scholarly journals QuantifyPolarity, a new tool-kit for measuring planar polarized protein distributions and cell properties in developing tissues

2020 ◽  
Author(s):  
Su Ee Tan ◽  
Weijie Tan ◽  
Katherine H. Fisher ◽  
David Strutt
Keyword(s):  
Quantitative Methods ◽  
Principal Component ◽  
Asymmetric Distribution ◽  
Planar Polarity ◽  
Automated Quantification ◽  
Pca Method ◽  
User Friendly ◽  
Relationship Of ◽  
The Relationship ◽  
Friendly Graphical User Interface

SummaryThe coordination of cells or structures within the plane of a tissue is known as planar polarization. It is often governed by the asymmetric distribution of planar polarity proteins within cells. A number of quantitative methods have been developed to provide a readout of planar polarization of protein distributions. However, the quantification of planar polarization can be affected by different cell geometries. Hence, we developed a novel planar polarity quantification method based on Principal Component Analysis (PCA) that is shape insensitive. Here, we compare this method with other state-of-the-art methods on simulated models and biological datasets. We found that the PCA method performs robustly in quantifying planar polarity independently of variation in cell geometry. Furthermore, we designed a user-friendly graphical user interface called QuantifyPolarity, equipped with three different methods for automated quantification of polarity. QuantifyPolarity also provides image analysis tools to quantify cell morphology and packing geometry, allowing the relationship of these characteristics to planar polarization to be investigated. This all-in-one tool enables experimentalists with no prior computational expertise to perform high-throughput cell polarity and shape analysis automatically and efficiently.

scholarly journals QuantifyPolarity, a new tool-kit for measuring planar polarized protein distributions and cell properties in developing tissues

Development ◽  
2021 ◽  
Author(s):  
Su Ee Tan ◽  
Weijie Tan ◽  
Katherine Fisher ◽  
David Strutt
Keyword(s):  
Quantitative Methods ◽  
Principal Component ◽  
Asymmetric Distribution ◽  
Planar Polarity ◽  
Cell Geometry ◽  
Automated Quantification ◽  
Pca Method ◽  
User Friendly ◽  
Relationship Of ◽  
The Relationship

The coordination of cells or structures within the plane of a tissue is known as planar polarization. It is often governed by the asymmetric distribution of planar polarity proteins within cells. A number of quantitative methods have been developed to provide a readout of planar polarized protein distributions. However, previous planar polarity quantification methods can be affected by variation in cell geometry. Hence, we developed a novel planar polarity quantification method based on Principal Component Analysis (PCA) that is shape insensitive. Here, we compare this method with other state-of-the-art methods on simulated models and biological datasets. We found that the PCA method performs robustly in quantifying planar polarity independently of variation in cell geometry and other image conditions. We designed a user-friendly graphical user interface called QuantifyPolarity, equipped with three polarity methods for automated quantification of polarity. QuantifyPolarity also provides tools to quantify cell morphology and packing geometry, allowing the relationship of these characteristics to planar polarization to be investigated. This tool enables experimentalists with no prior computational expertise to perform high-throughput cell polarity and shape analysis automatically and efficiently.

HUBUNGAN KEPADATAN BIVALVIA DENGAN PARAMETER LINGKUNGAN DI PESISIR TANJUNG PURA KABUPATEN BANGKA TENGAH

2020 ◽  
Vol 13 (2) ◽  
pp. 112-121
Author(s):  
Sudiyar . ◽  
Okto Supratman ◽  
Indra Ambalika Syari
Keyword(s):  
Negative Impact ◽  
Principal Component ◽  
Environmental Parameters ◽  
Data Retrieval ◽  
Distribution Patterns ◽  
Random System ◽  
System Method ◽  
Relationship Of ◽  
The Relationship ◽  
Anadara Granosa

The destructive fishing feared will give a negative impact on the survival of this organism. This study aims to analyze the density of bivalves, distribution patterns, and to analyze the relationship of bivalves with environmental parameters in Tanjung Pura village. This research was conducted in March 2019. The systematic random system method was used for collecting data of bivalves. The collecting Data retrieval divided into five research stasions. The results obtained 6 types of bivalves from 3 families and the total is 115 individuals. The highest bivalve density is 4.56 ind / m², and the lowest bivalves are located at station 2,1.56 ind / m²,  The pattern of bivalve distribution in the Coastal of Tanjung Pura Village is grouping. The results of principal component analysis (PCA) showed that Anadara granosa species was positively correlated with TSS r = 0.890, Dosinia contusa, Anomalocardia squamosa, Mererix meretrix, Placamen isabellina, and Tellinella spengleri were positively correlated with currents r = 0.933.

scholarly journals Correlation of environmental factors and spatial distribution of moluscs communities in mangrove reboisation areas of Seribu Islands, Indonesia

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Syahrial Syahrial ◽  
Eryc Pranata ◽  
Hendri Susilo
Keyword(s):  
Principal Component Analysis ◽  
Spatial Distribution ◽  
Environmental Factors ◽  
Principal Component ◽  
Quality Standard ◽  
Small Plot ◽  
Line Transects ◽  
Relationship Of ◽  
The Relationship ◽  
Mangrove Reforestation

Mangrove reforestation is often carried out in various regions or regions, but information about the relationship of environmental factors and the distribution of fauna associations is still very minimal. The Principal Component Analysis (PCA) study on the correlation of environmental factors and the spatial distribution of the molusks community in the Seribu Islands mangrove reforestation area was conducted in March 2014 with the aim of analyzing environmental factors for the diversity and presence of the molusks. Environmental factors are measured insecurely, while the moluccan community is collected by making line transects and plots measuring 10 x 10 m2 and in the size of 10 x 10 m2, a small plot of 1 x 1 m2 is made. The results of the study show that environmental factors are not so different between stations and do not exceed the quality standard for the lives of 4 species of mollusks, where the parameters of aquatic pH are the environmental factors that most influence their distribution.Keywords: environmental factors, distribution, mollusks community, mangrove reforestation, Seribu Islands

scholarly journals The Influence of the Application of Marketing Mix on the Purchase Decision of Prospective Consumers of Grand Cempaka Housing

2020 ◽  
Author(s):  
Gesang Sudrajad ◽  
J.E Sutanto
Keyword(s):  
Regression Analysis ◽  
Data Analysis ◽  
Quantitative Methods ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Marketing Mix ◽  
Marketing Strategies ◽  
Purchase Decision ◽  
Relationship Of ◽  
The Relationship

The purpose of this study was to examine the effect of simultaneous and partial marketing mix on the purchase decision of prospective consumers of Grand Cempaka Housing.This research uses quantitative methods, and is a survey research from the types of explanatory research whose focus is placed on relational research: studying the relationship of variables. The object of research is the residents of Pasuruan City and the sample in this study is the Prospective Consumer of Grand Cempaka Housing. Data analysis uses multiple linear regression analysis methods.The results of the analysis show that Products, Prices, Places and Promotions simultaneously have a significant effect on the Decision of Purchasing Prospective Consumers of Grand Cempaka Housing. This shows that the marketing mix is one of the marketing strategies that should be considered so that the implementation of the marketing strategy can run successfully because the elements in the marketing mix influence each other. Variables in the marketing mix which include Products, Prices, Places and Promotions partially have a significant effect on the Decision of Purchasing Prospective Consumers of Grand Cempaka Housing. Keywords: marketing mix; purchase decision; property; housing

scholarly journals A Differential Host Response to Viral Infection Defines a Subset of Earlier-Onset Diverticulitis Patients

2018 ◽  
Vol 27 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Kathleen M Schieffer ◽  
Bryan P Kline ◽  
Leonard R Harris ◽  
Sue Deiling ◽  
Walter A Koltun ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Principal Component ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Viral Pathogen ◽  
Viral Response ◽  
Elevated Expression ◽  
Bioinformatic Approaches ◽  
Relationship Of ◽  
The Relationship

Background & Aims: Diverticulitis is the chronic inflammation of diverticula. Whether the pathophysiology of earlier-onset patients differs from later-onset patients is unknown. We profiled the colonic transcriptomes of these two patient populations to gain insight into the molecular underpinnings of diverticulitis. Methods: We conducted deep RNA sequencing (RNA-seq) on colonic segments surgically resected from earlier-onset (<42 years old, n=13) and later-onset (>65 years old, n=13) diverticulitis patients. We used bioinformatic approaches to cluster the patients based on the relationship of differentially expressed genes and to inform on the molecular pathways that segregated the clusters. Results: Principal component analysis identified three patient clusters; diverticulitis later-onset (DVT-LO), diverticulitis mixed-onset (DVT-MO), and diverticulitis earlier-onset (DVT-EO). The patients comprising DVT-EO, which was the majority of earlier-onset patients, displayed increased expression of anti-viral response genes. This finding was confirmed using an independent weighted co-expression network analysis (WGCNA) of differentially expressed genes. Conclusions: We found that the majority of patients with earlier-onset disease contained elevated expression of host genes involved in the anti-viral response. Thus, susceptibility to a viral pathogen may offer one explanation why some individuals develop diverticulitis at an earlier age.

scholarly journals Tingkat Kesesuaian Gaya Kepemimpinan Ketua dengan Kematangan Anggota Hubungannya dengan Partisipasi Anggota

TRIKONOMIKA ◽  
2013 ◽  
Vol 12 (2) ◽  
pp. 145
Author(s):  
Abd. Rahman Pakaya
Keyword(s):  
Statistical Analysis ◽  
Leadership Styles ◽  
Leadership Style ◽  
Quantitative Methods ◽  
Hypothesis Test ◽  
Maturity Level ◽  
Analysis Techniques ◽  
Task Behavior ◽  
Relationship Of ◽  
The Relationship

The purpose of this research is to know the leadership style’s compatibility level which including task behavior  and relationship behaviors, the maturity of the members and the relationship with the  with the participation of members of the Koperasi Unit Desa (KUD) in Gorontalo province. This study use 52 sample of KUD chairman and 140 sample members of KUD.  The calculation of the sample used proportional sampling. Data were collected by interviews and distributing questionnaires to respondent. Method of anayisis using descriptive qualitative analysis techniques and quantitative methods. Hypothesis test using Kruskal Wallis different test and the Chi Quadrat. Based on the statistical analysis results can be concluded: (1) the existence of the leadership styles differentiation of the chairman, (2) there are differences in maturity level of members, (3)  there is no leadership styles compatibility level of chairman toward the maturity of members in KUD at Gorontalo Province, and (4) there is a relationship of the chairman leadership style with the maturity of the members indicated from the participation of members.

scholarly journals The Relationship of Farmers Characteristics and Perception of the Occupational Health and Safety (OHS) Application in Farming Activities in Wonosalam District, Jombang Regency, East Java

2019 ◽  
Vol 7 (2) ◽  
pp. 153-158
Author(s):  
Abdul Farid ◽  
Arum Pratiwi ◽  
Aan Dwi Auliya Fitri
Keyword(s):  
Quantitative Methods ◽  
Occupational Safety ◽  
Health And Safety ◽  
Primary Data ◽  
Land Area ◽  
Level Of Education ◽  
Safety And Health ◽  
Relationship Of ◽  
The Relationship ◽  
And Training

The majority of residents of Wonosalam Subdistrict are farmers who are at high risk of having health problems due to interactions with hazardous materials, such as pesticides. The implementation of occupational safety and health (K3) which is still low triggers the occurrence of accidents and diseases in farmers that have an impact on performance degradation so that it can cause losses. The aim of this study to find out the relationship between farmers 'characteristics and farmers' perceptions in the application of K3 in Wonosalam District, Jombang Regency by using descriptive quantitative methods. Observation variables include age, level of education, training, length of farming, income, land status and land area on perceptions of OHS application. The data used are primary data obtained from 98 farmers using a questionnaire. Analysis of the data used in this study is thecorrelation analysis Spearman Rank. The results showed that the characteristics of farmers associated with farmers' perceptions in applying OSH were the level of education and training. While the characteristics of farmers who are not related to the perception of farmers in the application of OSH are age, length of farming, income, land status and land area.

scholarly journals The Correlation Between the Arm Muscle Strength to Passing Skill in Handball Games

MAENPO ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 194
Author(s):  
Firdaus Hendry Prabowo Yudho ◽  
Muhammad Alvin Nur El Hakim ◽  
Dhika Bayu Mahardhika ◽  
Rahmat Iqbal ◽  
Setio Nugroho ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Linear Regression ◽  
Quantitative Methods ◽  
Simple Linear Regression ◽  
Sample Number ◽  
Normality Test ◽  
Passing Ability ◽  
Arm Strength ◽  
Relationship Of ◽  
The Relationship

This research aims to find out the relationship of arm muscle strength to passing skills in handball games in members of SME (Student Activity Unit) Suryakancana Cianjur University. The method used in this study used quantitative methods of this type of method with a sample number of 30 people. Data collection uses a push pull dynamometer to then perform data normality tests, data correlation tests, and simple linear regression tests. The results of the study on the data normality test obtained significant values of variableS X, 390 and variable Y of ,257 both values showed that the value exceeded a significant level of >0.05 then the data was normal distribution. Based on the results of the correlation test there is a significance value of 0.09 indicating that the data has a relationship. The results of a simple linear regression test obtained an r-square value of 0.323 that there was an influence on the relationship of arm muscle strength to the passing ability in handball games by 0.323%.Key words: Arm strength, Passing ability, Handball, Sports Skill, Motor ability

scholarly journals The Influence of Training and Motivation on the Performance of Business Actors in Busmetik with Competence as an Intervening Variable

2021 ◽  
Vol 9 ◽  
Author(s):  
Diana Kartika Pramodha Wardhani ◽  
Sigit Hermawan
Keyword(s):  
Quantitative Methods ◽  
Business People ◽  
Hr Management ◽  
Competence Training ◽  
Competence Motivation ◽  
Training Motivation ◽  
And Performance ◽  
Relationship Of ◽  
The Relationship ◽  
Production Target

Research Objectives to analyze the effect of Training and Motivation on the performance of Busmetik business people with competence as intervening variabell. Training for cosmetic business actors has been routinely followed, however, the shrimp production target is still not optimally achieved. Quantitative methods are used in this study to prove the hypothesis regarding the relationship of research variables namely Training, Motivation, Competence and Performance. The results of the analysis show that training has a significant effect on competence, motivation has a significant effect on competence, training has a significant effect on performance, motivation has a significant effect on performance, competence has a significant effect on performance. The path analysis results show that competency is able to be an intervening variable for training and motivation for performance. Evident from  the  indirect  influence  of  training  and  motivation on  performance is  greater  than  the  direct  influence. Furthermore, for the Fisheries Office of Pasuruan Regency as a coach, it is necessary to pay attention to Training, Motivation and Competence in HR management because it affects performance directly or indirectly.

scholarly journals Density and characteristics of Green mussels (Perna viridis) in Percut Sei Tuan coastal, North Sumatra, Indonesia

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
IPANNA ENGGAR SUSETYA ◽  
Mohammad Basyuni ◽  
DESRITA DESRITA ◽  
ARIDA SUSILOWATI ◽  
TADASHI KAJITA
Keyword(s):  
Frequency Distribution ◽  
Principal Component ◽  
Perna Viridis ◽  
Resource Potential ◽  
Pattern Frequency ◽  
Physical Chemical ◽  
Bottom Waters ◽  
Relationship Of ◽  
The Relationship ◽  
North Sumatra

Percut Sei Tuan is a coastal area on the east coast of North Sumatra which has a lot of natural resource potential, one of them which is green mussels (Perna viridis). Green mussels have many benefits, namely for consumption, cultivation and bioindicator. This study aimed to determine the density, habitat location, growth pattern, frequency distribution of green mussels, physical-chemical parameters of water, a substrate of the bottom waters, and the relationship of green clams density to water and substrate parameters. The study was conducted in July-September 2020. Data analysis included density, the relationship of total length and weight of green mussels, the frequency distribution of shell lengths, and Principal Component Analysis (PCA). The results showed that the density of green mussels at stations I, II, and III were 352 ind/m2, 3 ind/m2, and 1 ind/m2, respectively. The relationship between the length and weight of green shells shows that the growth was negative allometric. The water condition, seen from the physical, chemical, and substrate parameters, was still suitable for P. viridis. Water and substrate parameters that were positively related to the density of green mussels were water temperature, nitrate, phosphate, C-organic, percentage of silt and clay. Parameters that were negatively related were salinity, pH, and percentage of sand in the bottom substrate. The present study suggested that Station I was an appropriate area for the growth and reproduction of green mussels.

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