration in the serum differentiate ALS patients from IPN patients with improved sensibility and specificity relative to individual biomarkers. A cutoff value of 0.49 for the fitted values of these 3 biomarkers discriminate ALS from IPN patients with a specificity of 100% (78.20–100%) and a sensibility of 85.71% (57.19–98.22%) with an AUC of 0.99 ± 0.01. The measure of Nf-L and neuroinflammatory biomarkers in CSF and serum can be useful biomarkers panel in the differential diagnosis of ALS.

Homozysteinspiegel von Patienten mit Raynaud-Phänomen

VASA ◽  
2002 ◽  
Vol 31 (2) ◽  
pp. 87-90 ◽  
Author(s):  
Al-Awami ◽  
Schillinger ◽  
Maca ◽  
Gschwandtner ◽  
Bieglmayer ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Plasma Levels ◽  
Serum Levels ◽  
Plasma Homocysteine ◽  
Serum Folate ◽  
Vitamin B ◽  
Healthy Controls ◽  
Healthy Individuals ◽  
Homocysteine Concentration ◽  
Age And Sex

Background: Patients with Raynaud’s phenomenon (RP) have vasomotor dysregulation, mainly caused by dysfunction of the endothelium. Since homocysteine has been found to be damaging to endothelial cells, we investigated the concentrations of plasma homocysteine, folate and vitamin B12 in patients with primary or secondary RP compared to healthy individuals. Patients and methods: We measured the concentrations of plasma fasting homocysteine, folate and vitamin B12 in a group of healthy individuals (n = 45) and in patients with primary (n = 26) or secondary RP (n = 42). Results: Median homocysteine levels in healthy controls and in patients with primary RP, secondary RP were 7.9 (IQR 4.1 to 11.8) 9.8 (IQR 5.1 to14.4), and 10.6 (6.0 to15.3) mumol/L, respectively. Patients with primary and secondary RP had significantly higher homocysteine concentration compared to healthy controls (Kruskal Wallis p = 0.01). After matching for age and sex, patients with either primary or secondary RP showed significantly higher homocysteine levels (Wilcoxon p < 0.0001). No significant differences between the three groups were found concerning serum levels of vitamin B12 (p = 0.9 ) and serum folate levels (p = 0.2). Conclusion: These data demonstrate that patients with RP have higher plasma levels of homocysteine. No significant differences in folate and vitamin B12 levels were found between patients with primary RP, secondary RP, and healthy individuals.These data suggest that homocysteine may play a role in RP and may provide new clues in understanding of the vasomotor dysregulation.

scholarly journals Serum Total Protein, Albumin, Globulin, and Prealbumin in Acne Patients

2020 ◽  
Author(s):  
Hao Jiang ◽  
Changyi Li ◽  
bin wei ◽  
Qiang Wang ◽  
Qinggao Wang ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Total Protein ◽  
Serum Levels ◽  
Control Group ◽  
Healthy Controls ◽  
Serum Globulin ◽  
Serum Total Protein ◽  
Protein Levels ◽  
Healthy Control ◽  
Age And Sex

Abstract Background/Objectives: This study was designed to investigate serum protein levels in acne patients.Method: Acne patients (n=362) and healthy volunteers (n=272) were matched in terms of both age and sex. Serum levels were measured.Results: Among the 362 acne patients and 272 age- and sex-matched healthy controls, serum albumin levels in female acne patients were lower than in the healthy controls (P < 0.05), serum albumin levels in male acne patients were lower than in the healthy controls (P < 0.01). Additionally, serum globulin and total protein levels were significantly lower in acne patients than in the healthy control group (P < 0.01). Serum levels of prealbumin were significantly lower in female acne patients than in the control group (P < 0.05). Finally, the severity of female and male acne patients was negatively correlated with serum total protein, albumin, globulin, and prealbumin levels. Conclusions: The results of this study suggested that acne patients are potentially accompanied with protein malnutrition.

The Influence of Valproic Acid and Carbamazepine Treatment on Serum Biotin and Zinc Levels and on Biotinidase Activity

2011 ◽  
Vol 26 (12) ◽  
pp. 1522-1524 ◽  
Author(s):  
Manuel Castro-Gago ◽  
Laura Pérez-Gay ◽  
Carmen Gómez-Lado ◽  
Daisy E. Castiñeiras-Ramos ◽  
Santiago Otero-Martínez ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Enzyme Activity ◽  
Serum Levels ◽  
Normal Serum ◽  
Control Group ◽  
Healthy Controls ◽  
Normal Range ◽  
Zinc Levels ◽  
Biotinidase Activity ◽  
Age And Sex

We determined the serum concentration of biotin, zinc, antiepileptic drugs, and biotinidase enzyme activity in 20 children treated with valproic acid, in 10 children treated with carbamazepine, and in 75 age- and sex-matched healthy controls. There were no significant differences in the serum levels of biotin, and biotinidase enzyme activity between the patients treated with valproic acid, the patients treated with carbamazepine, and the control group. Zinc serum levels were lower in the patients treated with valproic acid and with carbamazepine than in the control group, but within the normal range. Hair loss was observed in 3 patients treated with valproic acid, with normal serum levels of biotin, zinc, and biotinidase activity, and the alopecia disappeared with the oral administration of biotin (10 mg/d) in 3 months. These results suggest that the treatment with valproic acid does not alter the serum levels of biotin, zinc, and biotinidase enzyme activity.

scholarly journals Osteoprotegerin serum levels in multiple myeloma and MGUS patients compared with age- and sex-matched healthy controls

Leukemia ◽  
2004 ◽  
Vol 18 (9) ◽  
pp. 1555-1557 ◽  
Author(s):  
A Corso ◽  
A Dovio ◽  
C Rusconi ◽  
M L Sartori ◽  
C Klersy ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Serum Levels ◽  
Healthy Controls ◽  
Age And Sex

scholarly journals Evaluation of the Human Pulmonary Activation-Regulated Chemokine (CCL18/PARC) and Alkaline Phosphatase (ALP) Levels in Iraqi Patients with Rheumatoid Arthritis

2020 ◽  
pp. 713-719
Author(s):  
Nawal Haider Al-Hashimi ◽  
Abdulnasser M. Al-Gebori ◽  
Mohammed Hadi Munshed Alosami
Keyword(s):  
Rheumatoid Arthritis ◽  
Alkaline Phosphatase ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Blood Samples ◽  
Fourth Group ◽  
The Third ◽  
Erythrocyte Sedimentation ◽  
Age And Sex

Rheumatoid arthritis (RA) is one of the chronic inflammatory autoimmune diseases which occurs as a result of unknown reasons. This study was conducted at Baghdad Teaching Hospital/City of Medicine, where blood samples were taken from 60 Iraqi patients with RA (49 females and 11 males) and these patients were matched by age and sex with 20 healthy controls (16 females and 4 males). Patients with RA were diagnosed by a consultant rheumatologist according to ACR / EULAR criteria in 2010. In this study the patients were divided into four groups as follows; the first group consisted of 12 patients treated with methotrexate (MTX), the second group consisted of 10 patients treated with etanercept, the third group consisted of 18 patients treated with a combination of MTX, etanercept and prednisolone, the fourth group consisted of 20 patients treated with MTX and etanercept. Enzyme linked immunosorbent assay (ELISA) was used to detect CCL18/PARC antibodies, while a spectrophotometer (Humalyzer2000) was used for the measurement of alkaline phosphatase (ALP). Serum levels of CCL18 / PARC showed a significant increase in RA patients compared with healthy controls (p ≤ 0.001). The levels of CCL18/PARC showed a significant correlation with disease activity (CDAI), except in RA patients treated with etanercept. There was also no significant correlation between CCL18/PARC and erythrocyte sedimentation rate (ESR). The results showed a significant increase in serum levels of alkaline phosphatase (ALP) was recorded in RA patients (with treatment) as compared to healthy controls (p ≤ 0.001).

Characterizing Possible Biomarkers in Ascending Aortic Aneurysms - MMP-2/9 Serum Levels in Patients and Healthy Controls

2015 ◽  
Vol 63 (S 01) ◽  
Author(s):  
P. Meffert ◽  
A. Tscheuschler ◽  
N.-M. Kocher ◽  
X. Uffelmann ◽  
M. Russe ◽  
...  
Keyword(s):  
Serum Levels ◽  
Aortic Aneurysms ◽  
Healthy Controls

scholarly journals Lipocalin 2 serum levels correlate with age and bone turnover biomarkers in healthy subjects but not in postmenopausal osteoporotic women

Bone Reports ◽  
2021 ◽  
pp. 101059
Author(s):  
Antonio Maurizi ◽  
Marco Ponzetti ◽  
Kaare M. Gautvik ◽  
Sjur Reppe ◽  
Anna Teti ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Healthy Subjects ◽  
Serum Levels ◽  
Lipocalin 2 ◽  
Bone Turnover Biomarkers

scholarly journals Folate and Cobalamin Serum Levels in Healthy Children and Adolescents and Their Association with Age, Sex, BMI and Socioeconomic Status

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 546
Author(s):  
Paulina Kreusler ◽  
Mandy Vogel ◽  
Anja Willenberg ◽  
Ronny Baber ◽  
Yvonne Dietz ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Children And Adolescents ◽  
Serum Levels ◽  
Population Based ◽  
Research Centre ◽  
Healthy Children ◽  
Serum Samples ◽  
Lms Method ◽  
Healthy Participants ◽  
Age And Sex

This study proposes age- and sex-specific percentiles for serum cobalamin and folate, and analyzes the effects of sex, age, body mass index (BMI), and socioeconomic status (SES) on cobalamin and folate concentrations in healthy children and adolescents. In total, 4478 serum samples provided by healthy participants (2 months–18.0 years) in the LIFE (Leipzig Research Centre for Civilization Diseases) Child population-based cohort study between 2011 and 2015 were analyzed by electrochemiluminescence immunoassay (ECLIA). Continuous age-and sex-related percentiles (2.5th, 10th, 50th, 90th, 97.5th) were estimated, applying Cole’s LMS method. In both sexes, folate concentrations decreased continuously with age, whereas cobalamin concentration peaked between three and seven years of age and declined thereafter. Female sex was associated with higher concentrations of both vitamins in 13- to 18-year-olds and with higher folate levels in one- to five-year-olds. BMI was inversely correlated with concentrations of both vitamins, whilst SES positively affected folate but not cobalamin concentrations. To conclude, in the assessment of cobalamin and folate status, the age- and sex-dependent dynamic of the respective serum concentrations must be considered. While BMI is a determinant of both vitamin concentrations, SES is only associated with folate concentrations.

Serum Concentration and Skin Expression of S100A7 (Psoriasin) in Patients Suffering from Hidradenitis Suppurativa

Dermatology ◽  
2020 ◽  
pp. 1-7
Author(s):  
Aleksandra Batycka-Baran ◽  
Wojciech Baran ◽  
Danuta Nowicka-Suszko ◽  
Maria Koziol-Gałczyńska ◽  
Andrzej Bieniek ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Hidradenitis Suppurativa ◽  
Protein Concentration ◽  
Normal Skin ◽  
Innate Immune ◽  
Antimicrobial Protein ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Reactive Protein

<b><i>Background:</i></b> Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease. An important role of innate immune dysregulation in the pathogenesis of HS has been highlighted. S100A7 (psoriasin) is an innate, antimicrobial protein that exerts proinflammatory and chemotactic action. <b><i>Objectives:</i></b> The objective of the study was to investigate serum concentrations of S100A7 in individuals with HS as compared to healthy controls. Further, we evaluated the expression of S100A7 in lesional HS skin as compared to perilesional (clinically uninvolved) HS skin and normal skin. <b><i>Methods:</i></b> Serum concentrations of S100A7 were evaluated with a commercially available ELISA kit. The expression of S100A7 in the skin was assessed using qRT-PCR and immunofluorescence staining. <b><i>Results:</i></b> We found increased expression of S100A7 in lesional HS skin as compared to perilesional HS skin (<i>p</i> = 0.0017). The expression of S100A7 in lesional HS skin was positively associated with serum C-reactive protein concentration and the severity of disease according to Hurley staging. The serum concentration of S100A7 in individuals with HS was decreased as compared to healthy controls and patients with psoriasis. <b><i>Conclusions:</i></b> Upregulated in lesional HS skin, S100A7 may enhance the inflammatory process and contribute to the HS pathogenesis.

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