Detection of TNF-α as a Cofactor in the Pathogenesis of Nasal Polypi

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohammed Abdelraaof ◽  
Tarek Mostafa Kamal ◽  
Ahmed Mahmoud Maarouf ◽  
Zeinab RezkAbd-elnazer Ali
Keyword(s):  
Risk Factor ◽  
Nasal Polyposis ◽  
Scientific Evidence ◽  
Gene Promoter ◽  
University Hospital ◽  
Inflammatory Factors ◽  
Tnf Alpha ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference

Abstract Background Nasal Polyposis (NP) is a complex multi-factorial disease; associated with several environmental, genetic and inflammatory factors. TNF-alpha is one of the major pro-inflammatory cytokines involved in NP pathogenesis. Some of the polymorphisms of this gene affect its expression. Aim of the Work To evaluate the polymorphism of TNF-alpha G/A308 gene and its association with nasal polyposis in Egypt. Patients and Methods In this case-control study, 25 patients with NP and 25 healthy individuals referred to Ain Shams University hospital were evaluated. After DNA extraction, RFLP-PCR was used to determine polymorphism. Chi-square test was used to compare the frequency distribution of genotype and alleles of TNFalpha gene with NP. The frequency of genotype G/G, A/A and G/A in the NP group was 8, 40 and 52%, and in the control group was 76, 1 and 5%, respectively. Results There was a statistically significant difference between genotype G/G in two groups (P = 0.0001). In addition, the frequency of allele A in patients and controls was 10 and 1%, respectively; and this difference was statistically significant (p = 0.0001). The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for NP in Egypt and the minor frequency of TNF-alpha G308A allele in the current study is slightly more than other major populations. However, more investigations with high number of population are necessary in future. Conclusion According to scientific evidence on TNF-α gene promoter G/A 308 polymorphism in Egypt, it seems that the pattern of genotypic distribution in all areas is the same. However, we found the greater amount of allele A in this study compared with the control group, and the occurrence of G/A genotype related to NP but for more valid results, a larger sample size is necessary. However on our results this polymorphism might be considered as a risk factor of susceptibility of NP in Egyptian people.

FC04-02 - Traumatic experiences as a risk factor for suicide in adolescence

2011 ◽  
Vol 26 (S2) ◽  
pp. 1829-1829
Author(s):  
M. Delalle ◽  
K. Dodig-Ćurković ◽  
P. Filaković
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Early Recognition ◽  
Child And Adolescent Psychiatry ◽  
University Hospital ◽  
Traumatic Experience ◽  
Traumatic Experiences ◽  
Hospital Treatment ◽  
Control Group ◽  
Significant Difference

IntroductionToday, the general interpretation of the etiology of various psychopathological symptoms in adolescence does not talk about causes, but the risk factors.AimWe tried to determine whether traumatic experiences among adolescents represent a risk factor for suicide.MethodsThe study was conducted at the University Department for Child and Adolescent Psychiatry, University Hospital Osijek in 2006. and 2007. years.In the study period we included 100 patients, the experimental group consisted of 50 patients who were admitted to the department for attempted suicide in the order of admission to hospital treatment, the control group consisted of 50 patients admitted to the department for other psychiatric disorders, also in the order of admission to treatment / diagnosed according to DSM IV/.ResultsAge range of 13–18 years. There was no statistically significant difference between the two groups of subjects according to age (χ2 = 5289, df = 5, p = .382) and according to sex (χ2 = .694, df = 1, p = .405). In the suicidal group 38 patients (76%) reported traumatic experiences /most in family context/ while in nonsuicidal group 27 patients (54%) what is statistical difference (χ2 = 5319, df = 1, p = .021).ConclusionIn daily psychiatric work we must focus on adolescents who have experienced traumatic experiences and is therefore extremely important in anamnesis always ask for the lived traumatic experience. Family practitioners, specialists in educational institutions, parents and adolescents themselves have a role in early recognition of these risk factors.

scholarly journals A Randomized Control Trial Study to Determine the Effect of Melatonin on Serum Levels of IL-1β and TNF-α in Patients with Multiple Sclerosis

2020 ◽  
Author(s):  
Masoomeh Yosefifard ◽  
Gholamhassan Vaezi ◽  
Ali Akbar Malekirad ◽  
Fardin Faraji ◽  
Vida Hojati
Keyword(s):  
Multiple Sclerosis ◽  
Interleukin 1 ◽  
Serum Levels ◽  
Inflammatory Factors ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Treatment Groups ◽  
Tnf Α ◽  
Significant Difference ◽  
The Central Nervous System

Multiple sclerosis (MS) is the most common neurological disease that happens at a young age. MS is an inflammatory disease; associated with the demyelination of the central nervous system. Therefore, some inflammatory factors are effective in the mechanism and progression of the disease. Melatonin, as a multi-effect substance including anti-inflammatory effects, can reduce symptoms of MS in patients with a change in their inflammatory factors level. In this study, 50 MS patients who were referred to the MS Society of Markazi Province were randomly selected. All patients were treated with routine MS treatment (interferon) and were divided into control (25 placebo recipients) and treatment (25 recipients of 3 mg melatonin per day for 24 weeks) groups. Anthropometric data of patients including height, weight, and age were determined. Blood samples were collected after fasting in order to determine serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α). Then, samples were immediately centrifuged for serum separation and sera were transferred to a freezer at -80°C and serum levels of these factors were determined; using ELISA kit. The results of this study showed that there was no significant difference between the control and treatment groups in terms of serum levels of TNF-α. However, the level of IL-1β was significantly reduced in the treatment group compared to the control group, indicating that melatonin decreases this inflammatory substance. Our findings suggest a valuable strategy in the treatment of patients who suffer from MS

scholarly journals Protection of Ang1-7 througth MKK/P38MAPKs inflammatory signal pathway on TNF-α stimulated mouse HL-1 cells

2021 ◽  
Author(s):  
Xuewen Wang ◽  
Yuan Cao ◽  
Pradeep depark ◽  
Deepark Sharma ◽  
Guangping Li ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signal Pathway ◽  
Inflammatory Factors ◽  
Control Group ◽  
Inflammatory Factor ◽  
Tnf Α ◽  
Significant Difference ◽  
Atrial Myocyte ◽  
Group B ◽  
Group Control Group

Abstract Background to explore the effect of Ang1-7 througth MKK/P38MAPKs inflammatory signaling pathway on TNF-α-stimulated mouse HL-1 cells. Methods Using TNF-α (100 µg/ml) to establish an inflammatory atrial fibrillation model in HL-1 cell, which derived from mouse atrial myocyte. treated HL-1 cells with different concentrations of Ang 1-7 (0.1, 1 and 10 mmol/L) and divided into 5 groups, namely A group(control group), B group(TNF ), C group(TNF + Ang 1-7 0.1 mmol/L), D group(TNF + Ang 1-7 1 mmol/L ) and E group(TNF + Ang 1-7 10 mmol/L ). Firstly, different concentrations of Ang 1-7 (0.1 mmol/L, 1 mmol/L and 10 mmol/L) were used to stimulate for half an hour, and then TNF-α (100 µg/ml) was added to stimulate for four hours. Both the cells and supernatant were collected. Cells were collected for Western Blotting to detect the protein expression of MKK3, MKK4, MKK6, PMMK4 and PP38. The supernatant was subjected to flow cytometry for detecting multi-inflammatory factors. Results Compared with the A group, the protein expression of MKK3, MKK4, MKK6, PMMK4 and PP38 was statistically significant increased after stimulation with inflammatory factors (TNF-α) (P < 0.05). After intervention with Ang 1-7, the protein expression of MKK3, MKK4, MKK6, PMMK4 and PP38 was statistically significant lower than that of B group (P < 0.05). There is no significant difference of the protein expression of P38 after stimulation with inflammatory factor (TNF-α). Compared with the A group, there was no significant difference in the protein expression of MAS after the stimulation of inflammatory factor (TNF-α). After the intervention of Ang 1-7, the protein expression of MAS was higher than that of the A group and B group, but there was no significant difference (P > 0.05). The expression of MAS protein had an increasing trend, but there was no significant difference (P > 0.05). TGF-β, TNF-α was significantly increased after stimulating factor (TNF-α) was given, but was decreased after the intervention of Ang 1-7, both there were statistically significant (P < 0.05). IL-6 also had the same trend, but there was no significant difference. Conclusion Ang1-7 througth MKK/P38MAPKs inflammatory signal pathway protected on TNF-α stimulated mouse HL-1 cells

scholarly journals Effect of arthroscopic surgery combined with platelet-rich plasma in the treatment of discoid meniscus injury of knee joint and its influence on serum inflammatory factors

2019 ◽  
Vol 17 ◽  
pp. 205873921881433
Author(s):  
Jia Liu
Keyword(s):  
Knee Joint ◽  
Platelet Rich Plasma ◽  
Arthroscopic Surgery ◽  
Inflammatory Factors ◽  
Control Group ◽  
Study Group ◽  
Discoid Meniscus ◽  
Meniscus Injury ◽  
Tnf Α ◽  
Significant Difference

This study was to observe the effect of arthroscopic surgery combined with platelet-rich plasma (PRP) in the treatment of discoid meniscus injury of knee joint and its influence on serum inflammatory factors. A total of 80 patients with discoid meniscus injury in our hospital from June 2014 to June 2016 were enrolled and divided into study group and control group randomly with 40 patients in each group. The control group was treated with arthroscopy alone, and the study group was treated with arthroscopy combined with PRP. The clinical efficacy of the two groups was observed, and the Lysholm scores, Knee injury and Osteoarthritis Outcome Score (KOOS scores), and serum inflammatory factors (IL-1, TNF-α, and IL-6) were compared between the two groups before and after treatment. Six months after the treatment, the improvement rate, defined as (Excellent + Good)/total*100, of the study group was 97.5%, significantly higher than that of the control group, which was 87.5% ( P < 0.05); before treatment, there was no significant difference in both Lysholm scores and KOOS scores between the two groups ( P > 0.05); and 6 months later, the Lysholm scores and KOOS scores in the two groups were both significantly higher than those before, and the scores in the study group increased more significantly compared with those of the control group ( P < 0.05); the values of serum IL-1, TNF-α, and IL-6 in the two groups were both significantly lower than those before, and the values in the study group decreased more significantly compared with those of the control group ( P < 0.05). In conclusion, arthroscopic surgery combined with PRP therapy can effectively improve the clinical symptoms and reduce the level of serum inflammatory factors in patients with discoid meniscus injury of the knee, which is worthy of promotion and application.

scholarly journals PO-241 Effects of Aerobic Exercise on Activation of Neuroglia Cell and Expression of Inflammatory Factors in Aging Rats

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Yaya Xiong ◽  
Jia Jia ◽  
Xuanming Hao
Keyword(s):  
Aerobic Exercise ◽  
Exercise Intervention ◽  
Exercise Group ◽  
Inflammatory Factors ◽  
Control Group ◽  
Sprague Dawley ◽  
Tnf Α ◽  
Significant Difference ◽  
Group A ◽  
Aging Models

Objective In recent years, aerobic exercise has been considered as a method of anti-aging. The aging mechanism in nervous system is closely related to increased activation of neuroglia cells and progressing neuroinflammation with aging.This study tried to shed some light on relationship between chronic inflammation accumulation and aerobic exercise so as to understand how exercise intervened nervous aging through inflammation. In this study, we used D- galactose aging models, applying two intervention ways: aerobic exercise during aging process, after that, to explore the changes of astrocytes, microglia and TNF-α expression. This study aims to provide certain evidence that sport plays a role in anti-aging. Methods Fifty male Sprague-Dawley rats at age of eight weeks, are randomly divided into five groups: control group(A), Aging group(S), Aging while exercising group(YS), After aging Quiet group(SA), After aging exercise group(SY). The aging groups were subcutaneously injected with D- galactose 150mg / kg / d for six weeks. Group YS do swimming exercise three times a week,once 60 minutes in the first six weeks. SY had same exercise intervention in the 7-12 week after injection. We took the hippocampus of rats in A, S, YS at the end of the sixth week, and of those in SA, SY at the end of twelfth week. tested the expression of GFAP, CD11b and TNF-α by immunohistochemical staining. Results 1.GFAP immunohistochemistry: Compared with group A,The expression of GFAP in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of GFAP in group YS significantly decreased by 20.5% (P<0.01). There is no significant difference between group SA and SY(P > 0.05). 2.CD11b immunohistochemistry: Compared with group A, The expression of CD11b in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of CD11b in group YS decreased by 18.4% (P < 0.05).There is no significant difference between group SA and SY(P > 0.05). 3.TNF-α Immunohistochemistry: Compared with group A,The expression of TNF-α in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of TNF-α in group YS decreased by 30.1% (P < 0.01).There is no significant difference between group SA and SY(P > 0.05). Conclusions Exercise could effectively delay aging progression through improving neuroinflammation, but hard to reverse it, so the earlier in age to exercise, the better to delay aging.  

Effect of Resveratrol on NF-κB Activity in Rat Peritoneal Macrophages

2006 ◽  
Vol 34 (04) ◽  
pp. 623-630 ◽  
Author(s):  
Zhen-Hua Ma ◽  
Qing-Yong Ma ◽  
Lian-Cai Wang ◽  
Huan-Chen Sha ◽  
Sheng-Li Wu ◽  
...  
Keyword(s):  
Culture Medium ◽  
Interleukin 1 ◽  
Peritoneal Macrophages ◽  
Inflammatory Factors ◽  
Future Application ◽  
Control Group ◽  
Sprague Dawley ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Significant Difference

This study was to investigate the inhibitive effect of resveratrol (RESV) on nuclear factor kappa B (NF-κB) expression and activity induced by lipopolysaccharide (LPS) in rat peritoneal macrophages (PMA). Male Sprague-Dawley (SD) rats were randomly divided into 7 groups, including control group, LPS group and RESV I-V group. In the LPS group, PMA were incubated in DMEM containing LPS (10 μg/ml), whereas in control group, PMA were incubated in DMEM only. In the RESV I-V groups, PMA were incubated in DMEM containing LPS (10 μg/ml) and different concentrations of RESV. After 24 hours of incubation, NF-κB activity in PMA, and the levels of tumor necrosis factor alpha (TNF-α), interleukin-1 (IL-1) and nitric oxide (NO) in the culture medium were measured. In the concentrations of 1.25-5 μg/ml, RESV had a dose- dependent inhibitive effect on NF-κB activity in PMA as well as the expressions of TNF-α, IL-1 and NO in the culture medium contrasted with the LPS group. There was no significant difference in the levels of these pro-inflammatory factors between the groups of 5 μg/ml and 10 μg/ml RESV. In conclusion, RESV has the potential for the future application of preventing inflammatory diseases involving PMA.

scholarly journals Clinical study of pituitary tumors with EEA resection and its influence on the expression of tumor necrosis factor-α, interleukin-6, and interleukin-12 in patients

2019 ◽  
Vol 17 ◽  
pp. 205873921983704
Author(s):  
Wentao Wang ◽  
Dequan Zhong ◽  
Hua Cheng ◽  
Chengfu Ji ◽  
Zhouming Shen ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Operation Time ◽  
Serum Levels ◽  
Inflammatory Factors ◽  
Interleukin 12 ◽  
Control Group ◽  
Observation Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Factor Α

The aim of this study is to investigate the efficacy of expanded endonasal approaches (EEAs) in the treatment of pituitary adenoma, and the effects of serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-12 (IL-12) in patients were further analyzed. A total of 80 cases of patients with pituitary adenoma admitted to our hospital from January 2013 to May 2015 were randomly divided into the observation group and control group, with 40 cases in each group. The observation group was treated with EEA technique, while the control group was treated with transcranial microsurgery. The prolactin (PRL), growth hormone (GH) and other endocrine hormone indexes, as well as inflammatory factors such as TNF-α, IL-6, and IL-12 were compared before and 1 week after the operation between the two groups. Moreover, the scores of Karnofsky performance status (KPS) and Mini-Mental State Examination (MMSE) at preoperative and postoperative 1 week were also compared between the two groups. The operation time, hospitalization time, and postoperative complications were compared between the two groups. And the patients were followed up for 2 years to observe the recurrence rate. The operation time and hospital stay in the observation group were 62.8 ± 9.3 min and 12.5 ± 2.1 days, respectively, while the operation time and length of stay in the control group were 105.6 ± 15.7 min and 18.2 ± 3.4 days, respectively. The operation time and hospitalization time were shorter than those in the control group, and the differences were statistically significant ( P < 0.05). Before surgery, there was no significant difference in serum levels of PRL and GH between the two groups ( P > 0.05). At 1 week after surgery, the levels of PRL and GH in the two groups were significantly lower than those before surgery ( P < 0.05). And the serum levels of PRL and GH in the observation group were significantly lower than those in the observation group at 1 week after the operation ( P < 0.05). Before operation, there was no significant difference between the two groups of KPS and MMSE scores ( P > 0.05). At 1 week after operation, the scores of KPS and MMSE in both groups were significantly higher than those before the operation ( P < 0.05), and the scores of KPS and MMSE in the observation group were significantly higher than those in the control group ( P < 0.05). Furthermore, there was no significant difference in the serum levels of IL-6, IL-12, and TNF-α between the two groups before operation ( P > 0.05). At 1 week after surgery, the serum levels of IL-6, IL-12, and TNF-α in the two groups were significantly lower than those before surgery ( P < 0.05), while the serum levels of IL-6, IL-12, and TNF-α in the observation group were evidently lower than those in the control group at 1 week after the operation. Besides, the incidence of postoperative complications in the observation group was 7.5%, which was significantly lower than that in the control group (17.5%) ( P < 0.05). All the patients in the two groups were followed up for 2 years. And there was no recurrence in the observation group and one case in the control group. The therapeutic effect of EEAs on pituitary adenoma is better. It can improve the level of high endocrine hormone in patients with pituitary adenoma, improve the functional status of the patients, reduce the serum level of inflammatory factors, and shorten the hospital stay. Meanwhile, it also has the characteristics of fewer complications and low recurrence rate, so it can be popularized in clinical practice.

scholarly journals Correlation Between Tumor Microenvironment and the Inflammatory Factors in Lymphoma-Burdened Mice

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 5133-5133
Author(s):  
Jun Zhang ◽  
Jun Ni ◽  
Pei Xu ◽  
Qingqing Shi ◽  
Jian Gu ◽  
...  
Keyword(s):  
Conflicts Of Interest ◽  
Tumor Therapy ◽  
B Cell Lymphoma ◽  
Inflammatory Factors ◽  
Control Group ◽  
Mice Model ◽  
Buffer Solution ◽  
Tumor Tissues ◽  
Tnf Α ◽  
Significant Difference

Abstract Introduction Since Vichow proposed the hypothesis that chronic inflammation was closely related to tumor in 1863, experimental, clinical and epidemiological studies have shown that inflammation plays an important role in tumor progression, immune response, tumor therapy and prognosis. In this study, 38B9 cells were injected into BALB/c mice to establish lymphoma-burdened mice model. The expression of IL-10, TNF- and IL-6 were observed in plasma and tissues to study the effects of tumor inflammatory factors on tumor environment. Methods Ten 9-week-old BALB/c mice were randomly divided into the control group and the tumor-burdened group. Each mouse in tumor-burdened group was injected with 2×106/200μl 38B9 cells, while the control group was injected with 200μl PBS buffer solution. The establishment of B-cell lymphoma tumor-burdened mice model was confirmed by magnetic resonance information (MRI). The peripheral blood samples were collected before injection, after tumorigenesis and when sacrificed. The tumor tissues were also collected. The level of IL-6, IL-10, TNF-α in the plasm were detected by enzyme-linked immuno sorbent assay (ELISA) and the relative value of mRNA in tissues were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Results 1.Compared with the control group, the levels of IL-6 and TNF-α in the tumor-burdened group were increased after injected 14 days (P <0.05). However, the level of IL-10 showed no significant difference (P >0.05). After injected 19 days, the levels of IL-6, IL-10 and TNF-α of tumor-burdened group were decreased (P<0.05). 2. The expression of IL-10 mRNA in tumor tissues was lower than the control group (P <0.05), while the expression of IL-6 mRNA in borderline tissues was higher than the control group (P<0.05). Compared with tumor tissue, the expression of IL-10 mRNA was increased in borderline tissue (P <0.05). The level of IL-6 and TNF-α showed no significant difference (P>0.05). Conclusions 1. The level of IL-6, IL-10 and TNF-α in the tumor tissues and plasma were significantly higher after tumorigenesis. 2. The increased expression of inflammatory factors is related to tumor-induced inflammatory microenvironment. Disclosures No relevant conflicts of interest to declare.

scholarly journals Effect of Moxibustion on β-EP and Dyn Levels of Pain-Related Indicators in Patients with Rheumatoid Arthritis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yingni Wang ◽  
Siyu Tao ◽  
Zeyun Yu ◽  
Yun Luo ◽  
Yuan Li ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Treatment Group ◽  
Clinical Symptoms ◽  
Inflammatory Factors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Routine Inspection ◽  
Anti Inflammatory

Background. Rheumatoid arthritis (RA) is a systemic immunodeficiency disease characterized by persistent synovial inflammation, pannus formation, and bone and cartilage destruction, resulting in joint malformations and function decline. Objective. The purpose of this study is to evaluate the effect of moxibustion on clinical symptoms and levels of pain-related indicators beta-endorphin (β-EP) and dynorphin (Dyn) in patients with RA and to explore the potential anti-inflammatory and analgesic mechanisms of moxibustion in RA treatment. Methods. A total of 64 patients with RA who met the inclusion criteria were randomly and equally classified into the control and treatment groups. The control group received conventional treatment (oral methotrexate, folate, or leflunomide prescribed for a long time). The treatment group was treated with moxibustion at ST36 (Zusanli), BL23 (Shenshu), and Ashi points with respect to the control group. Patients’ clinical symptoms and routine inspection indexes (rheumatoid factor [RF], erythrocyte sedimentation rate [ESR], and C-reactive protein [CRP]) were recorded before and after treatment. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), β-EP, and Dyn were determined by enzyme-linked immunosorbent assay (ELISA). The software SPSS24.0 was used for statistical analysis. Results. (1) Compared with the pretreatment result, both of the two groups’ clinical symptoms and routine inspection indexes (RF, ESR, and CRP) improved ( P  < 0.05), and the improvement of clinical symptoms in the treatment group outperformed that in the control group ( P  < 0.05). (2) TNF-α and IL-1β levels decreased significantly in the treatment group after treatment ( P  < 0.01), while no significant difference was observed in the control group ( P  > 0.05). (3) β-EP and Dyn levels in the treatment group were significantly increased after treatment ( P  < 0.01, P  < 0.01), but the control group showed no significant difference ( P  > 0.05, P  > 0.05). It is worth mentioning that the serum TNF-α, IL-1β, β-EP, and Dyn levels between the two groups were significantly different after 8 weeks of treatment ( P  < 0.05). (4) Differences in the serum β-EP and Dyn levels in the patients of the treatment group were correlated with TNF-α and IL-1β levels after treatment, and the correlation was mainly negative (r < 0). Conclusion. Moxibustion can improve joint pain in patients with RA using conventional western medicine. One of the mechanisms may affect the serum β-EP and Dyn levels by downregulating the inflammatory factors to play an anti-inflammatory and analgesic role.

Altered composition of high-lipid diet may generate reactive oxygen species by disturbing the balance of antioxidant and free radicals

2020 ◽  
Vol 31 (3) ◽  
Author(s):  
Arnab Banerjee ◽  
Debasmita Das ◽  
Rajarshi Paul ◽  
Sandipan Roy ◽  
Ankita Bhattacharjee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Reactive Oxygen ◽  
Control Group ◽  
Preliminary Evaluation ◽  
Oxygen Species ◽  
High Lipid ◽  
Tnf Α ◽  
High Lipid Diet ◽  
Lipid Diet

AbstractBackgroundIn the present era, obesity is increasing rapidly, and high dietary intake of lipid could be a noteworthy risk factor for the occasion of obesity, as well as nonalcoholic fatty liver disease, which is the independent risk factor for type 2 diabetes and cardiovascular disease. For a long time, high-lipid diet (HLD) in “fast food” is turning into part of our everyday life. So, we were interested in fulfilling the paucity of studies by means of preliminary evaluation of these three alternative doses of HLD on a rat model and elucidating the possible mechanism of these effects and divulging the most alarming dose.MethodsThirty-two rats were taken, and of these, 24 were fed with HLD in three distinctive compositions of edible coconut oil and vanaspati ghee in a ratio of 2:3, 3:2 and 1:1 (n = 8), orally through gavage at a dose of 10 mL/kg body weight for a period of 28 days, whereas the other eight were selected to comprise the control group.ResultsAfter completion of the experiment, followed by analysis of data it was revealed that hyperlipidemia with increased liver and cardiac marker enzymes, are associated with hepatocellular injury and cardiac damage. The data also supported increased proinflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α). As oxidative stress parameter increased in both liver and heart, there is also an increased in TNF-α due to an increased expression of inducible nitric oxide (NO) synthase, which led to a high production of NO. Moreover, HLD treatment explicitly weakens reasonability of hepatocytes and cardiomyocytes conceivably through G0/G1 or S stage capture or perhaps by means of enlistment of sub-G0/G1 DNA fragmentation and a sign of apoptosis.ConclusionsBased on the outcomes, it tends to be inferred that consequences of the present examination uncovered HLD in combination of 2:3 applies most encouraging systemic damage by reactive oxygen species generation and hyperlipidemia and necroapoptosis of the liver and heart. Hence, outcome of this study may help to formulate health care strategy and warns about the food habit in universal population regarding the use of hydrogenated and saturated fats (vanaspati ghee) in diet.

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