Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract

Planta Medica ◽  
2021 ◽  
Author(s):  
Sandra Alves de Sousa Garcia ◽  
Priscila Bianca Rodrigues da Rocha ◽  
Bruno dos Santos Souza ◽  
Andressa Tuane Santana Paz ◽  
Ana Luiza Caetano Negris ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Skin Permeation ◽  
Sol Gel ◽  
Extraction Procedure ◽  
Average Diameter ◽  
Skin Aging ◽  
Chemical Degradation ◽  
Vertical Diffusion ◽  
Flowing Liquid ◽  
Span 80

AbstractPunicalagin, the principal ellagitannin of Lafoensia pacari leaves, has proven antioxidant activity, and standardized extracts of L. pacari can be topically used for skin aging management. We hypothesized that Pluronic nanomicelles or vesicles could solubilize sufficiently large amounts of the standardized extracts of L. pacari and provide chemical stability to punicalagin. The standardized extracts of L. pacari were obtained with an optimized extraction procedure, and the antioxidant activity was characterized. Formulations containing Pluronic at 25% and 35% were obtained with or without Span 80. They were characterized by average diameter, polydispersity index, punicalagin content, physicochemical stability, and rheology. A release and skin permeation study was carried out in vertical diffusion cells. The extraction procedure allowed quantifying high punicalagin content (i.e., 141.61 ± 3.87 mg/g). The standardized extracts of L. pacari showed antioxidant activity for all evaluated methods. Pluronic at 25 and Pluronic at 35 with standardized extracts of L. pacari showed an average diameter of about 25 nm. The addition of Span 80 significantly increased the mean diameter by 15-fold (p < 0.05), indicating the spontaneous formation of vesicles. Pluronic formulations significantly protected punicalagin from chemical degradation (p < 0.05). Pluronic at 25 formulations presented as free-flowing liquid-like systems, while Pluronic at 35 resulted in an increase of about 44-fold in |ƞ*|. The addition of Span 80 significantly reduced the Pluronic sol-gel transition temperature (p < 0.05), indicating the formation of vesicles. Formulations with Span 80 significantly enhanced punicalagin skin permeation compared to formulations without Span 80 (p < 0.05). Formulations with Span 80 were demonstrated to be the most promising formulations, as they allowed significant permeation of punicalagin (about 80 to 315 µg/cm2), which has been shown to have antioxidant activity.

Preparation and Antimicrobial Activity of N-(2-hydroxy-3-trimethylamine) Propyl Chitosan Chloride Nanoparticles

2014 ◽  
Vol 685 ◽  
pp. 55-58
Author(s):  
Yan Hua Lu ◽  
Jia Qi Wang
Keyword(s):  
Main Chain ◽  
Sodium Tripolyphosphate ◽  
Sol Gel ◽  
Average Diameter ◽  
Low Molecular Weight ◽  
Amino Group ◽  
Step Method ◽  
Ionic Bonds ◽  
Span 80 ◽  
High Degree

N-(2-hydroxy-3-trimethylamine) propyl chitosan chloride (HTCC) nanoparticles were prepared through two-step method using chitosan with low molecular weight and high degree of deacetylation. H of-NH2of chitosan was replaced with quaternary ammonium salt groups to form HTCC. HTCC was further modified by ion sol-gel reaction with sodium tripolyphosphate, the reaction can be in the dispersion under the action of Span-80, and the ionic bonds formed between phosphate group of sodium tripolyphosphate and the amino group in HTCC main chain, well dispersed HTCC nanoparticles with a average diameter of 23.6nm were than obtained. The structure was characterized with fourier transform infrared spectrum (FTIR). When HTCC nanoparticles were applied to contact withstaphylococcus aureus(S.aureus) andescherichia coli(E.coli), the results showed completely under the condition of antimicrobial resistance.

Efficient Micromixing for Continuous Biodiesel Production from Jatropha Oil

2018 ◽  
Vol 9 (1) ◽  
pp. 133-139
Author(s):  
Waleed S. Mohammed ◽  
Ahmed H. El-Shazly ◽  
Marwa F. Elkady ◽  
Masahiro Ohshima
Keyword(s):  
Methyl Esters ◽  
Continuous Process ◽  
Sol Gel ◽  
Average Diameter ◽  
Biodiesel Production ◽  
High Mass ◽  
Molar Ratio ◽  
Jatropha Oil ◽  
Energy Deficiency ◽  
Gel Preparation

Introduction: The utilization of biodiesel as an alternative fuel is turning out to be progressively famous these days because of worldwide energy deficiency. The enthusiasm for utilizing Jatropha as a non-edible oil feedstock is quickly developing. The performance of the base catalyzed methanolysis reaction could be improved by a continuous process through a microreactor in view of the high mass transfer coefficient of this technique. Materials & Methods: Nanozirconium tungstovanadate, which was synthetized using sol-gel preparation method, was utilized in a complementary step for biodiesel production process. The prepared material has an average diameter of 0.066 &µm. Results: First, the NaOH catalyzed methanolysis of Jatropha oil was investigated in a continuous microreactor, and the efficient mixing over different mixers and its impact on the biodiesel yield were studied under varied conditions. Second, the effect of adding the nanocatalyst as a second stage was investigated. Conclusion: The maximum percentage of produced methyl esters from Jatropha oil was 98.1% using a methanol/Jatropha oil molar ratio of 11 within 94 s using 1% NaOH at 60 &°C. The same maximum conversion ratio was recorded with the nanocatalyst via only 0.3% NaOH.

scholarly journals Anti-Aging Potential of Extracts from Washingtonia filifera Seeds

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 151
Author(s):  
Benedetta Era ◽  
Sonia Floris ◽  
Valeria Sogos ◽  
Clara Porcedda ◽  
Alessandra Piras ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Pharmaceutical Preparation ◽  
Human Keratinocytes ◽  
Inhibitory Effect ◽  
Skin Aging ◽  
Stressed Cells ◽  
Collagenase Inhibition ◽  
Washingtonia Filifera ◽  
Seed Extracts

The aim of this study was to test the inhibitory effect of fruit extracts from Washingtonia filifera on skin aging-related enzymes. The pulp extracts did not exert a significant enzyme inhibition while seed extracts from W. filifera exhibit anti-elastase, anti-collagenase, and anti-tyrosinase activities. Tyrosinase was mildly inhibited while a stronger effect was observed with respect to elastase and collagenase inhibition. Alcoholic extracts provided better results than aqueous extracts. Among them, methanol extracts showed the prominent enzyme inhibitory activities being IC50 value for elastase and collagenase comparable and even better than the reference compound. The inhibition mode of the most active extracts was investigated by Lineweaver-Burk plot analysis. Seed extracts from W. filifera were also investigated for their photo-protective effect by Mansur equation and the antioxidant activity of W. filifera extract was evaluated in oxidative-stressed cells. To evaluate the safety of the extract, the effect on cell viability of human keratinocytes cells was analyzed. Methanol extract presented the best photo-protective effect and exerted an antioxidant activity in a cellular system with no cytotoxic effect. The overall results demonstrate that W. filifera extracts are promising sources of bioactive compounds that could be used in cosmetic and pharmaceutical preparation.

scholarly journals Development of Novel Oral Formulations of Disulfide Antioxidants Based on Porous Silica for Controlled Release of the Drugs

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 963
Author(s):  
Ekaterina S. Dolinina ◽  
Elena V. Parfenyuk
Keyword(s):  
Antioxidant Activity ◽  
Surface Chemistry ◽  
Sol Gel ◽  
Porous Silica ◽  
The Body ◽  
Oral Drug ◽  
Therapeutic Concentration ◽  
Release Process ◽  
Zero Order Kinetics ◽  
Long Time

Powerful antioxidant α-lipoic acid (LA) exhibits limited therapeutic efficiency due to its pharmacokinetic properties. Therefore, the purpose of this work was to evaluate the ability of silica-based composites of LA as well as its amide (lipoamide, LM), as new oral drug formulations, to control their release and maintain their therapeutic concentration and antioxidant activity in the body over a long time. The composites synthesized at different sol–gel synthesis pH and based on silica matrixes with various surface chemistry were investigated. The release behavior of the composites in media mimicking pH of digestive fluids (pH 1.6, 6.8, and 7.4) was revealed. The effects of chemical structure of the antioxidants, synthesis pH, surface chemistry of the silica matrixes in the composites as well as the pH of release medium on kinetic parameters of the drug release and mechanisms of the process were discussed. The comparative analysis of the obtained data allowed the determination of the most promising composites. Using these composites, modeling of the release process of the antioxidants in accordance with transit conditions of the drugs in stomach, proximal, and distal parts of small intestine and colon was carried out. The composites exhibited the release close to the zero order kinetics and maintained the therapeutic concentration of the drugs and antioxidant effect in all parts of the intestine for up to 24 h. The obtained results showed that encapsulation of LA and LM in the silica matrixes is a promising way to improve their bioavailability and antioxidant activity.

Effect of pH Value on the Synthesis of NiO Nanoparticles and Microstructure of NiO Based Composite Anode

2021 ◽  
Vol 317 ◽  
pp. 447-453
Author(s):  
Noor Hidayah Aniza Zakaria ◽  
Nafisah Osman
Keyword(s):  
Ph Value ◽  
Sol Gel ◽  
Secondary Phase ◽  
Average Diameter ◽  
Morphological Observation ◽  
Nio Nanoparticles ◽  
Composite Anode ◽  
Ph Values ◽  
Particle Size Analyzer ◽  
Nio Nps

NiO nanoparticle was synthesized by a sol-gel method with three different pH values namely pH=1, 7 and 11, and then calcined at temperature of 450 ᵒC. The influence of different pH values on the physical properties of NiO nanoparticles were investigated by a particle size analyzer (PSA), field emission scanning electron microscope (FESEM) and X-ray diffractometer (XRD). Structural analysis confirmed that a cubic structure of NiO nanoparticle was obtained without any secondary phase for NiO powders prepared with pH=1, while the peak of secondary phase (Ni) appeared for NiO powders prepared with pH= 7 and 11. Morphological observation showed that the NiO nanoparticles prepared with pH=7 and 11 tend to form more agglomerates compared to one prepared with pH=1. The average diameter of NiO nanoparticles with pH 1, 7 and 11 were approximately in the range of 19-26 nm, 21-28 nm, and 24-30 nm, respectively. NiO powder that was synthesized with pH=1 was further used to prepare composite anode of NiO Nps-BaCe0.54Zr0.36Y0.1O2.95 (BCZY) powder. Unfortunately a composite of NiO Nps-BaCeO3-BaZrO3 was obtained instead of BCZY and governed by agglomerates with size in the range of 70-300 nm.

scholarly journals Synthesis and Optical Manifestation of NiO-Silica Nanocomposite

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
S. Chakrabarty ◽  
K. Chatterjee
Keyword(s):  
Sol Gel ◽  
Size Variation ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Peak Position ◽  
Band Gap Energy ◽  
Strong Emission ◽  
Silica Nanocomposites ◽  
Composite Formation ◽  
Silica Nanocomposite

NiO-silica nanocomposites with average diameter ranging from 2–40 nm were prepared by sol-gel method followed by the heat treatment varying from 400°C to 1000°C. The details of morphology and crystalline nature of all the as prepared samples were characterized by TEM, HRTEM, and XRD analysis. The planes obtained from SAED pattern supports the planes originated from XRD study. From the optical absorption study, it is revealed that the band gap energy of NiO can extensively be manipulated by composite formation with silica and the size variation of that nanocomposite. Absorption peak position varies almost linearly with the oxidation temperature of the samples. Photoluminescence spectroscopy reveals that NiO-silica nanocomposite, prepared at 600°C and below, shows strong emission at 3.62 eV, but the nanocomposites with bigger size greatly hinder the effect of selective emissivity.

scholarly journals Optimization of HIB Value Combination of Tween 60 and Span 80 on Cream Formulation of Ethanol Extract of Green Tea Leaves (Camellia Sinensis L.)

2018 ◽  
Vol 23 (3) ◽  
pp. 124
Author(s):  
Fransiska Lisa Anindya Putri ◽  
Akhmad Kharis Nugroho ◽  
Erna Prawita Setyowati
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Lattice Design ◽  
Dpph Method ◽  
Green Tea Leaves ◽  
Span 80 ◽  
Tween 60

Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.

scholarly journals SYNTHESIS OF ENCAPSULATED CHROMOLAENA ODORATA LEAF EXTRACT IN CHITOSAN NANOPARTICLE BY USING IONIC GELATION METHOD AND ITS ANTIOXIDANT ACTIVITY

2021 ◽  
pp. 112-115
Author(s):  
KENI IDACAHYATI ◽  
WINDA TRISNA WULANDARI ◽  
FIRMAN GUSTAMAN AND INDRA INDRA
Keyword(s):  
Antioxidant Activity ◽  
Zeta Potential ◽  
Leaf Extract ◽  
Crosslinking Agent ◽  
Average Diameter ◽  
Ionic Gelation ◽  
Chromolaena Odorata ◽  
Dpph Method ◽  
Chitosan Nanoparticle

Objective: The aim of this study was to determine the antioxidant activity of Chromolaena odorata. Methods: Encapsulation of Chromolaena odorata leaf extract by nano chitosan was synthesized by using chitosan and NaTPP as the crosslinking agent. The antioxidant activity was conducted by using the DPPH method. Results: Nanoparticles of Chromolaena odorata leaf extract has an average diameter of 675±218 nm and+23.4±7.14 mV of zeta potential. The antioxidant activity of its extract was 0.86 ppm, while its nanoparticle has the better antioxidant activity of 0.21 ppm. Conclusion: Nanoparticles of Chromolaena odorata have very strong antioxidant activity and the potential to be external antioxidants.

Preparation and Photocatalytic Properties of Rod-Shaped TiO2 Based on Salix Fiber

2014 ◽  
Vol 609-610 ◽  
pp. 250-254
Author(s):  
Ya Bin Li ◽  
Jin Tian Huang ◽  
Yan Fei Pan
Keyword(s):  
Photocatalytic Performance ◽  
Sol Gel ◽  
Average Diameter ◽  
X Ray Diffraction ◽  
Nanocomposite Structure ◽  
X Ray ◽  
Infrared Spectrometer ◽  
Ft Ir ◽  
Degradation Of Methyl Orange ◽  
Scanning Electron

In the paper, the TiO2nanomaterials adopted the microcrystalline cellulose as the template by the template method and sol-gel method was prepared. Through the infrared spectrometer (FT-IR), scanning electron microscope (SEM), X-ray diffraction (XRD), the surface morphology, composition and the type of the samples were characterized respectively. The influence of the macro morphology of TiO2photocatalytic performance to use the reaction of decolorization and degradation of methyl orange as model was analyzed. The results showed that TiO2which was produced by the template of sallix fiber was Rod-shaped and the average diameter size of nanocomposite structure was 20.592 nm, which can provide a new method of producing other morphology of TiO2.

In-Vitro Antioxidant, Antibacterial and Anticancer properties of Leaf Extract Annona Muricata

2021 ◽  
Vol 8 (1) ◽  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Anticancer Properties

Annona muricata is one of the important herbal plant that are widely used to treat antidiabetic, anti-inflammatory, insecticidal, antimalarial, anticancer, antibacterial and antioxidant activities. In the present study, extraction procedure was carried out in few step processes method. The extract contains high percentage of steroid, alkaloid, flavonoid, phenolic and saponin. The extract was used to study the antioxidant activity; antibacterial activities and anticancer activity by standard methods. The antioxidant activity was studied by using radical scavenging DPPH, FRAP and H2O2 method and disc diffusion method. The results of antibacterial activity of the following bacteria’s such as Pseudomonas, Staphylococcus, Bacillus, E.coli shows the maximum zone of inhibition against Staphylococcus aureus which is around 1.7 cm. The anticancer activity was carried out by MTT assay using Hep-G2 as cell line and results are reported in the paper.

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