21 Comparative Study on Epididymal Spermatozoa Traits of Large White×Landrace and Kolbroek Boars

2018 ◽  
Vol 30 (1) ◽  
pp. 150
Author(s):  
T. R. Netshirovha ◽  
A. T. Kanengoni ◽  
M. B. Matabane ◽  
M. L. Mphaphathi ◽  
A. Maqhashu ◽  
...  
Keyword(s):  
Semen Quality ◽  
Large White ◽  
Sperm Analysis ◽  
Spermatozoa Motility ◽  
Average Percentage ◽  
Semen Volume ◽  
Significant Difference ◽  
Curve Line ◽  
Epididymal Spermatozoa ◽  
The Difference

The Kolbroek pig is an early maturing breed (maturing at 4 to 5 months) that grows more slowly than modern pig breeds. There is general concern that the genetic variation within Kolbroek pig breed is becoming extinct. The aim of the study was to compare semen quality of epididymal spermatozoa derived from slaughtered Large White × Landrace and Kolbroek boars aged between 7 and 9 months. Kolbroek (n = 10) and Large White × Landrace (n = 10) boars were used in this study. Semen was collected from the head of epididymis of Kolbroek and Large White × Landrace testicles. Spermatozoa samples were extracted from head of epididymis by making incision with a razor. Semen samples were then evaluated for macroscopic (semen volume, pH, and concentration) and microscopic characteristics (spermatozoa motility and morphology). Spermatozoa motility was evaluated using computer-aided sperm analysis. Analysis of variance was used to test the difference between the breeds. The average percentage of Kolbroek and Large White × Landrace spermatozoa total motility was 92.4 ± 4.0 and 94.0 ± 4.1%, respectively (P > 0.05). The spermatozoa velocity on the curve line for Kolbroek was lower (147.2 ± 39.2) compared with the Large White × Landrace (178.3 ± 30.1 μm/s; P > 0.05). However, no significant difference was observed between the 2 breeds for rapid spermatozoa and total spermatozoa motility. No significant differences were observed in Kolbroek and Large White × Landrace boar semen volume (8 and 9 mL, respectively) or semen pH (7.0). The average spermatozoa concentration for Kolbroek and Large White × Landrace was 2.5 ± 1.2 and 1.1 ± 1.0 (×109 mL−1), respectively (P > 0.05). There were no significant differences between Kolbroek and Large White × Landrace in abnormal spermatozoa morphology. However, spermatozoa with distal, head, and midpiece abnormalities were significantly different in Kolbroek (14.0 ± 3.6; 3.6 ± 6.0 and 3.4 ± 3.7%) and Large White × Landrace breed (5.4 ± 4.7; 4.7 ± 1.0 and 0.4 ± 1.0%), respectively. In conclusion, macroscopic and microscopic epididymal spermatozoa characteristics of Kolbroek were similar to those of Large White × Landrace boars, except for head and distal spermatozoa abnormalities.

scholarly journals Variance in Stallion Semen Quality among Equestrian Sporting Disciplines and Competition Levels

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 485
Author(s):  
Megan Wilson ◽  
Jess Williams ◽  
V. Tamara Montrose ◽  
Jane Williams
Keyword(s):  
Semen Quality ◽  
Well Being ◽  
Physiological Changes ◽  
Controversial Subject ◽  
Progressive Motility ◽  
Daily Exercise ◽  
Semen Volume ◽  
Significant Difference ◽  
Breeding Programmes ◽  
The Difference

Most stallions within breeding programmes are expected to breed and compete concurrently. The exercising of stallions with regards to training regimes during the breeding season is a controversial subject. Daily exercise at low intensities is important for the mental and reproductive well-being of the stallion, however higher intensities of exercise, as seen in competing stallions, may have detrimental effects on seminal quality. To calculate if competition does affect semen quality, this study investigated the effect that equestrian discipline and timing of competition had on a range of stallion semen characteristics. This was a retrospective study that evaluated the seminal data of 1130 stallion semen collections from two UK based stud farms between 2009 and 2016. Competing stallion semen quality was significantly lower with regards to concentration (p < 0.05) and progressive motility (p < 0.05) than non-competing stallions. Semen volume was higher in competing stallions (p < 0.05) than non-competing stallions. There was a significant difference in seminal attributes among disciplines and competition levels (p < 0.05). The difference in semen quality among competing and non-competing stallions, as well as the difference among disciplines suggests endocrinological and physiological changes occur in relation to training intensity and competition.

165 Comparison of spermatozoa traits and testosterone levels, seminal plasma constituents, and lipid peroxidation of Large White×Landrace and Kolbroek boars

2020 ◽  
Vol 32 (2) ◽  
pp. 209
Author(s):  
T. R. Netshirovha ◽  
R. S. Thomas ◽  
A. T. Kanengoni ◽  
M. B. Matabane ◽  
M. L. Mphaphathi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Seminal Plasma ◽  
Reproductive Traits ◽  
Spectrophotometric Analysis ◽  
Large White ◽  
Testosterone Concentration ◽  
Sperm Analysis ◽  
Spermatozoa Motility ◽  
Sodium Magnesium ◽  
The Difference

Reproductive traits in domestic pigs are one of the most important economic characteristics for improved pig production. Seminal plasma (SP) constituents provide a better indicator of male fertility as measured by semen traits. The objective of this study was to compare spermatozoa traits, testosterone concentration, SP constituents, and lipid peroxidation in Large White×Landrace (LW×LR) and Kolbroek boars aged between 8 and 9 months. Kolbroek (n=12) and LW×LR (n=12) boars were used in this study. Semen was collected using the gloved-hand technique. Blood samples were collected from each boar monthly to determine testosterone concentrations. Spermatozoa motility traits was evaluated using computer-aided sperm analysis. Spermatozoa viability was evaluated using the hyperosmotic swelling test, lipid peroxidation was evaluated using the malondialdehyde (MDA) test, and SP protein was evaluated using atomic absorption spectrophotometric analysis. Analysis of variance was used to test the difference between the breeds. The LW×LR boars had higher ejaculation volume and testosterone concentrations (P&lt;0.05) than Kolbroek boars. The average spermatozoa concentration for Kolbroek and LW×LR boars was 1.8±1.0 and 1.9±1.1×109mL−1, respectively (P&gt;0.05). The average genotype spermatozoa total motility was 91.7±4.0 and 90.6±4.1%, respectively (P&gt;0.05). No significant differences were found between genotype in abnormal spermatozoa morphology, rapid spermatozoa, and total spermatozoa motility, spermatozoa velocity on the curve line, and hyperosmotic swelling test. Spermatozoa with distal, head, and midpiece abnormalities were significantly different in Kolbroek boars (17.1±1.4, 5.0±2.0, and 7.0±2.6%, respectively) and LW×LR boars (9.2±0.1, 3.5±1.1, and 2.6±1.2%, respectively). The LW×LR boars had lower levels of MDA than Kolbroek boars (P&lt;0.05). The SP constituents with selenium, sodium, magnesium, and calcium were significantly different in Kolbroek boars (166.6±1.0, 167.2±0.2, 5.0±0.8, and 10.2±0.3%, respectively) and LW×LR boars (155.0±0.0, 140.0±0.5, 12.0±0.2, and 22.3±0.0%, respectively). In conclusion, spermatozoa parameters of Kolbroek boars were similar to those of LW×LR boars except for semen volume, head and distal spermatozoa abnormalities, testosterone concentration, MDA, and seminal plasma. Further studies need to be conducted on the major seminal plasma constituents as well as their physiological roles.

Analysis of the breeding records of a herd of pigs

1954 ◽  
Vol 45 (1) ◽  
pp. 19-27 ◽  
Author(s):  
R. Braude ◽  
P. M. Clarke ◽  
K. G. Mitchell
Keyword(s):  
Statistical Analysis ◽  
Large White ◽  
Seasonal Differences ◽  
Significant Difference ◽  
The Mean ◽  
The Difference ◽  
Length Of Gestation

1. A survey and. statistical analysis of the records of litters born in the N.I.R.D. herd of Large White pigs over a period of 16 years are presented.2. The mean length of gestation was 114 days for both gilts and sows.3. The mean numbers of pigs born alive and born dead per litter from gilts, 10·0 and 0·2 respectively, were both significantly smaller than the corresponding figures for sows, 11·8 and O·8. No seasonal differences were found.4. The mean total losses of pigs from birth to weaning at 8 weeks were 29·5% of pigs born alive. The losses in litters from gilts, 23·0%, were significantly smaller than in litters from sows, 31·0%. The losses during the winter months both for gilts, 32·5%, and for sows, 35·9%, were significantly higher than during the summer months, 19·4% and 26·7% for gilts and sows respectively.5. Over 50% of the total losses were due to crushing by the dam, 74·1% of the losses from this cause occurring during the first 48 hr. after birth.6. The mean number of pigs weaned per litter was 8·0. The difference between the number weaned per litter from gilts, 7·7, and per litter from number of pigs was weaned per litter from sows during the summer months, 8·7, than during the winter months, 7·4.7. There was no significant difference between the number of male and of female pigs weaned.8. The mean weight at weaning was 29·9 lb., male pigs, 30·3 lb., being significantly heavier than females, 29·51b. Pigs in litters from gilts, 27·81b., were significantly lighter at weaning than those from sows, 30·0 lb., and for both gilts and sows, pigs reared during the summer months, 29·7 and 31·8 lb. respectively, were significantly heavier than those reared during the winter months, 25·9 and 28·2 lb. respectively.

scholarly journals Comparative study of semen quality traits between Etawah grade and Senduro bucks

2021 ◽  
Vol 888 (1) ◽  
pp. 012022
Author(s):  
N Isnaini ◽  
A A Hakim ◽  
D Amertaningtyas ◽  
H E Sulistyo ◽  
A Irsyammawati ◽  
...  
Keyword(s):  
Comparative Study ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
T Test ◽  
Quality Traits ◽  
Spermatozoa Motility ◽  
Frozen Semen ◽  
Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

scholarly journals How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 414 ◽  
Author(s):  
Jiří Šichtař ◽  
Filipa Bubeníčková ◽  
Jitka Sirohi ◽  
Ondřej Šimoník
Keyword(s):  
Plasma Membrane ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Control Group ◽  
Computer Assisted ◽  
Kinematic Parameters ◽  
Prolonged Incubation ◽  
Sperm Analysis ◽  
Spermatozoa Motility

The aim of this study was to evaluate the effect of the addition of two types of seminal plasma (SP) after thawing on the functional characteristics of frozen–thawed (F–T) spermatozoa of poor freezing stallions during prolonged incubation periods. Seminal plasma from stallions with 35–40% (standard seminal plasma, (S-SP)) and 60–70% (above standard seminal plasma, (A-SP)) progressively motile spermatozoa after thawing was used. The motility, kinematic parameters (Computer Assisted Sperm Analysis), distribution of spermatozoa into subpopulations, integrity (carboxyfluorescein diacetate/propidium iodide staining), and functionality (hypo-osmotic swelling (HOS) test) of the spermatozoa plasma membrane were evaluated after thawing (T0) and after 30 min (T30) of incubation at 37 °C. There was no effect of SP addition on spermatozoa motility, but there was a significant positive effect on the kinematic parameters at T0 and T30. The addition of SP significantly increased the percentage of spermatozoa in the fast subpopulation at T0 as well as at T30. Plasma membrane integrity was not affected by the treatment, but functionality significantly decreased by 5% compared to the control group when samples were incubated for 30 min with A-SP. In conclusion, generally, the post-thaw addition of seminal plasma positively affected the post-thaw quality of semen from poor freezing stallions.

scholarly journals Analysis of Screening Results of 1012 Sperm Donors in China: A Retrospective Cohort Study of Geographic Differences in Semen Quality

2021 ◽  
Author(s):  
Qian-Cheng Zhao ◽  
Zhe Zhang ◽  
Lian-Ming Zhao ◽  
Zhen-Feng Deng ◽  
Jiang-Man Gao ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Strong Relationship ◽  
Male Reproduction ◽  
Geographical Differences ◽  
Sperm Donors ◽  
The North ◽  
Significant Difference ◽  
The Difference ◽  
North And South

Abstract Male reproductive health has become a concern in public health, and semen quality is essential to male reproduction. To investigate the geographical differences in semen quality of sperm donors from the north and south of China, a total of 1012 sperm donors from all over China were enrolled in this work, which were divided by their residential latitude. There were statistically significant differences in sperm concentration among men from different latitudes in China(P=0.04). The sperm concentrations of people from 18°-27° north latitude were lower than those from 36°-45°, and 45°-54° (median 131, 134, 146, respectively, P=0.021[18°-27° vs. 36°-45°] and P=0.01[18°-27° vs. 45°-54°]). It was further confirmed when the samples were re-divided into 2 groups (typically north and south) that contains 667 samples. The analysis also showed a significant difference in terms of the regions to which the samples belonged (the median of the north is 134; the median of the south is 125; P =0.015). Although other sperm parameters don’t show significant change with latitude, some of them possess a strong relationship with sperm concentration (r=-0.19364, P<0.001). Specifically, we suppose that environmental pollution and mental stress due to increased population may be the main factors in the difference.

scholarly journals INFLUENCE OF BOOR GENOTYPE ON HEMATOLOGICAL AND BIOCHEMICAL INDICATORS OF BLOOD IN HYBRID PIGS

2021 ◽  
Vol 61 ◽  
pp. 186-191
Author(s):  
І. F. Chernev
Keyword(s):  
Growth And Development ◽  
Calcium Level ◽  
Hemoglobin Content ◽  
Large White ◽  
Group Iii ◽  
Young Pigs ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
The Difference

Purpose of research: In the process of growth and development of pigs, to study the hematological and biochemical parameters of the blood of young pigs obtained by combining two breed sows with purebred and hybrid boars. Material and research methods. The studies were carried out at the State Agrarian University of Moldova and at the pork production enterprise "SC Agroseminvest SRL". To achieve the goal, the research material was two breed sows large white x Landrace (maternal form) and purebred and hybrid boars: I-pietrain; II-large white x landrace x pietrain; III-(large white x landrace x pietrain) x pietrain; IV-duroc; V-pietrain x duroc, (paternal form). In order to determine the influence of hybrid boars on the growth and development of the offspring, five experimental groups were formed according to the principle of analogs of 6 sows and 30 heads of young animals (15 pigs and 15 castrates). Research results .The data obtained indicate an insignificant increase in the hemoglobin content in group I in comparison with group V animals with a moderate significance of the difference, which was 4 g/l (B ≥ 0.95). Consequently, in groups where meat breeds Pietrain and Duroc were used in various combinations, the hemoglobin content was higher. The best results in terms of the content of erythrocytes were obtained in groups III and V, respectively 7.61 x 1012 и and 7.59 x 1012, and a significant difference was established between groups III and IV (B ≥ 0.99), while in the second group the content of erythrocytes was 0.39 lower than in group III (7.22 x 10¹²). Reliable data on the ALT content were obtained between young pigs of groups I and V (B ≥ 0.95). Some tendencies towards a higher content of AST in the second group have been established. The blood glucose level in hybrid young animals in different combination combinations ranged from 5.68 mmol/l (group I) to 6.19 (group II), and the calcium level from 2.55 mmol/l (group III) to 2.81 mmol/l (group II) with a moderate tendency to increase these indicators in group II. Conclusions: A higher hemoglobin content was found in the I group of animals in comparison with the V group with a moderate significance of the difference, which was 4 g/l (B ≥ 0.95). A significant difference was established between groups III and IV (B ≥ 0.99). A higher content of protein in the blood is found in groups I–II, and is more than 89 g/l. In hybrid young animals, the glucose level in different combination combinations ranged from 5.68 mmol/l (group I) to 6.19 (group II), and the calcium level from 2.55 mmol/l (group III) to 2.81 mmol/l (Group II) with a moderate tendency to an increase in these indicators in group II.

scholarly journals KUALITAS SPERMATOZOA AYAM PERANAKAN SENTUL DALAM PENGENCER RINGER LAKTAT KUNING TELUR DENGAN BERBAGAI MONOSAKARIDA (Quality of Sentul Crossbreed Chicken Spermatozoa in Ringer Lactate-Egg Yolk Diluents Supplemented with Various Monosaccharide)

2016 ◽  
Vol 10 (2) ◽  
pp. 166-169
Author(s):  
Khaeruddin Khaeruddin ◽  
Raden Iis Arifiantini ◽  
Cece Sumantri ◽  
Sri Darwati
Keyword(s):  
Energy Source ◽  
Semen Quality ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Ringer Lactate ◽  
Spermatozoa Motility ◽  
Significant Difference

The aim of this study was to examine the preservation of sentul crossbreed chicken semen in ringer lactate egg yolk diluent supplemented with various monosaccharide. Semen was collected from three roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen with more than 70% motility was divided into four tubes. Each of them diluted with ringer lactate egg yolk glucose (RLEYG), ringer lactate egg yolk fructose (RLEYF), ringer lactate egg yolk xylose (RLEYX) and ringer lactate egg yolk mannose (RLEYM). Semen was stored in refrigerator (5o C) for sixty hours and evaluated every twelve hours for spermatozoa motility and viability. Results showed that no significant difference (P>0.05) among diluents used on spermatozoa quality parameters after dilution and during preservation. Semen quality decrease during storage and at sixty hours of storage, the motility and viability of spermatozoa ranging from 48.33±2.56 to 55.42±2.26% and 58.59±2.87 to 64.83±2.42%, respectively. This research conclude that glucose, fructose, xylose and mannose can be used as energy source for roosters semen during preservation.

scholarly journals Analisis Motilitas Spermatozoa Sapi Aceh Setelah Pembekuan dalam Berbagai Konsentrasi Andromed®

2017 ◽  
Vol 17 (2) ◽  
pp. 112-120 ◽  
Author(s):  
Mukhlis Mukhlis ◽  
Dasrul Dasrul ◽  
Sugito Sugito
Keyword(s):  
Analysis Of Variance ◽  
Freezing Process ◽  
Spermatozoa Motility ◽  
Average Percentage ◽  
Treatment Groups ◽  
Group 4 ◽  
Randomized Design ◽  
Significant Difference ◽  
Group 3

ABSTRAK. Penelitian bertujuan mengetahui pengaruh konsentrasi pengencer AdroMed® terhadap kualitas semen sapi aceh setelah proses pembekuan. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan pengenceran AndroMed®. Kelompok A1: AndroMed® 10% (5 ml AndroMed® + 45 ml Aquadestilata), A2: AndroMed® 15% (7,5 ml AndroMed® + 42,5 ml Aquadestilata), A3: AndroMed® 20%(10 ml AndroMed® + 40 ml Aquadestilata) dan A4: AndroMed® 25% (12,5 ml AndroMed® + 37,5 ml Aquadestilata). Masing-masing kelompok diulangi sebanyak 6 kali. Variabel yang di amati pada penelitian ini adalah Motilitas spermatozoa yang diamati tiap kelompok setelah pembekuan yang selanjutnya dianalisis dengan analysis of variance (ANOVA) pola satu arah yang dilanjutkan dengan uji Duncant. Rata-rata persentase motilitas spermatozoa pada kelompok A1, A2, A3 dan A4 secara berturut-turut adalah 30,69±3,68%, 39,79±2,44%, 45,22±3,17% dan 42,42±4,24%. Hasil penelitian menunjukkan bahwa konsentrasi pengencer AndroMed® berpengaruh secara nyata (P0,05) terhadap persentase motilitas spermatozoa sapi aceh. Persentase motilitas spermatozoa pada kelompok A1 tidak berbeda secara nyata dengan A2, dan keduanya berbeda secara nyata (P0,05) dibandingkan dengan kelompok A3dan A4. Konsentrasi AndroMed® 20% lebih baik dari pada 10%, 15% dan 25% dalam mempertahankan motilitas spermatozoa sapi aceh setelah pembekuan. (Analysis of Aceh Cattle spermatozoa motility after freezing using Andromed® with different concentration)ABSTRACT. The study aims to determine the effect of diluent concentration of AndroMed® against the motility of aceh cattle spermatozoa following freezing process. This study uses a completely randomized design (CRD) with 4 treatment groups. Group 1 used diluent 15%; AndroMed®, Grroup 2 diluent 15%; Group 3 with AndroMed® 20%; and, Group 4 with AndroMed® 25%. Each group was repeated 6 times. Motility of spermatozoa assessed which each group observed after freezing.The motility data obtained were analyzed by one way analysis of variance (ANOVA) followed by Duncant test. The average percentage of motility after freezing were found in the group A1, A2, A3 and A4 respectively are 30,69 ± 3,68%, 39,79± 2,44%, 45,22± 3,17%, and 42,42± 4,24%. Statistical analysis showed that the concentration of diluent AndroMed® significantly affected (P0.05) the motility of aceh cattle spermatozoa. There is no significant difference of sperm motility percentage, between A2 with A1 treatment but  both are significantly different (P0.05) compared to group A3. AndroMed® concentration affects the motility of aceh cattle spermatozoa after freezing. The treatment of 20% AndroMed® concentration were improved the quality of aceh cattle spermatozoa following freezing compared to those with AndroMed® 10%, 15%, and 25%.

scholarly journals Karakteristik Semen Ayam IPB-D1

2019 ◽  
Vol 7 (2) ◽  
pp. 57-61
Author(s):  
D. R. Setiadi ◽  
H. Hasibuan ◽  
R. Indriastuti ◽  
A. A. Arif ◽  
Z. N. A. Rosyada ◽  
...  
Keyword(s):  
Body Weight ◽  
Semen Quality ◽  
Mass Movements ◽  
Nacl Solution ◽  
Spermatozoa Motility ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Evaluation Parameters

The aim of this study was to examine semen characteristics of IPB-D1 roosters. A total of 15 IPB-D1 roosters were used in this study, healthy, aged between 6 to 9 months, body weight between 1.2-2.0 kg and placed in individual cages 50 cm × 50 cm × 90 cm. Semen were collected using massage techniques on the back and cloaca rooster. Before collection, cloaca is cleaned using tissue that has been moistened with physiological NaCl solution. The evaluation parameters of semen are macroscopically including volume, color, consistency and pH and microscopic include mass movements, spermatozoa motility (%), spermatozoa morphology (%), concentration and number of spermatozoa per ejaculate. The results showed the semen volume of IPB-D1 was 0.10 ± 0.07 mL, milky white in colour, moderate and dense in concistency and pH 6.97 ± 0.27, Microscopic characteristics of IPB-D1 roosters showed mass spermatozoa movement of 2.73 ± 0.46, spermatozoa motility and concentration were 75.00 ± 6.27% and 3257.50 ± 1303.141×106/mL respectively. It was concluded that IPB-D1 roosters produced good semen quality.

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