Acute Effects of Soccer Training on White Blood Cell Count in Elite Female Players

2007 ◽  
Vol 2 (3) ◽  
pp. 239-249 ◽  
Author(s):  
Alexandra A. Avloniti ◽  
Helen T. Douda ◽  
Savvas P. Tokmakidis ◽  
Alexandros H. Kortsaris ◽  
Evropi G. Papadopoulou ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Training Session ◽  
Exercise Session ◽  
Maximal Heart Rate ◽  
Blood Samples ◽  
National Team ◽  
Significant Difference ◽  
Single Bout ◽  
Soccer Training

Purpose:To investigate the acute changes in leukocyte number and cortisol after a single bout of soccer training.Methods:Ten elite female national-team soccer players and 8 nonathletes participated in the study. The duration of the exercise was 2 h, and it was performed at an intensity of 75% of maximal heart rate (HRmax). Blood samples were taken before, immediately after, and 4 h after a soccer training session to determine total white blood cells; the subsets of neutrophils, lymphocytes, monocytes, eosinophils, and basophils; and cortisol. At the same time, blood samples were obtained from nonathletes who refrained from exercise.Results:Data analysis indicated a significant increase in total white blood cells in the athletes postexercise (P < .001). The leukocytosis was still evident after 4 h of recovery (78% higher than the preexercise values), and there was a significant difference between athletes and nonathletes (P < .001). This leukocytosis was primarily caused by neutrophilia—there were no significant differences in lymphocytes after the end of exercise or between the 2 groups (P > 0.05). In addition, there was a statistically significant difference in cortisol concentration between athletes and nonathletes after the exercise (P < .001).Conclusion:These findings revealed that the single bout of soccer training at an intensity of 75% of HRmax induced leukocytosis without affecting the lymphocyte count in elite female athletes and probably the effectiveness of cellular components of adaptive immunity. Coaches should provide adequate time (>4 h) until the next exercise session.

scholarly journals Influence of Omega-3 in Standardbred Horse: Haematological Parameters

2016 ◽  
Vol 16 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Vincenzo Monteverde ◽  
Fulvio Congiu ◽  
Irena Vazzana ◽  
Salvatore Dara ◽  
Sergio Guttadauro ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Dietary Supplementation ◽  
Haematological Parameters ◽  
Control Group ◽  
Omega 3 ◽  
Blood Samples ◽  
Significant Difference ◽  
Athletic Horse ◽  
Experimental Group

Abstract The aim of this study was to investigate the effect of omega-3 supplementation on some haematological parameters like red blood cells (RBC), haemoglobin (Hgb), haematocrit (Hct), white blood cells (WBC), neutrophils (Neu) and lymphocytes (Lym) that may have a direct effect on the performance of athletic horses. Ten regularly trained Standardbred horses (6 geldings and 4 females, 4-5 years old, mean body weight 500 ± 25 kg) were used for the study. They were randomly divided into two groups. The experimental group received an omega-3 dietary supplementation every day for 30 days. The control group received no supplementation. Every 10 days, horses took part in a 1660 metre harness race and blood samples were collected from each horse: one in the morning before race (pre) and one after race (post). The application of one-way analysis of variance for repeated mesures (ANOVA) showed a statistically significant difference due to the race in the two different groups. The results obtained in the present study show a discordant effect of supplementation with omega-3 on RBC, HCT and Hgb, while omega-3 supplementation has been shown to have a better effect on WBC, Neu and Lym, but further studies should be performed to better evaluate the benefits of these on the performance of the athletic horse.

scholarly journals The effects of acute moderate intensity training on hematological parameters in elite para-badminton athletes

2021 ◽  
pp. 1
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Training Session ◽  
Blood Parameters ◽  
Moderate Intensity ◽  
National Team ◽  
Study Results ◽  
Before And After

Background and objective: The knowledge on the effects of para-badminton sport on physiological parameters is relatively limited in the literature. This study aims to examine the effects of moderate-intensity badminton training on the hematological parameters of elite para-badminton athletes. Methods: A total of 12 para-badminton athletes, 6 female and 6 male, with international competitive experience in the Turkish Para-Badminton National Team, participated in the study. A 90-minute badminton training was applied to the athletes for their technical and tactical development. A 4 mL blood sample was taken before and after the training. Hematological parameters were analyzed from these blood samples. White blood cells, red blood cells, platelets count, and their particular subgroups such as hemoglobin, hematocrit, granulocytes, and lymphocytes were examined as the hematological parameters in this study. Results: The findings revealed that the female para-badminton athletes exhibited a significant increase in white blood cells, granulocytes percentage, red blood cells, hemoglobin, hematocrit, platelets values after training compared to pre-training. It was determined that there were significant increases in white blood cells, granulocytes, granulocytes percentage, red blood cells, and hematocrit values for male para-badminton athletes after training compared to pre-training, and significant decreases in lymphocytes percentage values. Conclusion: In conclusion, the present study showed that a single 90-minute moderate-intensity badminton training session leads to physiological changes on the blood parameters of elite para-badminton athletes. These changes, which differ by gender, can affect the health and performance of the athlete.

scholarly journals Protein O-GlcNAc Modification Increases in White Blood Cells After a Single Bout of Physical Exercise

2018 ◽  
Vol 9 ◽  
Author(s):  
Tamás Nagy ◽  
Emese Kátai ◽  
Viktória Fisi ◽  
Tamás Tibor Takács ◽  
Antal Stréda ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Blood Cells ◽  
White Blood Cells ◽  
Single Bout

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

scholarly journals Profile of cortisol, glycaemia, and blood parameters of American Bullfrog tadpoles Lithobates catesbeianus exposed to density and hypoxia stressors

2012 ◽  
Vol 32 (suppl 1) ◽  
pp. 91-98 ◽  
Author(s):  
Patricia C. Teixeira ◽  
Danielle C. Dias ◽  
Guilherme C. Rocha ◽  
Antônio M. Antonucci ◽  
Fernanda M. França ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Experimental Period ◽  
Blood Parameters ◽  
Air Exposure ◽  
Physiological Profile ◽  
Lithobates Catesbeianus ◽  
American Bullfrog ◽  
Physiological Alterations ◽  
Significant Difference

The aim of this study was to evaluate alterations to the physiological profile (cortisol, glycaemia, and blood parameters) of Lithobates catesbeianus caused by the stressors density and hypoxia. The organisms were in the prometamorphosis stage and exposed to different tadpole densities: 1 tadpole/L (T1), 5 tadpoles/L (T2), and 10 tadpoles/L (T3) for 12 days. The blood was collected through the rupture of the caudal blood vessel and collected under normoxia (immediate collection) and hypoxia (after 15 minutes of air exposure) conditions. Cortisol levels rose on the fourth and eighth days of treatment and returned to basal levels by the end of the experiment. The stressor mechanisms tested did not affect glycaemia. White blood cells (total number of lymphocytes, neutrophils, and eosinophils) showed a significant difference at the twelfth day of the experiment when compared with the start of the experiment. We concluded that, under controlled conditions, a density of up to 10 tadpoles/L and air exposure for 15 minutes did not cause harmful physiological alterations during the experimental period. The answer to these stressors maybe was in another hormonal level (corticosterone).

scholarly journals Cytological analysis of the lung material of C57BL/6Y mice in the simulation of acute respiratory distress syndrome

Biomeditsina ◽  
2021 ◽  
Vol 17 (3E) ◽  
pp. 17-22
Author(s):  
O. V. Alimkina ◽  
A. E. Petrenko
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Bronchoalveolar Lavage ◽  
Blood Cells ◽  
Distress Syndrome ◽  
White Blood Cells ◽  
Intact Group ◽  
Significant Difference ◽  
Over Time

The work is devoted to the study of changes in the cellular composition of bronchoalveolar lavage over time in the modeling of acute respiratory distress syndrome (ARDS) in mice. ARDS was modeled by administering α-galactosylceramide and a mixture of lipopolysaccharide with a complete Freud’s adjuvant. After euthanasia, bronchoalveolar lavage was taken for analysis. On this basis, changes in the total number of white blood cells, the percentage of neutrophils and macrophages were assessed. It was found that the percentage of neutrophils in the ARDS group shows a statistically significant difference from that in the intact group, starting from 3 hours after modeling ARDS. Further, a statistically significant decrease in macrophages was observed. 

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

Direct freezing of whole blood enables analysis of leucocyte markers by flow cytometry: a proof-of-concept study

2021 ◽  
Author(s):  
Pénélope Bourgoin ◽  
Inès Ait Belkacem ◽  
Isabelle Arnoux ◽  
Pierre-Emmanuel Morange ◽  
Fabrice Malergue
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Proof Of Concept ◽  
Fresh Blood ◽  
Blood Samples ◽  
Step Flow ◽  
Freezing Method ◽  
One Step

Aim: A new one-step flow cytometry procedure has been recently demonstrated for identifying subjects with infections, but only for fresh whole blood samples. The goal of this study was to assess its applicability on frozen samples, by proposing a new method to perform the sample freezing directly and easily. Methods: Fresh blood was tested, then frozen either directly or with dimethylsulfoxide and serum. Common markers of white blood cells as well as infection-related biomarkers were tested. Results: All percentages of leucocyte subsets and levels of infection-related biomarkers were significantly correlated between frozen and fresh samples. Conclusion: The direct freezing method enables an accurate assessment of common cellular sub-populations and of levels of important infectious biomarkers via flow cytometry.

Mood Alterations in Older Adults following Acute Exercise

1994 ◽  
Vol 79 (1) ◽  
pp. 191-194 ◽  
Author(s):  
Edgar F. Pierce ◽  
Donald W. Pate
Keyword(s):  
Older Adults ◽  
Repeated Measures ◽  
Acute Exercise ◽  
Mood States ◽  
Exercise Session ◽  
Control Group ◽  
Older Individuals ◽  
Significant Difference ◽  
Single Bout ◽  
Affective Change

Limited research indicates positive affective change following acute bouts of exercise, but whether this improved affect among younger subjects may be generalized to older individuals is not known. The present study, then, examined the effects of a single bout of physical activity among older participants. 16 trained women ( Moge = 64.5 ± 7.6 yr.) completed an abbreviated Profile of Mood States prior to and immediately following a 75-min. session of aerobic line dancing. A series of one-way analyses of variance with repeated measures were used to examine differences between pre- and posttest subscores on mood states. Significant decreases following exercise in scores on Tension, Depression, Fatigue, and Anger and a significant increase in scores on Vigor relative to preexercise (control) scores were found. Global mood was significantly improved after the exercise session. No significant difference was found between pre- and postexercise measures of Confusion. Previous findings of significant improvements in affect immediately after an acute bout of exercise may be generalized to older adults. Repetition with a nonexercised control group is desirable.

scholarly journals Strength Training Session Induces Important Changes on Physiological, Immunological, and Inflammatory Biomarkers

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Ayla Karine Fortunato ◽  
Washington Martins Pontes ◽  
Débora Maria Soares De Souza ◽  
Jéssica Santos Ferreira Prazeres ◽  
Lucas Soares Marcucci-Barbosa ◽  
...  
Keyword(s):  
Strength Training ◽  
Perceived Exertion ◽  
Inflammatory Responses ◽  
White Blood Cells ◽  
Training Session ◽  
Rating Of Perceived Exertion ◽  
Exercise Session ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Muscle Actions

Strength exercise is a strategy applied in sports and physical training processes. It may induce skeletal muscle hypertrophy. The hypertrophy is dependent on the eccentric muscle actions and on the inflammatory response. Here, we evaluate the physiological, immunological, and inflammatory responses induced by a session of strength training with a focus on predominance of the eccentric muscle actions. Twenty volunteers were separated into two groups: the untrained group (UTG) and the trained group (TG). Both groups hold 4 sets of leg press, knee extensor, and leg curl at 65% of personal one-repetition maximum (1RM), 90 s of recovery, and 2″conc/3″eccen of duration of execution in each repetition. Blood samples were collected immediately before and after, 2 hours after, and 24 h after the end of the exercise session. The single session of strength training elevated the heart rate (HR), rating of perceived exertion (RPE), visual analog scale (VAS), and lactate blood level in UTG and TG. Creatine kinase (CK) levels were higher at 2 and 24 h after the end of the exercise in UTG and, in TG, only at 24 h. The number of white blood cells (WBC) and neutrophils increased in UTG and TG, post and 2 h after exercise. Lymphocytes increased postexercise but reduced 2 h after exercise in both groups, while the number of monocytes increased only immediately after the exercise session in UTG and TG. The strength training session elevated the levels of apelin and fatty acid-binding proteins-3 (FABP3) in both groups and brain-derived neurotrophic factor (BDNF) in TG. The single exercise session was capable of inducing elevated HR, RPE, lactate level, and CK levels. This protocol changed the count/total number of circulating immune cells in both groups (UTG and TG) and also increased the level of plasmatic apelin, BDNF, and FLTS1 only in TG and FABP3 myokines in both groups.

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