scholarly journals The Effect Of NaCl Salinity Stress To Phenolic Compound, Total Flavonoid And Antioxidant Activity Of Pegagan (Centella asiatica (L.) Urban) Leaves

2021 ◽  
Vol 41 ◽  
pp. 06004
Author(s):  
Nur Hanifah ◽  
Yekti Asih Purwestri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Salinity Stress ◽  
Defense Mechanism ◽  
Centella Asiatica ◽  
Total Flavonoid ◽  
Nacl Salinity ◽  
Necrotic Symptom ◽  
Dpph Method ◽  
Nacl Concentration

Pegagan (Centella asiatica (L.) Urban) is a herbal plant that contain secondary metabolite compounds like phenol and flavonoid. NaCl salinity is one of abiotic stress that enhanced synthesis of some secondary metabolites in plants. This study was investigated the effect of NaCl salinity stress to phenolic compound, total flavonoid and antioxidant activity of pegagan leaves. Pegagan were treated with five different NaCl concentrations, 0 mM (1), 50 mM (2), 100 mM (3), 150 mM (4) and 200 mM (5) for a week. Morphological leaves were observed for the present of necrotic symptom. Phenolic compound and total flavonoid content were measured using spectrophotometer at wavelength 765 nm and 415 nm. Antioxidant activity was measured based on DPPH method. The result showed that increasing NaCl concentration cause increasing necrotic spot in leaves. Phenolic compound, total flavonoid and antioxidant activity are increased by increasing NaCl concentration. The result indicated that phenol and flavonoid have important role in plant defense mechanism against NaCl toxicity effects.

scholarly journals PENGARUH WAKTU MASERASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban)

2020 ◽  
Vol 9 (4) ◽  
pp. 482
Author(s):  
Ni Komang Trisna Rahayu ◽  
I Dewa Gede Mayun Permana ◽  
GA. Kadek Diah Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Total Flavonoid ◽  
Time Duration ◽  
Time Treatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Effect On Yield

This research aimed to determine the effect of maceration time on the antioxidant activity of pegagan leaf extract and to determine the best maceration time with highest antioxidant activity of pegagan leaf extract. The experimental design used was Completely Randomized Design with maceration time duration there were 18, 24, 30, 36, 42, and 48 hours. All of the treatment repeated three times to obtained 18 units of experiments. The data analyzed by analyze of variance, if the treatment had a significant effect followed by The Duncan Test. The results showed that the maceration time treatment had a significant effect on yield, total phenolik, total flavonoid, total tannin and antioxidant activity of pegagan leaf extract. The 24 hour maceration time was the best treatment with antioxidant activity 66.67%, IC50 632.82 ppm, a yield of 24.30%, a total phenolik of 57.85 mgGAE / g, a total flavonoid of 105.28 mgQE / g. total tannin 54.09 mgTAE / g.

scholarly journals Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

2019 ◽  
Vol 11 (1) ◽  
pp. 49
Author(s):  
Tinta Julianawati ◽  
Hendy Hendarto ◽  
Widjiati Widjiati
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Herbal Medicine ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Peroxyl Radicals ◽  
Chemical Components ◽  
Dpph Method ◽  
Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

scholarly journals THE QUALITY PARAMETERS, TOTAL FLAVONOIDS DETERMINATION AND ANTIOXIDANT ACTIVITY COMPOUND OF ANDALIMAN FRUIT ANDALIMAN FRUIT (ZANTHOXYLUM ACANTHOPODIUM DC.) EXTRACT

2021 ◽  
pp. 34-40
Author(s):  
YUNAHARA FARIDA ◽  
WIDI AZELA ◽  
MEGA ELOK LESTAR ◽  
DIAH KARTIKA PRATAMI
Keyword(s):  
Antioxidant Activity ◽  
Methyl Palmitate ◽  
Ethanol Extract ◽  
Quality Parameters ◽  
Total Flavonoid ◽  
Gas Chromatography Mass Spectrometry ◽  
Uv Spectrophotometry ◽  
Benzenedicarboxylic Acid ◽  
Ic50 Value ◽  
Dpph Method

Objective: The aim of this study was to investigate the chemical compound of the antioxidant activity of the extract from andaliman fruit (Zanthoxylum acanthopodium DC.) and the quality parameters and the total flavonoid determination of the highest antioxidant activity. Methods: The andaliman fruits were extracted using a solvent such as n-hexane, ethyl acetate and 70% ethanol obtained extracts, follow the antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The quality parameters and total flavonoid determination were performed from highest antioxidant activity. Fractionated was done using vacuum liquid chromatography (VLC) with the mobile phase chloroform-methanol (9:1 ~1:9). Purification with Thin Layer Chromatography (TLC) preparative, identification using UV-spectrophotometry, FTIR, Gas chromatography Mass Spectrometry (GCMS) analysis. Results: The 70% of ethanol has the highest antioxidant activity with IC50 value 84.11±0.47 ppm; the quality parameters in this study meet the quality requirements, both specific and nonspecific parameters. Total flavonoid content of the 70% ethanol extract was 2.37±0.02%. The results of the GCMS studies showed the presence of 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone. Conclusion: The results indicate that the 70% ethanol extracts of andaliman fruits contains 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone have antioxidant activity with IC50 value 51.35 ppm.

scholarly journals Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

2019 ◽  
Vol 11 (1) ◽  
pp. 49
Author(s):  
Tinta Julianawati ◽  
Hendy Hendarto ◽  
Widjiati Widjiati
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Herbal Medicine ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Peroxyl Radicals ◽  
Chemical Components ◽  
Dpph Method ◽  
Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

scholarly journals Influence of Harvest Time on Biologically Active Compounds and the Antioxidant Activity in Leaves of Mulberry Grown in Lithuania

2017 ◽  
Vol 45 (2) ◽  
pp. 431-436 ◽  
Author(s):  
Dovilė LEVICKIENĖ ◽  
Elvyra JARIENĖ ◽  
Marek GAJEWSKI ◽  
Honorata DANILČENKO ◽  
Nijolė VAITKEVIČIENĖ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Bioactive Compounds ◽  
Antiradical Activity ◽  
Biologically Active ◽  
Total Flavonoid ◽  
Harvest Time ◽  
Mulberry Leaves ◽  
Dpph Method ◽  
Wide Range

The aim of this study was to establish the influence of harvest time on the contents of flavonoid compounds (rutin, isoquercetin, nicotiflorin, and astragalin) and chlorogenic acid, as well as the antiradical activity in white mulberry (Morus alba L.) leaves grown in Lithuania. Mulberry leaves contain a wide range of bioactive compounds, such as flavonoids and phenolic acids, which are responsible for beneficial effects on human health. Leaves from two mulberry cultivars were collected from July to September in 2015-2016. Quantitative determinations of four flavonoids and chlorogenic acid were conducted by HPLC method and antiradical activity using the 2.2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The results showed that the total flavonoid contents of mulberry leaves in the two cultivars ranged from 921.92 to 1512.02 mg 100 g−1 (dry matter). The highest accumulated rutin, nicotiflorin, and chlorogenic acid contents and the greatest antioxidant activity were found in the leaves of ‘Plodovaja 3’. The bioactive compounds in the leaves of the mulberry cultivars varied over a period of time, where ‘Turchanka’ and ‘Plodovaja 3’ accumulated the highest total flavonoid contents in September and August, respectively. In both cultivars, the antiradical activity was highest in September. There were a very strong positive correlations between the antiradical activity as determined using the DPPH method and the chlorogenic acid contents (r = 0.887, p < 0.05) and the isoquercetin contents (r = 0.848, p < 0.05).

scholarly journals Antioxidant Activity and Total Flavonoid of Carica papaya L. Leaves with Different Varieties, Maturity and Solvent

2019 ◽  
Vol 39 (1) ◽  
pp. 54 ◽  
Author(s):  
Fatma Zuhrotun Nisa ◽  
Mary Astuti ◽  
Sofia Mubarika Haryana ◽  
Agnes Murdiati
Keyword(s):  
Antioxidant Activity ◽  
Carica Papaya ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Water Extraction ◽  
Antioxidant Power ◽  
Mature Leaves ◽  
Dpph Method ◽  
Young Leaves ◽  
Ferric Reducing Antioxidant Power

Carica papaya leaves are one of the vegetables consumed by Indonesian people, especially in Java Island. Carica papaya is easy to grow in Indonesia and has many variants, so, Carica Papaya leaves is a local potent to be developed for functional food and nutraceutical. The aim of this study was to investigate antioxidant activity and total flavonoids of Carica papaya leaves with different varieties, maturity and solvent. Carica papaya leaves (CPL) was firstly extracted by methanol to select two CPLs with high antioxidant capacity and total flavonoid. The two selected CPLs were further tested with different ages mainly young and mature leaves. One selected CPL was further tested with different extraction solvents. Antioxidant activity was determined by 2.2 diphenyl-1-picrylhydrazyl, DPPH and Ferric reducing antioxidant power, FRAP. This study used five varieties of Carica papaya leaves, namely Bangkok, California, Purple, Golden and Grendel. The result showed that Golden and Grendel varieties had a higher percentage of radical scavenging property than the others, which was 78.37% and 77.40% by the DPPH method. Grendel and Purple had a higher percentage of radical scavenging property, which was 45.82 and 34.32 mmol/mg. Grendel and Purple had a higher total flavonoid property, which was 50.33 and 46.02 µg/g. Mature leaves had a higher percentage of radical scavenging property than young leaves by DPPH and FRAP methods. Mature leaves had a higher total flavonoid property than young leaves in both Grendel and Purple. Grendel had a higher antioxidant activity and a higher total flavonoid property than Purple. Grendel with water extraction had a higher antioxidant activity by DPPH and FRAP methods. The total flavonoid of Grendel papaya leaves’ extract with water extraction was lower than ethanol 70% and methanol.

scholarly journals Antioxidant activity ethanol extract of gotu kola (Centella asiatica (L.) Urban) with DPPH method (2,2-Diphenyl-1-Pikrilhidrazil)

2020 ◽  
Vol 3 (2) ◽  
pp. 106
Author(s):  
Muhammad Ainul Yahya ◽  
Iif Hanifa Nurrosyidah
Keyword(s):  
Air Pollution ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Antioxidant Compounds ◽  
Test Results ◽  
Ic50 Value ◽  
Dpph Method

Unhealthy lifestyles and air pollution cause the number of free radicals in the body to increase. To protect the body from free radicals, there are antioxidant compounds as an antidote and stabilize free radicals. One of the Indonesian plants that can be used as antioxidants is gotu kola (Centella asiatica (L.) Urban. Objective: This study aims to determine the antioxidant activity of the ethanol extract of gotu kola herb using the DPPH (2,2-Diphenyl-1-pikrilhidrazil) method. with IC50 value. Method: Gotu kola (Centella asiatica (L.) Urban) was extracted by the soxhletation method using 96% ethanol solvent. The testing of antioxidant activity was carried out using the DPPH (2.2 Diphenyl-1-Pikrihydrazil) method. Result: Test results of antioxidant activity The ethanol extract of gotu kola herb showed an IC50 value of 78.20 ppm. Conclusion: This indicated that the ethanol extract of gotu kola herb was included in the criteria for strong antioxidants. 

scholarly journals Analisis Kandungan Fitokimia dan Uji Aktivitas Antioksidan dari Tanaman Lire (Hemigraphis repanda (L) Hall F.)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 36
Author(s):  
Dina E. B. Rompas ◽  
Max R. J. Runtuwene ◽  
Harry S. J. Koleangan
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Plant Extract ◽  
Flavonoid Compound ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Telah dilakukan penelitian mengenai analisis kandungan fitokimia dan aktivitas antioksidan dari ekstrak tanaman lire yang diekstraksi secara maserasi dengan menggunakan pelarut etanol. Analisis total kandungan fitokimia yaitu uji fenolik dengan menggunakan metode Folin-Ciocalteu dan uji flavonoid menggunakan metode AlCl3. Uji antioksidan menggunakan metode DPPH. Berdasarkan hasil pengujian kandungan fenolik dan flavonoid ekstrak etanol tanaman lire, menunjukkan bahwa kandungan fenolik lebih tinggi dibandingkan dengan kandungan flavonoid. Hasil pengujian aktivitas antioksidan menunjukkan nilai IC50 sebesar 108,673 mg/L pada ekstrak etanol tanaman lire.Research have been done to analyze the phytochemical compound and antioxidant activity from lire plant extract which was extracted based on maceration method using the ethanol solvent. The phythochemical compound analysing to determining phenolik using Folin-Ciocalteu method and AlCl3 method for flavonoid test. Antioxidant activity using the DPPH method. Based on the result, the phenolic and flavonoid compound from extract ethanol of lire plants, was showed that phenolic compound more higher than flavonoid compound. The result of this test was showed the value of IC50 is 108.673 mg/L from the lire plant extract.

scholarly journals SENYAWA FENOLIK DARI KULIT BATANG Garcinia cf. cymosa DAN UJI AKTIFITAS ANTIOKSIDAN

2015 ◽  
Vol 5 (1) ◽  
pp. 89
Author(s):  
Yuliar ◽  
Yunazar Manjang ◽  
Syamsul A. Achmad ◽  
Sanusi Ibrahim
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Structure Determination ◽  
H Nmr ◽  
Dpph Method ◽  
Isolation Methods ◽  
Isolated Compound ◽  
C Nmr

 ABSTRACT A Phenolic compound was isolated from the bark of Garcinia cf. cymosa. Isolation methods were used are maseration, fractionation, and chromatography. Structure determination was done using spectroscopy methods including IR, H-NMR, C-NMR, DEPT, COSY, HMQC, and HMBC. The result showed that the isolated compound is (-)epicatechin. Antioxidant activity of isolated compound was determined by DPPH method. Isolated compound has antioxidant activity which has IC50 = 41,8 ppm. Keywords : Phenolic, (-)epicatechin, Garcinia cf. cymosa, DPPH

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