scholarly journals Antibacterial Effect of Hydroalcoholic Extract ofPunica granatumLinn. Petal on Common Oral Microorganisms

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Farnaz Hajifattahi ◽  
Elham Moravej-Salehi ◽  
Maryam Taheri ◽  
Arash Mahboubi ◽  
Mohammad Kamalinejad
Keyword(s):  
Growth Inhibition ◽  
Antibacterial Effect ◽  
Brain Heart Infusion ◽  
Inhibition Zone ◽  
Maximum Effect ◽  
Streptococcus Sobrinus ◽  
Bacterial Strains ◽  
Hydroalcoholic Extract ◽  
Growth Inhibition Zone

Objectives. This study aimed to assess the effect of hydroalcoholic extract ofPunica granatumLinn. (P. granatum) petal onStreptococcus sanguinis,Streptococcus mutans,Streptococcus salivarius,Streptococcus sobrinus,andEnterococcus faecalis.Materials and Methods. In this in vitro study,P. granatumextract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX), and ampicillin was evaluated on brain heart infusion agar (BHIA) using the cup-plate method. By assessing the diameter of the growth inhibition zone, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were determined for the above-mentioned bacteria.Results. Hydroalcoholic extract ofP. granatumpetal had inhibitory effects on the proliferation of all five bacterial strains with maximum effect onS. mutanswith MIC and MBC of 3.9 mg/mL. The largest growth inhibition zone diameter belonged toS. sanguinisand the smallest toE. faecalis. Ampicillin and CHX had the greatest inhibitory effect onS. sanguinis.Conclusions. Hydroalcoholic extract ofP. granatumhad a significant antibacterial effect on common oral bacterial pathogens with maximum effect onS. mutans, which is the main microorganism responsible for dental plaque and caries.

scholarly journals KEEFEKTIFAN EKSTRAK MENGKUDU PADA BERBAGAI KONSENTRASI TERHADAP PENGHAMBATAN PERTUMBUHAN BAKTERI RALSTONIA SP. SECARA IN VITRO

2004 ◽  
Vol 4 (2) ◽  
pp. 83-88
Author(s):  
Efri Efri ◽  
Titik Nur Aeny
Keyword(s):  
Growth Inhibition ◽  
Growth Inhibitor ◽  
Inhibition Zone ◽  
Morinda Citrifolia ◽  
In Vitro Growth ◽  
Fruit Extract ◽  
Bacterial Wilt Disease ◽  
Growth Inhibition Zone ◽  
Medicinal Properties

Effectivity of  Morinda citrifolia extract on in-vitro growth inhibition of Ralstonia sp.  Morinda citrifolia has been known as one of plants having numerous medicinal properties.  Extract of the fruits shows antibacterial activity against several bacteria including Pseudomonas aeruginosa.  The purpose of this study was to observe inhibitor effect of  M. citrifolia fruit extract, and to investigate the effect of different concentrations of  M. citrifolia fruit extract on in-vitro growth of  Ralstonia (Pseudomonas) sp., the causal agent of banana bacterial wilt disease in Lampung.  Inhibition effect of  M. citrifolia was determined by the presence of inhibition zone, and concentration effect was determined by the diameter of  inhibition zone . The study consisted of two experiments.  The treatments of the first experiment were three different kinds of fruit extract, namely the whole fruit, the seeds, and the fleshy parts, and the second one consisted of six different levels of extract concentrations.  The results showed that the whole fruit extract was the most effective as an in-vitro growth inhibitor, and the higher the concentration level the higher the growth inhibition zone formed.

scholarly journals Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Lucía Arévalo-Híjar ◽  
Miguel Ángel Aguilar-Luis ◽  
Stefany Caballero-García ◽  
Néstor Gonzáles-Soto ◽  
Juana Del Valle-Mendoza
Keyword(s):  
Enterococcus Faecalis ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Inhibition Zone ◽  
Bactericidal Effect ◽  
Growth Inhibition Zone ◽  
Methanolic Extracts

Objective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results. The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions. In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.

scholarly journals Infestation of tall fescue (Festuca arundinacea Schreb.) with Neotyphodium coenophialum and its influence on growth of chosen microorganisms in vitro

2012 ◽  
Vol 58 (2) ◽  
pp. 369-380 ◽  
Author(s):  
Dariusz Pańka
Keyword(s):  
Growth Inhibition ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Inhibition Zone ◽  
Bipolaris Sorokiniana ◽  
Gaeumannomyces Graminis ◽  
Neotyphodium Coenophialum ◽  
Mycelium Growth ◽  
Growth Inhibition Zone

Occurrence of <i>Neotyphodium coenophialum</i> in tall fescue cultivars cultivated in Poland and determination an endophyte inhibition effect on mycelium growth of chosen microorganisms <i>in vitro</i> were investigated. Seventeen seed lots of 11 cultivars of tall fescue were examined. The endophyte mycelium was dyed with bengal rose and microscopically examined to detect <i>N. coenophialum</i>. Occurrence of endophyte was checked with PCR method. Influence of endophyte on growth of 15 microorganisms was established in the laboratory conditions on Petri dishes with PDA medium at 10, 20 and 30<sup>°</sup>C. <i>Neotyphodium coenophialum</i> occurred only in two seed lots, 'Barrocco' - 42% and Terros - 2%. Living mycelium of endophyte was isolated only from 'Barrocco'. The highest mycelium growth inhibition of <i>Bipolaris sorokiniana</i>, <i>Fusarium avenaceum</i>, <i>F. equiseti</i>, <i>Microdochium nivale</i> and <i>Gaeumannomyces graminis</i> by endophyte at 30<sup>°</sup>C was recorded. The highest width of growth inhibition zone (4mm) was detected for the last pathogen. Mycelium growth of <i>B. sorokiniana</i> and <i>M. nivale</i> was not inhibited at 10<sup>°</sup>C, and for <i>F. avenaceum</i> at 10 and 20<sup>°</sup>C.

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone 

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

scholarly journals In Vitro Antibacterial and Cytotoxicity Assessments of an Orthodontic Bonding Agent Containing Benzalkonium Chloride

2009 ◽  
Vol 79 (2) ◽  
pp. 331-337 ◽  
Author(s):  
Kayo Saito ◽  
Tohru Hayakawa ◽  
Rihito Kawabata ◽  
Daijiro Meguro ◽  
Kazutaka Kasai
Keyword(s):  
Antibacterial Activity ◽  
Benzalkonium Chloride ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Streptococcus Sobrinus ◽  
Disk Diffusion Method ◽  
Gingival Cells ◽  
Orthodontic Bonding

Abstract Objective: To assess the antibacterial activity and cytotoxicity of an orthodontic bonding material containing an antibacterial agent. Materials and Methods: Superbond C&B (4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane [4-META/MMA-TBB]) resin was mixed with benzalkonium chloride (BAC) to obtain final BAC concentrations of 0.25%, 0.75%, 1.25%, 1.75%, 2.5%, and 5.0% (wt/ wt). Antibacterial activity against Streptococcus mutans and Streptococcus sobrinus was evaluated by soaking the BAC-resin in distilled water at 37°C for periods of 30, 90, and 180 days. Antibacterial activity of the BAC-resin was measured by the disk diffusion method, and the inhibition zone around each sample was measured and recorded. For evaluation of cytotoxicity, BAC-resin samples were put into cell culture inserts placed above human gingival cells and were incubated at 37°C for 1, 3, and 6 days. Cytotoxicity was assessed with a tetrazolium bromide reduction assay. Results: The antibacterial activity of BAC-incorporated resin samples decreased significantly after immersion in water for 180 days, regardless of BAC concentration. The antibacterial activity of nonimmersed resin containing 0.25% or 1.75% BAC was comparable with that of 5.0% BAC-resin immersed for 180 days. In cytotoxicity tests, most cells died when exposed to resins containing 1.75%, 2.5%, and 5% BAC. No difference was observed between resins containing 0.25% and 0.75% BAC at 1, 3, and 6 days of culture. Conclusions: The addition of BAC to 4-META/MMA-TBB resin confers an antibacterial effect even after immersion in water, and 4-META/MMA-TBB resin containing 0.25% to 0.75% BAC has no significant cytotoxic effect.

scholarly journals Evaluation of the Antimicrobial Effect of Mineral Trioxide Aggregate Mixed with Fluorohydroxyapatite against E. faecalis In Vitro

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Behnam Bolhari ◽  
Aidin Sooratgar ◽  
Maryam Pourhajibagher ◽  
Nazanin Chitsaz ◽  
Iman Hamraz
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Mineral Trioxide Aggregate ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Statistical Analyses ◽  
Biofilm Inhibition ◽  
Growth Inhibition Zone

Enterococcus faecalis is the dominant microorganism in chronic apical periodontitis. It is more resistant to local antiseptic agents than other endodontic microorganisms. Currently, mineral trioxide aggregate (MTA) is considered as an ideal material in many endodontic procedures. Some studies have shown that MTA has good antibacterial activity against E. faecalis. However, some studies have investigated the effect of incorporating some materials into MTA on its antibacterial activity against E. faecalis. No study has evaluated the effect of incorporating fluorohydroxyapatite nanoparticles (nano-FHA) on the antimicrobial activity of MTA. Therefore, the present study evaluated the antimicrobial effect of MTA mixed with nano-FHA on E. faecalis in vitro. The study was carried out on 18 samples in three groups: pure MTA, MTA mixed with 10 wt% of nano-FHA, and MTA mixed with 15 wt% of nan-FHA. The effect of nano-FHA on the antibacterial activity of MTA on E. faecalis was evaluated by evaluating the growth inhibition zone around each sample. The antimicrobial effect of samples on inhibiting E. faecalis biofilm formation and inhibiting microbial growth of E. faecalis in the planktonic phase was evaluated by disk agar diffusion (DAD), biofilm inhibition assay (BIA), and direct contact assay (DCA) tests, respectively. All the above tests were analyzed after 24 and 72 hours. Factorial designs were used for statistical analyses. Tukey tests were used for two-by-two comparisons. All the statistical analyses were carried out with SPSS 26. DAD results showed no formation of the growth inhibition zone in all the samples after 24 and 72 hours. The microbial colony counts in the BIA and DCA tests in the groups modified with FHA nanoparticles were significantly lower than the pure MTA group ( P < 0.05 ). The microbial colony counts increased in all the groups over time ( P < 0.05 ). Incorporating nano-FHA into MTA improved the antimicrobial activity of MTA against E. faecalis compared to pure MTA. The highest antimicrobial activity was achieved after incorporating 15 wt% of nano-FHA into MTA at the 72-hour interval.

scholarly journals Influence of base on the release of antibiotics from officinal ointments

2005 ◽  
Vol 62 (5) ◽  
pp. 383-387
Author(s):  
Marija Toskic-Radojicic ◽  
Zorka Nonkovic
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Lipid Analysis ◽  
Inhibition Zone ◽  
Aureus Strain ◽  
Growth Inhibition Zone ◽  
Erythromycin Base ◽  
Thermotropic Liquid Crystals ◽  
Agar Diffusion Test

Backgraund. Officinal basis for the antibiotic ointments according to the 4th Yugoslav Pharmacopoeia is a hydrophobic base containing only aliphatic hydrocarbons. The fact that antibiotics are predominantly not lipophylic raises the question about the suitability of that particular type of the base for the manufacturing of antibiotic ointments. Recent studies of the lipid analysis of the skin corneal layer indicated that lipids had shown the bilamellar organization in the skin intercorneal space. Such structural organization could be seen in the ambiphylic bases whose structure was based on carefully selected emulsifier couple, consisting of the lyotropic and thermotropic liquid crystals. The aim of this study was to test the velocity of antibiotics (klindamycin hydrochloride, erythromycin base and chloramphenicol) release from the hydrophobic ointment-type bases, and from ambiphylic bases of anionic and non-ionic types. Methods. Membrane-free agar diffusion test as the basic method for testing the release velocity in vitro and Staphylococcus aureus as the strain highly susceptible to the chosen antibiotics were used. All the analyzed samples were manufactured as the suspension-type ointments. Results. The highest growth inhibition zone of the Staphylococcus aureus strain for all three analyzed antibiotics was achieved from the non-ionogenic ambiphylic base; the clear growth inhibition zone area for Staphylococcus aureus strain in the preparations containing anionic ambiphylic base was smaller by 10-31.28%, and in the preparations containing aliphatic hydrocarbon-type base, the decrease was 11.46-31.28%, compared to the results achieved with the non-ionogenic ambiphylic base. Conclusion. The optimal release velocity for the analyzed antibiotics was achieved from the non-ionic ambiphylic base.

New manufacturing process to develop antibacterial dyed polyethylene terephthalate sutures using plasma functionalization and chitosan immobilization

2021 ◽  
pp. 152808372110505
Author(s):  
Nesrine Bhouri ◽  
Faten Debbabi ◽  
Abderrahmen Merghni ◽  
Esther Rohleder ◽  
Boris Mahltig ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Terephthalate ◽  
Manufacturing Process ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
The United States ◽  
Mechanical Tests ◽  
Inflammatory Reactions ◽  
Plasma Functionalization

The main purpose of this paper is to develop a new manufacturing process leading to have antibacterial dyed non-absorbable braided polyethylene terephthalate (PET) sutures using biocompatible and non-toxic products. This manufacturing process allows better visibility of sutures in the surgical field and reduces the risk of infections and inflammatory reactions without affecting the mechanical properties while meeting the United States Pharmacopeia (USP) requirements. Plasma functionalization, acrylic acid (AA) grafting, and bioactive chitosan (CH) coating were used before the dyeing process with a biocompatible non-toxic acid dye, approved by the Food and Drug Administration (FDA). The influence of experimental parameters on the suture properties and the K/S values of the dyed sutures are investigated. Infrared spectroscopy confirms the presence of new bonds to immobilize chitosan on the surface of the suture. Mechanical tests confirm that the mechanical properties of sutures have not been affected. The in vitro antibacterial effect of dyed PET sutures showed an inhibition zone of 11 mm against S. aureus, 4 mm against P. aeruginosa, and 1 mm against E. coli. This study reveals that the new finishing process of sutures is a promising method to achieve an antibacterial effect with a uniform shade and smooth surfaces.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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