scholarly journals 130 Young Scholar Presentation: Can exogenous carbohydrase supplementation to higher-fiber diets improve gut function, microbiota, and growth performance of weaned pigs?

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 76-76
Author(s):  
Qingyun Li ◽  
Nicholas K Gabler ◽  
Eric R Burrough ◽  
Crystal L Loving ◽  
Stephan Schmitz-Esser ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bacterial Infections ◽  
Experimental Period ◽  
Nutrient Utilization ◽  
Enterotoxigenic Escherichia Coli ◽  
Gut Health ◽  
Weaned Pigs ◽  
Young Pigs ◽  
Gut Function ◽  
The Impact

Abstract There is increasing interest in feeding higher-fiber coproducts to weaned pigs due to their potential benefits on gut function and microbiota. However, young pigs are not efficient at utilizing fibrous coproducts. Exogenous carbohydrases can be used to improve nutrient utilization and growth of pigs fed higher levels of coproducts. Previous results regarding the impact of carbohydrases on performance in pigs have been inconsistent, thus a better understanding of associated mechanisms is needed. Using 460 weaned pigs (6.4 ± 0.1 kg), our first study showed that a carbohydrase enzyme blend (EB) improved ADG of weaned pigs fed higher-fiber diets (with added DDGS and wheat middlings) over a 28-d experimental period (P < 0.05). Pigs fed EB-supplemented diets had lower urinary lactulose:mannitol ratio, decreased plasma IL-8 concentration, and greater ileal CLDN3 (claudin 3) mRNA abundance, compared with those fed diets without EB (P < 0.05). These changes may partly explain the improved growth, providing mode of action evidence for carbohydrase in improving performance of weaned pigs. Carbohydrases may also exert prebiotic effects through release of oligosaccharides from fiber degradation. Thus, carbohydrases and dietary fiber may improve disease resilience of young pigs against bacterial infections, for example, enterotoxigenic Escherichia coli (ETEC) induced post-weaning diarrhea. Our second study evaluated the impact of soluble versus insoluble fiber with or without carbohydrases in newly weaned pigs (n = 60; 6.9 ± 0.07 kg) challenged with F18 ETEC. A diet containing a soluble and highly fermentable fiber from sugar beet pulp with added carbohydrases improved (P < 0.05) ADG, tended to increase (P < 0.10) ileal OCLN (occludin) mRNA, increased (P < 0.05) colonic OCLN mRNA, and tended to decrease (P < 0.10) ileal Escherichia-Shigella compared with ETEC-challenged control. Collectively, appropriate use of exogenous carbohydrases in higher-fiber diets (with the right type and amount of enzyme substrate) is promising in improving gut health and growth performance in weaned pigs.

PSIII-16 Growth Performance of Weaned Pigs Fed Barley Differing in Fermentable Starch and Fiber Profile

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 175-176
Author(s):  
Joaquin J Sanchez Zannatta ◽  
L F Wang ◽  
Eduardo Beltranena ◽  
Aaron D Beattie ◽  
Rex N Newkirk ◽  
...  
Keyword(s):  
Energy Source ◽  
Growth Performance ◽  
Steam Explosion ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Barley Grain ◽  
Gut Health ◽  
Weaned Pigs ◽  
Cereal Grain ◽  
Hulled Barley

Abstract Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P &lt; 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P &lt; 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P &lt; 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P &lt; 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.

scholarly journals Wheat bran fermented by mixed fungal strains improves the digestibility of crude fiber and may benefit the gut health without impacting the growth performance in weaned pigs

2021 ◽  
Vol 12 (7) ◽  
pp. 2962-2971
Author(s):  
Yuheng Luo ◽  
Jun He ◽  
Hua Li ◽  
Cong Lan ◽  
Jingyi Cai ◽  
...  
Keyword(s):  
Growth Performance ◽  
Wheat Bran ◽  
Nutrient Digestibility ◽  
Crude Fiber ◽  
Gut Health ◽  
Intestinal Health ◽  
Weaned Piglets ◽  
Weaned Pigs ◽  
Fungal Strains

This study was conducted to compare the effect of raw (WB) or mixed fungi-fermented wheat bran (FWB) on the growth, nutrient digestibility and intestinal health in weaned piglets.

scholarly journals 173 Lysed Corynebacterium glutamicum cell mass from lysine production as a novel feed additive to enhance gut health and growth of newly-weaned pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 77-78
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim
Keyword(s):  
Growth Performance ◽  
Corynebacterium Glutamicum ◽  
Block Design ◽  
Cell Mass ◽  
Nutrient Digestibility ◽  
Feed Additive ◽  
Jejunal Mucosa ◽  
Gut Health ◽  
Weaned Pigs ◽  
Dietary Treatments

Abstract The objective was to determine the functional and nutritional values of Corynebacterium glutamicum Cell Mass (CGCM) on growth performance and gut health of newly-weaned pigs. Forty newly-weaned pigs (21 d of age; initial BW 7.1 ± 0.4 kg) were allotted to 5 dietary treatments based on randomized complete block design with sex and BW as blocks. The lysine broth of CGCM (CJ Bio, Fort Dodge, IA) was homogenized by using French press and dried to obtain lysed CGCM. Dietary treatments were: basal diet with lysed CGCM at 0, 0.7, 1.4, 2.1%, and with 1.4% intact CGCM. Experimental diets were formulated based on nutrient requirements (NRC, 2012) and pigs were fed based on 2 phases (10 and 11 d for each phase). Titanium dioxide (0.4%) was added to phase 2 diets as an indigestible external marker to calculate nutrient digestibility. Feed intake and BW were measured at d 0, 10, and 21. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure TNF-α, IL-8, MDA, IgA, and IgG concentrations. Diets and ileal digesta were collected to measure AID. Data were analyzed by SAS using MIXED, REG, and GLM procedures. Overall, increasing daily lysed CGCM intake increased (P &lt; 0.05) ADG (211 to 296 g) and ADFI (432 to 501 g). Increasing levels of lysed CGCM decreased (P &lt; 0.05) MDA and changed (quadratic, P &lt; 0.05) IgA (max: 4.90 ng/mg at 1.13%) and IgG (max: 3.37 ng/mg at 1.04%) in the proximal jejunal mucosa. Increasing daily lysed CGCM intake had quadratic effect (P&lt; 0.05) of protein carbonyl (max: 6.3 μmol/mg at 4.9 g/d). Lysed CGCM potentially benefits growth performance and gut health of newly-weaned pigs by reducing oxidative stress and increasing immune response.

195 Does Feeding a Protease and Probiotic (Bacillus Subtilis) Combination (Syncra® SWI) Result in Improved Growth Performance and Mortality in a Commercial Wean-to-finish Swine Feeding Program?

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 77-78
Author(s):  
Trey A Kellner ◽  
Josh Ellingson ◽  
Ana L P de Souza ◽  
Janet C Remus
Keyword(s):  
Growth Performance ◽  
Experimental Period ◽  
Feed Additives ◽  
Control Treatment ◽  
Experimental Unit ◽  
Gut Health ◽  
Wide Range ◽  
Feeding Program ◽  
Feed Costs ◽  
Combined Feed

Abstract The response to probiotics and enzymes is often documented in research facilities with a high degree of control and via pigs with no insults to health or feed intake. However, in commercial conditions, the response to feed additives promoting advanced gut health and improved digestion must be consistent and defined over a wide range of health statuses, stocking densities, feed intakes, environments, and diet formulations. The objective of this experiment was to determine if a combined feed protease and probiotic system (Syncra® SWI 201, DuPont, Wilmington, DE) would improve growth performance and mortality under commercial conditions. A total of 127,092 pigs (6.0 ± 0.1 kg; PIC 337 sired, Hendersonville, TN) from a sow farm producing porcine reproductive and respiratory virus and rotavirus positive weaned pigs were placed in 53 2,400-head commercial wean-to-finish barns that were alternated to 1 of 2 treatments (a control treatment without Syncra® SWI (SSWI) or with SSWI included at 72.6 g/ton of finished feed from 22.7 kg of BW to harvest) in a rolling allotment over a 12-month period. Pigs were on the experiment for an average of 162 ± 1.0 days (until harvest). Throughout the 12-month experimental period, diets (outside of the SSWI inclusion) could change in order to maximize return over feed costs. Data were analyzed using Proc MIXED (SAS 9.4; Cary, NC) with treatment as the main effect and barn as the experimental unit. Compared to the control, adding SSWI improved mortality by 1.9% and percent grade 1 marketed pigs by 2.2% (P ≤ 0.025). Compared to the control, SSWI did not improve ADG (control = 0.75 vs. SSWI = 0.78 kg) or gain:feed (control = 0.401 vs. SSWI = 0.396). In conclusion, the inclusion of the SSWI combined feed protease and probiotic system can improve mortality and grade 1 marketed pigs, but not growth performance under commercial conditions.

scholarly journals Changing Dietary Calcium-Phosphorus Level and Cereal Source Selectively Alters Abundance of Bacteria and Metabolites in the Upper Gastrointestinal Tracts of Weaned Pigs

2013 ◽  
Vol 79 (23) ◽  
pp. 7264-7272 ◽  
Author(s):  
Barbara U. Metzler-Zebeli ◽  
Evelyne Mann ◽  
Stephan Schmitz-Esser ◽  
Martin Wagner ◽  
Mathias Ritzmann ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Health ◽  
Weaned Pigs ◽  
Fermentation Products ◽  
Content Type ◽  
Young Pigs ◽  
Bacterial Microbiota ◽  
Calcium Phosphorus ◽  
And Performance ◽  
The Stability

ABSTRACTSeveral dietary ingredients may affect the bacterial community structure and metabolism in the porcine gut and may therefore influence animals' health and performance. This study investigated the effects of cereal source and calcium-phosphorus (CaP) level in the diet on bacterial microbiota and metabolites, nutrient intake, and gut environment in weaned pigs. Pigs (n= 8/treatment) were fed wheat-barley- or corn-based diets with an adequate or high CaP level for 14 days. Effects on microbiota in the stomach, ileum, and midcolon were assessed using quantitative PCR. Data showed thatEnterobacteriaceae,Campylobacterspp., andHelicobacterspp., which all contain highly immune reactive lipopolysaccharide (LPS), were abundant at all gut sites. Diet effects on bacteria and metabolites were moderate and occurred mainly in the upper gut, whereas no effects on bacteria, fermentation products, and LPS could be observed in the colon. Differences in carbohydrate intake with corn versus wheat-barley diets selectively stimulatedBifidobacteriumin the stomach and ileum. There was a growth advantage for a few bacterial groups in the stomach and ileum of pigs fed the high versus adequate CaP level (i.e., gastricEnterobacteriaceaeand ilealEnterococcus,Bacteroides-Prevotella-Porphyromonas, andCampylobacter). Interestingly, gastrointestinal pH was not affected by dietary CaP level. The present findings demonstrate the stability of the bacterial community and gut environment toward dietary changes even in young pigs. The results on stimulation of gastric and ilealBifidobacteriumby corn diets may be employed in nutritional strategies to support gut health after weaning.

The impacts of a fibrolytic enzyme additive on digestibility and performance in the grower period, and supplemental Saccharomyces cerevisiae on performance and rumen health in the finisher period for feedlot steers

2021 ◽  
Author(s):  
Melissa S. Williams ◽  
Ousama AlZahal ◽  
Ira B. Mandell ◽  
Brian McBride ◽  
Katie Wood
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Performance ◽  
Mrna Expression ◽  
Experimental Treatment ◽  
Gut Health ◽  
Feed Conversion ◽  
Ruminal Ph ◽  
Gut Barrier Function ◽  
And Performance ◽  
The Impact

Two experiments were conducted to determine the effects of a fibrolytic enzyme pretreatment on growth performance, apparent total tract digestibility, and ruminal pH throughout the grower and early finisher period (Exp. 1), and to examine the impact of Saccharomyces cerevisiae supplementation on intake, performance, and indicators of gut health in the late finisher period (Exp. 2). Fifty-four steers were randomly assigned to a subgroup determining experimental treatment groups. In Exp. 1, steers were randomized to: control (CON1; no enzyme) or enzyme (ENZ; 0.75 ml kg-1 DM of feed) dietary treatments. Digestibility was improved (P≤0.05) in ENZ steers for DM, CP, NEg, and sugars, but did not affect (P≥0.12) DMI, ADG, or reticulo-ruminal pH. In Exp. 2, the treatments were: control (CON2; no yeast) or yeast (YST; 3.0 g animal-1 daily) supplemented diets. Rumen papillae were collected for mRNA expression of gut barrier function (OCLN, CLDN, ZO1, ZO2) and immune response (TLR2, TLR4, FCAR) genes and histological measurements. YST decreased (P<0.001) DMI by 31%, reduced variation in dry matter intake, and improved feed conversion ratios, but did not impact rumen health mRNA expression or histology measures (P≥0.07). Overall, ENZ improved the digestibility of some nutrients in the grower period, and YST improved feed efficiency, without impacting growth performance or gut health.

scholarly journals Effects of super-dosing phytase and inositol on growth performance and blood metabolites of weaned pigs housed under commercial conditions1

2019 ◽  
Vol 97 (7) ◽  
pp. 3007-3015 ◽  
Author(s):  
Kory Moran ◽  
Pete Wilcock ◽  
Amanda Elsbernd ◽  
Cate Zier-Rush ◽  
R Dean Boyd ◽  
...  
Keyword(s):  
Growth Performance ◽  
Phase 1 ◽  
Weaned Pigs ◽  
Phase 3 ◽  
Young Pigs ◽  
Weaning Stress ◽  
Serum Zn ◽  
Feeding Program ◽  
Essential Nutrient ◽  
Dietary Treatments

Abstract A total of 2,156 weaned pigs (6.75 ± 0.11 kg BW) were used in a 42-d study to evaluate whether improvements in growth performance associated with super-dosing phytase can be explained by the complete dephosphorylation of phytate and liberation of inositol. Two phytase doses (0 and 2,500 FTU/kg) and 3 inositol concentrations (0%, 0.15%, and 0.30%) were combined to create 6 dietary treatments in a 2 × 3 factorial arrangement. Pigs were fed a 3-phase feeding program, with periods being 10, 10, and 22 d, respectively. Blood samples were collected on days 0, 21, and 42 from a subset of 48 pigs to analyze mineral and myo-inositol concentrations. During Phase 1, super-dosing phytase tended to improve ADG compared with pigs fed diets without phytase (P = 0.09). Increasing concentrations of inositol improved the efficiency of gain in pigs fed diets without phytase (1,022.1, 1,040.9, and 1,089.2 g/kg), but not diets with phytase (1,102.2, 1,087.2, and 1,076.2 g/kg), and this improvement was equivalent to that observed with super-dosing phytase in the absence of inositol (interaction, P = 0.015). During Phase 2, super-dosing phytase improved ADG (P = 0.001), resulting in heavier BW (P = 0.007). During Phase 3 and overall, inositol supplementation increased ADG and ADFI in a quadratic manner (P < 0.10), with the highest ADG and ADFI observed for pigs fed 0.15% of inositol. Super-dosing phytase increased serum Zn on day 21, but not on day 42 (interaction, P = 0.008), increased serum Cu (P = 0.01), but decreased serum Fe (P = 0.02). Plasma myo-inositol increased linearly from 66.9 to 97.1 and 113.2 nmol/mL with increasing inositol (P < 0.001). When plasma myo-inositol was analyzed within the subgroup of pigs fed diets without added inositol, super-dosing phytase increased plasma myo-inositol from 57.81 to 76.05 nmol/mL (0 and 2,500 FTU/kg, respectively; P = 0.05). Results demonstrate that exogenous inositol improved efficiency of gain in weaned pigs to the same level as that observed with super-dosing phytase, but this occurred only during the first 10 d of the nursery period. This suggests that the improvement in efficiency of growth when applying super-dosing phytase could be linked, in part, to complete dephosphorylation of phytate and liberation of myo-inositol, and that myo-inositol had a greater metabolic impact in piglets immediately after weaning. Consequently, myo-inositol may be a conditionally essential nutrient for young pigs during weaning stress, but further research is needed to prove this hypothesis.

scholarly journals 289 Effects of supranutritional levels of dietary zinc oxide on zinc, copper, and iron metabolism in post-weaned pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 106-107
Author(s):  
Danyel Bueno Dalto ◽  
Frédéric Guay ◽  
Yan Martel-Kennes ◽  
Guylaine Talbot ◽  
Martin Lessard ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Serum Concentration ◽  
Treatment Effects ◽  
Experimental Period ◽  
Dietary Zinc ◽  
Weaned Pigs ◽  
Cu Content ◽  
Cu Deficiency ◽  
Fe Homeostasis ◽  
The Impact

Abstract This study evaluated the impact of supranutritional levels of dietary zinc oxide (ZnO) on zinc (Zn), copper (Cu), and iron (Fe) homeostasis in post-weaned pigs. One hundred twenty piglets were selected at d14 of age (5.65 ± 0.68 kg). At weaning (d21), they were fed a basal post-weaning diet (without antibiotics) supplemented (n = 40/treatment) with 100 mg/kg (low; LZn), 1000 mg/kg (medium; MZn) or 3000 mg/kg (high; HZn) of ZnO until d42. Dietary levels of Cu (130 mg/kg) and Fe (260 mg/kg) remained constant throughout the experimental period. Piglets were sacrificed at d21, d23, d35, and d42 of age (n = 10/treatment/d) for samples collection. Serum concentrations and total liver content of Zn and Cu were not different among treatments on d21 and d23 (P ≥ 0.10). For Zn, treatment effects were detected at d35 and d42 (P &lt; 0.01) on both serum concentration and total Zn content in liver. For both parameters, values were greatest for HZn, intermediary for MZn, and lowest for LZn irrespective of ages in the two periods. For Cu, treatment effects were detected at d35 and d42 (P &lt; 0.01) on both serum concentration and total Cu content in liver. For both parameters, values were greatest for LZn and MZn and lowest for HZn irrespective of ages. For Fe, no treatment effect was detected either on whole blood Fe concentrations (P = 0.81) or hemoglobin levels (P = 0.76). However, for total Fe content in liver, treatment effects were detected (P &lt; 0.01). Values were greatest for LZn and lowest for HZn at d35 and d42 whereas for MZn they were intermediary at d35 and similar to LZn at d42 (P ≤ 0.01). In conclusion, supranutritional levels of dietary ZnO fed to piglets during 3 weeks post-weaning significantly impacted Zn, Cu, and Fe homeostasis. These results emphasise the potential risk of Cu deficiency as well as impairment in building body Fe reserves under long-term supranutritional supplementation of ZnO.

Effect of antibiotic-free, low-protein diets with specific amino acid compositions on growth and intestinal flora in weaned pigs

2020 ◽  
Vol 11 (1) ◽  
pp. 493-507 ◽  
Author(s):  
Junyan Zhou ◽  
Yuming Wang ◽  
Xiangzhou Zeng ◽  
Tao Zhang ◽  
Peili Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Intestinal Flora ◽  
Amino Acid Profile ◽  
Gut Health ◽  
Weaned Pigs ◽  
Specific Amino Acid ◽  
Amino Acid Compositions ◽  
Low Protein ◽  
Protein Diets

This study investigated the effects of modulation of the amino acid profile on growth performance and gut health in weaned pigs fed an antibiotic-free, low-protein diet.

Fructooligosaccharides improve growth performance and intestinal epithelium function in weaned pigs exposed to enterotoxigenic Escherichia coli

2020 ◽  
Vol 11 (11) ◽  
pp. 9599-9612
Author(s):  
Lei Liu ◽  
Daiwen Chen ◽  
Bing Yu ◽  
Heng Yin ◽  
Zhiqing Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Intestinal Epithelium ◽  
Intestinal Injury ◽  
Enterotoxigenic Escherichia Coli ◽  
Weaned Pigs ◽  
Protective Potential

This study was conducted to explore the protective potential of fructooligosaccharides (FOS) against enterotoxigenic Escherichia coli (ETEC)-induced inflammation and intestinal injury in weaned pigs.

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