Antioxidant, Antibacterial, and Cytotoxic Activities of the EthanolicOriganum vulgareExtract and Its Major Constituents

Oregano is a perennial shrub that grows in the mountains of the Mediterranean and Euro/Irano-Siberian regions. This study was conducted to identify the major constituents of the ethanolicOriganum vulgareextract and examine the cytotoxic, antioxidant, and antibacterial properties of the extract but more importantly the contribution of its specific major constituent(s) or their combination to the overall extract biological activity. Gas chromatography/mass spectroscopy analysis showed that the extract contained monoterpene hydrocarbons and phenolic compounds, the major ones being carvacrol and thymol and to a lesser extent p-cymene, 1-octacosanol, creosol, and phytol. A549 epithelial cells challenged with the extract showed a concentration-dependent increase in cytotoxicity. A combination of thymol and carvacrol at equimolar concentrations to those present in the extract was less cytotoxic. The A549 cells pretreated with nonlethal extract concentrations protected against hydrogen-peroxide-induced cytotoxicity, an antioxidant effect more effective than the combination of equimolar concentrations of thymol/carvacrol. Inclusion of p-cymene and/or 1-octacosanol did not alter the synergistic antioxidant effects of the carvacrol/thymol mixture. The extract also exhibited antimicrobial properties against Gram-positive and Gram-negative bacterial strains including clinical isolates. In conclusion, the oregano extract has cytotoxic, antioxidant, and antibacterial activities mostly attributed to carvacrol and thymol.

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Antibacterial Activities of Soaps Formulated from Carapa procera Oil

Microbiology Research Journal International ◽

10.9734/mrji/2020/v30i730245 ◽

2020 ◽

pp. 119-125

Author(s):

Gbamelé Kouakou Kan Serges ◽

Yayé Yapi Guillaume ◽

Boyvin Lydie ◽

Bonouman Ira Ama Valerie ◽

Anigbé Amon Maurice ◽

...

Keyword(s):

Antibacterial Properties ◽

Antimicrobial Properties ◽

Antibacterial Activities ◽

Coli Strain ◽

Personal Hygiene ◽

Aureus Strain ◽

Bacterial Strains ◽

E Coli ◽

Skin Conditions ◽

Carapa Procera

Aims: The Carapa procera species is used of traditional for its antimicrobial properties especially for the skin. Its oil is used for various applications including the production of soaps used for personal hygiene and other skin conditions. The purpose of this study is to assess the antibacterial properties of soaps formulated from the oil of this species. Place and Duration of Study: The units of organic biological chemistry and structural organic chemistry of the UFR Structural Sciences of Matter and Technology (Université Félix Houphouet-Boigny) for the manufacture of soaps and the bacteriology unit of the Institut Pasteur de Côte d'Ivoire for bioassays between January 2019 and February 2020. Methodology: The antibacterial evaluation was carried out by the disc method on nutrient agars with determination of bacterial parameters (MIC and MBC). Discs of usuals antibiotics (Tetracycline, Rifampicin and Ampicillin) were used as a control to assess this activity as well as a commercially soap. Results: The inhibition diameters obtained from these different test soaps vary from 16.00 - 17.15 mm; 15.75 - 17.70 mm and 20.33 - 24.00 mm respectively on growth of the bacterial strains E. coli, P. aeruginosa and S. aureus. Unlike unscented soaps, scented soaps have the best inhibition diameter zones values. Moreover, the best MBC values (25 mg/mL) are obtained with the 2 soaps in fresh or aged state on the S. aureus strain against 50 mg/mL on the 2 other strains. In addition, with the exception of the E. coli strain which is resistant to Tetracycline, the 2 other strains are sensitive to all the antibiotics tested. In addition, this study also shows variability in the effect of soaps on these targets. Conclusion: Soaps formulated from Carapa procera oil do indeed possess antibacterial properties that could justify its usage against dermatoses in traditional medicine.

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Large-Scale Screening of 239 Traditional Chinese Medicinal Plant Extracts for Their Antibacterial Activities against Multidrug-Resistant Staphylococcus aureus and Cytotoxic Activities

Pathogens ◽

10.3390/pathogens9030185 ◽

2020 ◽

Vol 9 (3) ◽

pp. 185 ◽

Cited By ~ 1

Author(s):

Gowoon Kim ◽

Ren-You Gan ◽

Dan Zhang ◽

Arakkaveettil Kabeer Farha ◽

Olivier Habimana ◽

...

Keyword(s):

Large Scale ◽

Salvia Miltiorrhiza ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Multidrug Resistant ◽

Cytotoxic Activities ◽

Phenolic Contents ◽

Medicinal Plant Extracts ◽

Low Cytotoxicity

Novel alternative antibacterial compounds have been persistently explored from plants as natural sources to overcome antibiotic resistance leading to serious foodborne bacterial illnesses. In this study, the ethanolic extracts from 239 traditional Chinese medicinal plants (TCMP)’ materials were screened to discover promising candidates that have strong antibacterial properties against multidrug-resistant Staphylococcus (S.) aureus and low cytotoxicity. The results revealed that 74 extracts exhibited good antibacterial activities (diameter of inhibition zone (DIZ) ≥ 15 mm). Furthermore, 18 extracts (DIZ ≥ 20 mm) were determined their minimum inhibitory concentrations (MIC) and minimum bactericide concentrations (MBC), ranging from 0.1 to 12.5 mg/mL and 0.78 to 25 mg/mL, respectively. In addition, most of the 18 extracts showed relatively low cytotoxicity (a median lethal concentration (LC50) >100 µg/mL). The 18 extracts were further determined to estimate possible correlation of their phenolic contents with antibacterial activity, and the results did not show any significant correlation. In conclusion, this study selected out some promising antibacterial TCMP extracts with low cytotoxicity, including Rhus chinensis Mill., Ilex rotunda Thunb., Leontice kiangnanensis P.L.Chiu, Oroxylum indicum Vent., Isatis tinctorial L., Terminalia chebula Retz., Acacia catechu (L.f.) Willd., Spatholobus suberectus Dunn, Rabdosia rubescens (Hemsl.) H.Hara, Salvia miltiorrhiza Bunge, Fraxinus fallax Lingelsh, Coptis chinensis Franch., Agrimonia Pilosa Ledeb., and Phellodendron chinense C.K.Schneid.

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Antibacterial Effects of Oak Fruit, Jaft, and Jaftex Herbal Mouthwash: A Review

10.21203/rs.2.11341/v1 ◽

2019 ◽

Author(s):

Maria Cheraghi ◽

Fatemeh Babadi

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Web Of Science ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Antibiotic Sensitivity ◽

Antibacterial Activities ◽

Antibacterial Effects ◽

Digital Literature ◽

Review Study

Abstract Recently, natural products have been evaluated as a source of antimicrobial agent with efficacies against a variety of microorganisms. The antibacterial activities of the oak fruit,inner husk of oak fruit (Jaft), and Jaftex mouthwash have been studiedin several studies. Thisstudy aimedto review the studies ofthe effects of antibacterial properties of the oak fruit, oak fruit hull (Jaft), and Jaftex mouthwash. Materials and Methods In this review study, relevant articles related to the antimicrobial activity of the oak fruit, inner husk of oak fruit (Jaft), and 'Jaftex mouthwash' were searched from the current digital literature using electronic databases namely, SID, ScienceDirect, PubMed, Google Scholar, Magiran, Web of Science employing the same keywords from 1990 to 2019. Results Oakfruit, inner husk of oak fruit (Jaft), and Jaftex mouthwash have antimicrobial properties against many microorganisms. Discussion and Conclusion Antibacterial properties of oak fruit, inner husk ofoak fruit (Jaft) can be used to reverse the antibiotic sensitivity against pathogenic bacteria. Jaftex is recommended as an antibacterial and anti-plaque mouthwash.

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Antibacterial properties of an Indian traditional medicinal plant, Rhynchosia scarabaeoides (L.) DC. (Fabaceae)

Alternative Medicine Studies ◽

10.4081/ams.2011.e17 ◽

2011 ◽

Vol 1 (1) ◽

pp. 17 ◽

Cited By ~ 1

Author(s):

Surekha Challa ◽

Kiran K. Rajam ◽

Vishnu V. V. Satyanarayana Kasapu ◽

Suresh Kumar Tanneeru ◽

Venkata Siva Satyanarayana Kantamreddi

Keyword(s):

Ethyl Acetate ◽

Bacterial Infections ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Traditional Healers ◽

Diffusion Method ◽

Bacterial Strains ◽

Plant Parts ◽

Ethanol Extracts

Rhynchosia scarabaeoides (L.) DC plant parts are extensively used by traditional healers in India to treat a variety of bacterial diseases, such as dysentery, diarrohea and skin disorders. This article reports the antibacterial activities of n-hexane, ethyl acetate and ethanol extracts belonging to the leaf, stem and root parts of R. scarabaeoides against five bacterial strains, Bacillus subtilis, Escherichia coli, Klebseilla pneumonia, Proteus vulgaris and Staphylococcus aureus, using an agar gel diffusion method. The range of inhibition zone (IZ) was found to be 15-24 mm and the minimum inhibitory activity (MIC) was found to be 1 mL/well. The IZ was found to be higher in ethyl acetate extracts while this was moderate in ethanol extracts, and no activity was seen with n-hexane extracts or root extracts. The MIC value of leaf ethyl acetate extract was found to be 1 mg against bacterial strains P. vulgaris and S. aureus, whereas 2 mg was found against B. subtilis, K. pneumoniae and E. coli. These results support the traditional usage of R. scarabaeoides plant parts in the treatment of bacterial infections. Interestingly, this plant was screened for antibacterial activity for the first time and was found to be active. Detailed chemical investigations are, therefore, warranted.

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Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Molecules ◽

10.3390/molecules26154479 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4479

Author(s):

Pei-Jun Li ◽

Jiang-Juan Pan ◽

Li-Jun Tao ◽

Xia Li ◽

Dong-Lin Su ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Antibacterial Activities ◽

Synthesis Process ◽

Biological Synthesis ◽

X Ray Diffraction ◽

Aspergillus Japonicus ◽

Transmission Electron ◽

Naoh Solution

The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.

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Cu–Co Co-Doped Microporous Coating on Titanium with Osteogenic and Antibacterial Properties

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3120 ◽

2021 ◽

Vol 17 (7) ◽

pp. 1435-1447

Author(s):

Quan-Ming Zhao ◽

Bo Li ◽

Fu-Xun Yu ◽

Yan-Kun Li ◽

Jie-Shi Wu ◽

...

Keyword(s):

Clinical Performance ◽

Antibacterial Properties ◽

Microarc Oxidation ◽

Antimicrobial Properties ◽

Antibacterial Activities ◽

Bone Surgery ◽

Co Doping ◽

Proliferation And Differentiation ◽

Co Doped

Titanium (Ti) and its alloys are widely used in bone surgery by virtue of their excellent mechanical properties and good biocompatibility; however, complications such as loosening and sinking have been reported post-implantation. Herein we deposited a copper–cobalt (Cu–Co) co-doped titanium dioxide (TUO) coating on the surface of Ti implants by microarc oxidation. The osteogenic and antimicrobial properties of the coating were evaluated by in vitro experiments, and we also assessed β-catenin expression levels on different sample surfaces. Our results revealed that the coating promoted the adhesion, proliferation, and differentiation of MG63 osteoblasts, and TUO coating promoted β-catenin expression; moreover, the proliferation of Staphylococcus aureus was inhibited. To summarize, we report that Cu–Co co-doping can enhance the osteogenic and antibacterial activities of orthopedic Ti implants, leading to potentially improved clinical performance.

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ct-DNA Binding and Antibacterial Activity of Octahedral Titanium (IV) Heteroleptic (Benzoylacetone and Hydroxamic Acids) Complexes

International Journal of Medicinal Chemistry ◽

10.1155/2016/2361214 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11

Author(s):

Raj Kaushal ◽

Sheetal Thakur ◽

Kiran Nehra

Keyword(s):

Antibacterial Properties ◽

Antibacterial Activities ◽

Spectroscopic Technique ◽

Calf Thymus Dna ◽

Diffusion Method ◽

Bacterial Strains ◽

Intercalative Mode ◽

Heteroleptic Complexes ◽

Calf Thymus ◽

Ct Dna

Five structurally related titanium (IV) heteroleptic complexes, [TiCl2(bzac)(L1–4)] and [TiCl3(bzac)(HL5)]; bzac = benzoylacetonate; L1–5 = benzohydroximate (L1), salicylhydroximate (L2), acetohydroximate (L3), hydroxyurea (L4), and N-benzoyl-N-phenyl hydroxylamine (L5), were used for the assessment of their antibacterial activities against ten pathogenic bacterial strains. The titanium (IV) complexes (1–5) demonstrated significant level of antibacterial properties as measured using agar well diffusion method. UV-Vis absorption spectroscopic technique was applied, to get a better insight into the nature of binding between titanium (IV) complexes with calf thymus DNA (ct-DNA). On the basis of the results of UV-Vis absorption spectroscopy, the interaction between ct-DNA and the titanium (IV) complexes is likely to occur through the same mode. Results indicated that titanium (IV) complex can bind to calf thymus DNA (ct-DNA) via an intercalative mode. The intrinsic binding constant (Kb) was calculated by absorption spectra by using Benesi-Hildebrand equation. Further, Gibbs free energy was also calculated for all the complexes.

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SYNTHESIS, CHARACTERIZATION AND IN VITRO ANTIMICROBIAL EVALUATION OF SOME NOVEL BENZIMIDAZOLE DERIVATIVES BEARING HYDRAZONE MOIETY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s4.21328 ◽

2017 ◽

Vol 10 (16) ◽

pp. 1

Author(s):

Jayanta Sarma ◽

Gurvinder Singh ◽

Mukta Gupta ◽

Reena Gupta ◽

Bhupinder Kapoor

Keyword(s):

Antibacterial Properties ◽

Antimicrobial Properties ◽

Aromatic Aldehydes ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Benzimidazole Derivatives ◽

E Coli ◽

Antimicrobial Evaluation ◽

Antibacterial Potential

Objective: The synthesis of novel benzimidazole-hydrazone derivatives has been carried out based on the previous findings that both these pharmacophores possess potent antimicrobial activities. The antibacterial properties of synthesized derivatives were screened against both Gram-positive and Gram-negative bacteria.Methods: O-phenylenediamine on condensation with substituted aromatic acids in polyphosphoric acid gave benzimidazole nucleus which on reaction with ethyl chloroacetate and hydrazine hydrate in two different steps resulted in the formation of substituted acetohydrazides. The targeted compounds 6a-l were synthesized by reaction of substituted acetohydrazides with aromatic aldehydes and screened for their antibacterial potential by cup-plate method.Results: The synthesized benzimidazole-hydrazones exhibited moderate to strong antibacterial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The compounds 6a-6f were found to be most effective against S. aureus, E. coli, and P. aeruginosa. Among all the synthesized compounds, the zone of inhibition of 6f in highest concentration, i.e., 100 μg/ml were found to be >31 mm against all the stains of bacteria.Conclusion: The antibacterial results revealed that the synthetized derivatives have significant antimicrobial properties and further structure activity relationship studies may develop more potent and less toxic molecules.

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Synthesis of Copper Nanoparticles by Thermal Decomposition and Their Antimicrobial Properties

Journal of Nanomaterials ◽

10.1155/2014/980545 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 46

Author(s):

R. Betancourt-Galindo ◽

P. Y. Reyes-Rodriguez ◽

B. A. Puente-Urbina ◽

C. A. Avila-Orta ◽

O. S. Rodríguez-Fernández ◽

...

Keyword(s):

Thermal Decomposition ◽

Copper Nanoparticles ◽

Copper Chloride ◽

Metallic Copper ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Face Centered Cubic ◽

Bacterial Strains ◽

Tem Analysis

Copper nanoparticles were synthesized by thermal decomposition using copper chloride, sodium oleate, and phenyl ether as solvent agents. The formation of nanoparticles was evidenced by the X-ray diffraction and transmission electron microscopy. The peaks in the XRD pattern correspond to the standard values of the face centered cubic (fcc) structure of metallic copper and no peaks of other impurity crystalline phases were detected. TEM analysis showed spherical nanoparticles with sizes in the range of 4 to 18 nm. The antibacterial properties of copper nanoparticles were evaluatedin vitroagainst strains ofStaphylococcus aureusandPseudomonas aeruginosa. The antibacterial activity of copper nanoparticles synthesized by thermal decomposition showed significant inhibitory effect against these highly multidrug-resistant bacterial strains.

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Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Molecules ◽

10.3390/molecules26154589 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4589

Author(s):

Soumaya Touzani ◽

Hamada Imtara ◽

Shankar Katekhaye ◽

Hamza Mechchate ◽

Hayat Ouassou ◽

...

Keyword(s):

Cinnamic Acid ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antibacterial Properties ◽

Reducing Power ◽

Antibacterial Activities ◽

Total Phenolic ◽

Bacterial Strains ◽

Photodiode Array Detection

The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

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