scholarly journals Serum Resistin and Insulin-Like Growth Factor-1 Levels in Patients with Hypothyroidism and Hyperthyroidism

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ceren Eke Koyuncu ◽  
Sembol Turkmen Yildirmak ◽  
Mustafa Temizel ◽  
Tevfik Ozpacaci ◽  
Pinar Gunel ◽  
...  
Keyword(s):  
Growth Factor ◽  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Enzyme Linked Immunosorbent Assay ◽  
Insulin Like Growth Factor ◽  
Healthy Individuals ◽  
Control Groups ◽  
Chemiluminescence Method ◽  
And Control ◽  
Hyperthyroid Patients

Introduction. The aim of this study was to evaluate the serum levels of resistin and insulin-like growth factor-1 (IGF-1) and and also the potential relationship between thyroid function and levels of resistin and IGF-1 in hypothyroid and hyperthyroid patients.Methods. Fifteen cases of hypothyroid (HT), 16 of subclinically hypothyroid (SCHT), 15 of hyperthyroid (HrT), 15 of subclinically hyperthyroid (SCHrT), and 17 healthy individuals have been included in the study. Serum resistin levels were measured using enzyme-linked immunosorbent assay and IGF-1 and thyroid stimulating hormone (TSH) levels by chemiluminescence method.Results. Resistin levels in total HT group were significantly higher than in controls (12.66 ± 6.04 and 8.45 ± 2.90 ng/mL, resp.). In SCHrT subgroup resistin levels were significantly higher than those of controls (14.88 ± 7.73 and 8.45 ± 2.90 ng/mL, resp.). IGF-1 levels were significantly lower in total HT than in total HrT and control groups (117.22 ± 52.03, 155.17 ± 51.67, and 184.00 ± 49.73 ng/mL, resp.). Furthermore IGF-1 levels in HT subgroup were significantly lower compared to controls (123.70 ± 44.03 and 184 ± 49.73 ng/mL, resp.). In SCHT subgroup IGF-1 levels were significantly lower than those of control and SCHrT groups (111.11 ± 59.35, 184.00 ± 49.73, and 166.60 ± 47.87 ng/mL, resp.). There were significant correlations between IGF-1 and TSH in HT subgroup and between resistin and TSH in total HrT group.Conclusion. It was concluded that increased resistin levels are directly related to thyroid dysfunction, and GH/IGF-1 axis is influenced in clinically or subclinically hypothyroidism patients.

scholarly journals Changes in interleukin-27 levels in patients with acute coronary syndrome and their clinical significance

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e5652 ◽  
Author(s):  
Lin Zhang ◽  
Junfeng Zhang ◽  
Shaohong Su ◽  
Suyan Luo
Keyword(s):  
Acute Coronary Syndrome ◽  
Angina Pectoris ◽  
Th17 Cells ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Groups ◽  
Coronary Syndrome ◽  
Significant Difference ◽  
Ifn Γ ◽  
And Control

Background This study evaluated changes in interleukin (IL)-27 levels in patients with acute coronary syndrome (ACS) and their influence on Th1, Th2, and Th17 cells. Methods Serum levels of IL-27, IL-4, IL-17, and interferon (IFN)-γ in healthy subjects as well as patients with ACS, including stable angina pectoris (SA), unstable angina pectoris (UA), and acute myocardial infarction (AMI), were determined using an enzyme-linked immunosorbent assay. The proportions of Th1, Th2, and Th17 cells among peripheral blood mononuclear cells (PBMCs), were measured using flow cytometry, after incubation with phorbol myristate acetate (PMA) for 4 h. The proportions of Th1 and Th17 cells among PBMCs in AMI and UA were detected after stimulation with IL-27 or PMA + IL-27 for 4, 8, and 12 h. Results Serum levels of IL-27 in patients with AMI and UA were significantly lower than those in SA and control groups, while serum levels of IL-17 and IFN-γ in AMI and UA groups were dramatically increased compared to those in SA and healthy control groups. However, there were no statistically significant differences in serum IL-4. The proportions of Th1 and Th17 cells among PBMCs were statistically significantly higher in the AMI and UA groups than those in the SA and control groups, while there was no statistically significant difference in the proportion of Th2 cells among different groups. For patients with AMI and UA, the effect of co-stimulation of PBMCs with PMA and IL-27 was not significantly different from that of PMA single stimulation, while PMA + IL-27 co-stimulation lowered the Th17 cell proportion significantly compared to PMA single stimulation. Discussion Compared to SA patients and healthy controls, patients with ACS (AMI + UA) had lower serum levels of IL-27 and higher proportions of PBMC Th1 and Th17 cells, which could be attributed to the inhibitory effects of IL-27 on the proliferation of Th17 cells. These results indicated that IL-27 could be a novel therapeutic target in ACS patients.

scholarly journals IGFBP-4: A promising biomarker for lung cancer

2021 ◽  
Vol 40 (3) ◽  
pp. 237-244
Author(s):  
Savas Nur ◽  
Akin Ozturk ◽  
Murat Kavas ◽  
Ismet Bulut ◽  
Sumeyye Alparslan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Growth Factor ◽  
Protein A ◽  
Serum Levels ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Healthy Individuals ◽  
Treatment Status ◽  
Patient Groups ◽  
Lung Cancer Biomarker

Background: Insulin-like growth factor binding protein-4 (IGFBP-4), a member of the insulin-like growth factor (IGF) family, transports, and regulates the activity of IGFs. The pregnancy-associated plasma protein-A (PAPP-A) has proteolytic activity towards IGFBP-4, and both proteins have been associated with a variety of cancers, including lung cancer. Thus, we aimed to evaluate the use of IGFBP-4 and PAPP-A as potential biomarkers for lung cancer. Methods: Eighty-three volunteers, including 60 patients with lung cancer and 23 healthy individuals, were included in this study. The patients with lung cancer were selected based on their treatment status, histological subgroup, and stage of the disease. Enzyme-linked immunosorbent assays were used to assess the serum levels of IGFBP-4 and PAPPA, whereas the IGF-1 levels were measured using a chemiluminescent immunometric assay. Results: The serum IGFBP-4 levels in all patient groups, regardless of the treatment status and histological differences, were significantly higher than those in the control group (p < 0.005). However, the serum PAPP-A levels in the untreated patient group were found to be higher than those in the control group, but this difference was not statistically significant (p = 0.086). Conclusions: The serum PAPP-A and IGFBP-4 levels are elevated in lung cancer. However, IGFBP-4 may have better potential than PAPP-A as a lung cancer biomarker.

scholarly journals Effect of satiety on TSH and free thyroxin levels in healthy individuals and patients with subclinical and overt hypothyroidism

2021 ◽  
Vol 16 (4) ◽  
pp. 28-32
Author(s):  
Mehmet G. Kılınçarslan ◽  
Cihan Çelik ◽  
Erkan M. Şahin
Keyword(s):  
Tertiary Hospital ◽  
Thyroid Stimulating Hormone ◽  
Free Thyroxine ◽  
Overt Hypothyroidism ◽  
Fasting State ◽  
Healthy Individuals ◽  
Control Groups ◽  
And Control ◽  
Tsh Levels ◽  
Paired T Test

Background: Thyroid stimulating hormone (TSH) and thyroxine levels may change according to fasting — satiety status. AIM: The aim of this study was to determine the effect of satiety on TSH and free thyroxine levels.Methods: This study was conducted in a tertiary hospital. According to previous TSH and thyroxine levels, groups of 30, 30 and 60 participants were designated as subclinical hypothyroidism, hypothyroidism, and control groups respectively. To obtain TSH and thyroxine results first phlebotomy was performed at 8 am while participants were in fasting state. Then participants were allowed to have non-standardized breakfast. Second phlebotomy was performed at 10 am while participants were in non-fasting state. Paired t-test and ANOVA were used to analyze the data.Results: The fasting TSH levels of the participants (2.57 ± 1.84 mlU/L) were significantly higher than the satiety TSH levels (2.04 ± 1.48 mlU/L) (t = 8.566, p < 0.001, d = 0.80). The fasting free thyroxine values (1.31 ± 0.38 mg/dl) of the participants were significantly lower than the satiety free thyroxine values (1.39 ± 0.35 mg/dl) (t = -1.988, p = 0.049, d = 0.20).Conclusion: Knowing how TSH and free thyroxine tests are affected by satiety has the power to affect treatment of many patients. Our study has shown that both TSH and free thyroxine levels changed significantly according to satiety status.

scholarly journals The phosphorylation status of insulin-like growth factor-binding protein-1 in prepubertal obese children

1999 ◽  
pp. 585-589 ◽  
Author(s):  
T Kamoda ◽  
H Saitoh ◽  
S Nakahara ◽  
M Inudoh ◽  
T Hirano ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Serum Levels ◽  
Insulin Like Growth Factor ◽  
Obese Children ◽  
Factor Binding ◽  
Igf I ◽  
The Status ◽  
And Control ◽  
Igfbp 3

OBJECTIVE: We measured the total and nonphosphorylated insulin-like growth factor-binding protein (IGFBP)-1 concentrations in obese children to determine the effect of obesity on the status of IGFBP-1 phosphorylation. We also measured the serum levels of insulin, total and free IGF-I, and IGFBP-3 to investigate their relationships to the IGFBP-1 phosphorylation status in obese subjects. SUBJECTS AND METHODS: Nineteen prepubertal obese and 15 age-matched control children were included in the study. The serum levels of total and nonphosphorylated IGFBP-1 were determined by noncompetitive RIAs. RESULTS: The serum levels of total and nonphosphorylated IGFBP-1 were significantly lower in the obese group (48.7+/-5.6 microgram/l, P<0.001 and 11.1+/-1.9 microgram/l, P<0.01 respectively) than in the controls (86.7+/-9.0 microgram/l and 28.8+/-6.2 microgram/l respectively). However, the ratio of nonphosphorylated IGFBP-1 to total IGFBP-1 did not differ significantly between the obese and control groups. The circulating free IGF-I level was significantly higher in the obese children than in the controls (P<0.05), while the serum levels of insulin, total IGF-I and IGFBP-3 were not significantly different between the two groups. A stepwise regression analysis of the combined group revealed that only the total IGFBP-1 level was an independent predictor of the free IGF-I concentration (P<0.001). CONCLUSION: The present study shows that both total and nonphosphorylated IGFBP-1 concentrations are decreased in obese children and the increased free IGF-I level in obese children is related to the reduced total IGFBP-1 level, but unrelated to the change in the IGFBP-1 phosphorylation status.

Study the Level of Certain Biochemical Parameters in Iraqi Patients with Thyroid Disorder

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Shatha H Jwaid ◽  
Ahmed G Sabbar ◽  
Amani M Touma
Keyword(s):  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Chloride Ions ◽  
Serum Phosphorus ◽  
Thyroid Disorder ◽  
Control Groups ◽  
Significant Difference ◽  
Method Analysis ◽  
Hyperthyroid Patients ◽  
Phosphorus Levels

Aim: This study aimed to evaluate changes in serum levels of some elements(phosphorus and chloride ions) and relationship with hypothyroidism, hyperthyroidism; and euthyroidism as a control groups. Method: Analysis of serum phosphorus and chloride were done by enzymatic methods using AGAPPE kit. Result: Patients with hyperthyroidism and hypothyroidism showed highly significant increase in serum phosphorus levels (p less than 0.001). In case of hypo- and hyperthyroidism significant difference could not be obtained among euthyroidism and patients (p>0.05) in chloride level. While a positive correlation with thyroid stimulating hormone (TSH), serum phosphorous and chloride in case of hypothyroidism. In hyperthyroid patients, the correlation was negative for phosphorous and positive for chloride, also none of correlations were statistically significant (p>0.05). Conclusion: Thyroid patients should be regularly checked for serum electrolytes. Early discovery and management can prevent the further complications and will be useful during the controlling of thyroid patients.

scholarly journals Kisspeptin serum levels in acute myocardial infarction patients and healthy individuals

2018 ◽  
Vol 5 (3) ◽  
pp. 2111-2118 ◽  
Author(s):  
Mohammad Shojaei ◽  
Saeideh Erfanian ◽  
Abdolreza Sotoodeh Jahromi ◽  
Naeimeh Yusefi ◽  
Abdolhossein Madani ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Serum Levels ◽  
Serum Concentrations ◽  
Healthy Individuals ◽  
Control Groups ◽  
Blood Samples ◽  
St Segment Elevation ◽  
St Segment ◽  
And Control

Introduction: Acute myocardial infarction (AMI) is a leading cause of mortality worldwide for which several risk factors have been identified. Kisspeptin is a multifunctional peptide observed in atherosclerotic plaques. This study aimed to compare kisspeptin serum levels in AMI patients and healthy individuals. Methods: This case-control study was conducted on patients visiting the Cardiology department of Peymanieh Hospital, Jahrom University of Medical Sciences, Iran. Participants were randomly divided into a case and a control groups; the former consisting of 90 AMI patients and the latter of 90 healthy individuals with no cardiac diseases. Blood samples were collected from the participants who also completed the given questionnaire. The enzyme-linked immunosorbent assay (ELISA) test was used to determine kisspeptin levels following serum separation from collected blood samples. Data analysis was performed in SPSS-19 using descriptive (mean and standard deviation) and analytical (The Mann-Whitney U test) statistical tests. Results: The mean age of treatment and control groups was 60.91 and 61.72 years, respectively. The majority (73.3%) of participants in this study were men. Kisspeptin serum concentrations were demonstrated to be significantly lower in the case group than the control group (P<0.001). The results of Mann-Whitney U test revealed that kisspeptin serum concentrations of case and control groups did not have a significant relationship with such variables as gender, smoking, and ST-segment elevation and non-ST-segment elevation (P>0.05). Conclusion: The results revealed that kisspeptin serum concentrations of AMI patients were significantly lower than those of healthy individuals. Accordingly, kisspeptin deficiency appears to be a risk factor for AMI. However, due to the multifunctional nature of this peptide, further studies are necessary to discover its precise mechanism of action.

scholarly journals Effect of sex on the levels of galanin and adiponutrin in hypertensive patients with obesity

2021 ◽  
Vol 25 (2) ◽  
pp. 281-286
Author(s):  
U. S. Herasymchuk
Keyword(s):  
Complex Disease ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Obese Women ◽  
Control Groups ◽  
Men And Women ◽  
Organ Systems ◽  
Sexual Characteristics ◽  
And Control

Annotation. Hypertension is a complex disease that affects several organ systems and today remains the leading modified risk factor for cardiovascular disease, which is a major cause of disability and mortality in both men and women. The aim of the study was to assess the peculiarities of hormonal activity of adipose tissue, namely serum levels of galanin and adiponutrin, in hypertension in combination with obesity in men and women. 58 people were examined, including 32 women and 26 men with 1-3 degrees of hypertension and 20 healthy people. Patients were measured blood pressure in a sitting position after 5 minutes of rest, performed anthropometric measurements, evaluated lipid profile, carbohydrate metabolism. To determine the level of adiponutrin and galanin, an enzyme-linked immunosorbent assay was used. Statistical computer processing of the results was performed using the software package “Statistica 10” (StatSoft Inc.). Significantly higher serum levels of galanin were found in the main group of women compared with the control group (147.99 vs. 30.58 pkg / ml, p<0.001), and higher values were in the group with hypertension of degree 3 as opposed to 1-2 degrees (166.48 pkg / ml vs. 52.64 pkg / ml, p<0.001). Patients with this comorbid pathology had higher serum levels of adiponutrin in men (6.94 vs. 1.55 pkg / ml, respectively, the main and control groups, p<0,001), but given the degree of obesity, the prevalence was in obese women 2-3 degrees (14.15 vs. 11.33 pkg / ml). Thus, based on the above, we can assume that the degree of hypertension and obesity are provoking factors in increasing these adipokines. In the future, it is advisable to conduct further studies to assess the activity of adipokines, namely galanin and adiponutrin in view of sexual characteristics.

Study the Level of Certain Biochemical Parameters in Iraqi Patients with Thyroid Disorder

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Shatha H. Jwaid ◽  
Ahmed G. Sabbar ◽  
Amani M Touma
Keyword(s):  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Chloride Ions ◽  
Serum Phosphorus ◽  
Thyroid Disorder ◽  
Control Groups ◽  
Significant Difference ◽  
Method Analysis ◽  
Hyperthyroid Patients ◽  
Phosphorus Levels

Aim: This study aimed to evaluate changes in serum levels of some elements (phosphorus and chloride ions) and relationship with hypothyroidism, hyperthyroidism; and euthyroidism as a control groups. Method: Analysis of serum phosphorus and chloride were done by enzymatic methods using AGAPPE kit. Result: Patients with hyperthyroidism and hypothyroidism showed highly significant increase in serum phosphorus levels (p less than 0.001). In case of hypo- and hyperthyroidism significant difference could not be obtained among euthyroidism and patients (p GREATOR THAN 0.05) in chloride level. While a positive correlation with thyroid stimulating hormone (TSH), serum phosphorous and chloride in case of hypothyroidism. In hyperthyroid patients, the correlation was negative for phosphorous and positive for chloride, also none of correlations were statistically significant (p GREATOR THAN 0.05). Conclusion: Thyroid patients should be regularly checked for serum electrolytes. Early discovery and management can prevent the further complications and will be useful during the controlling of thyroid patients.

Low Serum Levels of Fibroblast Growth Factor 2 in Gunn Rats: A Hyperbilirubinemia Animal Model of Schizophrenic Symptoms

2020 ◽  
Vol 19 (7) ◽  
pp. 503-508
Author(s):  
Maiko Hayashida ◽  
Sadayuki Hashioka ◽  
Kenji Hayashida ◽  
Shoko Miura ◽  
Keiko Tsuchie ◽  
...  
Keyword(s):  
Animal Model ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Wistar Rats ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Normal Strain ◽  
Fibroblast Growth ◽  
Gunn Rats

Background: Fibroblast growth factor (FGF) 2 (also referred to as basic FGF) is a multifunctional growth factor that plays a pivotal role in the pro-survival, pro-migration and pro-differentiation of neurons. Method: Because alterations in FGF2 levels are suggested to contribute to the pathogenesis schizophrenia, we investigated serum levels of FGF2 in the Gunn rat, a hyperbilirubinemia animal model of schizophrenic symptoms. Results: The enzyme-linked immunosorbent assay showed that the serum levels of FGF2 in Gunn rats were 5.09 ± 0.236 pg/mL, while those in the normal strain Wistar rats were 11.90 ± 2.142 pg/mL. The serum FGF2 levels in Gunn rats were significantly lower than those in Wistar rats. We also measured serum levels of unconjugated bilirubin (UCB) and found a significant negative correlation between UCB and FGF2 at serum levels in all the rats studied. Conclusion: Since it is known that FGF2 regulates dopaminergic neurons and have anti-neuroinflammatory effects, our finding suggests that low FGF2 levels may contribute to the pathogenesis of schizophrenia, in which disbalanced dopamin-ergic signaling and neuroinflammation are supposed to play certain roles.

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