scholarly journals Probiotic research as highest antimicrobial Listeria monocytogenes from virgin coconut oil [VCO] Padang West Sumatra

2021 ◽  
Vol 741 (1) ◽  
pp. 012012
Author(s):  
S Syukur ◽  
Syafrizayanti ◽  
M Ismet ◽  
S Zulaiha ◽  
Zulkifli
Keyword(s):  
Listeria Monocytogenes ◽  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
West Sumatra

scholarly journals KARAKTERISASI BAKTERIOSIN PADA BAKTERI ASAM LAKTAT Lactobacillus paracasei DARI VIRGIN COCONUT OIL

2020 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Fauzan Fauzan ◽  
Suryani Suryani
Keyword(s):  
Listeria Monocytogenes ◽  
Coconut Oil ◽  
Lactobacillus Paracasei ◽  
Virgin Coconut Oil ◽  
E Coli ◽  
Sds Page

<p><strong><em>Abstrak</em></strong></p><p><em>Virgin Coconut Oil (VCO), selama  ini  dikenal  banyak sekali kegunaan nya disebabkan  kandungan asam lauratnya yang tinggi. Tetapi ternyata tidak hanya itu melainkan  adanya bakteri asam laktat yang mengandung bakteriosin. Dimana bakteriosin adalah peptide yang mempunyai kemampuan menghambat pertumbuhan bakteri dan jamur yang berisifat patogen, bahkan dapat membunuh virus. Tujuan penelitian ini adalah untuk mengkarakterisasi bakteriosin yang ada pada bakteri asam laktat  <strong>Lactobacillus paracasei</strong> yang terdapat pada lapisan minyak VCO.Adapun penelitian ini dimulai dengan mengisolasi bakteriosin menggunakan media </em><em>MRSB (Merck), pemurnian bakteriosin nya dengan purifikasi parsial</em>  <em>menggunakan  metoda pengendapan  Ammium Sulfat. Karakterisasi dilakukan 4 macam, yaitu Kemampuan tumbuhnya  BAL pada medium dilakukan dengan memvariasikan pH tertentu; Kemampuan bakteriosin dihasilkan pada medium dengan variasi pH tertentu, kemudian menganalisa antimikrobanya;</em> <em>Ketahanan bakteriosin tersebut pada pemanasan dengan variasi temperatur tertentu; Penentuan Berat Molekul dengan SDS-PAGE. Hasil purifikasi parsial yang paling bagus adalah pada</em> <em>pengendapan dengan 40% dan 60% ammonium sulfat . Untuk kemampuan tumbuh BAL, ternyata mampu tumbuh pada pH 2-9, Kemampuan aktivitas mikroba bakteriosin pada pH 2-6 untuk E Coli, pH 2-9 untuk B Sbtillis dan pH 2-10 untuk bakteri uji Listeria monocytogenes dan S aureus.</em>  <em>Bakteriosin ini tahan sampai pemanasan 121 <sup>0</sup>C. Sehingga dapat disimpulkan bakteriosin ini bisa  hidup pada manusia</em></p><p><em> </em></p><p><em> </em></p>

ISOLASI BAKTERI PATTOGEN PADA PASIEN PENDERITA INFEKSI TELINGA Chronic supparative otitis media (OMSK)

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Suryani Suryani
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Otitis Media ◽  
Proteus Mirabilis ◽  
Coconut Oil ◽  
Chronic Suppurative Otitis Media ◽  
Virgin Coconut Oil ◽  
Suppurative Otitis Media ◽  
Ear Infection ◽  
Milk Fermentation

<p> </p><p align="center">Abstrak</p><p>Tujuan penelitian ini adalah mengisolasi bakteri pattogen pada infeksi telinga <em>Chronic suppurative otitis media</em> yang nantinya sebagai tahap awal dari pengembangan analisa antimikroba Bakteri Asam Laktat (BAL) pada proses fermentasi santan kelapa menjadi Virgin Coconut  Oil (VCO), yang diharapkan  mampu  berfungsi sebagai antimikroba/antibakteri dari bakteri pattogen. Menurut Suryani dkk (2014), isolat BAL dari fermentasi santan mampu berfungsi sebagai antibakteri terhadap 5 bakteri uji  (<em>E.coli NBRC14237, Staphylococcus aereus NBRC 13276, Bacillus substilis BTCCB, Salmonella thypii, </em>dan<em> Listeria  monocytogenes</em> ). Untuk mendapatkan informasi tersebut maka di isolasi bakteri yang ada di cairan telinga pasien  penderita infeksi telinga <em>Chronic suppurative otitis media </em>dengan menggunakan  media umum  Blood Agar dengan metoda Pengenceran. Dari penelitian ini didapatkan 42 isolat dengan 4 jenis bakteri pattogen yaitu <em>Pseudomonas aureginosa, Staphilococus aureus, Staphilococus epidermidis, Proteus mirabilis. </em>Pasien OMSK yang diambil sekret nya 60 % berumur diatas 20 tahun dan 40% berumur dibawah 20 tahun yang sama banyak antara perempuan dan Laki-laki .</p><p> </p><p>Kata Kunci:    Isolasi bakteri pattogen sekret pasien OMSK, <em>Chronic suppurative otitis </em></p><p><em>media,</em>BAL( Bakteri  Asam Laktat), Virgin Coconut Oil (VCO),</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p><p align="center">The Isolation of Pathogen Bacteria on The Sufferer of Ear Infection Chronic Supparative Otitis Media</p><p align="center"><sup>1</sup>Suryani, <sup>1</sup>Zulmardi,<sup>2</sup> Abdi Dharma, <sup>2</sup>Yunazar Manjang</p><p align="center"><sup>1</sup>Universitas Muhammadiyah Sumatera Barat</p><p align="center"><sup>2</sup>Universitas Andalas</p><p align="center">E-mail [email protected]</p><p align="center"> </p><p align="center">Absract</p><p>This research is aimed to isolate pathogen bacteria in ear infection Chronic Supparative Otitis Media. This is the first phase in developing analysis Antimicroba Lactic Acid. Bacteria in the process of coconut milk fermentation into Virgin Coconut Oil (VCO). It is expected can be fanctioned as antibacteria of pathogen. According to Suryani et.al (2014), isolate of Antimicroba Lactic Acid of coconut milk fermentation can act as antibacteria against 5 examined bacteria (<em>E.coli NBRC14237, Staphylococcus aereus NBRC 13276, Bacillus substilis BTCCB, Salmonella thypii, </em>dan<em> Listeria  monocytogenes</em>). To achieve it, the ear liquid of sufferer containing bacteria is isolated with Blood Agar media and Dilution method. The result is that there 42 isolate in accordance with 3 kinds pathogen bacteria such as <em>Pseudomonas aureginosa, Staphilococus aureus, Staphilococus epidermidis, Proteus mirabilis</em>. The sufferer who has choosen as participant are 72% coming from the age of above 20 and the rest are under 20 years old. The ratio of sexes are similar between male and female.</p><p>Key Word:    Isolation pattogen bacteria sckret  OMSK, <em>Chronic suppurative otitis </em></p><p><em>media,</em> LAB(Lactat Acid Bacteria), Virgin Coconut Oil (VCO),</p><p> </p><p> </p>

PENGARUH VIRGIN COCONUT OIL (VCO) TERHADAP KARAKTERISTIK DAN UMUR SIMPAN ROTI MANIS

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Aulia Alfi
Keyword(s):  
Moisture Content ◽  
Shelf Life ◽  
Coconut Oil ◽  
Temperature Treatment ◽  
Ash Content ◽  
Virgin Coconut Oil ◽  
Food Ingredients ◽  
Life Analysis ◽  
Antibacterial And Antifungal ◽  
Physical And Chemical

Virgin Coconut Oil (VCO) adalah bahan alami yang memiliki sifat antimikroba (antivirus, antibakteri, dan antijamur). Sehingga VCO dapat memberikan efek pengawet pada bahan makanan, salah satunya adalah roti manis. Penelitian ini dilakukan untuk mengevaluasi pengaruh VCO terhadap karakteristik (fisik dan kimia) dan umur simpan roti manis. Roti manis dianalisis secara fisik (tekstur dan porositas) dan kimia (kadar air, kadar abu, kadar lemak, kadar protein, dan kandungan karbohidrat), dan analisis umur simpan dengan FFA, uji organoleptik dan jamur setiap dua hari selama delapan hari penyimpanan di suhu ruang. Variasi perlakuan roti manis adalah dari rasio konsentrasi VCO: margarin: mentega, K (0%: 8%: 8%); A (4%: 6%: 6%); B (8%: 4%: 4%), C (12%: 2%: 2%); D (16%: 0%: 0%). Hasil penelitian menunjukkan bahwa VCO tidak memiliki pengaruh yang signifikan terhadap karakteristik fisik dan karakteristik kimia roti manis. Namun, VCO berpengaruh signifikan terhadap kadar air roti manis yang dihasilkan, roti manis K memiliki kadar air tertinggi (22,36%) dan berbeda dengan sampel roti manis lainnya. VCO secara efektif menghambat pertumbuhan jamur di roti manis pada konsentrasi 8%, 12%, dan 16%. Roti manis K dan A memiliki masa simpan 4 hari, sedangkan roti manis B, C, dan D memiliki masa simpan 6 hari.Kata kunci: VCO, roti manis, karakteristik, umur simpanABSTRACTVirgin Coconut Oil (VCO) is a natural ingredient that has antimicrobial (antiviral, antibacterial, and antifungal) properties. So that VCO can provide a preservative effect on food ingredients, one of which is sweet bread. This research was conducted to evaluate the effect of VCO on characteristics (physical and chemical) and shelf life of sweet bread. Sweet bread was analyzed physically (texture and porosity) and chemistry (moisture content, ash content, fat content, protein content, and carbohydrate content), and shelf life analysis with FFA, organoleptic and mold tests every two days for eight days of storage at ambient temperature. Treatment variations of sweet breads is from the ratio of the concentration of VCO: margarine: butter, K (0%: 8%: 8%); A (4%: 6%: 6%); B (8%: 4%: 4%), C (12%: 2%: 2%); D (16%: 0%: 0%). The results showed that VCO did not have a significant effect on the physical characteristics and chemical characteristics of sweet bread. However, the VCO has a significant effect on the water content of the sweet bread produced, sweet bread K has the highest moisture content (22,36%) and it is different from other sweet bread samples. VCO effectively inhibits the growth of sweet bread mold at concentrations of 8%, 12%, and 16%. K and A sweet bread has a shelf life of 4 days, while sweet breads B, C, and D have a shelf life of 6 days.Keywords: VCO, sweet bread, characteristics, shelf life

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim
Keyword(s):  
Allergic Rhinitis ◽  
Side Effects ◽  
Coconut Oil ◽  
Test Group ◽  
Nasal Symptom ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

PENGARUH PENGGUNAAN VIRGIN COCONUT OIL (VCO) DALAM RANSUM TERHADAP PERFORMANS TERNAK BABI FASE STARTER

ZOOTEC ◽  
2015 ◽  
Vol 35 (2) ◽  
pp. 86
Author(s):  
Alfred Tamawiwy ◽  
M. Najoan ◽  
J S Mandey ◽  
F. N Sompie
Keyword(s):  
Water Consumption ◽  
Feed Intake ◽  
Control Diet ◽  
Coconut Oil ◽  
Growing Pigs ◽  
Virgin Coconut Oil ◽  
Daily Water ◽  
Pig Performance ◽  
Daily Gain ◽  
Daily Water Consumption

ABSTRACT   EFFECT OF UTILIZATION OF VIRGIN COCONUT OIL (VCO) IN THE DIET ON PIG PERFORMANCE. Utilization of fats and oils in pig diets is of great importance due to their high energy value. VCO is obtained by cold press processing of the kernel from the coconut fruit. Utilization of virgin coconut oil (VCO) in the diets on pig performance.  The present study was designed to elaborate the effect of utilization of VCO in the diets on energy and protein digestibility of growing pigs. The experiment was conducted using 20 castrated male pigs aged 1.5 - 2.0 months weighing 12,0±2,0 kg. The data were analyzed according to the linear model procedure for ANOVA appropriate for Randomized Block Design with 5 treatments and 4 replications. Treatments were formulated as follow: R0 = 100% control diet + 0% VCO; R1 = 99.5% control diet + 1.0% VCO; R2 = 98.0% control diet + 2.0% VCO; R3 = 97.0% control diet + 3.0% VCO; and R4 = 96.0% control diet + 4.0% VCO. Parameters measured were: daily feed intake, daily gain, daily water consumption. The results showed that the utilization of VCO up to 4% in the diets had no significant effect (P > 0.05) on daily feed intake, daily gain, daily water consumption of pigs. It can be concluded that the addition of VCO up to 4.0% in the diets has no significant meaning on pig performance.   Key words: Virgin coconut oil (VCO), Performance, Growing pigs  

In Vitro Anticancer Activity of Virgin Coconut Oil and its Fractions in Liver and Oral Cancer Cells

2020 ◽  
Vol 19 (18) ◽  
pp. 2223-2230 ◽  
Author(s):  
Poonam Verma ◽  
Sanjukta Naik ◽  
Pranati Nanda ◽  
Silvi Banerjee ◽  
Satyanarayan Naik ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Oral Cancer ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Cell Line ◽  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
Anticancer Efficacy ◽  
Oral Cancer Cells

Background: Coconut oil is an edible oil obtained from fresh, mature coconut kernels. Few studies have reported the anticancer role of coconut oil. The fatty acid component of coconut oil directly targets the liver by portal circulation and as chylomicron via lymph. However, the anti-cancer activity of coconut oil against liver cancer cells and oral cancer cells is yet to be tested. The active component of coconut oil, that is responsible for the anticancer activity is not well understood. In this study, three different coconut oils, Virgin Coconut Oil (VCO), Processed Coconut Oil (PCO) and Fractionated Coconut Oil (FCO), were used. Objective: Based on previous studies, it can be hypothesized that fatty acids in coconut oil may have anticancer potential and may trigger cell death in cancer cell lines. Methods: Each cell line was treated with different concentrations of Virgin Coconut Oil (VCO), Processed Coconut Oil (PCO) and Fractionated Coconut Oil (FCO). The treated cells were assayed by MTT after 72 hr of incubation. The fatty acid composition of different coconut oils was analyzed by gas chromatography. Result: Different concentrations of coconut oils were used to treat the cells. Interestingly, the anticancer efficacy of VCO, PCO and FCO was not uniform, rather the efficacy varied from cell line to cell line. Only 20% VCO showed significant anticancer activity in HepG2 cells in comparison to 80% PCO against the KB cell line. Remarkably, 20% of PCO and 5% of FCO showed potential growth inhibition in the KB cell line as compared to 80% PCO in HepG2 cells. Moreover, there was a difference in the efficacy of VCO, PCO and FCO, which might be due to their fatty acid composition. Comparing the anticancer efficacy of VCO, PCO and FCO in this study helped to predict which class of fatty acids and which fatty acid might be associated with the anticancer activity of VCO. Conclusion: This study shows that VCO, PCO and FCO have anticancer efficacy and may be used for the treatment of cancer, especially liver and oral cancer.

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