scholarly journals The effect of undernutrition in the early postnatal period on skeletal muscle tissue

1993 ◽  
Vol 69 (1) ◽  
pp. 141-150 ◽  
Author(s):  
Stephanie S. Ward ◽  
Neil C. Stickland
Keyword(s):  
Biceps Brachii ◽  
Postnatal Period ◽  
Muscle Fibre Types ◽  
Control Group ◽  
Total Protein Content ◽  
Muscle Fibres ◽  
Relative Growth ◽  
Fibre Area ◽  
Cross Fostering ◽  
Rna Concentration

Guinea-pigs were undernourished from birth by first, cross-fostering in groups of six (in pairs for control animals) and then by feeding a diet of 60% ofad lib. intake from 2 to 6 weeks. The changing characteristics of muscle fibre types in thebiceps brachiiand soleus muscles were monitored by histochemistry. Undernutrition appeared to delay maturation of muscle fibres. Fibre cross-sectioned area was reduced in all fibres of the 60% ofad lib.intake group. Fibres of thebiceps brachiiwere more affected than those of thesoleus. Fibre area vulnerability was partly associated with a high relative growth rate inbiceps brachiiat this time. Total protein content in thesemitendinosusmuscle was depressed in the undernourished group compared with the control group. DNA concentrations were initially higher in the control group but decreased in both groups to similar levels by 6 weeks. RNA concentration increased up to 5 weeks in the control group and then decreased, but decreased after 2 weeks in the undernourished group.

scholarly journals Effect of synbiotic dietary supplementation on histological and histopathological parameters of Pectoralis major muscle of broiler chickens

2018 ◽  
Vol 63 (No. 7) ◽  
pp. 263-271 ◽  
Author(s):  
J. Bogucka ◽  
D.M. Ribeiro ◽  
R.P.R. Costa ◽  
M. Bednarczyk
Keyword(s):  
Muscle Fibre ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Fibre Diameter ◽  
Pectoralis Major ◽  
Muscle Fibre Types ◽  
Control Group ◽  
Muscle Fibres ◽  
Histopathological Changes ◽  
The Impact

Bioactive substances may constitute an alternative to antibiotics. Probiotics are mixtures of microorganisms that enhance the effectiveness and yield of nutrient absorption by competing for the substrate against pathogens that may cause intestinal infections. Prebiotics are organic substances which include complex carbohydrates and have an influence on the growth and activity of desirable intestinal microflora. Synbiotics are a combination of both of them. The aim of this study was to analyze the impact of synbiotics added to commercial feed on Pectoralis major muscle microstructure in broiler chickens. Research material consisted of 240 one-day-old Ross 308 female chicks. Birds were divided into 2 groups, 12 broilers each (replicated 10 times). The control group (C) was fed a commercial diet, and the SYN group was fed the same diet with added synbiotic: 0.8% of prebiotic RFO (raffinose family oligosaccharides) extracted from lupin seeds and 1% of probiotic Lavipan<sup>®</sup>. Birds were slaughtered at day 42. Immediately after slaughter, samples of the Pectoralis major muscle for histological analysis were taken and frozen in liquid nitrogen. The staining procedures performed were: hematoxylin and eosin staining to evaluate fibre diameter and histopathological changes, oil red staining to determine intramuscular fat content, NADH-TR (tetrazolium reductase) activity to distinguish muscle fibre types and alkaline phosphatase reaction for counting the number of capillaries. The tendency to reduced muscle fibre diameter in chickens supplemented with synbiotics indicates an increase in muscle fibre density, which gives meat a more delicate structure. When assessing the occurrence of histopathological changes, significantly more fibre splitting was observed in the control group. Moreover, the positive effect of feed supplementation with synbiotics on muscle microvascularization could have an important practical meaning, because low physical activity of chickens during rearing may lead to ischemic muscles, increased changes in the structure of muscle fibres, and reduction of meat quality.

The effect of ethanol on some hematological parameters of the australian red claw crayfish (Cherax quadricarinatus)

2022 ◽  
pp. 85-91
Author(s):  
D. Skafar ◽  
D. Shumeyko
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Hematological Parameters ◽  
Group Versus ◽  
Control Group ◽  
Total Protein Content ◽  
Cherax Quadricarinatus ◽  
Total Hemocyte Count ◽  
Red Claw Crayfish ◽  
Experimental Group

Purpose: to study the effect of ethanol on the parameters of THC, the percentage of granulocytes and total protein in the hemolymph of the Red claw crayfish (Cherax quadricarinatus).Materials and methods. The object of this experiment was 26 males of the Australian red-clawed crayfish (Cherax quadricarinatus) weighing from 23 to 83 g. The individuals were evenly divided into two experimental groups - with an injection of ethanol and a control group without an injection of 13 crayfish for each group. The injection dose was 2515 mg per 100 g of body weight. A day after the introduction of ethanol, hemolymph was taken with a syringe from the ventral sinus, the syringe was pre-washed with a 4% EDTA-Na2 solution. Three parameters were determined: the total hemocyte count (THC), percent granulocytes and percent total protein content. Counting of hemocytes and determination of granulocytes were performed in a Goryaev chamber under a light microscope. The total protein was determined by the refractometric method.Results. Differences in THC and total protein between the groups were statistically unreliable (p>0,05). THC in the experimental group is 36% more than in the control group. The total protein after the introduction of ethanol actually increased by 0,7%, and relatively by 14%. There were statistically different indicators of the proportion of granulocytes (p<0,05) - the average value of 33,1% in the experimental group versus 24,5% in the control group. A reliable (p<0,05) strong feedback was revealed between the total protein and the mass of individuals in both experimental groups, while in the experimental group there is a visible shift in the values of dependent hemolymph indicators towards an increase in smaller individuals.Conclusion. A single injection of ethyl alcohol with a dosage of 2515 mg per 100 g of body weight into the hemolymph of C. quadricarinatus does not cause significant changes in the THC and total protein after 24 hours. At the same time, the proportion of granulocytes actually increases by 9%, relative to 37%. This may indicate that granulocytes are involved in the formation of cancer defense mechanisms when exposed to toxic substances. The effect of different dosages of ethanol injections and the duration of its effect on hematological parameters requires additional consideration. It is necessary to investigate its effect on other indicators, such as the pH and buffer capacity of the hemolymph, the concentration of hemocyanin, glucose, lactates and calcium.

scholarly journals Effect of Methanolic Extract of Caryota no on Antioxidant Levels in Drosophila melanogaster

2020 ◽  
pp. 27-35
Author(s):  
Chinonye A. Maduagwuna ◽  
Simeon Omale ◽  
Monday A. Etuh ◽  
Steven S. Gyang
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Methanolic Extract ◽  
Control Group ◽  
Total Protein Content ◽  
Total Thiol ◽  
Methanolic Extracts ◽  
Centre Of Excellence ◽  
Anti Oxidant ◽  
Plateau State

Aims: To investigate the anti-oxidant activity of the methanolic extracts of Caryota no seeds in Drosophila melanogaster (DM). Study Design: Experimental design. Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019.                                                                                                                                                     Methodology: These assays were conducted by exposing 50 flies per vial to the selected concentrations (350 mg, 400 mg and 500 mg) of the extract in 5 independent replicates for seven days while control group received distilled water. The total protein content was then determined from the supernatant of the fly homogenate. The antioxidant activity and levels of GST, CAT and total thiol were then measured. The statistical difference among test groups was presumed at P < .05.                                                                                                         Results: The methanolic extract of Caryota no caused nonsignificant (P = .33) decrease in total proteins in DM below basal levels in a dose-dependent pattern.  The antioxidant activity showed nonsignificant (P = .28) lowering of the GST activity in DM below control levels. The methanolic extract of CN nonsignificantly (P > .05) increased the levels of catalase (P = .36) and total thiol levels (P = .22).                                                                                                       Conclusion: It can therefore be concluded that the methanolic extract of Caryota no contains appreciable concentrations of different types of antioxidants. This may provide perspectives for the evaluation and development of effective and safe phytomedicines created from the local biodiversity. 

scholarly journals Effect of ellagic acid on growth and some antioxidant parameters in scaly carp (Cyprinus carpio)

2021 ◽  
Vol 38 (3) ◽  
pp. 337-343
Author(s):  
Tuncay Eksen ◽  
Serpil Mişe Yonar
Keyword(s):  
Ellagic Acid ◽  
Specific Growth ◽  
Reduced Glutathione ◽  
Live Weight ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Relative Growth ◽  
Carp Cyprinus Carpio ◽  
Antioxidant Parameters ◽  
Liver And Kidney

In the present study, it was investigated the effects of various levels of dietary ellagic acid on growth performance and antioxidant status in scaly carp (Cyprinus carpio). Fish were fed with the control diet and three different experimental diets containing three graded levels of ellagic acid (50, 100 and 200 mg kg-1 diet) for 60 days. On 30th and 60th days of experiment, the growth performance [live weight gain, relative growth and specific growth rate] and oxidant/antioxidant parameters [malondialdehyde level, catalase and glutathione-S-transferase activities and reduced glutathione level] were analysed. There was no statistically significant difference in the live weight gain, relative growth and specific growth rates of the control and ellagic acid treated groups (p > 0.05). When compared to the control group, the liver and kidney malondialdehyde levels of ellagic acid treated groups were significantly decreased (p < 0.05). The liver and kidney catalase and glutathione-S-transferase activities and reduced glutathione levels of ellagic acid treated groups were significantly increased when compared to the control group (p < 0.05). It was concluded that ellagic acid can be used as an antioxidant in fish.

scholarly journals Influence guanidinoacetic acid on the growth of young animals quails meat direction of productivity

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
M. Sychov ◽  
H. Pryumak
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Control Group ◽  
Average Growth ◽  
Absolute Increase ◽  
Relative Growth ◽  
Guanidinoacetic Acid ◽  
Relative Gains ◽  
Daily Gain ◽  
Different Levels

The effect of using different levels guanidinoacetic acid in compound feeds on the growth of young quails meat direction of productivity. It is proved that adding to the basic diet of different levels guanidinoacetic acid (0.06, 0.12, 0.18 %) increases live weight of quails on 3.9 – 13.5 g and improves the absolute, average daily and relative gains compared to a bird in the control group, which were fed the main diet. The data show that at the age of 42 days of live weight was the highest in the diet of quail which was administered 0.12% GAA and dominant control of 13.5 g However, in 21 days live weight of quail fed 0.06 and 0.12 % guanidinoacetic acid was greater than control respectively 9.2 and 7.6 grams (P < 0.01). In absolute increments quail, fed GAA prevailed bird control group. The largest absolute increase was in birds that consumed the main diet of GAA level of 0.12% and prevailed controls 6.5 g (P < 0.001). A similar situation was of average daily gain. Young animals that received feed containing 0.12% guanidinoacetic acid had the highest average growth, which exceeded 0.9 g (15.5%) bird control group. As for the relative performance increases here were different. The lowest relative growth observed in birds which were administered GAA 0.06%, it lagged behind the controls 0.1 g in the same period quail treated with 0.12 and 0, 18% guanidinoacetic acid superior control respectively 2 and 1.1 g.

scholarly journals Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”

2020 ◽  
Vol 3 (2) ◽  
pp. 42-46
Author(s):  
V. G. Stoyanovskyy ◽  
M. O. Shevchuk ◽  
I. A. Kolomiiets ◽  
V. A. Kolotnytskyy
Keyword(s):  
Total Protein ◽  
Protein Metabolism ◽  
Broiler Chickens ◽  
Stress Factor ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Combined Stress ◽  
Stages Of Development ◽  
Γ Globulins

The body of  broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.

scholarly journals Pelvic floor muscle training in radical prostatectomy: a randomized controlled trial of the impacts on pelvic floor muscle function and urinary incontinence

BMC Urology ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Joanne E. Milios ◽  
Timothy R. Ackland ◽  
Daniel J. Green
Keyword(s):  
Radical Prostatectomy ◽  
Pelvic Floor ◽  
Muscle Function ◽  
Pelvic Floor Muscle Training ◽  
Pelvic Floor Muscle ◽  
Intervention Group ◽  
Control Group ◽  
Muscle Fibres ◽  
Muscle Training ◽  
Pelvic Floor Muscle Function

Abstract Background Pelvic floor muscle training (PFM) training for post-prostatectomy incontinence (PPI) is an important rehabilitative approach, but the evidence base is still evolving. We developed a novel PFM training program focussed on activating fast and slow twitch muscle fibres. We hypothesized that this training, which commenced pre-operatively, would improve PFM function and reduce PPI, when compared to a control group. Methods This randomized trial allocated 97 men (63 ± 7y, BMI = 25.4, Gleason 7) undergoing radical prostatectomy (RP) to either a control group (n = 47) performing low-volume rehabilitation, or an intervention group (n = 50). Both interventions commenced 5 weeks prior to surgery and continued for 12 weeks post-RP. Participants were assessed pre-operatively and at 2, 6 and 12 weeks post-RP using 24 h pad weights, International Prostate Symptom Score (IPSS), Expanded Prostate Cancer Index Composite for Clinical Practice (EPIC-CP) and real time ultrasound (RTUS) measurements of PFM function. Results Following RP, participants in the control group demonstrated a slower return to continence and experienced significantly more leakage (p < 0.05), measured by 24 h pad weight, compared to the intervention group, suggesting an impact of the prehabilitation protocol. PFM function measures were enhanced following RP in the intervention group. Secondary measures (IPSS, EPIC-CP and RTUS PFM function tests) demonstrated improvement across all time points, with the intervention group displaying consistently lower “bothersome” scores. Conclusions A pelvic floor muscle exercise program commenced prior to prostate surgery enhanced post-surgical measures of pelvic floor muscle function, reduced PPI and improved QoL outcomes related to incontinence. Trial registration The trial was registered in the Australia New Zealand Clinical Trials Registry and allocated as ACTRN12617001400358. The trial was registered on 4/10/2017 and this was a retrospective registration.

Early Post-Partum Contact and Later Attitudes

1980 ◽  
Vol 3 (3) ◽  
pp. 273-286 ◽  
Author(s):  
Peter de Chateau
Keyword(s):  
Post Partum ◽  
Postnatal Period ◽  
Child Rearing ◽  
Control Group ◽  
Maternal Behaviour ◽  
Relative Importance ◽  
Long Term Effects ◽  
Infant Behaviour

In a randomized, prospective study the long-term effects of early post-partum skin-toskin and suckling contact was studied. In follow-up studies 36 hours, 3 and 12 months after delivery maternal behaviour, infant behaviour, the duration of breast feeding and certain attitudes towards child rearing procedures were shown to develop differently in a group of mothers and infants with early post-natal contacts as compared to a control group. Three years after delivery parents with early contact appreciated their children's language development to be faster; the number of siblings born in these families was greater than in controls. In the discussion, the relative importance of the immediate postnatal period is emphasized, a more family oriented development seems to occur in the presence of early post-delivery interaction.

BIOTECHNOLOGICAL METHODS OF INCREASING THE EFFICIENTY OF CATTLE BREEDING

10.12737/7744 ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. 124-127
Author(s):  
Самоделкин ◽  
Aleksandr Samodelkin ◽  
Еремин ◽  
Sergey Eremin
Keyword(s):  
Weight Gain ◽  
Live Weight ◽  
Reproductive Function ◽  
Gastrointestinal Diseases ◽  
Positive Influence ◽  
Postnatal Period ◽  
Control Group ◽  
Cattle Breeding ◽  
Live Weight Gain ◽  
Newborn Calves

There was studied the distribution of postpartum pathology of cows and disease of calves in early postnatal period in conditions of Nizhny Novgorod region. It was established, that the new tissue drug &#34;bio-tec&#34; has positive influence on reproductive function, safety and live weight gain of calves. The postpartum pathology of cows gained wide spread among nizhnynovgorod farms up to 80%, e.g. Endometritis and subinvolution of uterus 37,4 and 23,9 respectively. By clinical examination of newborn calves it was established, that in the first month of life 50,3% fall ill with gastrointestinal diseases of various etiologies. The die-off of calves reached 29%. Using &#34;bio-tec&#34; drug to dry cows decreased the percentage of postpartum pathology by 35% and reduced the time of involution of uterus for 11,2 days; the service period reduced by 21,2 days and the diseases of newborn calves was twice less, that increased the energy of growth and in 16 month age heifers went over animals of the control group by 10%, that allowed their earlier use for reproduction. So, the results of conducted research showed, that twice a day injection of tissue drug &#34;bio-tec&#34; to dry cows in 10ml dose 60-55 and 40-35 days to calving contributes reducing of obstetric pathology. Subsequent use to calves in 4ml dose in 5-8 days age increases the safety of calves and positively influences the live weight gain, that in fact gives abilities of more effective cattle breeding.

Functional Effects of Inertial Training of the Upper Extremity

2003 ◽  
Vol 12 (3) ◽  
pp. 229-239 ◽  
Author(s):  
Todd A. McLoda ◽  
Kate M. Murphy ◽  
Steve Davison
Keyword(s):  
Upper Extremity ◽  
Outcome Measures ◽  
Repeated Measures ◽  
Biceps Brachii ◽  
Exercise Group ◽  
Emg Activity ◽  
Control Group ◽  
Significant Group ◽  
Time Interaction ◽  
Size Groups

Context:Inertial training of the shoulder.Objective:To determine the differences of functional and EMG measures between a control group and an exercise group of overhead throwers.Design:Repeated measures.Setting:Research laboratory.Participants:17 intercollegiate baseball players and 19 intercollegiate softball players divided into 2 equal-size groups, control and exercise.Main Outcome Measures:Preliminary data were recorded, including throwing velocity, throwing accuracy, and EMG activity of the biceps brachii, upper pectorals, and posterior deltoid. The exercise group completed a 4-week training regimen on the Impulse Inertial Trainer. All participants returned for follow-up measures.Results:No significant group-by-time interaction occurred relative to ball velocity, accuracy, or EMG activity.Conclusion:For experienced throwers, functional measures and muscle activity during throwing were not affected by inertial training of the upper extremity.

Sign in / Sign up

Export Citation Format

Share Document