The Influence of Valproic Acid and Carbamazepine Treatment on Serum Biotin and Zinc Levels and on Biotinidase Activity

We determined the serum concentration of biotin, zinc, antiepileptic drugs, and biotinidase enzyme activity in 20 children treated with valproic acid, in 10 children treated with carbamazepine, and in 75 age- and sex-matched healthy controls. There were no significant differences in the serum levels of biotin, and biotinidase enzyme activity between the patients treated with valproic acid, the patients treated with carbamazepine, and the control group. Zinc serum levels were lower in the patients treated with valproic acid and with carbamazepine than in the control group, but within the normal range. Hair loss was observed in 3 patients treated with valproic acid, with normal serum levels of biotin, zinc, and biotinidase activity, and the alopecia disappeared with the oral administration of biotin (10 mg/d) in 3 months. These results suggest that the treatment with valproic acid does not alter the serum levels of biotin, zinc, and biotinidase enzyme activity.

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Serum Total Protein, Albumin, Globulin, and Prealbumin in Acne Patients

10.21203/rs.3.rs-122981/v1 ◽

2020 ◽

Author(s):

Hao Jiang ◽

Changyi Li ◽

bin wei ◽

Qiang Wang ◽

Qinggao Wang ◽

...

Keyword(s):

Serum Albumin ◽

Total Protein ◽

Serum Levels ◽

Control Group ◽

Healthy Controls ◽

Serum Globulin ◽

Serum Total Protein ◽

Protein Levels ◽

Healthy Control ◽

Age And Sex

Abstract Background/Objectives: This study was designed to investigate serum protein levels in acne patients.Method: Acne patients (n=362) and healthy volunteers (n=272) were matched in terms of both age and sex. Serum levels were measured.Results: Among the 362 acne patients and 272 age- and sex-matched healthy controls, serum albumin levels in female acne patients were lower than in the healthy controls (P < 0.05), serum albumin levels in male acne patients were lower than in the healthy controls (P < 0.01). Additionally, serum globulin and total protein levels were significantly lower in acne patients than in the healthy control group (P < 0.01). Serum levels of prealbumin were significantly lower in female acne patients than in the control group (P < 0.05). Finally, the severity of female and male acne patients was negatively correlated with serum total protein, albumin, globulin, and prealbumin levels. Conclusions: The results of this study suggested that acne patients are potentially accompanied with protein malnutrition.

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Oxidative stress in children with severe acute malnutrition between 6 months to 5 years of age

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20182434 ◽

2018 ◽

Vol 5 (4) ◽

pp. 1327 ◽

Cited By ~ 2

Author(s):

Komal Shrivastava ◽

Ajay Gaur ◽

Ravi Ambey

Keyword(s):

Oxidative Stress ◽

Severe Acute Malnutrition ◽

Management Strategies ◽

Serum Levels ◽

Cellular Level ◽

Acute Malnutrition ◽

Control Group ◽

Healthy Controls ◽

Mortality And Morbidity ◽

Zinc Levels

Background: Severe acute malnutrition (SAM) constitutes one of the major nutritional and health problems in children under five years of age in developing countries. It has a significant contribution to the mortality and morbidity in this age group of children. So, there is a need to look for the pathophysiology identifying events at cellular level to formulate better management strategies. The oxidative stress and a possible consequential accelerated apoptosis may contribute to pathophysiology in malnutrition. Markers of oxidative stress are Malondialdehyde levels (MDA), a byproduct of lipid peroxidation and antioxidants like Zinc and Glutathione (GSH). So the objective of study is to measure oxidative stress in hospitalized children with severe acute malnutrition (SAM) between 6 months to 5 years of age through serum levels of Zinc, glutathione and MDA.Methods: This was a hospital based prospective case control study where 100 hospitalised children from 6 months to 5 years of age with severe acute malnutrition were selected along with 100, sex and age matched healthy controls from outpatient department. Oxidative Stress was analysed by measuring serum levels of Zinc, GSH and MDA. Control group was simultaneously analysed for similar parameters of oxidative stress.Results: Serum GSH and Zinc levels were found to be significantly lower while serum MDA level was found to be significantly higher in cases as compared to controls.Conclusions: Severe acute malnourished children had an increased oxidative stress and decreased antioxidant defence compared with healthy controls.

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Exploring The Clinical Significance of Serum Angiopoietin-2 In Breast Cancer Patients

QJM ◽

10.1093/qjmed/hcab091.014 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Abeer I Abd Elmagid ◽

Hala Abdel Al ◽

Wessam El Sayed Saad ◽

Seham Kamal Mohamed

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Serum Levels ◽

Tnm Staging ◽

Control Group ◽

Healthy Controls ◽

Breast Cancer Patients ◽

Female Patients ◽

Significant Difference ◽

Angiopoietin 2

Abstract Background Breast cancer is the most common cancer among women and one of the most important causes of death among them.Angiogenesis is an important step for primary tumor growth, invasiveness, and metastases. Angiopoietins are well-recognized endothelial growth factors that are involved in angiogenesis associated with tumors. Aim To explore the diagnostic significance of serum angiopoietin-2 (Ang-2) in breast cancer and to evaluate its prognostic efficacy through studying the degree of its association with the TNM staging of the disease. Patients and Methods This study was conducted on (35) Egyptian female patients who were diagnosed as breast cancer according to histopathological examination of breast biopsy (Group 1, Breast Cancer Patients) and (25) female patients with benign breast diseases (Group II, Pathological Control Patients), in addition to (20) age - matched apparently healthy, free mammogram, females serving as healthy controls (Group III, Healthy Controls). For all participants, measurement of serum Ang-2 was done using enzyme linked immunosorbent assay (ELISA) technique. Results A highly significant increased levels of Ang-2 was observed in breast cancer patients when compared to healthy control group (Z = 4.95, p < 0.01). However, no significant difference was observed in Ang-2 levels between breast cancer patients group and pathological control group (Z = 3.37, p > 0.05). No significant difference was detected in Ang-2 levels in relation to TNM stage and histological grade. No significant correlation was found between Ang-2 levels and serum levels of CA15-3, hormone receptors, HER2/new receptor status (p > 0.05, respectively). Conclusion This study revealed that Ang-2 serum levels were significantly increased in patient with breast cancer compared with healthy controls, indicating that high Ang-2 level is a promising non invasive biomarker for breast cancer diagnosis. However, no significant difference of Ang-2 levels was detected in relation of breast TNM staging in the population studied.

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Shortened platelet half-life in multiple myeloma

Blood ◽

10.1182/blood.v68.2.514.bloodjournal682514 ◽

1986 ◽

Vol 68 (2) ◽

pp. 514-520

Author(s):

E Fritz ◽

H Ludwig ◽

W Scheithauer ◽

H Sinzinger

Keyword(s):

Multiple Myeloma ◽

Half Life ◽

Serum Levels ◽

Statistical Correlation ◽

Control Group ◽

Healthy Controls ◽

Platelet Factor ◽

Healthy Control

Various defects in platelet function have been reported as being associated with multiple myeloma. In 30 myeloma patients and 15 healthy controls, we investigated platelet survival using in vitro labeling of autologous platelets with 111indium-oxine and measuring the in vivo kinetics of the radioisotope. Significantly shortened platelet half- life in patients averaged 73 hours, while platelet half-life in the healthy controls averaged 107 hours. In myeloma patients, serum levels of thromboxane B2, beta-thromboglobulin, and platelet factor 4 were significantly elevated; aggregation indices were within the pathological range; platelet counts and spleen-liver indices, however, were comparable to those of the healthy control group. No statistical correlation was found between platelet half-life and paraprotein concentrations. Our findings suggest an initial--so far unexplained-- intravascular process of platelet activation and consumption that finally manifests in shortened platelet half-life. It seems that overt thrombocytopenia develops only when the compensatory capacity of the bone marrow finally becomes exhausted. Further studies should be able to elucidate the pathophysiologic processes involved.

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Serum Free Light Chain (FLC) Measurement in Multiple Myeloma (MM) Patients (pts) Correlate with Known Monoclonal Paraprotein, Disease Stage and Therapy Response.

Blood ◽

10.1182/blood.v104.11.4907.4907 ◽

2004 ◽

Vol 104 (11) ◽

pp. 4907-4907 ◽

Cited By ~ 1

Author(s):

Monika Engelhardt ◽

Daniel Räpple ◽

Andreas Weis ◽

Emanuel Bisse ◽

Gabriele Ihorst

Keyword(s):

Early Detection ◽

Therapy Response ◽

Clinical History ◽

Serum Levels ◽

Disease Stage ◽

Control Group ◽

High Dose ◽

Normal Range ◽

Monitoring Therapy ◽

The Impact

Abstract So far, data based on small patient (pt) population suggest that the measurement of serum FLC from MM pts undergoing high-dose chemotherapy (HDCT) with stem cell transplantation (SCT) may be a sensitive marker for monitoring therapy success and for early detection of relapse. For further evaluation of the impact of FLCs on the assessment of treatment efficacy of standard- (ST) and HDCT with SCT, we performed a prospective analysis on serial serum specimens from 86 MM and 9 control pts. Measurement of FLC concentration was performed with the commercially available Freelite™ kit (Binding Site). For statistical analysis, pts’ clinical history, age at diagnosis, sex, current state of disease, karyotype and serum parameters, such as ß2-microglobulin, calcium levels and serum creatinine were evaluated. In the control group (NHL=6, AML=1, non-hematological disease=2), median concentrations of kappa(k)- and lambda(l)- FLC were 9.8 mg/l and 12.8 mg/l, respectively, corresponding to reference intervals for healthy individuals with normal kappa(k)/lambda(l)-ratios. In MM, 40 (46.5%) pts displayed kappa(k)-FLC levels above the upper range of 19mg/l, 26 (30%) had lambda(l)-FLC levels above the upper range of 26 mg/l and 9 pts (10.4%) had both elevated kappa(k)- and lambda(l)-FLC serum levels. An abnormal kappa(k)/lambda(l)-ratio was observed in 45 (52,3%) MM pts. Pts with a known kappa(k)-paraprotein (n=58) had a median FLC kappa(k)-concentration of 38 mg/l, but lambda(l)-FLC within the normal range. For pts with a known lambda(l)-paraprotein (n=27), reciprocal findings (76.4 mg/l for lambda(l)- vs kappa(k)-FLC in the normal range) were observed. Pts with responsive disease (CR, PR and SD) had both kappa(k)- and lambda(l)- FLC levels within the normal range, whereas newly diagnosed pts (ED) and those with PD had kappa(k)- FLC levels approx. 3-times the normal range, with lambda(l)- FLC levels at the upper limit of normal. Pts receiving ST as compared with HDCT had higher FLC levels. This is also observed in pts with amyloidosis, renal impairment or PD. Our results suggest that serum FLC assay allows monitoring of the therapy response and early detection of relapse. Determination of FLCs is also important, when evaluating new therapeutic substances, and for detection of prognostic patterns for better risk-based stratification of treatment.

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Effect of Proinflammatory Cytokines (IL-6, TNF-α, and IL-1β) on Clinical Manifestations in Indian SLE Patients

Mediators of Inflammation ◽

10.1155/2014/385297 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 43

Author(s):

Vinod Umare ◽

Vandana Pradhan ◽

Milind Nadkar ◽

Anjali Rajadhyaksha ◽

Manisha Patwardhan ◽

...

Keyword(s):

Proinflammatory Cytokines ◽

Lupus Erythematosus ◽

Clinical Manifestations ◽

Serum Levels ◽

Organ Damage ◽

Inactive Disease ◽

Control Group ◽

Healthy Controls ◽

Sledai Score ◽

Active Disease

Systemic lupus erythematosus (SLE) is an inflammatory rheumatic disease characterized by production of autoantibodies and organ damage. Elevated levels of cytokines have been reported in SLE patients. In this study we have investigated the effect of proinflammatory cytokines (IL-6, TNF-α, and IL-1β) on clinical manifestations in 145 Indian SLE patients. One hundred and forty-five healthy controls of the same ethnicity served as a control group. Clinical disease activity was scored according to SLEDAI score. Accordingly, 110 patients had active disease and 35 patients had inactive disease. Mean levels of IL-6, TNF-α, and IL-1βwere found to be significantly higher in SLE patients than healthy controls (P<0.001). Mean level of IL-6 for patients with active disease (70.45±68.32 pg/mL) was significantly higher (P=0.0430) than those of inactive disease patients (43.85±63.36 pg/mL). Mean level of TNF-αwas44.76±68.32 pg/mL for patients with active disease while it was25.97±22.03 pg/mL for those with inactive disease and this difference was statistically significant (P=0.0161). Similar results were obtained for IL-1β(P=0.0002). Correlation between IL-6, TNF-α, and IL-1βserum levels and SLEDAI score was observed (r=0.20,r=0.27, andr=0.38, resp.). This study supports the role of these proinflammatory cytokines as inflammatory mediators in active stage of disease.

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Serum Cortisol and DHEA-S Levels in Schizophrenic Patients with Different Response to Antipsychotic Therapy: Association with Psychopathology / Серумски Концентрации На Кортизол И DHEA-S Кал Пациенти Со Шизофренша Со Различен Одговор На Антипсихотичната Терапша Асоци.1Аци.1А Со Психопатологшата

PRILOZI ◽

10.1515/prilozi-2015-0042 ◽

2015 ◽

Vol 36 (1) ◽

pp. 175-182 ◽

Cited By ~ 1

Author(s):

Zoja Babinkostova ◽

Branislav Stefanovski ◽

Danijela Janicevic-Ivanovska ◽

Valentina Samardziska ◽

Lila Stojanovska

Keyword(s):

Elevated Serum ◽

Serum Cortisol ◽

Control Group ◽

Antipsychotic Treatment ◽

Healthy Controls ◽

Symptom Scale ◽

Antipsychotic Therapy ◽

Schizophrenic Patients ◽

Different Response ◽

Age And Sex

Abstract Background: Previous studies suggested that alterations in serum cortisol and DHEA-S levels may play a role in the pathophysiology of schizophrenia. Imbalance in serum cortisol and DHEA-S levels may be related to responsivity to antipsychotic treatment. Aim: To compare serum cortisol and DHEA-S levels between patients with schizophrenia and healthy controls and to evaluate their association with psychopathology in schizophrenic patients with different response to antipsychotic treatment. Material and Methods: This clinical prospective study included 60 patients with schizophrenia and 40 healthy age and sex matched controls. All patients experienced an acute exacerbation of the illness (PANSS: P1 and P3 ≥ 4). Clinical evaluation of patients was performed using the Positive and Negative Symptom Scale. A questionnaire for socio-demographic and clinical data collection was used. For the purposes of the study, the examined group was divided in two subgroups: responders and nonresponders. Serum cortisol and DHEA-S levels were measuredat baseline in all participants and after 3 and 6 weeks of the antipsychotic treatment in patients with schizophrenia. Results: Patients with schizophrenia had significantly higher serum cortisol and DHEA-S levels comparedwith control group. Responders had significantly higher serum cortisol and DHEA-S levels compared with nonresponders. Responders group had significant correlation between serum cortisol and PANSS positive scale score as well as between hostility and serum DHEA-S. Conclusion: Elevated serum cortisol and DHEA-S levels may play a role in the pathophysiology of schizophrenia. Serum cortisol and DHEA-S are associated with psychopathology in schizophrenic patients with different response to antipsychotic therapy.

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SUBCLINICAL HYPOTHYROIDISM IN ADDISON'S DISEASE

Acta Endocrinologica ◽

10.1530/acta.0.0910674 ◽

1979 ◽

Vol 91 (4) ◽

pp. 674-679 ◽

Cited By ~ 7

Author(s):

Jens Faber ◽

Dorte Cohn ◽

Carsten Kirkegaard ◽

Morten Christy ◽

Kaj Siersbæk-Nielsen ◽

...

Keyword(s):

Addison’S Disease ◽

Mean Value ◽

Normal Serum ◽

Control Group ◽

Addison's Disease ◽

Normal Range ◽

Microsomal Antibody ◽

The Mean ◽

Thyroid Microsomal Antibodies ◽

Serum Tsh

ABSTRACT Fourteen patients with Idiopatic Addison's disease (IAD) were studied in order to detect a possible subclinical hypothyroid state. All were clinically euthyroid with normal serum thyroxine (T4) and serum 3,5′,3′-triiodothyronine (T3). Eleven had circulating thyroid microsomal antibodies in blood. The mean basal serum TSH was significantly higher than that of the control group but only three patients had values above the upper normal range. The mean value of serum T4 was decreased as compared to that of the normal persons, while serum 3,3′,5′-triiodothyronine was elevated. 7.5 mU bovine thyrotrophin per kilogram body weight injected intravenously caused a rise in serum T3 not different from the response in normals. However, as well increasing serum TSH as increasing microsomal antibody titer correlated significantly to decreasing thyroidal release of T3. Our results suggest that clinically euthyroid patients suffering from IAD might have a beginning thyroidal insufficiency because of a progressive immunological damage of the thyroid.

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Clinicopathological features of colorectal polyps and risk of colorectal cancer in acromegaly

Acta Endocrinologica ◽

10.1530/eje-19-0813 ◽

2020 ◽

Vol 182 (3) ◽

pp. 313-318 ◽

Cited By ~ 1

Author(s):

Yorinari Ochiai ◽

Naoko Inoshita ◽

Toshiro Iizuka ◽

Hiroshi Nishioka ◽

Shozo Yamada ◽

...

Keyword(s):

Colorectal Cancer ◽

General Population ◽

Colorectal Polyps ◽

Clinicopathological Features ◽

Control Group ◽

Healthy Controls ◽

Histopathological Features ◽

Increased Risk ◽

Study Participants ◽

Age And Sex

Objective Patients with acromegaly are at increased risk of colorectal polyps. However, their risk of colorectal cancer remains unclear. This study aimed to identify the histopathological features of colorectal polyps in patients with acromegaly and compare their risk of colorectal cancer with that in healthy controls. Methods The study participants were 178 patients who underwent Hardy’s operation and perioperative colonoscopy at our hospital between April 2008 and September 2016. For the control group, we randomly selected 356 age- and sex-matched patients who underwent colonoscopy at our hospital during the same period. The incidence, size, location, and histology of the colorectal polyps detected were compared between the groups. Results Colorectal polyps were detected in 66.8% of the acromegaly group and 24.2% of the control group (P < 0.001). The average number and size of the polyps were 2.44 and 4.74 mm, respectively, in the acromegaly group and 1.77 and 3.89 mm in the control group (P = 0.001). Polyps in the acromegaly group were more likely to be in the rectosigmoid region (P = 0.006). In the acromegaly group, the frequency of polyps ≥5 mm was 34.3% and that for polyps ≥10 mm was 15.2%; the respective values were 7.6% and 2.2% in the control group (P < 0.001). We found no evidence of between-group histopathological differences in the polyp specimens resected by endoscopy. ConclusionsPatients with acromegaly are at an increased risk of colorectal polyps, especially in the rectosigmoid region. However, there is no pathological evidence that they are at greater risk of colorectal cancer than the general population.

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Altered Serum MicroRNA Profile May Serve as an Auxiliary Tool for Discriminating Aggressive Thyroid Carcinoma from Nonaggressive Thyroid Cancer and Benign Thyroid Nodules

Disease Markers ◽

10.1155/2019/3717683 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 4

Author(s):

Aisen Zhang ◽

Cheng Wang ◽

Hui Lu ◽

Xi Chen ◽

Yi Ba ◽

...

Keyword(s):

Thyroid Cancer ◽

Thyroid Carcinoma ◽

Thyroid Nodules ◽

Serum Levels ◽

Mirna Expression Profile ◽

Control Group ◽

Healthy Controls ◽

Benign Nodule ◽

Benign Thyroid Nodules ◽

Benign Thyroid

Thyroid cancers are the most common malignancy of the endocrine system; however, there is no reliable blood biomarkers for thyroid cancer diagnosis and even for aggressive and nonaggressive thyroid cancers as well as benign nodule discrimination. The present study is aimed at evaluating whether circulating microRNA (miRNA) can differentiate aggressive and nonaggressive thyroid cancer from benign thyroid nodules. In this study, we performed a multiphase, case-control study to screen serum miRNA expression profile in 100 patients with papillary thyroid cancer (PTC), 15 patients with aggressive medullary thyroid carcinoma (MTC), 91 patients with benign nodules, and 89 healthy controls using TaqMan low-density array followed by extensive reverse transcription quantitative real-time PCR validation. The results showed that the serum levels of miR-222-3p, miR-17-5p, and miR-451a were markedly increased, while miR-146a-5p, miR-132-3p, and miR-183-3p were significantly decreased in the PTC and benign nodule groups compared with the control group. There was no difference in the miRNA expression profile between the PTC group and the benign nodule group. Nevertheless, the serum levels of miR-222-3p and miR-17-5p were significantly increased in the MTC group than the benign nodule and control group. Moreover, receiver operating characteristic curve analyses demonstrated that the 2 miRNAs and their panel can accurately discriminate MTC from the benign nodule group and healthy controls. These findings indicated that the altered circulating miRNAs may discriminate PTC and benign thyroid nodules from controls, and serum miR-222-3p and miR-17-5p have the potential to serve as auxiliary tools for diagnosing more aggressive thyroid carcinomas, such as MTC.

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