scholarly journals Betaine biosynthesis in a heterologous expression system based on the B12 producer Pseudomonas denitrificans

2020 ◽  
Vol 222 ◽  
pp. 02047
Author(s):  
Yuri Shkryl ◽  
Anton Degtyarenko ◽  
Valeria Grigorchuk ◽  
Larisa Balabanova ◽  
Lyudmila Tekutyeva
Keyword(s):  
Glycine Betaine ◽  
Abiotic Stresses ◽  
Large Scale ◽  
New Technology ◽  
Expression System ◽  
Methyl Groups ◽  
Heterologous Expression System ◽  
Pseudomonas Denitrificans ◽  
Industrial Strains ◽  
Eukaryotic Organisms

Glycine betaine is an important donor of methyl groups in various metabolic processes of the cell and acts as an osmoprotector when exposed to various abiotic stresses in pro- and eukaryotic organisms. Moreover, exogenous application of betaine activates the production of vitamin B12 in industrial strains-producers. In this work, we have developed a new technology for microbiological betaine synthesis that can be used in biotechnology to activate B12 biosynthesis during large-scale fermentation of Pseudomonas denitrificans.

Applications of blockchain in healthcare (Preprint)

2020 ◽  
Author(s):  
Pranav C
Keyword(s):  
Decision Making ◽  
Large Scale ◽  
Review Paper ◽  
New Technology ◽  
Personal Identification ◽  
Healthcare Industry ◽  
Healthcare Organizations ◽  
Paper Briefly ◽  
Blockchain Technology ◽  
Audit Trails

UNSTRUCTURED The word blockchain elicits thoughts of cryptocurrency much of the time, which does disservice to this disruptive new technology. Agreed, bitcoin launched in 2011 was the first large scale implementation of blockchain technology. Also, Bitcoin’s success has triggered the establishment of nearly 1000 new cryptocurrencies. This again lead to the delusion that the only application of blockchain technology is for the creation of cryptocurrency. However, the blockchain technology is capable of a lot more than just cryptocurrency creation and may support such things as transactions that require personal identification, peer review, elections and other types of democratic decision-making and audit trails. Blockchain exists with real world implementations beyond cryptocurrencies and these solutions deliver powerful benefits to healthcare organizations, bankers, retailers and consumers among others. One of the areas where blockchain technology can be used effectively is healthcare industry. Proper application of this technology in healthcare will not only save billions of money but also will contribute to the growth in research. This review paper briefly defines blockchain and deals in detail the applications of blockchain in various areas particularly in healthcare industry.

New technology for large scale electromagnetic levitation melting of metals

2015 ◽  
Vol 51 (1) ◽  
pp. 121-132 ◽  
Author(s):  
S. Spitans ◽  
E. Baake ◽  
B. Nacke ◽  
A. Jakovičs
Keyword(s):  
Large Scale ◽  
New Technology ◽  
Electromagnetic Levitation ◽  
Levitation Melting ◽  
Electromagnetic Levitation Melting

scholarly journals Future Power Train Solutions for Long-Haul Trucks

2021 ◽  
Vol 13 (4) ◽  
pp. 2225
Author(s):  
Ralf Peters ◽  
Janos Lucian Breuer ◽  
Maximilian Decker ◽  
Thomas Grube ◽  
Martin Robinius ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Co2 Emissions ◽  
Value Chain ◽  
Large Scale ◽  
New Technology ◽  
Co2 Reduction ◽  
Road Transport ◽  
Transport Sector ◽  
Long Distance ◽  
Production Of Hydrogen

Achieving the CO2 reduction targets for 2050 requires extensive measures being undertaken in all sectors. In contrast to energy generation, the transport sector has not yet been able to achieve a substantive reduction in CO2 emissions. Measures for the ever more pressing reduction in CO2 emissions from transportation include the increased use of electric vehicles powered by batteries or fuel cells. The use of fuel cells requires the production of hydrogen and the establishment of a corresponding hydrogen production system and associated infrastructure. Synthetic fuels made using carbon dioxide and sustainably-produced hydrogen can be used in the existing infrastructure and will reach the extant vehicle fleet in the medium term. All three options require a major expansion of the generation capacities for renewable electricity. Moreover, various options for road freight transport with light duty vehicles (LDVs) and heavy duty vehicles (HDVs) are analyzed and compared. In addition to efficiency throughout the entire value chain, well-to-wheel efficiency and also other aspects play an important role in this comparison. These include: (a) the possibility of large-scale energy storage in the sense of so-called ‘sector coupling’, which is offered only by hydrogen and synthetic energy sources; (b) the use of the existing fueling station infrastructure and the applicability of the new technology on the existing fleet; (c) fulfilling the power and range requirements of the long-distance road transport.

scholarly journals A genome-scale metabolic model of Saccharomyces cerevisiae that integrates expression constraints and reaction thermodynamics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Omid Oftadeh ◽  
Pierre Salvy ◽  
Maria Masid ◽  
Maxime Curvat ◽  
Ljubisa Miskovic ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Expression System ◽  
Biomass Composition ◽  
Industrial Biotechnology ◽  
Overflow Metabolism ◽  
Cellular Expression ◽  
A Genome ◽  
Wide Range ◽  
Eukaryotic Organisms ◽  
Genome Scale

AbstractEukaryotic organisms play an important role in industrial biotechnology, from the production of fuels and commodity chemicals to therapeutic proteins. To optimize these industrial systems, a mathematical approach can be used to integrate the description of multiple biological networks into a single model for cell analysis and engineering. One of the most accurate models of biological systems include Expression and Thermodynamics FLux (ETFL), which efficiently integrates RNA and protein synthesis with traditional genome-scale metabolic models. However, ETFL is so far only applicable for E. coli. To adapt this model for Saccharomyces cerevisiae, we developed yETFL, in which we augmented the original formulation with additional considerations for biomass composition, the compartmentalized cellular expression system, and the energetic costs of biological processes. We demonstrated the ability of yETFL to predict maximum growth rate, essential genes, and the phenotype of overflow metabolism. We envision that the presented formulation can be extended to a wide range of eukaryotic organisms to the benefit of academic and industrial research.

scholarly journals A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 420
Author(s):  
Yi Ma ◽  
Liu Cui ◽  
Meng Wang ◽  
Qiuli Sun ◽  
Kaisheng Liu ◽  
...  
Keyword(s):  
Large Scale ◽  
Expression System ◽  
High Yield ◽  
Wild Type ◽  
High Quality ◽  
Efficient Protection ◽  
Bacterial Ghosts ◽  
Cell Envelopes ◽  
Extracellular Structures ◽  
First Time

Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-type protein E (EW) from phage ΦX174 and the screened mutant protein E (EM) in the Escherichia coli BL21(DE3) strain. The results show that the lysis efficiency mediated by protein EM was improved. The implementation of the pLysS plasmid allowed nearly 100% lysis efficiency, with a high initial cell density as high as OD600 = 2.0, which was higher compared to the commonly used BG preparation method. The results of Western blot analysis and immunofluorescence indicate that the expression level of protein EM was significantly higher than that of the non-pLysS plasmid. High-quality BGs were observed by SEM and TEM. To verify the applicability of this method in other bacteria, the T7 RNA polymerase expression system was successfully constructed in Salmonella enterica (S. Enterica, SE). A pET vector containing EM and pLysS were introduced to obtain high-quality SE ghosts which could provide efficient protection for humans and animals. This paper describes a novel and commonly used method to produce high-quality BGs on a large scale for the first time.

Modeling and Implementation of Inter-Campuses Equipment Optimized Management Based on WebGIS

2013 ◽  
Vol 680 ◽  
pp. 534-539
Author(s):  
Wei Feng Ma
Keyword(s):  
Information Management ◽  
Spatial Data ◽  
Large Scale ◽  
New Technology ◽  
Rapid Expansion ◽  
Mathematics Model ◽  
Data Mapping ◽  
Geographically Dispersed ◽  
New Research ◽  
Research Challenge

With the rapid expansion of the campus scale and the increasing of the geographically dispersed campus, how to adopt new theory, new method and new technology to realize the equipment optimized assignment and the information management is a new research challenge. It is the key to safeguard the national fund to use reasonably, and to speed up the development of education healthily. Through analyzing the domestic and foreign related research works, the paper proposed that it can take use of the spatial data expression and analysis with Geographic Information System (GIS) to realize the large-scale and inter-campuses equipment optimized assignment and information management. It discussed the mathematics model and the system architecture. Moreover, the paper described the key implementation technology in great detail such as spatial data mapping with MapInfo professional 9 and the development of WebGIS functions with MapXtreme. The results show that the solution is feasible and effective.

Electric Toothbrushes–For Whom are They Designed?

2002 ◽  
Vol 16 (1) ◽  
pp. 6-8 ◽  
Author(s):  
Sebastian Ciancio
Keyword(s):  
Clinical Studies ◽  
Large Scale ◽  
Economic Status ◽  
New Technology ◽  
Ease Of Use ◽  
Special Populations ◽  
Gingival Health

Powered toothbrushes were first introduced on a large scale in the early 1960s. However, because of a clear lack of superiority compared with manual brushes, and problems with mechanical breakdowns, their sales decreased significantly. However, recommendation for their use continued in special populations with dexterity and cognition problems. The 1990s ushered in an era of new technology, and studies began to suggest superiority of some powered brushes, particularly those using oscillating-rotating or counter-rotational actions. Some studies have shown interproximal cleansing abilities superior to those of manual brushes and yielding results similar to those achieved with the use of a manual brush and floss. Both controlled and open-labeled studies have suggested that electric brushes improve gingival health with patients who routinely used manual brushes prior to using these new powered brushes, and safety has been clearly established. In recommending powered toothbrushes, practitioners should familiarize themselves with the products available, with the clinical studies supporting their benefits compared with manual brushes, their safety and ease of use, and the patient's economic status.

Caenorhabditis elegansPlexinA, PLX-1, interacts with transmembrane semaphorins and regulates epidermal morphogenesis

Development ◽  
2002 ◽  
Vol 129 (9) ◽  
pp. 2053-2063 ◽  
Author(s):  
Takashi Fujii ◽  
Fumi Nakao ◽  
Yukimasa Shibata ◽  
Go Shioi ◽  
Eiji Kodama ◽  
...  
Keyword(s):  
Rna Interference ◽  
Morphological Changes ◽  
Expression System ◽  
Transmembrane Proteins ◽  
Axonal Guidance ◽  
Heterologous Expression System ◽  
C Elegans ◽  
Morphological Defects ◽  
Epidermal Morphogenesis ◽  
Null Mutants

The plexin family transmembrane proteins are putative receptors for semaphorins, which are implicated in the morphogenesis of animal embryos, including axonal guidance. We have generated and characterized putative null mutants of the C. elegans plexinA gene, plx-1. plx-1 mutants exhibited morphological defects: displacement of ray 1 and discontinuous alae. The epidermal precursors for the affected organs were aberrantly arranged in the mutants, and a plx-1::gfp transgene was expressed in these epidermal precursor cells as they underwent dynamic morphological changes. Suppression of C. elegans transmembrane semaphorins, Ce-Sema-1a and Ce-Sema-1b, by RNA interference caused a displacement of ray 1 similar to that of plx-1 mutants, whereas mutants for the Ce-Sema-2a/mab-20 gene, which encodes a secreted-type semaphorin, exhibited phenotypes distinct from those of plx-1 mutants. A heterologous expression system showed that Ce-Sema-1a, but not Ce-Sema-2a, physically bound to PLX-1. Our results indicate that PLX-1 functions as a receptor for transmembrane-type semaphorins, and, though Ce-Sema-2a and PLX-1 both play roles in the regulation of cellular morphology during epidermal morphogenesis, they function rather independently.

Fits and Starts: A Current Look at Marine and Industrial Gas Turbine Electric Start Systems

2017 ◽  
Author(s):  
Glenn McAndrews
Keyword(s):  
Gas Turbine ◽  
Gas Turbines ◽  
Life Cycle Cost ◽  
Large Scale ◽  
Past History ◽  
New Technology ◽  
Cost Benefit ◽  
New Production ◽  
Intense Activity ◽  
Industrial Gas Turbine

Electric starter development programs have been the subject of ASME technical papers for over two decades. Offering significant advantages over hydraulic or pneumatic starters, electric starters are now poised to be the preferred choice amongst gas turbine customers. That they are not now the dominant starter in the field after decades of investment and experimentation is attributable to many factors. As with any new technology, progress is often unsteady, depending on budgets, market conditions, customer buy-in, etc. Additionally, technological advances in the parent technologies, in this case electric motors, can abruptly and rapidly change, further disturbing the best laid introduction plans. It is therefore not too surprising that only recently, is the industry beginning to see the deployment of electric starters on production gas turbines. The earliest adoption occurred on smaller gas turbine units, generally less than 10 MW in power. More recently, gas turbines greater than 10 MWs are being sold with electric starters. The authors expect that regardless of their size or fuel supply, most all future gas turbine users will opt for electric starters. This may even include the “larger” frame machines with power greater than 100 MW. Starting with some past history, this paper will not only summarize past development efforts, it will attempt to examine the current deployment of electric starters throughout the marine and industrial gas turbine landscapes. The large-scale acceptance of electric start systems for both new production and retrofit will depend on the favorable cost/benefit assessment when weighing both first cost and life cycle cost. The current and intense activity in electric vehicle applications is giving rise to even more power dense motors. The paper will look at some of these exciting applications, the installed products, and the technologies behind the products. To what extent these new products may serve the needs of the gas turbine community will be the central question this paper attempts to answer.

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