scholarly journals Antibacterial hydrogel microparticles with drug loading for wound healing

2021 ◽  
Vol 8 (9) ◽  
pp. 095403
Author(s):  
Hongzhen Zhang ◽  
Ronghua Xu ◽  
Zuowen Yin ◽  
Jun Yu ◽  
Ning Liang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Drug Loading ◽  
Hydrogel Microparticles

scholarly journals Development of Spanish Broom and Flax Dressings with Glycyrrhetinic Acid-Loaded Films for Wound Healing: Characterization and Evaluation of Biological Properties

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1192
Author(s):  
Angela Abruzzo ◽  
Concettina Cappadone ◽  
Valentina Sallustio ◽  
Giovanna Picone ◽  
Martina Rossi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Drug Loading ◽  
Healing Process ◽  
In Vitro Release ◽  
Sodium Hyaluronate ◽  
Biological Properties ◽  
Glycyrrhetinic Acid ◽  
Wound Dressings ◽  
Biological Studies

The selection of an appropriate dressing for each type of wound is a very important procedure for a faster and more accurate healing process. So, the aim of this study was to develop innovative Spanish Broom and flax wound dressings, as alternatives to cotton used as control, with polymeric films containing glycyrrhetinic acid (GA) to promote wound-exudate absorption and the healing process. The different wound dressings were prepared by a solvent casting method, and characterized in terms of drug loading, water uptake, and in vitro release. Moreover, biological studies were performed to evaluate their biocompatibility and wound-healing efficacy. Comparing the developed wound dressings, Spanish Broom dressings with GA-loaded sodium hyaluronate film had the best functional properties, in terms of hydration ability and GA release. Moreover, they showed a good biocompatibility, determining a moderate induction of cell proliferation and no cytotoxicity. In addition, the wound-healing test revealed that the Spanish Broom dressings promoted cell migration, further facilitating the closure of the wound.

scholarly journals Development of Cephradine-Loaded Gelatin/Polyvinyl Alcohol Electrospun Nanofibers for Effective Diabetic Wound Healing: In-Vitro and In-Vivo Assessments

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 349
Author(s):  
Anam Razzaq ◽  
Zaheer Ullah Khan ◽  
Aasim Saeed ◽  
Kiramat Ali Shah ◽  
Naveed Ullah Khan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyvinyl Alcohol ◽  
Mtt Assay ◽  
Chronic Wound ◽  
Drug Loading ◽  
Group Identification ◽  
Wound Infections ◽  
Diabetic Wound

Diabetic wound infections caused by conventional antibiotic-resistant Staphylococcus aureus strains are fast emerging, leading to life-threatening situations (e.g., high costs, morbidity, and mortality) associated with delayed healing and chronic inflammation. Electrospinning is one of the most widely used techniques for the fabrication of nanofibers (NFs), induced by a high voltage applied to a drug-loaded polymer solution. Particular attention is given to electrospun NFs for pharmaceutical applications (e.g., original drug delivery systems) and tissue regeneration (e.g., as tissue scaffolds). However, there is a paucity of reports related to their application in diabetic wound infections. Therefore, we prepared eco-friendly, biodegradable, low-immunogenic, and biocompatible gelatin (GEL)/polyvinyl alcohol (PVA) electrospun NFs (BNFs), in which we loaded the broad-spectrum antibiotic cephradine (Ceph). The resulting drug-loaded NFs (LNFs) were characterized physically using ultraviolet-visible (UV-Vis) spectrophotometry (for drug loading capacity (LC), drug encapsulation efficiency (EE), and drug release kinetics determination), thermogravimetric analysis (TGA) (for thermostability evaluation), scanning electron microscopy (SEM) (for surface morphology analysis), and Fourier-transform infrared spectroscopy (FTIR) (for functional group identification). LNFs were further characterized biologically by in-vitro assessment of their potency against S. aureus clinical strains (N = 16) using the Kirby–Bauer test and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, by ex-vivo assessment to evaluate their cytotoxicity against primary human epidermal keratinocytes using MTT assay, and by in-vivo assessment to estimate their diabetic chronic wound-healing efficiency using NcZ10 diabetic/obese mice (N = 18). Thin and uniform NFs with a smooth surface and standard size (<400 nm) were observed by SEM at the optimized 5:5 (GEL:PVA) volumetric ratio. FTIR analyses confirmed the drug loading into BNFs. Compared to free Ceph, LNFs were significantly more thermostable and exhibited sustained/controlled Ceph release. LNFs also exerted a significantly stronger antibacterial activity both in-vitro and in-vivo. LNFs were significantly safer and more efficient for bacterial clearance-induced faster chronic wound healing. LNF-based therapy could be employed as a valuable dressing material to heal S. aureus-induced chronic wounds in diabetic subjects.

scholarly journals Affinity Effects on the Release of Non-Conventional Antifibrotics from Polymer Depots

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 275 ◽  
Author(s):  
Nathan A. Rohner ◽  
Dung Nguyen ◽  
Horst A. von Recum
Keyword(s):  
Wound Healing ◽  
Aluminum Toxicity ◽  
Drug Loading ◽  
Anthelmintic Activity ◽  
Bactericidal Effect ◽  
Iron Aluminum ◽  
Deferoxamine Mesylate ◽  
Drug Choice ◽  
Approved Drugs

For many chronic fibrotic conditions, there is a need for local, sustained antifibrotic drug delivery. A recent trend in the pharmaceutical industry is the repurposing of approved drugs. This paper investigates drugs that are classically used for anthelmintic activity (pyrvinium pamoate (PYR)), inhibition of adrenal steroidgenesis (metyrapone (MTP)), bactericidal effect (rifampicin (RIF), and treating iron/aluminum toxicity (deferoxamine mesylate (DFOA)), but are also under investigation for their potential positive effect in wound healing. In this role, they have not previously been tested in a localized delivery system suitable for obtaining the release for the weeks-to-months timecourse needed for wound resolution. Herein, two cyclodextrin-based polymer systems, disks and microparticles, are demonstrated to provide the long-term release of all four tested non-conventional wound-healing drugs for up to 30 days. Higher drug affinity binding, as determined from PyRx binding simulations and surface plasmon resonance in vitro, corresponded with extended release amounts, while drug molecular weight and solubility correlated with the improved drug loading efficiency of cyclodextrin polymers. These results, combined, demonstrate that leveraging affinity interactions, in combination with drug choice, can extend the sustained release of drugs with an alternative, complimentary action to resolve wound-healing and reduce fibrotic processes.

scholarly journals Polymer-Based Materials Loaded with Curcumin for Wound Healing Applications

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2286 ◽  
Author(s):  
Sibusiso Alven ◽  
Xhamla Nqoro ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Wound Healing ◽  
Transmission Rate ◽  
Biological Activities ◽  
Drug Loading ◽  
Healing Process ◽  
Antioxidant Properties ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Microbial Invasion ◽  
Good Biocompatibility

Some of the currently used wound dressings have interesting features such as excellent porosity, good water-absorbing capacity, moderate water vapor transmission rate, high drug loading efficiency, and good capability to provide a moist environment, but they are limited in terms of antimicrobial properties. Their inability to protect the wound from microbial invasion results in wound exposure to microbial infections, resulting in a delayed wound healing process. Furthermore, some wound dressings are loaded with synthetic antibiotics that can cause adverse side effects on the patients. Natural-based compounds exhibit unique features such as good biocompatibility, reduced toxicity, etc. Curcumin, one such natural-based compound, has demonstrated several biological activities such as anticancer, antibacterial and antioxidant properties. Its good antibacterial and antioxidant activity make it beneficial for the treatment of wounds. Several researchers have developed different types of polymer-based wound dressings which were loaded with curcumin. These wound dressings displayed excellent features such as good biocompatibility, induction of skin regeneration, accelerated wound healing processes and excellent antioxidant and antibacterial activity. This review will be focused on the in vitro and in vivo therapeutic outcomes of wound dressings loaded with curcumin.

scholarly journals Extended Release of Timolol from Ethyl Cellulose Microparticles Laden Hydrogel Contact Lenses

2015 ◽  
Vol 2 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Furqan A. Maulvi ◽  
Tejal G. Soni ◽  
Dinesh O. Shah
Keyword(s):  
Physical Properties ◽  
Ethyl Cellulose ◽  
Contact Lenses ◽  
Drug Loading ◽  
Amorphous State ◽  
Eye Drops ◽  
Hydrogel Microparticles ◽  
Zero Order Kinetics ◽  
The Impact

Glaucoma is a second leading cause of blindness globally after cataract, which is managed through eye drops, which are highly inefficient due to a low bioavailability of less than 1-5%. Frequent administration of eye drops leads to incompliance in patients, so there is a great need for medical device such as contact lenses to treat glaucoma. The objective of research was to provide sustained ocular delivery of timolol via prototype poly (hydroxyethyl methacrylate) hydrogel contact lenses which may improve bioavailability due to increase in ocular residence time of drug. The present work was to encapsulate drug in ethylcellulose microparticles, and to entrap these microparticles in the hydrogel. Microparticles were prepared by spray drying method using different ratios of drug to ethylcellulose. The solid state characterization studies of drug loaded microparticles revealed the transformation of drug to an amorphous state. The hydrogels were characterized by studying their optical and physical properties to determine their suitability as extended wear contact lenses. Microparticles laden hydrogels were compared with direct drug loaded hydrogels. The study of microparticles laden hydrogels showed reduction in optical and physical properties and the impact was proportional to the amount of microparticles in hydrogels. The results suggest the application of optimization and nanotechnology. In vitro drug release study revealed that direct loading batch delivers drug for 22 hours with high drug loading of 150 µg, while microparticles laden hydrogel deliver drug up to 48 hours (zero order kinetics) with low drug loading of 50 µg. The hydrogels appeared safe in the cytotoxicity study. The study demonstrated the promising potential of loading the ethyl cellulose microparticles into hydrogels to serve as a good platform for sustained ophthalmic drug delivery.

scholarly journals Performance of Polydopamine Complex and Mechanisms in Wound Healing

2021 ◽  
Vol 22 (19) ◽  
pp. 10563
Author(s):  
Dantong Zheng ◽  
Chongxing Huang ◽  
Xuhao Zhu ◽  
Haohe Huang ◽  
Chenglong Xu
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Photothermal Therapy ◽  
Drug Loading ◽  
Reactive Oxygen ◽  
Wound Dressings ◽  
Inflammatory Factors ◽  
Oxygen Species ◽  
Self Healing

Polydopamine (PDA) has been gradually applied in wound healing of various types in the last three years. Due to its rich phenol groups and unique structure, it can be combined with a variety of materials to form wound dressings that can be used for chronic infection, tissue repair in vivo and serious wound healing. PDA complex has excellent mechanical properties and self-healing properties, and it is a stable material that can be used for a long period of time. Unlike other dressings, PDA complexes can achieve both photothermal therapy and electro activity. In this paper, wound healing is divided into four stages: antibacterial, anti-inflammatory, cell adhesion and proliferation, and re-epithelialization. Photothermal therapy can improve the bacteriostatic rate and remove reactive oxygen species to inhibit inflammation. Electrical signals can stimulate cell proliferation and directional migration. With low reactive oxygen species (ROS) levels, inflammatory factors are down-regulated and growth factors are up-regulated, forming regular collagen fibers and accelerating wound healing. Finally, five potential development directions are proposed, including increasing drug loading capacity, optimization of drug delivery platforms, improvement of photothermal conversion efficiency, intelligent electroactive materials and combined 3D printing.

scholarly journals In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing

Pharmaceutics ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 180 ◽  
Author(s):  
Charu Dwivedi ◽  
Himanshu Pandey ◽  
Avinash C. Pandey ◽  
Sandip Patil ◽  
Pramod W. Ramteke ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Drug Loading ◽  
Physicochemical Characterization ◽  
Diabetic Wound ◽  
Scanning Calorimetry ◽  
Rapid Healing ◽  
Diabetic Wound Healing

Tissue engineering technologies involving growth factors have produced one of the most advanced generations of diabetic wound healing solutions. Using this approach, a nanocomposite carrier was designed using Poly(d,l-lactide-co-glycolide) (PLGA)/Gelatin polymer solutions for the simultaneous release of recombinant human epidermal growth factor (rhEGF) and gentamicin sulfate at the wound site to hasten the process of diabetic wound healing and inactivation of bacterial growth. The physicochemical characterization of the fabricated scaffolds was carried out using scanning electron microscopy (SEM) and X-ay diffraction (XRD). The scaffolds were analyzed for thermal stability using thermogravimetric analysis and differential scanning calorimetry. The porosity, biodegradability, and swelling behavior of the scaffolds was also evaluated. Encapsulation efficiency, drug loading capacity, and in vitro drug release were also investigated. Further, the bacterial inhibition percentage and detailed in vivo biocompatibility for wound healing efficiency was performed on diabetic C57BL6 mice with dorsal wounds. The scaffolds exhibited excellent wound healing and continuous proliferation of cells for 12 days. These results support the applicability of such systems in rapid healing of diabetic wounds and ulcers.

scholarly journals Near-Infrared, Light-Triggered, On-Demand Anti-inflammatories and Antibiotics Release by Graphene Oxide/Elecrospun PCL Patch for Wound Healing

2019 ◽  
Vol 5 (4) ◽  
pp. 63 ◽  
Author(s):  
Mauro ◽  
Cavallaro ◽  
Giammona
Keyword(s):  
Wound Healing ◽  
Graphene Oxide ◽  
Cell Adhesion ◽  
Near Infrared ◽  
Drug Loading ◽  
Controlled Drug Release ◽  
Infrared Light ◽  
Adhesion Properties ◽  
On Demand

Very recently, significant attention has been focused on the adsorption and cell adhesion properties of graphene oxide (GO), because it is expected to allow high drug loading and controlled drug release, as well as the promotion of cell adhesion and proliferation. This is particularly interesting in the promotion of wound healing, where antibiotics and anti-inflammatories should be locally released for a prolonged time to allow fibroblast proliferation. Here, we designed an implantable patch consisting of poly(caprolactone) electrospun covered with GO, henceforth named GO–PCL, endowed with high ibuprofen (5.85 mg cm−2), ketoprofen (0.86 mg cm−2), and vancomycin (0.95 mg cm−2) loading, used as anti-inflammatory and antibiotic models respectively, and capable of responding to near infrared (NIR)-light stimuli in order to promptly release the payload on-demand beyond three days. Furthermore, we demonstrated the GO is able to promote fibroblast adhesion, a key characteristic to potentially provide wound healing in vivo.

scholarly journals Mesh-like Electrospun Membrane Loaded With Atorvastatin Facilitates Cutaneous Wound Healing by Promoting The Paracrine Function Of Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Jieyu Xiang ◽  
Ling Zhou ◽  
Yuanlong Xie ◽  
Yufan Zhu ◽  
Lingfei Xiao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Growth Factor ◽  
Fourier Transform Infrared Spectroscopy ◽  
Drug Loading ◽  
The Other ◽  
Rt Pcr ◽  
Electrospun Membrane

Abstract Background:The Functional electrospun membrane was a promising strategy to promote wound healing, however, the microstructure and drug loading of the membranes still need further improvement. Here, we designed a novel mesh-like electrospun fibrous loaded with atorvastatin and investigated the effect on the paracrine secretion of BMSCs and wound healing.Methods:We fabricated a mesh-like electrospun membrane by using the copper mesh receiver. Physical properties of the membranes were characterized with Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, Tensile Strength Analysis and Contrast Angle Test. The drug Release was measured with a concentration curve of certain times. In vitro, we tested the effect of conditioned-medium (CM) from BMSCs on the migration and pro-angiogenic function of endothelial cells and used the real-time polymerase chain reaction (RT-PCR) to investigate the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) genes in BMSCs. Furthermore, a wound model of rat was established, the atorvastatin loaded mesh-like membranes (PCL/MAT) seeded with BMSCs were used as artificial skin to evaluate the wound-healing efficiency.Results:The Fourier Transform Infrared Spectroscopy reveals the successful loading of atorvastatin in mesh-like membranes (PCL/MAT). Compared with the random electrospun fibrous (PCL/R) and the mesh-like electruspun fibrous without drug-loading (PCL/M), PCL/MAT has the strongest effect on the migration of HUVECs. The scratches were almost closed after 24h of cell migration in the PCL/MAT group, whereas the scratches maintained a distance for the other groups. The effect of the PCL/MAT group was significant on the pro-angiogenic function of endothelial cells with obviously higher tube length than those of the other groups. And all above corresponding to the increased VEGF and b-FGF gene expression of BMSC in the RT-PCR. In vivo, PCL/MAT seeded with BMSCs significantly accelerated the speed of healing, improved the neovascularization and collagen reconstruction in the wound area compared with the other groups.Conclusions:Mesh-like topography of fibrous scaffolds with atorvastatin releasing can provide unique microenvironment that benefiting paracrine of BMSCs and migration and pro-angiogenic function of endothelial cells, thus accelerating wound healing. It's a novel strategy to design electrospun membranes for wound treatment

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