scholarly journals Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rahim Nosrati ◽  
Khalil Abnous ◽  
Mona Alibolandi ◽  
Jafar Mosafer ◽  
Sadegh Dehghani ◽  
...  
Keyword(s):  
Superparamagnetic Iron Oxide ◽  
Growth Effect ◽  
New Horizons ◽  
Theranostic Agent ◽  
Diagnostic Agents ◽  
Magnetic Resonance Imaging Mri ◽  
Diagnostic Agent ◽  
Cancer Agent ◽  
Muc1 Aptamer

AbstractRecently, the siderophores have opened new horizons in nanomedicine. The current study aimed to design a theranostic platform based on superparamagnetic iron oxide nanoparticles-pyoverdine (SPION/PVD) conjugates bound to MUC1 aptamer (MUC1Apt) and loaded with doxorubicin (DOX) as an anti-cancer agent. The SPION/PVD complex was covalently conjugated to MUC1Apt and loaded with DOX to prepare a targeted drug delivery system (SPION/PVD/MUC1Apt/DOX). The investigation of cellular cytotoxicity and uptake of formulations by MTT and flow cytometry in both MUC1 positive (C26) and MUC1 negative (CHO) cell lines revealed that MUC1Apt could improve both cellular uptake and toxicity in the C26 cell line. The evaluation of tumor-targeting activity by in vivo bio-distribution showed that the targeted formulation could enhance tumor inhibitory growth effect and survival rate in C26 tumor-bearing mice. Furthermore, the potential of synthesized SPION/PVD/MUC1Apt/DOX complex as diagnostic agents was investigated by magnetic resonance imaging (MRI) which improved the contrast of tumor site in MRI. Our findings confirm that aptamer-targeted PVD chelated the SPION as a diagnostic agent and loaded with DOX as a chemotherapeutic drug, would be beneficial as a novel theranostic platform.

Folic Acid Functionalized Chlorin e6-Superparamagnetic Iron Oxide Nanocarriers as a Theranostic Agent for MRI-Guided Photodynamic Therapy

2021 ◽  
Vol 17 (2) ◽  
pp. 205-215
Author(s):  
Zhenbo Sun ◽  
Mingfang Luo ◽  
Jia Li ◽  
Ailing Wang ◽  
Xucheng Sun ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Iron Oxide ◽  
Near Infrared ◽  
Biological Fluids ◽  
Superparamagnetic Iron Oxide ◽  
Chlorin E6 ◽  
Theranostic Agent

Imaging-guided cancer theranostic is a promising strategy for cancer diagnostic and therapeutic. Photodynamic therapy (PDT), as an approved treatment modality, is limited by the poor solubility and dispersion of photosensitizers (PS) in biological fluids. Herein, it is demonstrated that superparamagnetic iron oxide (SPIO)-based nanoparticles (SCFs), prepared by conjugated with Chlorin e6 (Ce6) and modified with folic acid (FA) on the surface, can be used as versatile drug delivery vehicles for effective PDT. The nanoparticles are great carriers for photosensitizer Ce6 with an extremely high loading efficiency. In vitro fluorescence imaging and in vivo magnetic resonance imaging (MRI) results indicated that SCFs selectively accumulated in tumor cells. Under near-infrared laser irradiation, SCFs were confirmed to be capable of inducing low cell viability of RM-1 cells In vitro and displaying efficient tumor ablation with negligible side effects in tumor-bearing mice models.

USPIO-positive MS lesions are associated with greater tissue damage than gadolinium-positive-only lesions during 3-year follow-up

2017 ◽  
Vol 24 (14) ◽  
pp. 1852-1861 ◽  
Author(s):  
Anne Kerbrat ◽  
Benoit Combès ◽  
Olivier Commowick ◽  
Adil Maarouf ◽  
Elise Bannier ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Magnetization Transfer ◽  
Superparamagnetic Iron Oxide ◽  
Magnetization Transfer Ratio ◽  
Microstructural Changes ◽  
Ultrasmall Superparamagnetic Iron Oxide ◽  
Mri Scans ◽  
Magnetic Resonance Imaging Mri

Background: Identifying in vivo the processes that determine lesion severity in multiple sclerosis (MS) remains a challenge. Objectives: To describe the dynamics of ultrasmall superparamagnetic iron oxide (USPIO) enhancement in MS lesions and the relationship between USPIO enhancement and microstructural changes over 3 years. Methods: Lesion development was assessed at baseline, Months 3, 6, and 9, using magnetic resonance imaging (MRI) with gadolinium and USPIO. Microstructural changes were assessed at baseline, Months 3, 6, 9, 12, 18, 24, and 36, using relaxometry, magnetization transfer, and diffusion-weighted imaging. Results: We included 15 patients with clinically isolated syndrome. In the 52 MRI scans acquired with USPIO, 22 lesions were USPIO and gadolinium positive, and 44 were USPIO negative but gadolinium positive. Lesions no longer exhibited sustained USPIO enhancement 3 months later. At baseline, lesions that were both USPIO and gadolinium positive had lower magnetization transfer ratio values (common language effect size = 0.84, p = 0.0005) and lower fractional anisotropy values (0.83, p = 0.001) than gadolinium-positive-only lesions. USPIO-positive lesions remained associated with greater damage than gadolinium-positive-only lesions throughout the 3-year follow-up. Conclusion: USPIO enhancement, mainly reflecting monocyte infiltration, is transient and is associated with persistent tissue damage after 3 years.

scholarly journals MRI tracking reveals selective accumulation of stem cell-derived magneto-extracellular vesicles in sites of injury

2019 ◽  
Author(s):  
Zheng Han ◽  
Senquan Liu ◽  
Yigang Pei ◽  
Zheng Ding ◽  
Yuguo Li ◽  
...  
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Kidney Injury ◽  
Induced Pluripotent Stem Cell ◽  
Superparamagnetic Iron Oxide ◽  
Whole Body ◽  
Body Distribution ◽  
Magnetic Resonance Imaging Mri ◽  
Induced Pluripotent

AbstractHuman stem-cell-derived extracellular vesicles (EVs) are currently being investigated for cell-free therapy in regenerative medicine applications, but their biodistribution and tropic properties for homing to injured tissues are largely unknown. Here, we labeled EVs with magnetic nanoparticles to create magneto-EVs that can be tracked by magnetic resonance imaging (MRI). Superparamagnetic iron oxide (SPIO) nanoparticles were coated with polyhistidine tags, which enabled purification of labeled EVs by efficiently removing unencapsulated SPIO particles in the solution. The biodistribution of systemically injected human induced pluripotent stem cell (iPSC)-derived magneto-EV was assessed in three different animal models of kidney injury and myocardial ischemia. Magneto-EVs were found to selectively home to the injury sites and conferred substantial protection in a kidney injury model. In vivo MRI tracking of magnetically labeled EVs represents a new powerful method to assess and quantify their whole-body distribution, which may help optimize further development of EV-based cell-free therapy.

Phosphocholine-decorated superparamagnetic iron oxide nanoparticles: defining the structure and probing in vivo applications

Nanoscale ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 10078-10086 ◽  
Author(s):  
Alessandra Luchini ◽  
Carlo Irace ◽  
Rita Santamaria ◽  
Daniela Montesarchio ◽  
Richard K. Heenan ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Resonance Imaging ◽  
Magnetic Resonance Imaging Mri

Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are performing contrast agents for Magnetic Resonance Imaging (MRI).

scholarly journals SPIO processing in macrophages for MPI: The breast cancer MPI-SNLB-concept

2015 ◽  
Vol 1 (1) ◽  
pp. 558-564
Author(s):  
Dominique Finas ◽  
Stegmann-Frehse Janine ◽  
Sauer Benjamin ◽  
Hüttmann Gereon ◽  
Rody Achim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Culture Medium ◽  
Multiphoton Microscopy ◽  
Superparamagnetic Iron Oxide ◽  
Lymph Node Biopsy ◽  
Magnetic Particle Imaging ◽  
Tumor Mouse Model ◽  
Magnetic Resonance Imaging Mri ◽  
Particle Imaging

AbstractIntroductionBreast cancer (BC) is the most common cancer in women worldwide. We aim to develop a new sentinel lymph node biopsy (SLNB) method with superparamagnetic iron oxide nanoparticles (SPIOs) and magnetic particle imaging (MPI) in BC to avoid tissue damaging while axillary surgery. As we know from i.v. SPIO application in magnetic resonance imaging (MRI), macrophages (MP) are key role player in processing of SPIOs (e.g. in liver) causing a drop of signal intensity. But, knowledge lacks concerning enrichment processes of SPIOs after injection in breast tissue, the adjacent lymphatic tissues and associated cells, especially in BC and metastatic lymph nodes. We already evaluated the distribution of SPIOs in an in vivo healthy and tumor mouse model. Based on these studies we investigate the processing of the SPIOs in MP.Material and MethodsTo evaluate SPIO processing, a mouse MP cell line J774A.1 was incubated either by Resovist in culture medium (RPMI, FBS), or culture medium only as control. MP were than analyzed by transmission electron microscopy (TEM). Additionally, this process was observed in vivo by multiphoton microscopy. Detection of SPIOs was realized by excitation at 1200 nm.ResultsResovist had no toxic effects on cells.MP showed activity in phagocytosis of Resovist after incubation in TEM as well as in multiphoton microscopy. SPIOs were detectable within intracellular vesicles by TEM and 3-photon process. The first cell associated SPIO signal was detected after 1,5 min of incubation by in vivo imaging.ConclusionTo our knowledge this is the first time a 3-photon device was used to image SPIOs in a bio-medical context. System wide scanning is known (MRI, MPI), but nowwe are also able to identify the link to subcellular processing and localization of SPIOs. Further processing of SPIOs in MP is under development.

New Biomimetic Constructs for Improved In Vivo Circulation of Superparamagnetic Nanoparticles

2008 ◽  
Vol 8 (5) ◽  
pp. 2270-2278 ◽  
Author(s):  
Antonella Antonelli ◽  
Carla Sfara ◽  
Luca Mosca ◽  
Elisabetta Manuali ◽  
Mauro Magnani
Keyword(s):  
Blood Circulation ◽  
Superparamagnetic Iron Oxide ◽  
Reticuloendothelial System ◽  
Superparamagnetic Nanoparticles ◽  
Resonance Imaging ◽  
Rapid Clearance ◽  
Body Compartments ◽  
Magnetic Resonance Imaging Mri ◽  
Better Than

Superparamagnetic iron oxide nanoparticles (SPIOs) have been produced and used as a potent and versatile contrast media for magnetic resonance imaging (MRI). Despite a number of efforts to improve their surface chemistry and biocompatibility, the SPIOs half life in blood circulation is very short and they are rapidly taken up by the reticuloendothelial system (RES). In this paper we describe a new method that permits to avoid the rapid clearance of SPIOs. Nanoparticles are made biocompatible by encapsulation into autologous red blood cells. These biomimetic constructs preserve the main properties of the cells that escape RES clearance as well as the properties of the nanoparticles that perform even better than in blood suspension with reduced T2*. These SPIO-loaded RBCs are promising intravascular imaging contrast agents and could also be addressed to selected body compartments by an external magnetic field.

scholarly journals MRI of Monocyte Infiltration in an Animal Model of Neuroinflammation Using SPIO-Labeled Monocytes or Free USPIO

2007 ◽  
Vol 28 (4) ◽  
pp. 841-851 ◽  
Author(s):  
Raoul D Oude Engberink ◽  
Erwin L A Blezer ◽  
Erik I Hoff ◽  
Susanne M A van der Pol ◽  
Annette van der Toorn ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Contrast Enhancement ◽  
Time Window ◽  
Superparamagnetic Iron Oxide ◽  
Inflammatory Lesion ◽  
Contrast Enhanced ◽  
Relaxation Measurements ◽  
Magnetic Resonance Imaging Mri

Magnetic resonance imaging (MRI) has been applied to visualize monocyte infiltration with the use of intravenously injected ultrasmall superparamagnetic iron oxide (USPIO). However, USPIO uptake in vivo remains elusive, and the heterogeneous enhancement patterns observed by MRI point to multiple pathophysiological events. This study focused on specific imaging of monocyte infiltration into the brain by transfusion of superparamagnetic iron oxide (SPIO)-labeled monocytes in a rat model of neuroinflammation, experimentally induced photothrombosis (PT). At day 5 after lesion induction, animals were transfused with SPIO-labeled monocytes (5 × 106 cells) or free USPIO (17 mg Fe/kg). MRI was performed 24, 72 and, 120 h later. To investigate temporal changes directly after intravenous USPIO administration, MRI was performed repeatedly up to 8 h. Relaxation measurements showed that rat monocytes were efficiently labeled in vitro using SPIO ( R2=12±0.9 s−1). After transfusion of SPIO-labeled monocytes, a significant increase in contrast enhanced area (340%±106%) in the PT lesion was observed not before 72 h. Contrast enhancement after USPIO injection increased up to 407%±39% at a much earlier point of time (24 h) and diminished thereafter. Repetitive MRI directly after USPIO injection showed significant contrast enhancement in the lesion within 2 h. Our study shows that MRI enables in vivo tracking of SPIO-labeled monocytes longitudinally. Moreover, our data suggest that contrast enhancement after injection of free USPIO does not primarily represent signals from peripherally labeled monocytes that migrated toward the inflammatory lesion. The use of SPIO-labeled monocytes provides a better tool to specifically assess the time window of monocyte infiltration.

scholarly journals Beyond Oncological Hyperthermia: Physically Drivable Magnetic Nanobubbles as Novel Multipurpose Theranostic Carriers in the Central Nervous System

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2104 ◽  
Author(s):  
Eleonora Ficiarà ◽  
Shoeb Anwar Ansari ◽  
Monica Argenziano ◽  
Luigi Cangemi ◽  
Chiara Monge ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Brain Tumors ◽  
Ad Hoc ◽  
Superparamagnetic Iron Oxide ◽  
Conventional Radiotherapy ◽  
The Central Nervous System ◽  
Magnetic Resonance Imaging Mri ◽  
The Brain

Magnetic Oxygen-Loaded Nanobubbles (MOLNBs), manufactured by adding Superparamagnetic Iron Oxide Nanoparticles (SPIONs) on the surface of polymeric nanobubbles, are investigated as theranostic carriers for delivering oxygen and chemotherapy to brain tumors. Physicochemical and cyto-toxicological properties and in vitro internalization by human brain microvascular endothelial cells as well as the motion of MOLNBs in a static magnetic field were investigated. MOLNBs are safe oxygen-loaded vectors able to overcome the brain membranes and drivable through the Central Nervous System (CNS) to deliver their cargoes to specific sites of interest. In addition, MOLNBs are monitorable either via Magnetic Resonance Imaging (MRI) or Ultrasound (US) sonography. MOLNBs can find application in targeting brain tumors since they can enhance conventional radiotherapy and deliver chemotherapy being driven by ad hoc tailored magnetic fields under MRI and/or US monitoring.

scholarly journals Non-invasive diagnostics in fossils - Magnetic Resonance Imaging of pathological belemnites

2005 ◽  
Vol 2 (2) ◽  
pp. 133-140 ◽  
Author(s):  
D. Mietchen ◽  
H. Keupp ◽  
B. Manz ◽  
F. Volke
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Soft Tissue ◽  
Clinical Diagnostics ◽  
Resonance Imaging ◽  
Non Invasive ◽  
Physiological Processes ◽  
Magnetic Resonance Imaging Mri ◽  
Invasive Diagnostics

Abstract. For more than a decade, Magnetic Resonance Imaging (MRI) has been routinely employed in clinical diagnostics because it allows non-invasive studies of anatomical structures and physiological processes in vivo and to differentiate between healthy and pathological states, particularly of soft tissue. Here, we demonstrate that MRI can likewise be applied to fossilized biological samples and help in elucidating paleopathological and paleoecological questions: Five anomalous guards of Jurassic and Cretaceous belemnites are presented along with putative paleopathological diagnoses directly derived from 3D MR images with microscopic resolution. Syn vivo deformities of both the mineralized internal rostrum and the surrounding former soft tissue can be traced back in part to traumatic events of predator-prey-interactions, and partly to parasitism. Besides, evidence is presented that the frequently observed anomalous apical collar might be indicative of an inflammatory disease. These findings highlight the potential of Magnetic Resonance techniques for further paleontological applications.

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