Bioactivities and HPLC analysis of secondary metabolites of a morphologically identified endophytic Aspergillus fungus isolated from Mangifera indica

Knowledge of medicinal plants is often the only therapeutic resource of many communities and ethnic groups. “Erva-baleeira”,Cordia verbenaceaDC., is one of the species of plants currently exploited for the purpose of producing a phytotherapeutic product extracted from its leaves. In Brazil, its major distribution is in the region of the Atlantic Forest and similar vegetation. The crude extract is utilized in popular cultures in the form of hydroalcoholic, decoctions and infusions, mainly as antimicrobial, antiinflammatory and analgesic agents. The aim of the present study was to establish a chemical and comparative profile of the experimental antibacterial activity and resistance modifying activity with ethnopharmacological reports. Phytochemical prospecting and HPLC analysis of the extract and fractions were in agreement with the literature with regard to the presence of secondary metabolites (tannins and flavonoids). The extract and fraction tested did not show clinically relevant antibacterial activity, but a synergistic effect was observed when combined with antibiotic, potentiating the antibacterial effect of aminoglycosides. We conclude that tests of antibacterial activity and modulating the resistance presented in this work results confirm the ethnobotanical and ethnopharmacological information, serving as a parameter in the search for new alternatives for the treatment of diseases.

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Effect of plant extracts on methanogenesis and microbial profile of the rumen of buffalo: a brief overview

Australian Journal of Experimental Agriculture ◽

10.1071/ea07268 ◽

2008 ◽

Vol 48 (2) ◽

pp. 175 ◽

Cited By ~ 46

Author(s):

D. N. Kamra ◽

A. K. Patra ◽

P. N. Chatterjee ◽

Ravindra Kumar ◽

Neeta Agarwal ◽

...

Keyword(s):

Secondary Metabolites ◽

Essential Oils ◽

Plant Extracts ◽

Mangifera Indica ◽

Plant Secondary Metabolites ◽

Feed Additives ◽

Production Test ◽

Syzygium Aromaticum ◽

Ciliate Protozoa

Plants rich in secondary metabolites (saponins, tannins, essential oils, etc.) have antimicrobial activity which can be exploited for selective inhibition of a particular group of microbes in the rumen. We have screened a large number of plant extracts for their potential to inhibit methanogenesis and ciliate protozoa in an in vitro gas production test using buffalo rumen liquor as the inoculum. Out of 93 plant extracts tested, 11 inhibited in vitro methanogenesis to the extent of 25–50% and nine plant extracts inhibited methanogenesis more than 50%. Among 20 extracts exhibiting antimethanogenic activity, nine were ethanol extracts, 10 were methanol extracts and only one was a water extract. Some of these plant extracts inhibited ciliate protozoa as tested by microscopic examination and 14C-labelled radioisotopic technique, but the protozoa inhibition was not correlated with methane inhibition, indicating that the methanogens sensitive to plant secondary metabolites may or may not be having any symbiotic relationship with ciliate protozoa. Methane inhibition was accompanied by a drastic fall in the number of methanogens as determined by real time PCR. Plants that appeared to have some potential as feed additives to control methanogenesis by the ruminants are: (i) seed pulp of Sapindus mukorossi (rich in saponins) and Terminalia chebula (rich in tannins); (ii) leaves of Populus deltoides, Mangifera indica and Psidium guajava (rich in tannins and essential oils); and (iii) flower buds of Syzygium aromaticum and bulb of Allium sativum (rich in essential oils). Some of the plants reported in literature exhibiting antimethanogenic activity include Equisetum arvense, Lotus corniculatus, Rheum palmatum, Salvia officinalis, Sapindus saponaria, Uncaria gambir and Yucca schidigera.

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An improved method to extract DNA from mango Mangifera indica

Biologia ◽

10.2478/s11756-013-0311-2 ◽

2014 ◽

Vol 69 (2) ◽

Cited By ~ 5

Author(s):

Mohammad Uddin ◽

Wenli Sun ◽

Xinhua He ◽

Jaime Teixeira da Silva ◽

Qi Cheng

Keyword(s):

Secondary Metabolites ◽

Dna Extraction ◽

Genomic Dna ◽

Mangifera Indica ◽

Leaf Tissue ◽

Poor Quality ◽

Ammonium Bromide ◽

High Quality ◽

Woody Perennials ◽

Ctab Method

AbstractHigh quality genomic DNA is the first step in the development of DNA-based markers for fingerprinting and genetic diversity of crops, including mango (Mangifera indica L.), a woody perennial. Poor quality genomic DNA hinders the successful application of analytical DNA-based tools. Standard protocols for DNA extraction are not suitable for mango since the extracted genomic DNA often contains secondary metabolites that interfere with analytical applications. In this study, we employed an additional step to remove polysaccharides, polyphenols and secondary metabolites from genomic DNA extracted from young or mature leaf tissue; then a modified traditional cetyl trimethyl ammonium bromide (CTAB) method was applied. The use of 0.4 M glucose improved DNA quality and avoided contamination and browning by polyphenolics, relative to the traditional CTAB method. This is an easy and efficient method for genomic DNA extraction from both young and mature leaves of mango. The isolated DNA was free of polysaccharides, polyphenols, RNA and other major contaminants, as judged by its clear colour, its viscosity, A260/A280 ratio and suitability for PCR-based reactions. This modified protocol was also used to extract high quality genomic DNA from other woody perennials, including walnut, guava, lychee, pear, grape and sugarcane.

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Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

The EuroBiotech Journal ◽

10.2478/ebtj-2019-0002 ◽

2019 ◽

Vol 3 (1) ◽

pp. 10-18 ◽

Cited By ~ 3

Author(s):

Peter M. Eze ◽

Joy C. Nnanna ◽

Ugochukwu Okezie ◽

Happiness S. Buzugbe ◽

Chika C. Abba ◽

...

Keyword(s):

Acetic Acid ◽

Secondary Metabolites ◽

Endophytic Fungi ◽

Hplc Analysis ◽

Antimicrobial Activities ◽

Industrial Applications ◽

Diffusion Method ◽

Fungal Isolation ◽

Indole 3 Acetic Acid ◽

Fungal Extracts

AbstractEndophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves ofNewbouldia laevis, Ocimum gratissimum, andCarica papayaThe plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two fromN. laevis(NL-L1 and NL-L2), one fromO. gratissimum(SL-L1), and two fromC. papaya(PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only againstS. aureus, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungiC. albicansandA. fumigatusHPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.

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Discovery of novel secondary metabolites from fungi—is it really a random walk through a random forest?

Canadian Journal of Botany ◽

10.1139/b95-340 ◽

1995 ◽

Vol 73 (S1) ◽

pp. 925-931 ◽

Cited By ~ 33

Author(s):

Richard L. Monaghan ◽

Jon D. Polishook ◽

Victor J. Pecore ◽

Gerald F. Bills ◽

Mary Nallin-Omstead ◽

...

Keyword(s):

Random Walk ◽

Random Forest ◽

Secondary Metabolites ◽

Key Words ◽

Hplc Analysis ◽

Morphological Similarity ◽

Fermentation Conditions ◽

Random Events ◽

The Mean ◽

Solid Fermentation

Twenty-nine Nodulisporium strains isolated from material obtained worldwide were found to produce secondary metabolites as measured by HPLC. Analysis of incubation conditions resulted in the clustering of three solid fermentation conditions and the clustering of five liquid fermentation conditions. Coverage of 69% of the products produced under eight fermentation conditions could be accomplished if one medium from each cluster was used. Subdivision of the Nodulisporium strains into groups based upon morphological similarity allows for a minimization of the rediscovery of common metabolites. Rare metabolites (HPLC peaks) appeared to occur as random events. However, within the set of cultures that produced the mean or greater than the mean number of metabolites, were found all of the producers of rarer metabolites. Key words: Nodulisporium, fermentation screening, endophytes.

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Distribution and Taxonomic Significance of Secondary Metabolites Occurring in the Methanol Extracts of the Stonecrops (Sedum L., Crassulaceae) from the Central Balkan Peninsula

Natural Product Communications ◽

10.1177/1934578x1501000637 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000

Author(s):

Gordana S. Stojanović ◽

Snežana Č. Jovanović ◽

Bojan K. Zlatković

Keyword(s):

Cluster Analysis ◽

Chemical Composition ◽

Secondary Metabolites ◽

Hplc Analysis ◽

Methanol Extract ◽

Hierarchical Cluster ◽

Balkan Peninsula ◽

Taxonomic Significance ◽

Methanol Extracts ◽

Agglomerative Hierarchical Cluster Analysis

The present study is engaged in the chemical composition of methanol extracts of Sedum taxa from the central part of the Balkan Peninsula, and representatives from other genera of Crassulaceae ( Crassula, Echeveria and Kalanchoe) considered as out-groups. The chemical composition of extracts was determined by HPLC analysis, according to retention time of standards and characteristic absorption spectra of components. Identified components were considered as original variables with possible chemotaxonomic significance. Relationships of examined plant samples were investigated by agglomerative hierarchical cluster analysis (AHC). The obtained results showed how the distribution of methanol extract components (mostly phenolics) affected grouping of the examined samples. The obtained clustering showed satisfactory grouping of the examined samples, among which some representatives of the Sedum series, Rupestria and Magellensia, are the most remote. The out-group samples were not clearly singled out with regard to Sedum samples as expected; this especially applies to samples of Crassula ovata and Echeveria lilacina, while Kalanchoe daigremontiana was more separated from most of the Sedum samples.

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SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KERNEL BIJI BUAH BACANG (Mangifera foetida L.) TERHADAP Escherichia coli PHYTOCHEMICAL SCREENING AND ANTIBACTERIA ACTIVITIES OF ETHANOLIC EXTRACT OF BACANG (Mangifera foetida L.) SEEDS AGAINST Escherichia coli

Pharma Xplore : Jurnal Ilmiah Farmasi ◽

10.36805/farmasi.v1i2.500 ◽

2016 ◽

Vol 1 (2) ◽

Author(s):

Vera Nurviana ◽

Neni Sri Gunarti

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Mangifera Indica ◽

Ethanolic Extract ◽

Test Tube ◽

Diffusion Method ◽

Phytochemical Screening ◽

Key Word ◽

Escherichia Coli Bacteria

Mangifera foetida L. merupakan kerabat Mangifera indica dari genus Mangifera yang diduga memiliki kandungan matabolit sekunder dan efek farmakologis yang hampir sama. Kernel biji buah Mangifera indica dilaporkan memiliki aktivitas antibakteri terhadap Escherichia coli. Berdasarkan pendekatan taksomi maka penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dan aktivitas antibakteri ekstrak etanol 96% kernel biji buah Mangifera foetida L. terhadap Escherichia coli. Skrining fitokimia dilakukan dengan menggunakan uji tabung. Uji aktivitas antibakteri menggunakan metode difusi agar sumuran. Kandungan metabolit sekunder yang terdapat pada ekstrak etanol 96% kernel biji buah Mangifera foetida L. yaitu flavonoid, tanin, polifenol, monoterpen, seskuiterpen, dan kuinon. Hasil pengujian aktivitas antibakteri ekstrak kernel biji buah Mangifera foetida Lour terhadap bakteri Escherichia coli pada beberapa konsentrasi secara berturut-turut 10%, 20%, 30%, 40%, 50%, 60% 70%, 90% dan 100% menghasilkan diameter secara berturut-turut (10,2, 12,03, 14,55, 16,15, 17,18, 18,60, 19,10, 20,20, 21,12) mm yang ditandai dengan terbentuknya zona bening (bersifat bakterisid). Berdasarkan data tersebut maka Kernel Biji Buah Mangifera foetida dinyatakan memiliki aktivitas antibakteri. Kata Kunci; Skrining Fitokimia, Kernel Biji Buah, Mangifera foetida, Antibakteri, Escherichia coli. ABSTRACT Mangifera foetida and Mangifera indica L. are relatives of the genus Mangifera which allegedly contains matabolit secondary with pharmacological effects are almost the same. Mangifera indica seeds are reported to have antibacterial activity against Escherichia coli. Based on scientific classification approach, the study aims to determine the content of secondary metabolites and the antibacterial activity of extract ethanolic 96% Mangifera foetida L. seeds against Escherichia coli. Phytochemical screening is done by using a test tube method. Antibacterial activity test using agar well diffusion method. The content of secondary metabolites found in Ethanolic extract 96 % of the seed Mangifera foetida L. are flavonoids, tannins, polyphenols, monoterpenes, sesquiterpenes, and Quinones. The Results of testing the extracts for antibacterial activity of Mangifera foetida Lour seeds against Escherichia coli bacteria at some concentrations of respectively 10 % , 20 % , 30 % , 40 % , 50 % , 60 % 70 %, 90 % and 100 % have produced diameter in respectively (10.2, 12.03, 14.55, 16:15, 17:18, 18.60, 19:10, 20:20, 21:12) mm, are Characterized by the formation of a clear zone (bakterisid). Based on of these data, the seed of Mangifera foetida fruits stated to have antibacterial activity. Key Word: Phytochemical Screening, Seed of Mangifera foetida Fruit, Antibacteria, Escherichia coli

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CALLUS AND CELL SUSPENSION CULTURE OF DROSERA BURMANNI VAHL FOR QUINONE PRODUCTION

Science and Technology Development Journal ◽

10.32508/stdj.v13i2.2126 ◽

2010 ◽

Vol 13 (2) ◽

pp. 53-61

Author(s):

Phuong Ngo Diem Quach ◽

Minh Thi Thanh Hoang ◽

Thu Thi Hoang ◽

Le Van Bui

Keyword(s):

Secondary Metabolites ◽

Suspension Culture ◽

Cell Suspension ◽

Callus Culture ◽

Bioactive Compounds ◽

Cell Suspension Culture ◽

Hplc Analysis ◽

Cell Biomass ◽

Culture Process

Drosera burmanni Vahl, one of three Drosera species in Vietnam, has been successfully cultured in vitro. Our previous researchs have shown that extracts of Drosera burmanni Vahl contain bioactive compounds such as naphthoquinone, anthraquinone. To obtain cell biomass as well as increase secondary metabolites, callus and cell suspension culture of Drosera burmanni Vahl become extremely urgent. Therefore, in this study, we focused on building Drosera burmanni Vahl callus and suspension culture process to obtain quinone. Our results show that the most optimized medium for callus culture is Gamborg’s B5, saccharose 20g/l, casein 100 mg/l, PVP 1g/l. To induce callus culture, the best hormone’s concentration is 2,4-D 0,2 mg/l, NAA 0,2 mg/l. Growing callus and increasing cell biomass in suspension culture are the same culture type. The peak of growing phase is on 12th. HPLC analysis showed present of plumbagin, one of quinone bioactive compounds determined in Drosera species, on cultured cell suspension.

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Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

Food & Function ◽

10.1039/d0fo01638a ◽

2020 ◽

Vol 11 (10) ◽

pp. 8547-8559

Author(s):

Hongjing Zhao ◽

Yu Wang ◽

Mengyao Mu ◽

Menghao Guo ◽

Hongxian Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Secondary Metabolites ◽

Human Health ◽

Grass Carp ◽

Aquatic Environment ◽

Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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