Studies on antimicrobial activity of cobalt(III) ethylenediamine complexes

2006 ◽  
Vol 52 (12) ◽  
pp. 1247-1254 ◽  
Author(s):  
Penumaka Nagababu ◽  
J Naveena Lavanya Latha ◽  
P Pallavi ◽  
S Harish ◽  
S Satyanarayana
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Mixed Ligand ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
H Nmr ◽  
Time Period ◽  
Infrared Spectral ◽  
Diffusion Assay

A series of cobalt(III) mixed ligand complexes of type [Co(en)2L]+3, where L is bipyridine, 1,10-phenanthroline, imidazole, methylimidazole, ethyleimidazole, dimethylimidazole, urea, thiourea, acetamide, thioacetamide, semicarbazide, thiosemicarbazide, or pyrazole, have been isolated and characterized. The structural elucidation of these complexes has been explored by using absorption, infrared, and 1H NMR nuclear magnetic resonance spectral methods. The infrared spectral data of all these complexes exhibit a band at 1450/cm and 1560–1590/cm, which correspond to C = C and C = N, a band at 575/cm for Co-N (en), and a band at 480/cm for Co-L (ligand). All these complexes were found to be potent antimicrobial agents. The antibacterial activity was studied in detail in terms of zone inhibition, minimum bactericidal, and time period of lethal action. Among all, complexes bipyridine, 1,10-phenanthroline, dimethylimidazole, and pyrazole, possess the highest antibacterial activity. Antifungal activity was done by disc-diffusion assay and 50% inhibitory concentrations that possess high antifungal activity.Key words: cobalt(III) complexes, ethylenediamine, antimicrobial, antifungal.

scholarly journals Enaminone-Derived Pyrazoles with Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Mashooq Ahmad Bhat ◽  
Mohamed A. Al-Omar ◽  
Ahmed M. Naglah ◽  
Abdul Arif Khan
Keyword(s):  
Antimicrobial Activity ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Initial Screening ◽  
Gram Positive ◽  
Gram Negative ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Diffusion Assay

A series of pyrazoles derived from the substituted enaminones were synthesized and were evaluated for antimicrobial activity. All the compounds were characterized by the spectral data and elemental analysis. The synthesized compounds were initially screened for their antimicrobial activity against ATCC 6538, NCTC 10400, NCTC 10418, and ATCC 27853. During initial screening, compounds (P1, P6, and P11) presented significant antimicrobial activity through disc diffusion assay. These compounds were further evaluated for antimicrobial activity at different time points against Gram-positive and Gram-negative bacteria and presented significant activity for 6 hours. The activity was found to be greater against Gram-positive bacteria. In contrast at 24 hours, the activity was found only against Gram-positive bacteria except compound (P11), showing activity against both types of bacteria. Compound (P11) was found to have highest activity against both Gram-positive and Gram-negative bacteria.

scholarly journals GC-MS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF STEREOSPERMUM FIMBRIATUM AGAINST SELECTED SKIN-ASSOCIATED PATHOGENS

2021 ◽  
Vol 83 (5) ◽  
pp. 67-73
Author(s):  
Anis Fadhlina Izyani Awang ◽  
Zaidul Islam Sarker ◽  
Fadzilah Adibah Abdul Majid ◽  
Hassan I. Sheikh
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Stem Bark ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Plant Parts ◽  
Ms Analysis ◽  
Diffusion Assay ◽  
Phytochemical Contents

Stereospermum fimbriatum (local name: “Chicha”) has been used traditionally to treat postpartum illness, stomachache, earache and itchy skin in Malaysia. This study was performed to investigate the phytochemical contents of S. fimbriatum (flowers, leaves, twig and stem bark) by phytochemicals screening and GC-MS analysis as well as to determine their antimicrobial potential against eleven skin-associated pathogens. The extraction was done by soxhlet extractor using n-hexane (Hex), dichloromethane (DCM) and methanol, successively. The antimicrobial activity of all extracts was screened by disc diffusion assay and selected active extracts were evaluated for their minimum inhibitory and bactericidal concentration (MIC & MBC). Phytochemicals such as steroids, terpenoids, tannins, flavonoids and saponins were identified in different plant parts of S. fimbriatum. Hex and DCM extracts of stem bark were the most potent extracts especially against three susceptible pathogens, methicillin-resistant Staphylococcus aureus (13-15 mm), S. aureus (14-15 mm) and S. epidermidis (16 mm). The range of MIC values for Hex and DCM extracts was 0.4 to 0.8 mg/mL. GC-MS analysis of Hex and DCM extracts showed the presence of numerous bioactive compounds. The stem bark of S. fimbriatum was rich of bioactive compounds and exhibited potent antibacterial activity specifically against Staphylococci sp.

scholarly journals Chemical Composition of the Essential Oil from the Leaves of Carapa guianensis Collected from Venezuelan Guayana and the Antimicrobial Activity of the Oil and Crude Extracts

2013 ◽  
Vol 8 (11) ◽  
pp. 1934578X1300801
Author(s):  
Gina Meccia ◽  
Patricia Quintero ◽  
Luis B. Rojas ◽  
Alfredo Usubillaga ◽  
Judith Velasco ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Methanolic Extract ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Minimal Inhibitory Concentrations ◽  
Carapa Guianensis ◽  
Crude Extracts ◽  
Diffusion Assay

The essential oil obtained by hydrodistillation of Carapa guianensis Aubl. (Meliaceae) leaves was analyzed by GC-FID and GC-MS. Twenty-three components were identified, which made up 93.7% of the oil. The most abundant constituents were bicyclogermacrene (28.5%), α-humulene (17.2%), germacrene B (11.9%), and trans-β-caryophyllene (9.9%). Antimicrobial activity of the essential oil, as well as the crude extracts of the leaves obtained by refluxing the dried leaves with n-hexane, dichloromethane, and methanol, was determined using the disc diffusion assay. Activity against Staphylococcus aureus ATCC 29923 and Enterococcus faecalis ATCC 29212 was only found for the essential oil and the methanolic extract, at minimal inhibitory concentrations (MIC) of 400 μg/mL and 50 μg/mL.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Cordia verbenacea from the Venezuelan Andes

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Gina Meccia ◽  
Luis B. Rojas ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Alfredo Usubillaga ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Germacrene D ◽  
Diffusion Assay

The essential oil of Cordia verbenacea D.C. (Boraginaceae) that grows in Mérida-Venezuela was obtained by hydrodistillation from the aerial parts of the plant, yielding 0.21%. The oil was analyzed by GC-FID and GC-MS. Thirty one components which made up 94.3% of the oil were identified. The most abundant constituents found were: tricyclene (23.9%), bicyclogermacrene (11.7%), germacrene D (9.9%) and β-caryophyllene (8.2%). Antibacterial activity determination was carried out according to the disc diffusion assay. Activity against Gram-positive bacteria Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212, at a minimal inhibitory concentration (MIC) of 170 μg/mL and 200 μg/mL, was found.

scholarly journals Antimicrobial activity of endophytic fungi isolated from different plant parts of Curcuma mangga Valeton and Zijp

2021 ◽  
Vol 9 (1) ◽  
pp. 37-42
Author(s):  
Kharul Azmi Muazzam Abdul Rahman ◽  
Darah Ibrahim
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Antimicrobial Activities ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Plant Parts ◽  
Gram Positive Bacteria ◽  
Agar Plug ◽  
Quantitative Screening ◽  
Diffusion Assay

The endophytic fungi isolated from different plant parts including leaf, stem and rhizome of Curcuma mangga were screened for antimicrobial activity by employing agar plug diffusion assay and disc diffusion assay for primary screening and secondary screening, respectively. A total of 127 endophytic fungi that were successfully isolated from various plant parts were cultured to examine their antimicrobial activities. Qualitative screening using agar plug diffusion assay revealed that 118 isolates (92.9%) showed antimicrobial activity against at least on one test microorganisms and suggested that the rhizome part exhibited the highest percentage of antiyeast (58.3%) and antifungal (91.7%) activities compared to leaf and stem parts. Quantitative screening using disc diffusion assay indicated that ethyl acetate extract from fermentative broth (extracellular compound) demonstrated better antimicrobial activity compared to methanol extract derived from fungal biomass (intracellular compound) against all the four classes of pathogenic microorganisms tested (Gram-positive bacteria, Gram-negative bacteria, yeast and fungi). The future of endophytic fungus study is very promising as it possesses hidden potential to be developed as natural antimicrobial agent.

scholarly journals Comparative Analysis of In vitro Antimicrobial and Antioxidant Potential of Cinnamomum tamala Extract and their Essential Oils of Two Different Chemotypes

2021 ◽  
Author(s):  
Gaurav Naik ◽  
Syed Zafar Haider ◽  
Ujjwal Bhandari ◽  
Hema Lohani ◽  
Nirpendra Chauhan
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Diffusion Assay

Background: The essential oils of aromatic plants have wide range of biological applications. Natural food preservatives have been always a demanding for food industries in both developed and developing countries to prevent bacterial growth in food stuffs. Therefore, focused on Cinnamon leaves essential oils components against food pathogens have been investigated to confirm its potential use in food products. Methods: The antimicrobial activity of two Cinnamon leaves oils and extracts (T-2 and T-19) were examined by disc diffusion assay and the minimum inhibitory concentration by two-fold serial dilution method against foodborn pathogenic microorganisms i.e. E.coli (MTCC 723), B. Cereus (MTCC 430), S. aureus (MTCC 3381), S. typhi (MTCC 734) and C. perfringens (MTCC 1349). The antioxidant activity of both essential oils and extract was determined by DPPH assay. The chemical profiling of Cinnamon essential oils were determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Result: The antimicrobial activity of both Cinnamon leaves oils and extract were evaluated by disc diffusion assay and it showed that in essential oils and extracts exhibited the highest zone of inhibition (ZOI) against S. aureus and E.coli. Minimum inhibitory concentration (MIC) of both oils and extracts ranged from 0.156 mg/ml to 5mg/ml and the antioxidant properties of oils and extract of cinnamaldehyde type Cinnamon possessed the highest antioxidant activity than linalool type. The chemical constituent of Cinnamon oil was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) revealed that T-2 contains cinnamaldehyde (75%) and T-19 contains Linalool (63.77%) were found as major constituents. Therefore the results shows that essential oil of cinnamaldehyde type Cinnamon could be a potential rich source of natural antioxidants and also more effective against food borne pathogens than linalool type and could be used as natural antibacterial agents in food preservation.

scholarly journals Antibacterial Screening of Gunnera perpensa-Mediated Silver Nanoparticles

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Naazlene Patel ◽  
Kabange Kasumbwe ◽  
Viresh Mohanlall
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Bacterial Strains ◽  
Antibacterial Screening ◽  
Methods Of Synthesis ◽  
Amine Groups ◽  
Biosynthesized Agnps ◽  
Physical And Chemical ◽  
Diffusion Assay

The biosynthesis of nanoparticles has become quite popular and has been proposed as an alternative over the tedious, expensive, and toxic physical and chemical methods of synthesis due to its cost-effectiveness and ecofriendliness. This study involved the biosynthesis, characterization, and evaluation of the antibacterial activity of Gunnera perpensa-mediated AgNPs. Biosynthesized AgNPs were characterized using TEM, UV, and FTIR spectroscopy. The antimicrobial activity was evaluated in six bacterial strains, using the disc diffusion assay, and MIC was determined using the broth dilution assay. All NPs generally presented as spherical clusters, with sizes ranging from 13 to 24 nm, as determined by TEM. The absorption peaks ranging between 421 and 425 nm and the presence of the C=O bond with amine groups, as indicated by UV and FTIR spectra, confirmed the synthesis and stabilization of G. perpensa extract-mediated AgNPs. Good antimicrobial activity ranging from 7.0 to 9.0 mm was exhibited by both preparations of G. perpensa extract-mediated AgNPs against both Gram-positive and Gram-negative studied bacteria, at MIC ranging from 3.2 to 12.5 μg/ml. Overall, good antibacterial activity was achieved at lower doses with both preparations of the G. perpensa-mediated AgNPs against all tested bacterial strains, suggesting G. perpensa-mediated AgNPs as good antimicrobial agents.

scholarly journals Synthesis and Study of Some Newer Analogues ofQuinolin-8-ol as Potent Antimicrobial Agents

2008 ◽  
Vol 5 (s1) ◽  
pp. S1008-S1014
Author(s):  
Jyoti Sharma ◽  
Sabir Hussain ◽  
Mohammad Amir
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Antifungal Agents ◽  
Gram Negative ◽  
E Coli ◽  
H Nmr ◽  
Gram Negative Organisms ◽  
New Compounds

New series of 4-(substituted phenyl)-5-[(quinolin-8-yloxy) methyl]-4H-1,2,4-triazole-3-thiol(4a-c),N³-(4-substituted phenyl)-5-[(quinolin-8-yloxy) methyl]-4H-1,2,4-triazole-3,4-diamine(5a-c)andN-(4-substituted phenyl)-5-[(quinolin-8-yloxy) methyl]-1,3,4-thiadiazol-2-amine(6a-g)have been synthesized and the structures of the new compounds were established on the basis of IR,1H NMR spectral data.In vitroantimicrobial activity (MIC activity) was evaluated and compared with standard drugs of loxacin and ketoconazole. Compounds5aand6ein the series have shown interesting antibacterial activity against bothS. aureus(gram-positive) andE. coli(gram-negative) organisms. In this paper, we also describe studies leading to identification of antifungal agents. In the gratifying result, most of the compounds were found to have moderate antimicrobial activity.

scholarly journals HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Nida Habib ◽  
Shahnaz Choudhry
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Nigella Sativa ◽  
Qualitative Assessment ◽  
Bacillus Species ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Successive Extraction ◽  
Inhibition Zones ◽  
Diffusion Assay

The medicinal importance of Nigella sativa seeds for treating various ailments is portrayed by its traditional uses. Owing to its immense pharmacological importance, the thymoquinone phytoconstituent of N. sativa can prove beneficial for the South Asian countries including Pakistan, where this seed is commonly produced and healthcare facilities are limited. In this study, the antibacterial activity of various extracts of N. sativa seeds, extracted thymoquinone, and oil samples have been investigated against Bacillus subtilis and Bacillus licheniformis using well and disc diffusion assay. The inhibition zones ranged between 7 and 44 mm against both the bacterial strains by well diffusion assay, while disc diffusion assay provided inhibition zones in the range of 7–23 mm. Commercial and local Kalonji oil samples were included in the study. Oil samples dissolved in methanol showed increased inhibition of bacteria. However, the extracted thymoquinone showed highest antibacterial activity. Medicine formulated using thymoquinone will prove to be an herbal alternate against the resistant microbiota associated with bacterial infections. Antibacterial activity against some Bacillus species will help signify the effect on normal gut flora when oral therapy is followed. Trying different extraction protocols can help increase extraction efficiency. Study on extraction of thymoquinone in local produce of black seed can be fruitful for conducting the stability studies and can help to gain maximum benefits from the bioactives. The crude extracts from 10 g of these seeds were subjected to preliminary phytochemical investigation. Results showed that although methanol extract had the presence of maximum phytochemicals, hexane extract was the most potent in terms of antibacterial activity. Thymoquinone, a therapeutically important bioactive in N. sativa seed, was extracted employing both solvents. TLC assay and UV spectroscopy were used for its qualitative assessment, while HPLC-UV quantification showed that 250 mg/mL of methanol extract had 368.3 μg/mL thymoquinone, while its successive extraction yielded 32.94 μg/mL thymoquinone.

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