Effect of thrombocytopenia in patients with infective endocarditis: an insight from the National Inpatient Sample database

Abstract Introduction Infective endocarditis (IE) is one of the feared diseases in septic patients, and incidences are rising due to the intravenous drug abuse epidemic. Sepsis causes an escalation of the platelet destructions leading to thrombocytopenia (1). Few independent hospital-based studies have proposed increase mortality with thrombocytopenia in patients with IE (2–5). We aim to evaluate the significance of thrombocytopenia in IE subjects from the national inpatient sample (NIS) database. Method We analyzed the NIS database from Jan-2016 to Dec-2018 using Stata 16.0. NIS is the largest publicly available all-payer inpatient care database in the United States, containing data on more than seven million hospital stays per year. We identified patients with IE with or without thrombocytopenia using ICD-10 codes. The primary outcome of interest was in-hospital mortality comparison. We adjusted potential confounders (age, sex, diabetes, hypertension, etc.) with multivariate logistic regression analysis. Further analysis was done after balancing the population co-morbidity using a Greedy propensity match for accuracy. Results A total of 174,495 subjects were included in this study with a diagnosis of IE. Among these individuals, 33,285 patients had a concurrent diagnosis of thrombocytopenia. The mean ages were 53±19.5 years for the thrombocytopenia group and 55±19.8 years for others. Females were equally represented in both cohorts. There were 4,945 (14.86%) vs 2,835 (2.01%) mortalities reported in with and without thrombocytopenia group respectively. After propensity matching, there was a pronounced increase in mortality [Odds ratio (OR): 1.93 (1.72 – 2.15), p-value: <0.001] in the group with thrombocytopenia comparing to others. Complications such as Major bleeding requiring blood transfusion [OR: 1.45 (1.35–1.57)], acute myocardial infarction [OR: 1.56 (1.35–1.70)], complete heart block [OR: 1.44 (1.16–1.53)], cardiac arrest [OR: 1.44 (1.25–1.72)], acute respiratory failure [OR: 1.51 (1.39–1.73)] and pressor support requirement [(OR: 1.73 (1.57–2.01)] were notably higher in the cohort of thrombocytopenia with statistically significant p-value (<0.001). The difference in length of stay between both cohorts after propensity match wasn't statistically significant. Conclusion In conclusion, IE patients with thrombocytopenia have higher incidences of in-patient mortality and poor outcomes than cohort without thrombocytopenia. Some of the adverse consequences could be temporally explained by complications related to underlying thrombocytopenia. Further investigations are needed to delineate the outcome in this group of subjects. FUNDunding Acknowledgement Type of funding sources: None.

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Occurrence of Surgical Site Infections at a Tertiary Healthcare Facility in Abuja, Nigeria: A Prospective Observational Study

Medical Sciences ◽

10.3390/medsci6030060 ◽

2018 ◽

Vol 6 (3) ◽

pp. 60 ◽

Cited By ~ 2

Author(s):

Ahmed Olowo-okere ◽

Yakubu Ibrahim ◽

Ali Sani ◽

Busayo Olayinka

Keyword(s):

Kirschner Wire ◽

Surgical Site Infections ◽

Exploratory Laparotomy ◽

Healthcare Facility ◽

The United States ◽

Multivariate Logistic Regression Model ◽

P Value ◽

Surgical Interventions ◽

Co Morbidity ◽

Tertiary Healthcare

Surgical site infection (SSI) is one of the most frequent complications of surgical interventions. Several factors have been identified as major determinants of occurrence of SSIs. The present study determined the occurrence and possible risk factors associated with SSIs at a tertiary healthcare facility in Abuja, Nigeria. All patients scheduled for operation in the hospital during the study period and who consented to participate willingly in the study were observed prospectively for the occurrence of SSI based on criteria stipulated by the United States Centre for Disease Control and Prevention (CDC). Data on sociodemographic characteristics, lifestyle, surgical procedure and co-morbidity were collected into a pre-tested data collection tool and analysed using IBM SPSS Statistics software v.24. Predictors of SSIs were identified using multivariate logistic regression model and p-value less than 0.05 was considered statistically significant. Of the 127 surgical patients that met the inclusion criteria comprising 65 (51.2%) females and 62 (48.8%) males between 1 and 83 years with mean age of 25.64 ± 1.66 years, 35 (27.56%; 95% Confidence Interval (CI): 0.205–0.360) developed SSIs. Prolonged post-operative hospital stays (p < 0.05), class of wound (p < 0.0001) and some comorbid conditions were found to be significantly associated with higher SSI rate. The SSI rate was highest among patients that had Kirschner-wire insertion (75.0%), followed by an unexpectedly high infection rate among patients that had mastectomy (42.9%), while lower percentages (33.3%) were recorded among patients that had exploratory laparotomy and appendicectomy. The overall magnitude of SSIs in this facility is high (27.6%; 95% CI: 0.205–0.360). Several factors were found to be independent predictors of occurrence of SSI. The findings thus highlight the need for improved surveillance of SSIs and review of infection control policies of the hospital.

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Incidence and Outcomes of Acute Transfusion Reactions in Hospitalized Patients in the United States

Blood ◽

10.1182/blood-2020-138982 ◽

2020 ◽

Vol 136 (Supplement 1) ◽

pp. 30-31

Author(s):

Rohit Kumar ◽

Sindhu Malapati ◽

Sunny R K Singh ◽

Bokhodir Mamedov ◽

Myra R Shah ◽

...

Keyword(s):

Risk Factors ◽

Conflicts Of Interest ◽

Multivariate Logistic Regression Analysis ◽

The United States ◽

Hospitalized Patients ◽

P Value ◽

Continuous Variables ◽

Blood Products ◽

Increased Risk ◽

Transfusion Reactions

Introduction Acute transfusion reactions (ATRs) have a broad spectrum of presentations ranging from benign to life-threatening. Due to the rarity of these reactions, there is a paucity of data regarding their incidence and clinical outcomes. The objectives of this study were to determine the incidence of ATRs, its risk factors, and associated mortality. Methods: We reviewed the National Inpatient Sample (NIS) database 2014 for admissions where the patient (>=18 years old) was transfused blood products. The NIS is a large publicly available all-payer inpatient healthcare database designed to produce U.S. regional and national estimates of inpatient utilization, access, charges, quality, and outcomes. ATRs were identified using ICD-9 CM codes for transfusion-associated circulatory overload (TACO), transfusion-related acute lung injury (TRALI), febrile non-hemolytic transfusion reactions (FNHTR), acute infections, anaphylaxis, and acute hemolytic reaction. Pearson's chi-square and student's t-test were used to compare categorical and continuous variables between hospitalizations with versus without ATRs, respectively. Multivariate logistic regression analysis was done to determine the risk factors for common ATRs (TACO, TRALI, and FNHTR). A multivariate cox proportional model was built to compare the mortality of two study groups. A 2-sided p-value ≤ 0.05 was considered significant. Results: A total of 2,134,691 hospitalizations were associated with the transfusion of blood products. ATRs were documented in 0.2% of the hospitalizations (TACO 0.08%, TRALI 0.06%, FNHTR 0.09%, others 0.003%). The group that had ATRs was slightly younger (median age 67 vs 68 years, p=0.002), had the same proportion of females (58.3% vs 55.3%, p=0.055), less comorbidity score (28.7% vs 31.7% had Charlson Comorbidity Index >3, p=0.042) and more critically ill (17.8% vs 10.5% on mechanical ventilation, p<0.001) compared to group without ATRs. Hospitalizations with ATRs had longer median length of stay (7 vs 6 days, p<0.001) and higher median hospital cost ($64,399 vs $53,912, p<0.001) compared to without ATRs. The risk factors for common ATRs (odds ratio, OR) are mentioned in the table. ATRs were not associated with increased risk of mortality (combined HR 0.89 95%CI 0.71-1.12, p=0.321). Conclusions: Nationally, the incidence of ATRs is low in hospitalized patients and it is not associated with increased mortality. This large database analysis gives insight into the risk factors associated with different ATRs. Disclosures No relevant conflicts of interest to declare.

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Derajat Toksisitas Hemoglobin Pada Penderita Kanker Kolorektal Yang Mendapat Kemoterapi CapeOX

ARTERI : Jurnal Ilmu Kesehatan ◽

10.37148/arteri.v1i4.86 ◽

2020 ◽

Vol 1 (4) ◽

pp. 299-305

Author(s):

Yusmaidi ◽

Jordy Oktobiannobel ◽

Muhammad Nur ◽

Bella Sabila Dananda

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

The United States ◽

Hematological Toxicity ◽

P Value ◽

Chemotherapy Drugs ◽

Number Of Patients ◽

Significant Difference ◽

The Difference ◽

Colorectal Cancer Patients

Advances in the treatment and use of chemotherapy have been shown to improve the life expectancy rate for colorectal cancer patients. Studies conducted in China and Hongkong have shown that CapeOX combination chemotherapy regimens are more commonly used than in Europe and the United States. However, the use of chemotherapy drugs containing oxaliplatin and capecitabine can cause side effects such as hematological toxicity, which is one of them is anemia. This study aims to determine the difference in the form of a decrease in the average levels of hemoglobin and the degree of hemoglobin toxicity in colorectal cancer patients undergoing CapeOX chemotherapy. The Design in this study is a historical (retrospective) cohort. This study sample was 70 colorectal cancer patients who received CapeOX chemotherapy for 6 cycles at RSUD Dr. H. Abdul Moeloek in 2018-2019. Consecutive sampling is used in the sampling method. The statistical analysis is using Paired T-Test. There is a significant difference in the average hemoglobin level of colorectal cancer patients (p-value = <0.005), which receive CapeOX chemotherapy for 6 cycles. Besides, there is an increase in the number of patients who get hemoglobin toxicity and the chemotherapy cycle. In the first cycle, 59 patients (84.3%) got hemoglobin toxicity after chemotherapy, and the number continued to increase to 69 patients (98.6%) in the sixth cycle. There was a decrease in hemoglobin levels in colorectal cancer patients who received CapeOX chemotherapy with p-value = <0.05 and increased patients who got hemoglobin toxicity.

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Social determinants of poor outcomes following frostbite injury: a study of the National Inpatient Sample

Journal of Burn Care & Research ◽

10.1093/jbcr/irab115 ◽

2021 ◽

Author(s):

Frederick W Endorf ◽

Rachel M Nygaard

Keyword(s):

Medical Care ◽

Black Population ◽

The United States ◽

National Sample ◽

Homeless Population ◽

Admission Diagnosis ◽

National Inpatient Sample ◽

Factors Associated ◽

Poor Outcomes ◽

Functional Limb

Abstract Severe frostbite injury can result in significant disability from amputation of limbs and digits which may be mitigated through prompt medical care. The reported rates of amputation vary widely between centers. Our aim is to describe the incidence and factors associated with amputation secondary to frostbite injury in the United States using a national sample of hospitalizations. Admissions for frostbite injury were identified in the National Inpatient Sample (2016-18). Factors associated with amputation were assessed by multivariable logistic regression and clustered by hospital. The overall incidence of frostbite injury in the U.S. is 0.83/100,000 people. Of the social factors associated with frostbite injury, homelessness and Black race were independently associated with a higher likelihood of amputation at the primary admission. Diagnosis of cellulitis was a predictor of amputation. Homeless frostbite patients more frequently discharged AMA and were less likely to discharge with supportive medical care, despite having a higher rate of more severe injury. Disability from amputation following frostbite injury impacts at least 20% of frostbite injured patients and disproportionally impacts the homeless population. Further study is needed to ascertain the decision-making that leads to early amputation following frostbite injury, especially in the homeless and Black population. Outreach and education efforts should be initiated to promote salvage of functional limb length following frostbite injury.

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An outbreak of infective endocarditis during the COVID-19 pandemic? - an observational retrospective single centre study

European Heart Journal Acute Cardiovascular Care ◽

10.1093/ehjacc/zuab020.184 ◽

2021 ◽

Vol 10 (Supplement_1) ◽

Author(s):

M Cabral ◽

S Fernandes ◽

LG Santos ◽

R Carvalho ◽

FM Sa ◽

...

Keyword(s):

Diabetes Mellitus ◽

Logistic Regression ◽

Infective Endocarditis ◽

Univariate Analysis ◽

Multivariate Logistic Regression Analysis ◽

Early Mortality ◽

P Value ◽

Multivariate Logistic Regression ◽

Systemic Complications ◽

Duke Criteria

Abstract Funding Acknowledgements Type of funding sources: None. Infective endocarditis (IE) is described as an uncommon and challenging infective disease, due to its presentation variability. Its mortality remains high besides the better disease knowledge and therapeutic progress. The aim of this study was to describe the clinical features and the incidence of IE. Furthermore, we try to identify the risk factors associated with early mortality. A retrospective study was conducted and all patients diagnosed with definite or possible IE between January 2015 and June 2020, according to the modified Duke criteria, were included. Patient selection and information collection were obtained through medical records. Outcomes were in-hospital and 3 months after discharge mortality. Group comparisons and multivariate logistic regression analysis were performed. A p-value less than 0.05 is statistically significant. We analysed 51 patients, which 41 were admitted between 2015 and 2019 with a mean incidence of 8.2 cases per year. In 2020, 10 cases were diagnosed, corresponding to a percentual increase of 143.9%. Of the 51 patients, 70.6% were male. The mean age was 65 years. Fever was the main presentation feature at admission. We counted 33.3% prosthetic valve endocarditis (PVE) and 5.9% IE device-related. The most common pathogens were Streptococcus gallolyticus (13.7%) and Streptococcus oralis (13.7%). 35 patients (68.6%) had local complications and 26 patients (51%) had systemic complications. One-third of the patients was referenced to surgical treatment. Overall in-hospital mortality was 19.6% and early mortality at 3 months was 27.5%. In the univariate analysis, early mortality was higher in diabetes mellitus (DM) patients (p < 0.01) and in those who developed sepsis during hospitalization (p = 0.04). In multivariate logistic regression, only DM (OR = 15.8, 95% CI [3.2, 79.0]) was shown to be an independent factor of mortality. The incidence of IE increased during the first semester of 2020, possibly due to the increased attention given to patients with fever. Diabetes mellitus was found to be the only independent predictor of mortality. More national multicentre studies are needed. Predictive factors of 3 months mortalitySurvivors (n = 37)Non-survivors (n = 14)p-valueAge, med (IQ)70.0 (23.0)70.5 (14.0)0.42Female, n(%)11 (29.7)4 (28.6)1.00Diabetes mellitus, n(%)6 (16.2)11 (78.6)<0.01Local complication, n(%)24 (64.9)11 (78.4)0.50Sepsis, n(%)2 (5.4)4 (28.6)0.04Univariate analysis (resumed version). med-median, IQ-interquartile range, n-absolute frequencyAbstract Figure. ROC curve for logistic regression model

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Abstract 1122‐000053: Trends in Intervention Modality for Patients with Mycotic Aneurysms: A Nationwide Analysis

Stroke: Vascular and Interventional Neurology ◽

10.1161/svin.01.suppl_1.000053 ◽

2021 ◽

Vol 1 (S1) ◽

Author(s):

Giana Dawod ◽

Cenai Zhang ◽

Hang Shi ◽

Alexander E Merkler ◽

...

Keyword(s):

Infective Endocarditis ◽

Endovascular Treatment ◽

Intracranial Aneurysms ◽

The United States ◽

Sample Survey ◽

Treatment Modalities ◽

National Inpatient Sample ◽

Mycotic Aneurysms ◽

Over Time ◽

Infectious Intracranial Aneurysms

Introduction : Mycotic aneurysms, also known as infectious intracranial aneurysms, are sometimes responsible for intracranial hemorrhage in patients with infective endocarditis. Data regarding when and how to treat mycotic aneurysms most effectively are sparse. Given the widespread adoption of endovascular treatments for non‐infectious intracranial aneurysms and acute stroke, we hypothesized that endovascular treatment is increasingly utilized for patients with mycotic aneurysms. We examined trends in endovascular versus open neurosurgical treatment of mycotic aneurysms in patients with infective endocarditis. Methods : We performed a trends analysis using data from 2000–2015 from the National Inpatient Sample. The National Inpatient Sample is an all‐payer database that includes data for a representative sample of hospitalizations to non‐federal hospitals in the United States. We included all hospitalizations for patients with ruptured (on the basis of subarachnoid hemorrhage) or unruptured cerebral aneurysms alongside a diagnosis of infective endocarditis; diagnoses were ascertained using ICD‐9‐CM codes. Treatment modalities were categorized as endovascular versus open neurosurgical repair based on ICD‐9‐CM procedure codes. National Inpatient Sample survey weights were used to calculate nationally representative estimates. Logistic regression was used to evaluate the association between calendar year and intervention rate, presented as an odds ratio for each additional year. Results : We identified 1,015 hospitalizations for patients with a ruptured or unruptured cerebral aneurysm in the setting of infective endocarditis. Their mean age was 54.6 years (SD, 16.6), and 60.1% were male. The overall rate of intervention was 11.9% (95% CI, 9.6‐14.2%), and this rate did not change appreciably over time (p = 0.772). In comparing intervention modalities over time, there was a decrease in open neurosurgical repair (OR, 0.89; 95% CI, 0.84‐0.95; p = 0.001), offset by an increase in endovascular repair (OR, 1.07; 95% CI, 1.01‐1.14; p = 0.023) (Figure). Conclusions : Rates of mycotic aneurysm intervention during hospitalizations for infective endocarditis have not changed. However, the use of endovascular treatment has become more commonplace while the use of open neurosurgical treatments has decreased. Further directions include understanding whether this shift has improved patients’ outcomes and ultimately enumerating best practices for patients with mycotic aneurysms.

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Abstract 17334: Illicit Drug Users Infective Endocarditis: Winter Effect Causing Lowest Incidence but the Highest Mortality

Circulation ◽

10.1161/circ.138.suppl_1.17334 ◽

2018 ◽

Vol 138 (Suppl_1) ◽

Author(s):

Kartik Dhaduk ◽

Hardik Sonani ◽

Malav Patel ◽

Rushik Bhuva ◽

George Jolly ◽

...

Keyword(s):

Infective Endocarditis ◽

Drug Users ◽

Illicit Drug ◽

Multivariate Logistic Regression Analysis ◽

Seasonal Effect ◽

P Value ◽

Physical Factors ◽

Illicit Drug Users ◽

Congenital Cardiac Anomalies ◽

Incidence And Mortality

Introduction: Many cardiovascular diseases are known to have seasonal variation and that helps to better understand the disease. From the national sample, we studied the incidence and mortality pattern in infective endocarditis (IE) in illicit drug users (IDU) to delineate the seasonal effect on the disease. Methods: National inpatient sample (NIS) database from the year 2008 to 2014 was used to identify cases of IE and IDU (opiates, cocaine and amphetamine) in between age 15-65 years. After excluding cases with congenital cardiac anomalies, rheumatic, lupus and transfer status (possible doubling in count), data was analyzed using t-test, x 2 test and multivariate logistic regression analysis methods with SAS 9.4 software. Results: Out of total 144,593 identified cases of IE, 22,806 cases (15.77%) were illicit drug users infective endocarditis (IDU-IE). Patients in IDU-IE group were younger compare to non-IDU IE group (median± SE, p-value; 35± 0.39 vs 52± 0.11 yr, <0.001) but had higher longer hospital stay (12± 0.27 vs 10±0.1 days, <0.001). On seasonal pattern, highest hospitalisation was found in autumn (25.69%) followed by summer (25.23%), spring (22.8) and winter (19.5%) (figure1). In subgroup analysis, the most vulnerable population was in age group 15-35 years (49.8%), white race (71.8%) with Medicaid insurance (46.6%) in southern USA (37.6%). After adjusting for potential confounders in regression, mortality in winter was highest (aOR (95% CI), p-value; 1.40 (1.00-1.94), 0.04) (figure1). Old age >50 (2.43, (1.76-3.38), <0.001), >1 comorbidities (2.09 (1.59- 2.74), <0.001) and male (1.5 (1.18-1.91), <0.001) were predictors of higher mortality. Conclusion: In conclusion, drug use pattern is consistent with drug users IE pattern. But highest mortality despite of lowest incidence rate in winter suggests involvement of environmental and physical factors. Identifying these factors will help improving patient care and health care burden.

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Laterality of lower extremity deep vein thrombosis after colectomy: A retrospective study using the national inpatient sample

Phlebology The Journal of Venous Disease ◽

10.1177/0268355521994985 ◽

2021 ◽

pp. 026835552199498

Author(s):

Ivan E Saraiva ◽

Hirotaka Kato

Keyword(s):

Lower Extremity ◽

P Value ◽

National Inpatient Sample ◽

Chi Square ◽

Vein Thrombosis ◽

Left Lower Extremity ◽

Chi Square Test ◽

History Of ◽

The Difference ◽

Deep Vein

Background and aims Deep venous thrombosis (DVT) is known to occur preferentially on the left lower extremity. The renowned surgeon Denis Burkitt advanced the theory that a heavy sigmoid colon would compress the left pelvic veins and predispose to DVT. Our study aimed to evaluate this hypothesis by comparing the laterality distributions with and without a prior colectomy. Methods We conducted a retrospective analysis of the 2016 National Inpatient Sample database by stratifying the patients at any age with acute DVT of lower extremity by history of prior colectomy, thereby eliminating local gut mechanical factors in the development of DVT. We compared the laterality distribution (i.e., left, right, bilateral, and unspecified) between the patients with and without a prior colectomy. We also conducted a subgroup analysis by the sex category to examine the difference in laterality distribution for male and female patients. Chi-square test for independence was used. P value ≤0.05 was considered statistically significant. Results We found an estimated total of 342,525 cases. Among patients without a prior colectomy, 136,605 (41.6%) were left-sided DVT versus 119,555 (36.4%) right-sided, with 55,555 bilateral and 16,865 unspecified. Among patients with a prior colectomy, 5,750 (41.2%) were left-sided, 5,000 (35.9%) were right-sided, 2,345 were bilateral and 850 were unspecified. The laterality distribution between the two groups was not significantly different ( p = .167). The left-side predominance disappeared only in males with a prior colectomy (37.1% for left vs. 38.9% for right, p = .027). Conclusions Our findings did not confirm the Burkitt’s hypothesis. The left-side predominance of lower extremity DVT was attenuated only in male patients with a prior colectomy.

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Motivasi dan kepuasan kerja pegawai puskesmas BLUD dan non-BLUD Kabupaten Semarang

Health Sciences and Pharmacy Journal ◽

10.32504/hspj.v2i3.45 ◽

2018 ◽

Vol 2 (3) ◽

pp. 111

Author(s):

Aswindar Adhi Gumilang ◽

Tri Pitara Mahanggoro ◽

Qurrotul Aini

Keyword(s):

Public Health ◽

Job Satisfaction ◽

Financial Management ◽

Work Motivation ◽

P Value ◽

The Public ◽

The Status ◽

The Difference ◽

Demand For Health ◽

Public Demand

The public demand for health service professionalism and transparent financial management made some Puskesmas in Semarang regency changed the status of public health center to BLUD. The implementation of Puskesmas BLUD and non-BLUD requires resources that it can work well in order to meet the expectations of the community. The aim of this study is to know the difference of work motivation and job satisfaction of employees in Puskesmas BLUD and non-BLUD. Method of this research is a comparative descriptive with a quantitative approach. The object of this research are work motivation and job satisfaction of employees in Puskesmas BLUD and non-BLUD Semarang regency. This Research showed that Sig value. (P-value) work motivation variable was 0.019 smaller than α value (0.05). It showed that there was a difference of work motivation of employees in Puskemas BLUD and non-BLUD. Sig value (P-value) variable of job satisfaction was 0.020 smaller than α value (0.05). It showed that there was a difference of job satisfaction of BLUD and non-BLUD. The average of non-BLUD employees motivation were 76.59 smaller than the average of BLUD employees were 78.25. The average of job satisfaction of BLUD employees were 129.20 bigger than the average of non-BLUD employee were 124.26. Job satisfaction of employees in Puskesmas BLUD was higher than non-BLUD employees.

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Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

Jurnal Laboratorium Khatulistiwa ◽

10.30602/jlk.v1i2.147 ◽

2018 ◽

Vol 1 (2) ◽

pp. 114

Author(s):

Wahdaniah Wahdaniah ◽

Sri Tumpuk

Keyword(s):

Screening Test ◽

Venous Blood ◽

The Body ◽

Ethylene Diamine ◽

P Value ◽

Cross Sectional ◽

Significant Difference ◽

Ethylene Diamine Tetra Acetate ◽

The Difference ◽

Index Value

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk K2EDTA dan K3EDTA. K3EDTA biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

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