scholarly journals The effect of organosolv pretreatment on optimization of hydrolysis process to produce the reducing sugar

2018 ◽  
Vol 154 ◽  
pp. 01022 ◽  
Author(s):  
Fibrillian Zata Lini ◽  
Tri Widjaja ◽  
Nuniek Hendrianie ◽  
Ali Altway ◽  
Siti Nurkhamidah ◽  
...  

As the fossil energy decrease such as petroleum and natural gas, that are encourages a lot of research to develop new sources of energy from renewable raw materials. One of the source is through reducing sugar (glucose and xylose) obtained from coffee pulp waste; this is due to abundant production of coffee pulp every year reaching 743 kg/ha. In addition, this waste has not been used optimally and the cellulose and hemicellulose content of the coffee is high. The purpose of this study is to get the optimal operating condition for reducing sugar production from coffee pulp waste. The method used for optimization is Response Surface Methodology with Central Composite Design. The optimum operation condition obtained was pH 4.63 at 34ºC for 16.29 hours of hydrolysis. As a result, the predicted yield gained was 0.147 grams of reducing sugars / gram of cellulose+hemicellulose. The result indicates the gained yield was 0.137 grams of reducing sugars / gram of cellulose+hemicellulose.

2019 ◽  
Vol 10 (3) ◽  
pp. 453
Author(s):  
Toto Iswanto ◽  
Nuniek Hendrianie ◽  
Maya Shovitri ◽  
Ali Altway ◽  
Tri Widjaja

2015 ◽  
Vol 3 ◽  
pp. 455-460
Author(s):  
Victor Panfilov ◽  
Boris Karetkin ◽  
Mariya Gordienko ◽  
Irina Shakir

Biotechnological methods have perhaps the greatest potential in deep processing of renewable raw materials. Bioconversion of lignocelluloses materials substantially increases its bioavailability and effectiveness of consumption by microorganisms. The enzymatic and chemical hydrolysis of wheat bran under mild conditions are compared in this study.The purpose of this study was to define microbiological and biochemical criteria for the bioconversion. Enzymatic hydrolysis of extruded bran was held with three enzyme preparations usually used in the conversion of vegetable raw materials: “Viscoferm,” “Cellic HTec2,” and “Celloviridin G20x.” The central composite design was used for parameter optimization. Saccharomyces cerevisiae yeast was used for assessment of biological value of the slurry obtained. The content of crude protein of the product obtained was 1.9 times higher than the initial.


2021 ◽  
Vol 10 (4) ◽  
pp. 147-153
Author(s):  
N. A. Dyakova ◽  
A. I. Slivkin ◽  
S. P. Gaponov ◽  
E. A. Bobina ◽  
L. A. Shishorina

Introduction. Small-leaved linden flowers are mainly used for aquatic extracts, and their pharmacological benefit is based on water-soluble polysaccharides.Aim. The aim of this study was to investigate the rate of accumulating all reducing sugars in small-leaved linden flowers, collected in agro- and urbo-cenoses of the Voronezh region.Materials and methods. 51 sites were selected for collecting flowers from the small-leaved linden, which is a widespread deciduous tree species in Russia. In order to determine the total level of reducing sugars in the samples, we measured glucose levels using the method described in Pharmacopeia article "Linden Flower". Correlation coefficients were analyzed to examine in detail the effect of the major pollutants (heavy metals and arsenic) on the accumulation of reducing sugars in small-leaved linden flowers.Results and discussion. All analyzed samples of medicinal plant raw materials were benign in terms of their reducing sugar levels. Samples collected in control (protected) areas contained reducing sugar levels 13.31 to 16.89 %, which is 6–8 times more than the lower numerical value established by the Pharmacopoeia article. In the agrocenoses of the region, the concentration of reducing sugars varied from 6.12 to 16.68 %, which is 3–8 times more than the value given in the Pharmacopoeia article. In the urbocenoses of the region, a lower level of reducing sugars was found compared to samples from protected areas (2.35–13.49 %). Correlation coefficients showed a noticeable negative impact of cadmium, chromium, cobalt, copper, zinc, as well as a moderate negative effect of lead and mercury on the accumulation of reducing sugars in the small-leaved linden flowers.Conclusion. The lowest concentrations of reducing sugars were detected in samples harvested along the streets of large cities in the region, and along highways, roads and railways. This suggests that anthropogenic factors might negatively impact the accumulation of reducing sugars in small-leaved linden flowers in these areas. At the same time, it is possible that saccharide complexes might have reacted with heavy metals instead of the complexing agent when levels of reducing sugars in the samples were quantitatively determined. This would have the effect of underrepresenting reducing sugar levels in the raw materials.


2018 ◽  
Vol 164 ◽  
pp. 01039 ◽  
Author(s):  
Roy Hendroko Setyobudi ◽  
Satriyo Krido Wahono ◽  
Praptiningsih Gamawati Adinurani ◽  
Ahmad Wahyudi ◽  
Wahyu Widodo ◽  
...  

The huge amount of coffee pulp waste is an environmental problem. Anaerobic fermentation is one of the alternative solutions. However, availability of coffee pulp does not appear for year-round, whereas biogas needs continuous feedstocks for digester stability. This research uses coffee pulp from Arabica Coffee Factory at Mengani, Kintamani, Bali–Indonesia. The coffee pulp was transformed into coffee pulp-hay product by sun drying for preservations to extend the raw materials through the year. Characterization of coffee pulp-hay was conducted after to keep for 15 mo for review the prospect as biogas feedstocks. Several parameters were analyzed such as C/N ratio, volatile solids, carbohydrate, protein, fat, lignocellulose content, macro-micro nutrients, and density. The review results indicated that coffee pulp-hay is prospective raw material for biogas feedstock. This well-proven preservation technology was able to fulfill the continuous supply. Furthermore, some problems were found in the recent preliminary experiment related to the density and fungi growth in the conventional laboratory digester. Further investigation was needed to implement the coffee pulp – hay as biogas feedstocks.


2014 ◽  
Vol 3 (2) ◽  
pp. 15-18
Author(s):  
Zulqarnain Albaasith ◽  
Rahmad Nauli Lubis ◽  
Rondang Tambun

Glucose syrup is a trade name of hydrolysis of starch solution. The purpose of this research is to produce glucose syrup from banana peel and examine the variables that affect the hydrolysis process. The process of making glucose syrup in this study through a series of treatment until the resulting slurry is ready to be processed banana peel. Hydrolysis process is then performed using α-amylase and glucoamylase enzyme. This process is carried out in four phases: liquefaction, saccharification, purification and concentration. In the liquefaction process to vary the weight of raw materials: water (10% w / v, 20% w / v, 30% w / v and 40% w / v) and heating temperature (60oC, 70oC, 80oC and 90oC). In this research shows that the best glucose syrup is in the variation of glucose syrup raw materials: water 10% w / v at a temperature of 90oC which produces reducing sugar 9.86% and 98.56% DE value.


Processes ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1734
Author(s):  
Christian Appl ◽  
Frank Baganz ◽  
Volker C. Hass

Enzymatic hydrolysis processes can be used to produce organic nutrient media from renewable raw materials. However, many of these processes are not optimally designed, so expensive enzymes and substrates are wasted. Mathematical models and Digital Twins (DTs) are powerful tools, which can be used to optimize bioprocesses and, thus, increase the yield of the desired products. Individual enzymatic hydrolysis processes have already been modeled, but models for the combined starch hydrolysis and proteolysis, or DTs, are not available yet. Therefore, an easily adaptable, dynamic, and mechanistic mathematical model representing the kinetics of the enzymatic hydrolysis process of the combined starch hydrolysis and proteolysis was developed and parameterized using experimental data. The model can simulate the starch hydrolysis process with an agreement of over 90% and the proteolysis process with an agreement of over 85%. Subsequently, this model was implemented into an existing DT of a 20 L stirred tank reactor (STR). Since the DT cannot only map the kinetics of the enzymatic process, but also the STR with the associated periphery (pumps, heating jacket, etc.), it is ideally suited for future process control strategy development and thus for the optimization of enzymatic hydrolysis processes.


2020 ◽  
pp. 48-55
Author(s):  
M.E. Sharanda ◽  
◽  
E.A. Bondarenko ◽  

Ethylene glycol and propylene glycol are important representatives of polyols. On an industrial scale, they are obtained from petrochemical raw materials. Within a decade, significant efforts were made for the producing of polyols from biologically renewable raw materials - carbohydrates. The general trend for carbohydrate hydrogenolysis includes application of liquid-phase process with the use of modified metal-oxide catalysts, at 120-120 ° C and pressure of 3MPa or above. So high pressure is used for the reason to increase hydrogen solubility, and also due to the high partial pressure of low boiling solvents. We supposed that usage of high boiling solvents could allow hydrogenolysis to be performed at the lower pressure. Ethylene glycol and propylene glycol are of particular interest as such kind of solvent since they are both the main products of glucose hydrogenolysis. In this work, the process of hydrogenolysis of glucose and fructose over Cu / MgO-ZrO2 catalyst have been studied at temperature range of 160-200 °C and a pressure of 0.1-0.3 MPa in a flow reactor. The solvents were simultaneously the target products of the reaction - ethylene glycol and / or propylene glycol. Gas chromatography and 13C NMR were used for the reaction products identification. It was found that the solubility of glucose in propylene glycol is 21 % by weight, and in ethylene glycol 62% by weight. It was pointed out that the process of hydrogenolysis can take place at a pressure close to atmospheric. Under these conditions, the conversion of hexoses reaches 96-100 %. The reaction products are preferably propylene glycol and ethylene glycol. The total selectivity for C3-2 polyols is 90-94 %, that is higher than in the hydrogenolysis of glucose in aqueous solution.


2020 ◽  
Vol 164 ◽  
pp. 08031
Author(s):  
Evgeniy Velichko ◽  
Edward Tshovrebov ◽  
Ural Niyazgulov

The article deals with issues of monitoring, planning, organizational and technical support, economic regulation and improving the efficiency of the infrastructure for processing, recycling and disposal of waste, resource conservation and management of secondary resources, their use as secondary raw materials for production, services, work and power generation. The Russian Federation has significant potential for economic growth due to the efficient use of secondary resources from billions of tons of generated production and consumption waste annually. These ecologically unsafe anthropogenic objects can be characterized as a source of valuable renewable raw materials, material and fuel and energy resources. However, the scale and level of use of various types of secondary resources are characterized by considerable unevenness and depend on the demand for secondary raw materials, the resource value of the waste, the environmental situation arising from treating them as environmental polluters, on the real economic conditions that determine the profitability of each specific type. economic activities that use secondary resources for the manufacture of products, works, services, energy production. The methods of monitoring the industry for the treatment, disposal and disposal of waste are: information-analytical, information-statistical, sociological, geo-information. The legal status of the monitoring system of the industry for the treatment, disposal and disposal of waste is determined by the limitations of its functionality within the framework of the goals and objectives facing it.


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