scholarly journals 15 Dietary supplementation with lactic acid and glutamine improve intestinal digestive and regulate intestinal microflora in weaning piglets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 16-16
Author(s):  
Ping Zheng ◽  
Junjie Jiang ◽  
Bing Yu ◽  
Jun He ◽  
Jie Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Growth Performance ◽  
Basal Diet ◽  
Jejunal Mucosa ◽  
Mrna Levels ◽  
Intestinal Development ◽  
Serum Growth Hormone ◽  
Weaning Piglets ◽  
Dietary Treatments

Abstract This study aimed to evaluate the effects of dietary addition of lactic acid and glutamine on growth performance and intestinal health in weaning pigs. Ninety six 24-d-old piglets (Duroc × Landrace × Yorkshire) with BW of 7.24± 0.09 kg were randomly assigned to 1 of 4 dietary treatments: 1) basal diet (CON), 2) CON supplemented with 2% lactic acid (LS), 3) CON supplemented with 1% glutamine (GS), and 4) CON supplemented with 2% lactic acid and 1% glutamine (LGS). After 28-d trial, 6 piglets from each treatment were randomly selected to collect serum and intestinal samples. The results showed that piglets fed the LGS had a greater (P < 0.05) ADG than piglets fed the CON diet. Compared with CON, apparent total tract digestibility of CP in LS, GS and LGS was greater (P < 0.05), index of diarrhea in LS and LGS was lower (P < 0.05), serum IGF-1 in LS, GS and LGS and serum growth hormone in LS and GS were improved (P < 0.05). Furthermore, jejunal villus height in LS and LGS were increased (P < 0.05), the activities of amylase and lipase of the jejunal mucosa in LS and LGS were increased (P < 0.05). In addition, compared with CON, pigs fed the LS, GS and LGS up-regulated (P < 0.05) the mRNA levels of glucose transport type 2, IGF-1, occludin. Moreover, LGS had higher (P < 0.05) abundances of total bacteria, Lactobacillus and Bifidobacterium in cecal and colonic digesta, and higher concentrations of total volatile fatty acid in cecal digesta. Collectively, the current results indicate that dietary addition of lactic acid and glutamine combination could improve growth performance through the promotion of the small intestinal development, increasing digestive, and regulating balances of microflora in piglets.

scholarly journals Probiotics and Achyranthes bidentata Polysaccharides Improve Growth Performance via Promoting Intestinal Nutrient Utilization and Enhancing Immune Function of Weaned Pigs

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2617
Author(s):  
Gaifeng Hou ◽  
Wei Peng ◽  
Liangkai Wei ◽  
Rui Li ◽  
Xingguo Huang ◽  
...  
Keyword(s):  
Immune Function ◽  
Growth Performance ◽  
Basal Diet ◽  
Nutrient Utilization ◽  
Jejunal Mucosa ◽  
Weaned Pigs ◽  
Achyranthes Bidentata ◽  
Dietary Treatments ◽  
Positive Effect ◽  
Nutrients Digestibility

The experiment aimed to investigate the effects of probiotics and Achyranthes bidentata polysaccharides on the growth performance, nutrients digestibility, and immune function of weaned pigs. One hundred and twenty weaned pigs (about 7 kg BW, 23 ± 2 d) were allotted to five dietary treatments (CON: antibiotics-free basal diet; ANT: CON + antibiotics; PRO: CON + probiotics; ABPS: CON + Achyranthes bidentata polysaccharides; P-ABPS: PRO + ABPS) for a 28-day trial. Compared with CON, pigs in ANT, PRO, ABPS, and P-ABPS had greater (p < 0.05) ADG, ATTD of CP and GE, serum ALB, IgA and IL-2, duodenal intraepithelial lymphocyte, ileal VH and jejunal mucosa sIgA, but lower (p < 0.05) fecal scores, serum BUN, and IL-1β. Meanwhile, ANT, PRO, ABPS, and P-ABPS exhibited similar beneficial roles on growth performance, nutrients digestibility, serum parameters, and immune function. Interestingly, P-ABPS effects were similar to those obtained with ANT rather than with PRO or ABPS. In conclusion, Dietary PRO or ABPS used alone or in combination (P-ABPS), the combination augmenting the positive effect more than the independent supplement, could improve piglets’ growth performance via promoting intestinal nutrient digestion and absorption and enhancing immune function, indicating it had the potential to act as an alternative to in-feed antibiotics used in piglet diets.

scholarly journals 179 Efficacy and safety of amino acids with biomass for growth and health of newly-weaned pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 77-77
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Function Analysis ◽  
Basal Diet ◽  
Jejunal Mucosa ◽  
Phase 2 ◽  
Efficacy And Safety ◽  
Gut Health ◽  
Weaned Pigs ◽  
Dietary Treatments

Abstract The objective was to test the efficacy of L-Thr or L-Val with biomass (ThrPro or ValPro; CJ BIO, Fort Dodge, IA) on growth performance and health compared with L-Thr or L-Val (purified) in newly-weaned pigs. There were 9 dietary treatments based on NRC (2012) requirements: NC (basal diet meeting 70% of requirements for SID Thr and Val); 4 treatments based on basal diet meeting 95% of requirements: LT (with L-Thr), LV (with L-Val), PT (with ThrPro), and PV (with ValPro); HLT (fivefold L-Thr in LT), HLV (fivefold L-Val in LV), HPT (fivefold ThrPro in PT), and HPV (fivefold ValPro in PV). Pigs were fed for 26 d in 2 phases. Feed intake and BW were measured at d 0, 5, 10, 17, and 26. Blood was collected on d 21 for liver function analysis. Pigs were euthanized on d 26 to collect jejunal mucosa for gut health parameters. Data were analyzed by SAS using MIXED procedure. Pigs fed LT and LV or PT and PV had higher (P &lt; 0.05) ADG than pigs fed NC during phase 2. Pigs fed LT, LV, PT, and PV had higher ADFI and G:F than pigs fed NC during d 17 to 26. Pigs fed PT and PV had lower (P &lt; 0.05) G:F than pigs fed HPT and HPV during phase 2. Pigs fed LT and LV or PT and PV had lower (P &lt; 0.05) BUN than pigs fed NC. Overall, pigs fed PT and PV had no differences in any analyzed measurements from pigs fed LT and LV and pigs fed HPT and HPV. In conclusion, PT and PV were equally efficient to LT and LV for growth performance and health. HPT and HPV did not impair liver and gut health compared with PT and PV.

scholarly journals Effects of Dietary β-Mannanase Supplementation on Growth Performance, Apparent Total Tract Digestibility, Intestinal Integrity, and Immune Responses in Weaning Pigs

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 703
Author(s):  
Jae-Cheol Jang ◽  
Kwang Kim ◽  
Young Jang ◽  
Yoo Kim
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Growth Performance ◽  
Basal Diet ◽  
Villus Height ◽  
Crypt Depth ◽  
Intestinal Integrity ◽  
Weaning Pigs ◽  
Dietary Treatments ◽  
And Oxidative Stress

The experiment aimed to investigate the effects of dietary β-mannanase supplementation on growth performance, apparent total tract digestibility (ATTD) of nutrients, intestinal integrity, and the immunological and oxidative stress parameters in weaning pigs. A total of 64 newly weaning pigs (initial body weight: 6.96 ± 0.70 kg) were allotted to two dietary treatments in eight replicates per treatment with four pigs per pen based on body weight and sex. Dietary treatments were 1.) CON (control: corn-soybean meal based basal diet) and 2.) β-mannanase (basal diet +0.06% β-mannanase). The β-mannanase supplementation did not affect growth performance, concentrations of acute phase protein, superoxide dismutase and glutathione peroxidase. However, the pigs fed the β-mannanase-supplemented diet had greater ATTD of ether extract, jejunum villus height, and villus height-to-crypt depth ratio, and lower crypt depth compared with those fed the CON diet (p < 0.05). The pigs fed the β-mannanase-supplemented diet tended to have the lower count of E. coli in cecum than those fed the CON diet (p = 0.08). In conclusion, dietary β-mannanase supplementation did not affect growth performance, immune response and oxidative stress of weaning pigs, whereas it increased fat digestibility and had positive effects on intestinal integrity and cecum microflora by reducing the count of E.coli.

scholarly journals Effects of Substitution of Corn with Ground Brown Rice on Growth Performance, Nutrient Digestibility, and Gut Microbiota of Growing-Finishing Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 375
Author(s):  
Sheena Kim ◽  
Jin Ho Cho ◽  
Younghoon Kim ◽  
Hyeun Bum Kim ◽  
Minho Song
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Block Design ◽  
Brown Rice ◽  
Carcass Characteristics ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Finishing Pigs ◽  
Dietary Treatments

The present study was conducted to evaluate the effects of replacing corn with brown rice on growth performance, nutrient digestibility, carcass characteristics, and gut microbiota of growing and finishing pigs. A total of 100 growing pigs (23.80 ± 2.96 kg BW; 10 weeks of age) were randomly allotted to 4 dietary treatments (5 pigs/pen; 5 replicates/treatment) in a randomized complete block design (block = BW) as follows: corn-soybean meal basal diet (CON) and replacing corn with 50% (GBR50), 75% (GBR75), and 100% (GBR100) of ground brown rice. Each trial phase was for 6 weeks. During the growing period, there were no differences on growth performance and nutrient digestibility among dietary treatments. Similarly, no differences were found on growth performance, nutrient digestibility, and carcass characteristics of pigs during the finishing period among dietary treatments. As a result of the beta diversity analysis, microbial populations were not clustered between CON and GBR100 during the growing phase, but clustered into two distinct groups of CON and GBR100 during the finishing phase. In conclusion, brown rice can be added to the diets of growing-finishing pigs by replacing corn up to 100% without negatively affecting growth performance of the pigs; additionally, this may have an effect on changes in pig intestinal microbiota if continued for a long time.

scholarly journals 173 Lysed Corynebacterium glutamicum cell mass from lysine production as a novel feed additive to enhance gut health and growth of newly-weaned pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 77-78
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim
Keyword(s):  
Growth Performance ◽  
Corynebacterium Glutamicum ◽  
Block Design ◽  
Cell Mass ◽  
Nutrient Digestibility ◽  
Feed Additive ◽  
Jejunal Mucosa ◽  
Gut Health ◽  
Weaned Pigs ◽  
Dietary Treatments

Abstract The objective was to determine the functional and nutritional values of Corynebacterium glutamicum Cell Mass (CGCM) on growth performance and gut health of newly-weaned pigs. Forty newly-weaned pigs (21 d of age; initial BW 7.1 ± 0.4 kg) were allotted to 5 dietary treatments based on randomized complete block design with sex and BW as blocks. The lysine broth of CGCM (CJ Bio, Fort Dodge, IA) was homogenized by using French press and dried to obtain lysed CGCM. Dietary treatments were: basal diet with lysed CGCM at 0, 0.7, 1.4, 2.1%, and with 1.4% intact CGCM. Experimental diets were formulated based on nutrient requirements (NRC, 2012) and pigs were fed based on 2 phases (10 and 11 d for each phase). Titanium dioxide (0.4%) was added to phase 2 diets as an indigestible external marker to calculate nutrient digestibility. Feed intake and BW were measured at d 0, 10, and 21. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure TNF-α, IL-8, MDA, IgA, and IgG concentrations. Diets and ileal digesta were collected to measure AID. Data were analyzed by SAS using MIXED, REG, and GLM procedures. Overall, increasing daily lysed CGCM intake increased (P &lt; 0.05) ADG (211 to 296 g) and ADFI (432 to 501 g). Increasing levels of lysed CGCM decreased (P &lt; 0.05) MDA and changed (quadratic, P &lt; 0.05) IgA (max: 4.90 ng/mg at 1.13%) and IgG (max: 3.37 ng/mg at 1.04%) in the proximal jejunal mucosa. Increasing daily lysed CGCM intake had quadratic effect (P&lt; 0.05) of protein carbonyl (max: 6.3 μmol/mg at 4.9 g/d). Lysed CGCM potentially benefits growth performance and gut health of newly-weaned pigs by reducing oxidative stress and increasing immune response.

scholarly journals Dietary purslane (Portulaca oleracea L.) promotes the growth performance of broilers by modulation of gut microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cong Wang ◽  
Qing Liu ◽  
Fengchun Ye ◽  
Hongbo Tang ◽  
Yanpeng Xiong ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Basal Diet ◽  
Scientific Basis ◽  
Control Group ◽  
Good Growth ◽  
Feed Conversion ◽  
Illumina Hiseq ◽  
Treatment Groups ◽  
Dietary Treatments

AbstractPurslane is a widespread wild vegetable with both medicinal and edible properties. It is highly appreciated for its high nutritional value and is also considered as a high-quality feed resource for livestock and poultry. In this study, Sanhuang broilers were used to investigate the effect of feeding purslane diets on the growth performance in broilers and their gut microbiota. A total of 48 birds with good growth and uniform weight were selected and randomly allocated to four treatment groups A (control), B, C and D. Dietary treatments were fed with basal diet without purslane and diets containing 1%, 2% and 3% purslane. The 16S rDNA was amplified by PCR and sequenced using the Illumina HiSeq platform to analyze the composition and diversity of gut microbiota in the four sets of samples. The results showed that dietary inclusion of 2% and 3% purslane could significantly improve the growth performance and reduce the feed conversion ratio. Microbial diversity analysis indicated that the composition of gut microbiota of Sanhuang broilers mainly included Gallibacterium, Bacteroides and Escherichia-Shigella, etc. As the content of purslane was increased, the abundance of Lactobacillus increased significantly, and Escherichia-Shigella decreased. LEfSe analysis revealed that Bacteroides_caecigallinarum, Lachnospiraceae, Lactobacillales and Firmicutes had significant differences compared with the control group. PICRUSt analysis revealed bacteria mainly enriched in carbohydrate metabolism pathway due to the additon of purslane in the diet. These results suggest that the addition of purslane to feed could increase the abundance of Lactobacillus in intestine, modulate the environment of gut microbiota and promote the metabolism of carbohydrates to improve its growth performance. This study indicates that the effect of purslane on the growth-promoting performance of broilers might depend on its modulation on gut microbiota, so as to provide a certain scientific basis for the application of purslane in the feed industry.

187 Effects of Oligosaccharide-based Polymer on Growth Performance, Diarrhea, and Fecal β-hemolytic Coliforms in Weanling Pigs Experimentally Infected with a Pathogenic E. coli

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 90-90
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Seijoo Yang ◽  
Xunde Li ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Basal Diet ◽  
Watery Diarrhea ◽  
Experimental Unit ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
E Coli ◽  
Diet Control ◽  
Dietary Treatments

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Growth performance was measured on d -7 to 0 before inoculation, and d 0 to 5 and 5 to 11 post-inoculation (PI). Diarrhea score (DS; 1, normal, to 5, watery diarrhea) was daily recorded for each pig. Fecal samples were collected on d 2, 5, 8, and 11 PI to test the percentage of β-hemolytic coliforms in total coliforms. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Inclusion of oligosaccharide-based polymer linearly increased (P &lt; 0.05) ADFI on d 0 to 5 PI, and feed efficiency on d 0 to 5 PI and d 5 to 11 PI (P = 0.07), compared with the control. Supplementation of AGP or oligosaccharide-based polymer reduced (P &lt; 0.01) frequency of diarrhea of pigs from d 0 to 11 PI. No differences were observed in overall growth performance and percentage of fecal β-hemolytic coliforms on d 8 PI among pigs in AGP and oligosaccharide-based polymer treatments. In conclusion, supplementation of oligosaccharide-based polymer enhanced feed efficiency and reduced diarrhea of weaned pigs infected with a pathogenic E. coli.

scholarly journals Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245739
Author(s):  
Inês Vieira da Silva ◽  
Bárbara P. Soares ◽  
Catarina Pimpão ◽  
Rui M. A. Pinto ◽  
Teresa Costa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Biochemical Parameters ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Mrna Levels ◽  
Promising Target ◽  
Dietary Treatments ◽  
Glycerol Permeability

The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation.

scholarly journals Growth performance, nutrient digestibility, and selected fecal microbiota are improved by β-glucan supplementation in weaner pigs

2018 ◽  
Vol 18 (3) ◽  
pp. 769-779
Author(s):  
Xin Jian Lei ◽  
Hyeok Min Yun ◽  
Yi Yang ◽  
In Ho Kim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Growth Performance ◽  
Dry Matter ◽  
Basal Diet ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Coliform Bacteria ◽  
Weaner Pigs ◽  
Blood Profiles

Abstract This study was conducted to evaluate the effects of dietary β-glucan supplementation on growth performance, nutrient digestibility, blood profiles, and fecal characteristics in weaner pigs. A total of 140 weaner pigs [(Yorkshire × Landrace) × Duroc] with an average body weight of 6.37±1.14 kg were allotted to one of the following dietary treatments: 1) negative control (NC, basal diet); 2) positive control (PC, basal diet supplemented with 39 ppm Tiamulin); 3) basal diet supplemented with 0.1% β-glucan (G1); 4) basal diet supplemented with 0.2% β-glucan (G2). During days 22 to 42 and 1 to 42, pigs offered PC and G2 diets grew faster than those offered NC diet (P<0.05). Additionally, during the same periods, pigs fed G2 and PC diets exhibited improved gain:feed ratio compared to pigs receiving NC and G1 diets (P<0.05). Pigs fed G2 diet exhibited higher coefficient of total tract apparent digestibility of dry matter than those fed NC diet (P<0.05). There were no differences on blood profiles, fecal scores, fecal moisture, and fecal pH (P>0.05). Fecal lactic acid bacteria counts in G2 treatment were higher compared with PC and NC treatments (P<0.05). Coliform bacteria concentrations were decreased in pigs fed PC and G2 diets compared with those fed NC diet (P<0.05). In conclusion, the results of the current study indicate that dietary supplementation of 0.2% β-glucan can improve growth performance and dry matter digestibility, increase fecal lactic acid bacteria concentration but decrease fecal coliform bacteria concentration in weaner pigs.

Effects of nucleotide supplementation to corn–soybean meal-based diet on growth performance, fecal microflora, and blood profiles of sows and performance of suckling piglets

2019 ◽  
Vol 99 (4) ◽  
pp. 754-763 ◽  
Author(s):  
Y. Jiao ◽  
S.D. Upadhaya ◽  
I.H. Kim
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Linear Increase ◽  
Fecal Microflora ◽  
Suckling Piglets ◽  
And Performance ◽  
Dietary Treatments ◽  
Piglet Survival ◽  
Blood Profiles

A total of 45 sows (Landrace × Yorkshire) and their litters were used in this study to evaluate the efficacy of nucleotide supplementation on reproductive performance, growth performance, fecal microflora, and blood profiles in sows and piglets. Sows were allocated to one of three treatments (15 pigs per treatment and the average parity was 4.13). Dietary treatments consisted of (1) CON: basal diet, (2) T1: CON + 0.5% nucleotides, and (3) T2: CON + 1.0% nucleotides. In this study, the average daily feed intake during lactation and back fat at weaning day was increased linearly (P < 0.05) in sows with the increase in nucleotide supplementation levels. Sows receiving increasing levels of nucleotides had linearly increased fecal Lactobacillus counts and decreased Escherichia coli counts at weaning day (P < 0.05). Linear reduction of the epinephrine, norepinephrine, and cortisol in sows was observed at weaning day, whereas superoxide dismutase was increased linearly (P < 0.05). Body weight and average daily gain in piglets were linearly (P < 0.05) increased with the increasing levels of nucleotides. Linear increase (P < 0.05) was also observed on total piglets born, live piglets, and piglet survival. The current study demonstrated the importance of nucleotides in the diets of sows to improve sow and piglet performance.

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