scholarly journals Changes in interleukin-27 levels in patients with acute coronary syndrome and their clinical significance

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e5652 ◽  
Author(s):  
Lin Zhang ◽  
Junfeng Zhang ◽  
Shaohong Su ◽  
Suyan Luo
Keyword(s):  
Acute Coronary Syndrome ◽  
Angina Pectoris ◽  
Th17 Cells ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Groups ◽  
Coronary Syndrome ◽  
Significant Difference ◽  
Ifn Γ ◽  
And Control

Background This study evaluated changes in interleukin (IL)-27 levels in patients with acute coronary syndrome (ACS) and their influence on Th1, Th2, and Th17 cells. Methods Serum levels of IL-27, IL-4, IL-17, and interferon (IFN)-γ in healthy subjects as well as patients with ACS, including stable angina pectoris (SA), unstable angina pectoris (UA), and acute myocardial infarction (AMI), were determined using an enzyme-linked immunosorbent assay. The proportions of Th1, Th2, and Th17 cells among peripheral blood mononuclear cells (PBMCs), were measured using flow cytometry, after incubation with phorbol myristate acetate (PMA) for 4 h. The proportions of Th1 and Th17 cells among PBMCs in AMI and UA were detected after stimulation with IL-27 or PMA + IL-27 for 4, 8, and 12 h. Results Serum levels of IL-27 in patients with AMI and UA were significantly lower than those in SA and control groups, while serum levels of IL-17 and IFN-γ in AMI and UA groups were dramatically increased compared to those in SA and healthy control groups. However, there were no statistically significant differences in serum IL-4. The proportions of Th1 and Th17 cells among PBMCs were statistically significantly higher in the AMI and UA groups than those in the SA and control groups, while there was no statistically significant difference in the proportion of Th2 cells among different groups. For patients with AMI and UA, the effect of co-stimulation of PBMCs with PMA and IL-27 was not significantly different from that of PMA single stimulation, while PMA + IL-27 co-stimulation lowered the Th17 cell proportion significantly compared to PMA single stimulation. Discussion Compared to SA patients and healthy controls, patients with ACS (AMI + UA) had lower serum levels of IL-27 and higher proportions of PBMC Th1 and Th17 cells, which could be attributed to the inhibitory effects of IL-27 on the proliferation of Th17 cells. These results indicated that IL-27 could be a novel therapeutic target in ACS patients.

Plasma kisspeptin and ghrelin levels in puberty variant cases

2017 ◽  
Vol 30 (5) ◽  
Author(s):  
Erdal Kurnaz ◽  
Yaşar Şen ◽  
Süleyman Aydın
Keyword(s):  
Serum Levels ◽  
Control Group ◽  
Control Groups ◽  
Premature Thelarche ◽  
Premature Adrenarche ◽  
Significant Difference ◽  
And Gender ◽  
And Control ◽  
Relationship Of ◽  
The Relationship

AbstractBackground:The aim of this study was to determine the serum levels of kisspeptin and ghrelin (GAH), as well as the relationship of these two peptides with each other in premature thelarche (PT) and premature adrenarche (PA) cases and to investigate the possibility of using these peptides as markers in the differentiation of puberty disorders.Methods:A PT group aged 1–8 years (n = 40), a PA group aged 1–9 years (n = 23, female/male = 20/3) and control groups consistent with each of the previous groups in terms of age and gender were created for the study. Kisspeptin and ghrelin levels were measured with ELISA methods from blood samples drawn while fasting in the morning.Results:When the PT group was compared with the controls, the plasma kisspeptin levels of the cases were significantly higher than the control group (165.47 ± 15.45 pmol/L, 96.82 ± 12.33 pmol/L, p = 0.005, respectively). Kisspeptin levels in the PA group did not show a difference with the control group (121.36 ± 17.99 pmol/L, 95.52 ± 11.54 pmol/L, p = 0.249, respectively). No significant difference could be found when GAH levels in the PT and PA groups were compared with controls. No significant correlation was found between kisspeptin and GAH levels in the PT and PA groups.Conclusions:Our results indicate that kisspeptin plays an important role in the PT, but GAH is not associated with puberty disorders.

scholarly journals Circulating Th22 and Th9 Levels in Patients with Acute Coronary Syndrome

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Ying-zhong Lin ◽  
Bang-wei Wu ◽  
Zheng-de Lu ◽  
Ying Huang ◽  
Ying Shi ◽  
...  
Keyword(s):  
Angina Pectoris ◽  
Flow Cytometric Analysis ◽  
Control Groups ◽  
Th22 Cells ◽  
Coronary Syndrome ◽  
Flow Cytometric ◽  
Th9 Cells ◽  
Coronary Syndromes ◽  
And Control ◽  
Progression Of Atherosclerosis

Background. CD4+ T helper (Th) cells play critical roles in the development and progression of atherosclerosis and the onset of acute coronary syndromes (ACS, including acute myocardial infarction (AMI) and unstable angina pectoris (UAP)). In addition to Th1, Th2, and Th17 cells, Th22 and Th9 subsets have been identified in humans. In the present study, we investigated whether Th22 cells and Th9 cells are involved in the onset of ACS.Methods. The frequencies of Th22 and Th9 cells were detected using a flow cytometric analysis and their related cytokine and transcription factor were measured in the AMI, UAP, stable angina pectoris (SAP), and control groups.Results. The results revealed a significant increase in the peripheral Th22 number, AHR expression, and IL-22 levels in patients with ACS compared with those in the SAP and control groups. Although there was no difference in the peripheral Th9 number among the four groups, the PU.1 expression and IL-9 levels were significantly increased in patients with ACS compared with the SAP and control groups.Conclusions. Circulating Th22 and Th9 type responses may play a potential role in the onset of ACS symptom.

scholarly journals Alteration of Serum Levels of Cytokines in Schizophrenic Patients before and after Treatment with Risperidone

2019 ◽  
Author(s):  
Esfandiar Azizi ◽  
Ahmad Zavaran Hosseini ◽  
Sara Soudi ◽  
Ahmad Ali Noorbala
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Syndrome Scale ◽  
Tnf Α ◽  
Significant Difference ◽  
Before And After ◽  
Schizophrenic Patients ◽  
Ifn Γ

A growing body of evidence suggests the existence of abnormalities in the immune system of schizophrenic patients. The current study examined serum levels of interleukin (IL) -1β, IL-6, IL-2,interferon(IFN) -γ, and tumor necrosis factor(TNF)-α in schizophrenic patients before and after treatment with risperidone and correlated levels of these cytokines with symptomatology. The study group consisted of 24 schizophrenic patients as defined by Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV) criteria and 24 healthy controls. Serum cytokine levels were examined using enzyme-linked immunosorbent assay (ELISA). Schizophrenic symptomatology was assessed with the Positive and Negative Syndrome Scale (PANSS) questionnaire. The serum levels of TNF-α, IL-1β and IL-6 were significantly higher in participants before treatment compared with the healthy controls and after treatment (p<0.001). IFN-γ and IL-2 levels were significantly lower in participants after treatment compared with before treatment and the healthy controls (p<0.001). Except for IL-6 (p<0.05), there was no significant difference in the levels of TNF-α and IL-1β between the patients receiving treatment and the healthy subjects. Moreover, there was no significant difference in levels of IFN-γ and IL-2 between patients before treatment and the healthy subjects. There were no significant correlations between the concentration of cytokines studied and the PANSS. Positive intercorrelations between the production of IFN-γ and IL-2 were detected for sums of all groups(r=0.33, p=0.005). Clinical improvement of treated patients was associated with a reduction in the studied cytokines. It seems that changes in the cytokines level may play a significant role in the psychopathology of these patients.

Effectiveness of Cardiac Walking on Blood Pressure Among Patients with Acute Coronary Syndrome

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
T. Vidhya ◽  
C. Kanniammal ◽  
Jaideep Mahendra ◽  
G. Valli
Keyword(s):  
Blood Pressure ◽  
Acute Coronary Syndrome ◽  
World Health ◽  
Control Group ◽  
Study Group ◽  
Coronary Syndrome ◽  
Significant Difference ◽  
The World ◽  
Post Test ◽  
And Control

Background: Cardiovascular diseases (CVD) are the leading cause of the mortality in urban and rural India and foremost causes of the death worldwide. Hypertension is a major risk condition that plays a pivotal role regarding the complications for those diagnosed with Acute Coronary Syndrome. According to the World Health Organization (2012), hypertension is the number one cause of mortality in the world and it is a major risk factor for cardiac diseases and stroke. Objectives: i) To identify the level of blood pressure (BP) in pre and post test among study and control group of patients with acute coronary syndrome. ii) Determine the effect of cardiac walking on blood pressure among patients with ACS. iii) Associate the selected back ground variables with the level of systolic BP in the post test among study and control group. Methods: A quasi experimental design was adopted for this study. There are 50 Patients diagnosed with ASC admitted for the treatment and attending cardiac OPD were selected by purposive sampling technique of non- probability type. The intervention cardiac walking given for a period of one month to the study group. Findings: The comparison of before and after systolic and diastolic blood pressure (BP) within study group depicted statistically significant difference at **p less than 0.001 but not in control group. The comparison of post systolic and diastolic BP between study and control group inferred that there was statistically significant difference at **p less than 0.001. Conclusion: Cardiac walking is most effective way to stabilize the blood pressure among patients with ACS in order to prevent further complications such as stroke.

Regulation of Th1/Th2 and Th17/Treg by pDC/mDC imbalance in primary immune thrombocytopenia

2021 ◽  
pp. 153537022110097
Author(s):  
Qinzhi Li ◽  
Yang Liu ◽  
Xiujuan Wang ◽  
Mingling Sun ◽  
Lei Wang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Platelet Count ◽  
Th17 Cells ◽  
Immune Thrombocytopenia ◽  
Serum Levels ◽  
Mrna Levels ◽  
Significant Difference ◽  
Primary Immune Thrombocytopenia ◽  
Healthy Control ◽  
Ifn Γ

This study investigates the regulatory effect of plasmacytoid dendritic cells (pDC)/myeloid dendritic cells (mDC) imbalance on balance of Th1/Th2 and Th17/Treg in primary immune thrombocytopenia (ITP). A total of 30 untreated ITP patients and 20 healthy controls were recruited. Compared with healthy control, the pDC proportion of ITP patients was significantly reduced ( P = 0.004), while the mDC proportion was not significantly changed ( P = 0.681), resulting in a decrease in the pDC/mDC ratio ( P = 0.001). Additionally, compared with controls, serum levels of interleukin (IL)-6, IL-12, and IL-23 were increased in ITP patients ( P < 0.001), and mRNA levels of IL-12p40, IL-12p35, and IL-23p19 were also increased ( P =0.014, P = 0.043, P < 0.001). Compared with the healthy control, the proportion of Th1 and Th17 cells in ITP patients increased ( P = 0.001, P = 0.031). Serum levels of interferon gamma (IFN-γ) and IL-17 in ITP patients also increased ( P = 0.025, P = 0.005). Furthermore, T-bet and RORγt mRNA levels were increased in peripheral blood of ITP patients ( P = 0.018, P < 0.001). Correspondingly, the proportion of Th2 and Treg cells decreased ( P = 0.007, P < 0.001), along with a decrease in serum IL-4 and transforming growth factor beta (TGF-β) ( P = 0.028, P = 0.042), and an increase in GATA-3 mRNA ( P < 0.001). However, there was no significant difference in Foxp3 mRNA levels ( P = 0.587). Pearson correlation analysis showed that the proportion of total dendritic cells (DCs) was positively correlated with IL-12 ( r = 0.526, P = 0.003) and IL-23 ( r = 0.501, P = 0.005) in ITP patients. Th1/Th2 ratio, IFN-γ, and IL-12 levels were negatively correlated with platelet counts ( r = −0.494, P = 0.009; r = –0.415, P = 0.028; r = –0.492, P = 0.032). However, IL-23 was positively correlated with IL-17 ( r = 0.489, P = 0.006) and negatively correlated with platelet count ( r = –0.564, P = 0.001). The ratio of IL-6 and Th17 cells was negatively correlated with platelet count ( r = –0.443, P = 0.014; r = –0.471, P = 0.011). The imbalance of pDC/mDC and the increase of IL-6, IL-12, and IL-23 lead to the increased differentiation of CD4+ T cells into Th1 and Th17 cells, which might be the important mechanisms underlying the imbalance of Th1/Th2 and Th17/Treg in ITP patients.

scholarly journals Levels of interleukin-33 and interleukin-6 in patients with acute coronary syndrome or stable angina

2013 ◽  
Vol 36 (4) ◽  
pp. 234 ◽  
Author(s):  
Cong-Lin Liu ◽  
De-Liang Shen ◽  
Kui Zhu ◽  
Jun-Nan Tang ◽  
Qi-Min Hai ◽  
...  
Keyword(s):  
Acute Coronary Syndrome ◽  
Serum Level ◽  
Interleukin 6 ◽  
Stable Angina ◽  
Serum Levels ◽  
Control Group ◽  
Stable Angina Pectoris ◽  
Control Groups ◽  
Interleukin 33 ◽  
Coronary Syndrome

Purpose: The purpose of this study was to investigate the levels of interleukin-33 (IL-33) and interleukin-6 (IL-6) in patients with acute coronary syndrome or stable angina. Methods: Serum IL-33 and IL-6 were measured with Enzyme Linked Immuosorbent Assay (ELISA) in patients with acute coronary syndrome (ACS, n = 40), and stable angina pectoris (SAP, n = 43). IL-33 and IL-6 were also determined in 30 healthy subjects (control group). Results: The serum level of IL-33 in the ACS group (78.60 ± 44.84 ng/L) was lower than in the SAP (102.58 ± 37.21 ng/L, P < 0.01) or control groups (130.24 ± 10.17 ng/L, P < 0.01). The serum level of IL-6 in the ACS group (39.90 ± 12.64 ng/L) was higher than in the SAP (18.68 ± 11.89 ng/L, P < 0.05) or control groups (6.28 ± 17.72 ng/L, P < 0.05). There were no differences in serum levels of IL-33 and IL-6 among the single-, double- and triple-vessel lesion groups. IL-33 and IL-6 levels were negatively correlated with each other in the ACS (r = -0.871, P < 0.01) and SAP groups (r = -0.788, P < 0.01). Conclusion: The serum level of IL-33 was lower in patients with ACS or SAP and was negatively correlated with the serum level of IL-6. Thus, IL-33 and IL-6 may be used as biomarkers for evaluating inflammatory response and severity of coronary heart disease in patients with ACS or SAP.

scholarly journals High Proteoglycan Decorin Levels Are Associated With Acute Coronary Syndrome and Provoke an Imbalanced Inflammatory Response

2021 ◽  
Vol 12 ◽  
Author(s):  
Lingfang Zhuang ◽  
Yulong Ge ◽  
Xiao Zong ◽  
Qian Yang ◽  
Ruiyan Zhang ◽  
...  
Keyword(s):  
Acute Coronary Syndrome ◽  
Inflammatory Response ◽  
White Blood Cells ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reactive Protein ◽  
Coronary Syndrome ◽  
Control Subjects ◽  
Novel Biomarkers ◽  
And Control

Background and Aims: Acute coronary syndrome (ACS) has become one of the most common causes of disability. It is thus important to identify ACS early in the disease course of patients using novel biomarkers for prompt management. Decorin (DCN) was well-acknowledged for its effect on collagen fibrillogenesis and maintaining tissue integrity. Additionally, DCN could release as secreted proteoglycan under pathological conditions. Hence, we aimed to determine the relationship between serum DCN concentration and ACS.Methods: A total of 388 patients who underwent coronary angiography (CAG) in the cardiovascular center of Ruijin Hospital between June 2016 and December 2017 were enrolled in this study. Blood samples were drawn during CAG surgery to determine the serum DCN level of patients with ACS (n = 210) and control subjects (n = 178) using enzyme-linked immunosorbent assay.Results: We found that the serum DCN levels of ACS patients were elevated compared with those of the control subjects (13.59 ± 0.50 vs. 13.17 ± 0.38, respectively, p &lt; 0.001). Furthermore, the serum DCN level, after being adjusted with other cardiovascular factors, was independently associated with ACS. Moreover, an increased serum DCN level was positively correlated with the number of white blood cells and the level of high-sensitivity C-reactive protein (R = 0.3 and 0.11, respectively). Mechanistically, DCN might have elicited an imbalanced inflammatory response during cardiac ischemia by suppressing the expression of anti-inflammatory genes.Conclusion: Serum DCN is a novel biomarker of ACS and contributes to the increased inflammatory response in ischemic heart disease.

scholarly journals Studying the Frequency of Autoimmune Thyroid Diseases in Duhok Province

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Rozheen I. Hasan ◽  
Amir H. Raziq
Keyword(s):  
Venous Blood ◽  
Serum Levels ◽  
Control Group ◽  
Thyroid Disorder ◽  
Control Groups ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid ◽  
Significant Difference ◽  
The One ◽  
And Control

Thyroid disorders are one of the most frequent disorders affecting endocrine system. The present study aimed to identify the frequency of thyroid disorder in our locality at Duhok city. To do so, a total of one hundred patients and one hundred apparently healthy control individuals were enrolled in this study. Samples of venous blood (5 ml) were withdrawn from patients and control subjects and analysed. Clinical assessment was performed by a specialist and each patient provided with a document supporting preliminary clinical diagnosis. Measurements of serum TSH, T3, T4, and anti-thyroperoxidase antibodies (anti-TPO antibodies) serum levels were performed. All assays were conducted at Duhok central health laboratory. Out of the one hundred patients and the one hundred control groups, forty-two and thirty candidates were randomly selected, respectively. The mean TSH serum levels of 25.87 (uIU/ml) for the patients significantly differ from that of the control group 3.55 (uIU/ml) (p<0.001). However, T3 and T4 serum levels indicated no significant difference between the two categories. Moreover, there was statisticaly significant difference (p< 0.0001) between the patients and control groups when their mean serum anti-TPO concentrations were compared.  In conclusion, considerable number of subjects appeared to be candidates for deranged thyroid functions and is susceptible to autoimmune thyroid disorder.

scholarly journals PLATELET DISTRIBUTION WIDTH VALUE AS A DIAGNOSTIC AND PROGNOSTIC MARKER IN ACUTE CORONARY SYNDROME

2018 ◽  
Vol 24 (2) ◽  
pp. 112
Author(s):  
Rahmi Rifany Latif ◽  
Liong Boy Kurniawan ◽  
Darmawati Rauf
Keyword(s):  
Acute Coronary Syndrome ◽  
Length Of Stay ◽  
Cross Sectional Study ◽  
Large Sample Size ◽  
Platelet Distribution Width ◽  
Cross Sectional ◽  
Distribution Width ◽  
Coronary Syndrome ◽  
Significant Difference ◽  
And Control

An acute coronary syndrome is a form of clinical manifestation of coronary heart disease with atherosclerosis, and thrombosis processes. Platelets play an essential role in thrombosis. High PDW shows a variation of the morphology, and size of a platelet. This was a retrospective cross-sectional study comparing PDW, length of stay, and patient outcome in STEMI, NSTEMI, UAP, and control patients at the Dr.Wahidin Sudirohusodo Hospital Makassar during the period of January 2014 – December 2015. Data were analyzed using statistical software. One hundred, and seventy-two ACS patients were included; 65 patients had UAP, 48 patients had STEMI and 59 patients had NSTEMI. Sixty normal patients were included as a control. Kruskal-Wallis test showed a statistical difference in PDW between patients with STEMI, NSTEMI, UAP, and control (p=0.000). Post hoc test showed a significant difference between ACS, and control, STEMI, and control (p=0.000), NSTEMI and control (p=0.000), UAP, and control (p=0.000), but there no significant difference     between STEMI, and NSTEMI (p=0.320), STEMI, and UAP (p=0.980), NSTEMI and UAP (p=0.435). There was no significant difference in PDW between patients who survived, and died (p=0.298), and also patients with a length of stay of ≤ 7 days, and > 7 days (p=0.293). Platelet distribution width was higher in ACS patients compared with control patients, but could not be used to predict the outcome, and length of stay of patients with ACS. It is advisable to do further research with a large sample size to avoid bias.

Sign in / Sign up

Export Citation Format

Share Document