scholarly journals Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants

2009 ◽  
Vol 2009 ◽  
pp. 1-11 ◽  
Author(s):  
Turgay Unver ◽  
Deana M. Namuth-Covert ◽  
Hikmet Budak
Keyword(s):  
Gene Expression ◽  
Molecular Biology ◽  
Reverse Genetics ◽  
Methodological Approach ◽  
Forward Genetics ◽  
Methodological Approaches

Advances in molecular biology have led to some surprising discoveries. One of these includes the complexities of RNA and its role in gene expression. One particular class of RNA called microRNA (miRNA) is the focus of this paper. We will first briefly look at some of the characteristics and biogenesis of miRNA in plant systems. The remainder of the paper will go into details of three different approaches used to identify and study miRNA. These include two reverse genetics approaches: computation (bioinformatics) and experimental, and one rare forward genetics approach. We also will summarize how to measure and quantify miRNAs, and how to detect their possible targets in plants. Strengths and weaknesses of each methodological approach are discussed.

THE METHODS OF A RESEARCH OF PROCESSES OF SPONTANEOUS SOCIALIZATION BASED ON NONCLASSICAL APPROACH

2020 ◽  
Vol 22 (12) ◽  
pp. 34-38
Author(s):  
Abashina A.D.
Keyword(s):  
Methodological Approach ◽  
Social Situation ◽  
Subject Domain ◽  
Child Research ◽  
Research Problems ◽  
Internet Environment ◽  
Anthropological Approach ◽  
Research Complex ◽  
Methodological Approaches ◽  
The Individual

Relevance and statement of a problem. Now processes of socialization of younger generation undergo profound changes. They are characterized by transformation of space-time characteristics – narrowing of the field purposeful, expansion of processes of spontaneous socialization. At the same time the methodological approaches and methods of a research aimed at the analysis of the static phenomena applied in pedagogics become insufficient for a research of chaotic processes. There is a need for search of methodology and methods of a research within which the analysis of processes of spontaneous socialization of modern children and teenagers is possible. Research search shows that the solution of this task is possible on the basis of nonclassical methodological approach. Research objective: identification of opportunities of nonclassical methodology for a research of processes of spontaneous socialization of the modern child. Research problems: representation of the methods in logic of nonclassical methodology aimed at the analysis of these processes. Object and subject of research: the situation of development of the child which is characterized by experiences concerning the relations and readiness for an exception of social interaction in various spheres of activity and immersion in the Internet environment. Subject domain of a research: complex of the relations which are the cornerstone of purposeful and spontaneous socialization of the teenager. Research methodology - nonclassical (anthropological) approach. Research materials. In the course of work on a problem the research methods based mainly on the individual and communicative practicians aimed at the analysis of experiences and communication of the child were developed. Results of a research. The qualitative methods based nonclassical approach will allow to understand not only experiences of the child, but also as negative trends under what conditions they lead to break in relations and to search of significant network contacts that is under what conditions processes of purposeful socialization are weakened collect in his social situation of development, extend borders of socialization spontaneous.

METHODOLOGICAL APPROACH TO EVALUATING THE EFFECTIVENESS OF RESOURCE USE BY MEDICAL ORGANIZATIONS

2020 ◽  
Vol 3 (7) ◽  
pp. 62-69
Author(s):  
S. S. BUDARIN ◽  
Keyword(s):  
Medical Care ◽  
Resource Use ◽  
Methodological Approach ◽  
Use Of Resources ◽  
Quality Of Medical Care ◽  
Methodological Approaches ◽  
Performance Audit ◽  
Available Resources

The article reveals methodological approaches to evaluating the effectiveness of the use of resources of medi-cal organizations in order to improve the availability and quality of medical care based on the application of the methodology of performance audit; a methodological approach to the use of individual elements of the efficiency audit methodology for evaluating the performance of medical organizations and the effectiveness of the use of available resources is proposed.

scholarly journals Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nathan J. VanDusen ◽  
Julianna Y. Lee ◽  
Weiliang Gu ◽  
Catalina E. Butler ◽  
Isha Sethi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genetic Screen ◽  
Forward Genetics ◽  
Epigenetic Mark ◽  
Biological Processes ◽  
Dynamic Changes ◽  
Forward Genetic Screen ◽  
High Resource ◽  
Organ Systems

AbstractThe forward genetic screen is a powerful, unbiased method to gain insights into biological processes, yet this approach has infrequently been used in vivo in mammals because of high resource demands. Here, we use in vivo somatic Cas9 mutagenesis to perform an in vivo forward genetic screen in mice to identify regulators of cardiomyocyte (CM) maturation, the coordinated changes in phenotype and gene expression that occur in neonatal CMs. We discover and validate a number of transcriptional regulators of this process. Among these are RNF20 and RNF40, which form a complex that monoubiquitinates H2B on lysine 120. Mechanistic studies indicate that this epigenetic mark controls dynamic changes in gene expression required for CM maturation. These insights into CM maturation will inform efforts in cardiac regenerative medicine. More broadly, our approach will enable unbiased forward genetics across mammalian organ systems.

scholarly journals Recent Progress in Torovirus Molecular Biology

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 435
Author(s):  
Makoto Ujike ◽  
Fumihiro Taguchi
Keyword(s):  
Molecular Biology ◽  
Recent Progress ◽  
Reverse Genetics ◽  
Cultured Cells ◽  
Host Adaptation ◽  
Economic Losses ◽  
New Family ◽  
Enteric Diseases ◽  
Long Time ◽  
Medical Importance

Torovirus (ToV) has recently been classified into the new family Tobaniviridae, although it belonged to the Coronavirus (CoV) family historically. ToVs are associated with enteric diseases in animals and humans. In contrast to CoVs, which are recognised as pathogens of veterinary and medical importance, little attention has been paid to ToVs because their infections are usually asymptomatic or not severe; for a long time, only one equine ToV could be propagated in cultured cells. However, bovine ToVs, which predominantly cause diarrhoea in calves, have been detected worldwide, leading to economic losses. Porcine ToVs have also spread globally; although they have not caused serious economic losses, coinfections with other pathogens can exacerbate their symptoms. In addition, frequent inter- or intra-recombination among ToVs can increase pathogenesis or unpredicted host adaptation. These findings have highlighted the importance of ToVs as pathogens and the need for basic ToV research. Here, we review recent progress in the study of ToV molecular biology including reverse genetics, focusing on the similarities and differences between ToVs and CoVs.

scholarly journals Recent Advances in the Development of Exogenous dsRNA for the Induction of RNA Interference in Cancer Therapy

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 701
Author(s):  
Tatiana S. Golubeva ◽  
Viktoria A. Cherenko ◽  
Konstantin E. Orishchenko
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Molecular Biology ◽  
Gene Silencing ◽  
Regulation Of Gene Expression ◽  
Research Area ◽  
Disease Treatment ◽  
Practical Applications ◽  
Agricultural Plants ◽  
Insect Reproduction

Selective regulation of gene expression by means of RNA interference has revolutionized molecular biology. This approach is not only used in fundamental studies on the roles of particular genes in the functioning of various organisms, but also possesses practical applications. A variety of methods are being developed based on gene silencing using dsRNA—for protecting agricultural plants from various pathogens, controlling insect reproduction, and therapeutic techniques related to the oncological disease treatment. One of the main problems in this research area is the successful delivery of exogenous dsRNA into cells, as this can be greatly affected by the localization or origin of tumor. This overview is dedicated to describing the latest advances in the development of various transport agents for the delivery of dsRNA fragments for gene silencing, with an emphasis on cancer treatment.

Collaborative Self-Ethnography: Navigating Self-Reflexivity in a Sport Management Context

2015 ◽  
Vol 29 (5) ◽  
pp. 498-509 ◽  
Author(s):  
Shannon Kerwin ◽  
Larena Hoeber
Keyword(s):  
Sport Management ◽  
Methodological Approach ◽  
Theory Development ◽  
Personal Reflection ◽  
Management Research ◽  
Sport Fans ◽  
Management Context ◽  
Methodological Approaches

The main goal of our article is to encourage personal reflection within the field of sport management as a tool to strengthen methodological approaches in our research. We explore and discuss the utility of collaborative self-ethnography as one way to acknowledge personal identities through a reflexive account of our experiences as sport fans and sport researchers with this methodology. We draw on a previous study of our experiences as sport fans to illustrate techniques, downfalls, and benefits of studying one’s experiences in a collaborative methodological approach. We have two objectives: First, we hope to encourage sport management researchers to acknowledge and reflect on their personal identities related to sport, such as being a fan, coach, volunteer, or former participant, in their research. Second, we aim to demonstrate the utility of collaborative self-ethnography as one way to incorporate reflexivity in sport management research and theory development.

Methodological approaches to assessing the digitalization scale in types of economic activity in the region and the potential of digital technologies for the real economy

2021 ◽  
Vol 17 (8) ◽  
pp. 1519-1541
Author(s):  
Vitalii V. PECHATKIN ◽  
Liliya M. VIL'DANOVA
Keyword(s):  
Methodological Approach ◽  
Import Substitution ◽  
Digital Technologies ◽  
Industrial Sector ◽  
Digital Transformation ◽  
Real Economy ◽  
Economic Activities ◽  
The Real ◽  
Industrial Enterprises ◽  
Methodological Approaches

Subject. As digital technologies spread across all industries, active processes of digital transformation need to be managed both nationally and regionally. Assessing the extent of digitalization across types of economic activities is the key issue for setting up the socio-economic development strategy of the region and evaluating its efficiency. Objectives. The study is aimed to formulate and test methodological approaches to assessing the digitalization in types of economic activities and the potential of digital technologies for the real economy. Methods. The study relies upon the dialectical method, systems approach, questionnaires, expert approach, interpretation of empirical facts through tables, etc. Results. We devised a methodological approaches to assessing the extent of digitalization in types of economic activities across regions. The approach combines the quantification and evaluation of the process and helps determine the extent of local digital transformation at the regional level. We devised and tested the methodological approach to rating digital technologies, which have the high potential for raising the competitiveness and resilience to competition of the industrial sector in the Russian regions. As opposed to the existing approaches, the approach accounts for the current scale of digital technologies in the national economy, the potential for growth in the demand and supply in the domestic and foreign markets, and the potential for import substitution with respect to foreign technologies and products. Conclusions and Relevance. What makes the proposed methodological approaches more preferable is that they help assess not only the extent of digitalization in types of economic activities and the predominance of certain types in industrial enterprises, but also determine their potential for import substitution in terms of digital security.

METHODOLOGICAL EURO-ORIENTATIONS FOR EVALUATION OF ECOSYSTEM ASSETS OF TERRITORIAL COMMUNITIES RELATED TO WATER RESOURCES

2020 ◽  
pp. 41-47
Author(s):  
Valentyna Kolmakova
Keyword(s):  
Local Level ◽  
Methodological Approach ◽  
Local Communities ◽  
Asset Valuation ◽  
Strategic Approach ◽  
European Experience ◽  
Key Characteristics ◽  
Methodological Approaches ◽  
Methodological Principles ◽  
Spatial Entities

The purpose of the article is to substantiate the theoretical and methodological approach to determining the key characteristics of the assessment of ecosystem assets of territorial communities related to water. The study considers the theoretical and methodological principles of assessing ecosystem assets for sustainable development of local communities. The initial methodological approaches of the accumulated world experience on the assessment of ecosystem services and ecosystem assets of local level territories in the context of three components (ecological, economic and social) in the context of water-related ecosystems are specified. An algorithm for estimating ecosystem assets is proposed. The key guidelines for assessing the ecosystem assets of territorial spatial entities based on European experience are revealed. The list of scientific recommendations for the assessment of water-related ecosystem assets to enhance the capacity of local communities and preserve and restore ecosystems is substantiated. The novelty of the study lies in the proposals for the implementation in Ukrainian practice of general approaches to the methodology of assessment of ecosystem assets and services, according to the recommendations of the international project of the European Commission “The Economics of Ecosystems and Biodiversity” (TEEB). The key Euro-benchmarks proposed by the author for the assessment of water-related ecosystem assets will help increase the investment attractiveness of spatial territorial formations and create preconditions for the development of a new economy on an ecosystem basis. Further research has prospects in the following areas: formation of a comprehensive strategic approach to the introduction of ecosystem asset valuation at the local level; development and introduction of effective methodological approaches to the assessment of ecosystem assets for the formation of investment attractiveness of the territory through the use of local natural resources, including water, as ecosystem assets of sustainable spatial development.

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