The Effect of Pyrroloquinoline Quinone on the Expression of WISP1 in Traumatic Brain Injury

WISP1, as a member of the CCN4 protein family, has cell protective effects of promoting cell proliferation and inhibiting cell apoptosis. Although some studies have confirmed that WISP1 is concerned with colon cancer and lung cancer, there is little report about the influence of WISP1 in traumatic brain injury. Here, we found that the expression of WISP1 mRNA and protein decreased at 3 d and then increased at 5 d after traumatic brain injury (TBI). Meanwhile, immunofluorescence demonstrated that there was little colocation of WISP1 with GFAP, Iba1, and WISP1 colocalized with NeuN partly. WISP1 colocalized with LC3, but there was little of colocation about WISP1 with cleaved caspase-3. Subsequent study displayed that the expression ofβ-catenin protein was identical to that of WISP1 after TBI. WISP1 was mainly located in cytoplasm of PC12 or SHSY5Y cells. Compared with the negative control group, WISP1 expression reduced obviously in SHSY5Y cells transfected with WISP1 si-RNA. CCK-8 assay showed that pyrroloquinoline quinone (PQQ) had little influence on viability of PC12 and SHSY5Y cells. These results suggested that WISP1 played a protective role after traumatic brain injury in rats, and this effect might be relative to autophagy caused by traumatic brain injury.

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Catechins decrease neurological severity score through apoptosis and neurotropic factor pathway in rat traumatic brain injury

Zinc supplementation improves heme biosynthesis in rats exposed to lead ◽

10.18051/univmed.2017.v36.110-122 ◽

2017 ◽

Vol 36 (2) ◽

pp. 110

Author(s):

Retty Ratnawati ◽

Annisa Nurul Arofah ◽

Anastasia Novitasari ◽

Sartika Dewi Utami ◽

Made Ayu Hariningsih ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Severity Score ◽

Caspase 3 ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Caspase 8 ◽

Neurotropic Factor

BACKGROUND Catechins inhibits apoptosis through anti oxidant and anti inflamation pathway. Catechins also increases brain-derived neurotrophic factor (BDNF). There was a few research that explained the role of catechins in traumatic brain injury (TBI). The objective of the study was to evaluate the effect of catechins administration on neurologic severity score (NSS) through apoptosis and neurotropic pathway in traumatic brain injury rat model.METHODS A post test only controlled group design was performed using traumatic brain injury rat (Rattus novergicus) model through weight drop models. It was devided into negative control group, positive control group, TBI+catechins 513 mg/kgBW, TBI+catechins 926 mg/kgBW, TBI+catechins 1113 mg/kgBW. NSS was measured in the first hours, day three, and day seven. The expressions of NFkB, TNFa, Bcl-2, Bax, caspase 3, caspase 8, BDNF, and the numbers of apoptosis cells were evaluated by imunohistochemystry method. One way Anova and Kruskal Wwallis were used to analyse the data.Results TNFa, caspase 8, number of apoptosis cells were significantly decreased on the seventh day administration compared to the third day administration (p<0.05). Catechins increased the expression of Bcl-2/Bax and BDNF significantly (p<0.05). Yet, there were no significant differences between expression of caspase 3, NSS, Bcl-2/Bax ratio, and BDNF toward third days administration of catechins compared with seven days administration (p>0.050).Conclusions Administration of catechins decreased NSS through inhibiting inflammation and apoptosis, as well as induced the neurotrophic factors in rat brain injury. Catechins may serve as a potential intervention for TBI.

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THE PROTECTIVE ROLE OF OMEGA-3 AGAINST GENOTOXICITY AND REPRODUCTIVE TOXICITY OF COBALT OXIDE NANOPARTICLES ACUTE TREATMENT IN MALE MICE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i5.25245 ◽

2018 ◽

Vol 11 (5) ◽

pp. 423

Author(s):

Nahed A Hussien ◽

Hanan R. H. Mohamed

Keyword(s):

Cobalt Oxide ◽

Negative Control Group ◽

Protective Role ◽

Negative Control ◽

Control Group ◽

Omega 3 ◽

Genotoxic Potential ◽

Polychromatic Erythrocytes

Objective: Cobalt nanoparticles (NPs), especially cobalt oxide NPs (Co3O4 NPs) are attracting unique shaped NPs that are used in different biomedical applications and medicine. Different in vitro studies report their toxic and carcinogenic effect but limited in vivo studies were present on its genotoxic potential. The present study was aimed to evaluate the genotoxic potential of Co3O4 NPs on bone marrow cells and sperms and the protective role of omega-3 in male albino mice.Methods: Animals were segregated into four groups that were orally treated for 3 consecutive days, Group 1: Negative control; Group 2: Omega-3 (250 mg/kg); Group 3: Co3O4 NPs (20 mg/kg); and Group 4: Combined group (250 mg/kg Omega-3 and Co3O4 NPs 20 mg/kg).Results: The present results show that Co3O4 NPs administration significantly increased number of micronucleated polychromatic erythrocytes (PCEs)/1000 PCEs, sperm abnormalities, and DNA damage, significantly decreased sperm motility and concentration in comparison to negative control group. However, Omega-3 administration in the combined group modulates the genotoxic potential of Co3O4 NPs in comparison to Co3O4 NPs group.Conclusion: The present study reports the genotoxic potential of Co3O4 NPs in vivo and assesses the protective role of Omega-3 administration due to its antioxidant effect.

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BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s1.26613 ◽

2018 ◽

Vol 11 (13) ◽

pp. 225

Author(s):

Sulaeman A ◽

Patonah Patonah ◽

Negara Gg ◽

Negara Gg

Keyword(s):

Test Group ◽

Negative Control Group ◽

Protective Role ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

High Fat ◽

Adipose Tissues ◽

Obese Male

Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

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Chlorogenic acid prevents alcohol-induced brain damage in neonatal rat

Translational Neuroscience ◽

10.1515/tnsci-2017-0024 ◽

2017 ◽

Vol 8 (1) ◽

Cited By ~ 6

Author(s):

Zikang Guo ◽

Jiang Li

Keyword(s):

Oxidative Stress ◽

Cognitive Function ◽

Chlorogenic Acid ◽

Inflammatory Mediators ◽

Caspase 3 ◽

Negative Control Group ◽

Treated Group ◽

Negative Control ◽

Neonatal Rat ◽

Control Group

AbstractThe present investigation evaluates the neuroprotective effect of chlorogenic acid (CA) in alcohol-induced brain damage in neonatal rats. Ethanol (12 % v/v, 5 g/kg) was administered orally in the wistar rat pups on postnatal days (PD) 7-9. Chlorogenic acid (100 and 200 mg/kg, p.o.) was administered continuously from PD 6 to 28. Cognitive function was estimated by Morris water maze (MWM) test. However, activity of acetylcholinesterase, inflammatory mediators, parameters of oxidative stress and activity of caspase-3 enzyme was estimated in the tissue homogenate of cerebral cortex and hippocampus of ethanol-exposed pups. It has been observed that treatment with CA attenuates the altered cognitive function in ethanol-exposed pups. There was a significant decrease in the activity of acetylcholinesterase in the CA treated group compared to the negative control group. However, treatment with CA significantly ameliorates the increased oxidative stress and concentration of inflammatory mediators in the brain tissues of ethanol-exposed pups. Activity of caspase-3 enzyme was also found significantly decreased in the CA treated group compared to the negative control group. The present study concludes that CA attenuates the neuronal damage induced in alcohol exposed neonatal rat by decreasing the apoptosis of neuronal cells.

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ISONIAZID (INH)

The Professional Medical Journal ◽

10.29309/tpmj/18.4635 ◽

2018 ◽

Vol 25 (10) ◽

pp. 1587-1595

Author(s):

Umer Aleem ◽

Rahman Shah ◽

Noor Khan ◽

M. Suliman

Keyword(s):

Serum Bilirubin ◽

Statistical Significance ◽

Negative Control Group ◽

Protective Role ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Group Iii ◽

Group I ◽

Group 2

Objectives: Hepatotoxicity is the most complicated side effect of isoniazid (inh)in the patient treated for tuberculosis. In causes 8–30% hepatotoxicity in the developing world.Metabolism of INH produces a metabolite, called acetyl isoniazid. In this study hepatoprotectiveeffect of honey, in isoniazid induced animal model was assessed. Study Design: Randomizedcontrol trial. Setting: Saidu Medical College, Saidu Sharif Swat, KP. Period: October ToDecember 2017. Material and Methods: 40 healthy male rabbits were assigned randomly tothe group i, ii, iii and iv by using lottery method. Ten animals were grouped each row. Theisoniazid-induced hepatotoxic model was created by giving 50 mg inh/kg orally on daily basisfor eleven days. Group i was taken as negative control group ii as a positive control. Group iii andiv were experimental groups treated with 50 mg /kg/day and 100 mg /kg/day buckwheat honeyrespectively for eleven days. SPSS Version 16 software was used, mean, s.d. were determinedin all the groups. Values of serum bilirubin, sgpt, and alkaline phosphatase were comparedwith each other using pairt-test. Results: SGPT, Serum bilirubin, and alkaline phosphatasewere obtained in all the animals. Comparing group 1 negative control with group 2, 3 and 4shows statistical significance, (p=0.00). Comparing group 2 positive control with 3 and 4 showsstatistical significance, (p=0.00). Further comparing group 3 with group 4 also shows statisticalsignificance (p=0.00). Conclusion: From the above finding, it has been revealed that honeyhas got a protective effect in regressing hepatitis that has been induced in rabbit’s model byhigh doses of isoniazid. Related studies performed in which different chemicals and drugs havebeen tried for their protective role in isoniazid induced hepatitis also shows a similar type ofresults.

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Impact of bone-marrow-derived mesenchymal stem cells on adriamycin-induced chronic nephropathy

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2013-0503 ◽

2014 ◽

Vol 92 (9) ◽

pp. 733-743 ◽

Cited By ~ 7

Author(s):

Mohamed Sarhan ◽

Hanaa El Serougy ◽

Abdelaziz M. Hussein ◽

Mohamed El-dosoky ◽

Mohamed A. Sobh ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Survival Rate ◽

Caspase 3 ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Intravenous Injections ◽

Tubulointerstitial Damage

Objectives: To study the effects of bone-marrow-derived mesenchymal stem cells (BM-MSCs) on adriamycin (ADR)-induced chronic nephropathy in rats. Methods: 60 male Sprague–Dawley rats were distributed among 3 groups (20 rats each): (i) the negative control group, which was normal rats that received saline (vehicle); (ii) the positive control (ADR) group, which was rats that received 2 intravenous injections of ADR into the penile vein at 14 day intervals without treatment, and (iii) the MSC group, which were rats treated as for the ADR group that were also given 2 intravenous injections of MSCs (5 days after each ADR injection). Results: ADR caused a significant reduction in animal body mass, survival rate, hemoglobin (Hb) content, serum albumin, and renal GSH, and significantly increased serum levels of triglycerides, cholesterol, urinary protein excretion and kidney injury molecule-1 (KIM-1), renal MDA, as well as caspase-3 expression and glomerular and tubulointerstitial damage compared with the negative control group. MSC treatment failed to improve animal survival rate, body mass, Hb level, proteinuria, or hypoalbuminemia; however, it mildly improved the serum BUN, hyperlipidemia, caspase-3 expression, urinary levels of KIM-1, renal oxidative stress markers, and glomerular and tubulointerstitial damage score. Conclusion: administration of BM-MSCs during induction of ADR nephropathy provides partial protection, which could be due to improvements in the levels of of endogenous antioxidants, reduction of apoptosis, and maintenance of the integrity of the glomerular membrane.

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The Effects of Dexmedetomidine on Trauma-Induced Secondary Injury in Rat Brain

10.21203/rs.3.rs-580084/v1 ◽

2021 ◽

Author(s):

Ahmet Sen ◽

Basar Erdivanli ◽

Levent Tumkaya ◽

Huseyin Avni Uydu ◽

Tolga Mercantepe ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Rat Brain ◽

Caspase 3 ◽

Early Stage ◽

Additional Treatment ◽

Control Group ◽

Secondary Injury ◽

Sedative Drug ◽

Trauma Group

Abstract Secondary traumatic brain injury is a potentially modifiable and important determinator of the outcome. Sedation and analgesia are common components of the therapy. However current drug therapies have disadvantages like respiratory depression. The objective of this study was to investigate the effect of dexmedetomidine (Dex), a sedative drug with little to no depressive effect on respiratory centers, on secondary injury in rat brain tissue. Eighteen rats were randomized into three groups: Trauma group received anesthesia, followed by head trauma with Mild Traumatic Brain Injury Apparatus, the Trauma+Dex group received additional treatment of 100µg/kg intraperitoneal dexmedetomidine daily for three days, The Control group received anesthesia only. Malondialdehyde (MDA), glutathione (GSH), Na+, K+-ATPase, or sodium/potassium (Na/K-ATPase), and cysteine-aspartic proteases, cysteine aspartates-3 (caspase-3) levels were measured. MDA levels were highest in the Trauma group (p = 0.002 vs Control group). Mean levels in the Trauma+Dex group were lower, albeit still significantly high compared to the Control group (p = 0.002). Glutathione levels were similar in all groups (p = 0.99). Na/K-ATPase levels were lowest in the Trauma group, which is significant compared to the Control group (p = 0.002) and the Trauma+Dex group (p = 0.026). Histopathologic findings of tissue degeneration like edema, vascular congestion and neuronal injury, and cleaved caspase-3 levels were lower in the Trauma+Dex group compared with the Trauma group. Dexmedetomidine administered during the early stage of traumatic brain injury may inhibit caspase-3 cleavage. However, the mechanism does not seem to be related to the improvement of MDA or GSH levels.

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Intervention effect of fucoxanthin supplementation on cadmium-induced thyroid injury in mice

10.21203/rs.3.rs-21714/v1 ◽

2020 ◽

Author(s):

Haoyue Yan ◽

Ronge Xing ◽

Song Liu ◽

Pengcheng Li

Keyword(s):

Oxidative Stress ◽

Therapeutic Potential ◽

Day Treatment ◽

Pure Water ◽

Negative Control Group ◽

Negative Control ◽

Exposed Group ◽

Control Group ◽

Protective Effects ◽

Intervention Effect

Abstract Background: The Intervention effect of fucoxanthin, which is reportedly a powerful antioxidant, on cadmium-induced thyroid damage in mice was evaluated.Methods: Animals (N = 120) were divided into control group (given pure water, N=20) and CdCl-exposed group (given CdCl orally at a dose of 30 mg/kg body weight (bw)/day for 30 days, N=100). Besides, the CdCl-exposed group were divided into following 5 groups (N=20) to evaluate the intervention effect of fucoxanthin: 1) negative control group (NCG, animals were supplied with pure water); 2) positive control group (PCG, animals were supplied with 50 mg/kg bw/day thyroid tablets); 3) low fucoxanthin concentration group (F1, animals were supplied with 10 mg/kg bw/day fucoxanthin); 4)medium fucoxanthin concentration group (F2, animals were supplied with 25 mg/kg bw/day fucoxanthin); 5) high fucoxanthin concentration groups (F3, animals were supplied with 50 mg/kg bw/day fucoxanthin). A 14-day treatment was conducted for these animals. The levels of T4, T3, MDA, APX and CAT were measured, and the expression levels of Bax, Bcl-2, ERK1, ERK2, MEK1, eIf2α, p-eIf2α, GRP78 and GRP94 genes were determined using real-time reverse transcriptase-polymerase chain reactions (RT-PCR). In addition, tissue histopathology and ultrastructure were recorded and analyzed.Results: We found that injection of cadmium chloride (CdCl) decreased blood T4 and T3 levels to 27.10 ng/ml and 837.74 pg/ml, respectively. In addition, CdCl intoxication induced oxidative stress, structural abnormalities and apoptosis in thyroid follicles. Our results showed that treatment of CaCl-exposed mice with 25-50 mg/kg bw/day fucoxanthin appreciably decreased oxidative stress and apoptosis induced by CdCl, and restored the microstructural and ultrastructural organisation of the thyroid gland towards normalcy. Compared with the negative control group, fucoxanthin treatment groups showed significantly upregulated T4 and T3 levels (52.17 ng/ml and 1669.18 ng/ml, respectively; P ＜ 0.05), relieved oxidative stress by decreasing Malondialdehyde (MDA) level and increasing Catalase (CAT) and Ascorbate Peroxidase (APX) levels, and increased apoptosis inhibition through inhibiting the ERK1/2 pathway and preventing endoplasmic reticulum stress in thyroid follicular epithelial cells.Conclusion: Herein, our study provides evidence of the protective effects of fucoxanthin supplementation against thyroid damage, and suggests therapeutic potential of this pigment in cases of Cd intoxication and hypothyroidism.

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Effect of HylocereusPolyrhizus Rind Extract Toward Interleukin-1β, Vascular Endothelial Growth Factor Expression, Endometriosis Implant Area

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i08.9588 ◽

2017 ◽

Vol 9 (08) ◽

Author(s):

YanuarEka P. ◽

Hendy Hendarto ◽

Widjiati .

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Treatment Group ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Vascular Endothelial ◽

Mice Model ◽

Endothelial Growth Factor ◽

Fruit Rind

Retrograde menstruation lead to I Kappa B Kinase (IKK) fosforilation in peritoneum macrophage and cause secretion of proinflammatory cytokine interleukin1β then stimulate endometriosis cell to produce Vascular Endothelial Growth Factor which lead to increasing of endometriosis lession seen as endometriosis implant area. Cytokine secretion was inhibited through prevention of NF-κB activation by dragon red fruit rind extract (Hylocereuspolyrhizus). The aim of this reserach is to know the effect of dragon red fuit rind extract with 0,25; 0,5; and 1 mg/g bodyweight dosage toward IL-1β, VEGF expression and implant area in endometriosis mice model. The design of this experiment was randomized post test only control group design.Endometrios mice model were made in 14 days and split into two group, positive control group and treatment group after two week negative control group and postive control group were given Na-CMC 0,5% solution consequetively, and treatment group were given dragon red fruit extract with different dosage. Signification number for IL-1β is p>0,05, signification number for VEGF is p>0,05, and implant area signification number is p>0,05. Administration of dragon red fruit rind extract can decrease IL-1β, VEGF, and implant area.

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The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽

10.30649/denta.v12i1.162 ◽

2018 ◽

Vol 12 (1) ◽

pp. 34

Author(s):

Arya Barahmanta ◽

Muhammad Faizal Winaris ◽

Pambudi Raharjo

Keyword(s):

Hyperbaric Oxygen ◽

Oxygen Therapy ◽

Tooth Movement ◽

Bone Remodelling ◽

Orthodontic Tooth Movement ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Pressure Area

Background: Orthodontic tooth movement is a interaction prosess of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO). Purpose: To determine the difference number of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. Materials and Methods: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male) were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. Result: The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest than positive control group (K+=3,83) and treatment (P=3,25). Conclusion: Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.<pre> </pre>Keywords: Hyperbaric Oxygen, Tooth movement, Bone remodeling, Osteocyte Correspondence: Arya Brahmanta, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: <a href="mailto:[email protected]">arya.brahmanta@hangtuah.ac.id</a>

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